Following Ariadne's thread: a new perspective on RBR ubiquitin ligases
© Wenzel and Klevit; licensee BioMed Central Ltd. 2012
Received: 5 December 2011
Accepted: 15 March 2012
Published: 15 March 2012
Ubiquitin signaling pathways rely on E3 ligases for effecting the final transfer of ubiquitin from E2 ubiquitin conjugating enzymes to a protein target. Here we re-evaluate the hybrid RING/HECT mechanism used by the E3 family RING-between-RINGs (RBRs) to transfer ubiquitin to substrates. We place RBRs into the context of current knowledge of HECT and RING E3s. Although not as abundant as the other types of E3s (there are only slightly more than a dozen RBR E3s in the human genome), RBRs are conserved in all eukaryotes and play important roles in biology. Re-evaluation of RBR ligases as RING/HECT E3s provokes new questions and challenges the field.
E3 ligases: historically a two-party system
Eukaryotic E3 ubiquitin ligases are generally identified by the presence of either a HECT or a RING domain. The features of each type of domain are well defined and are readily predictable by primary sequence analysis. RINGs are characterized by a regular spacing of conserved cysteines and histidines which bind two Zn2+ ions that stabilize the overall structure of this domain, allowing for recognition and activation of E2 Ub-conjugating enzymes . HECT domains are identified on the basis of their similarity to the founding member of the family, E6AP. In contrast to RING domains, which can occur at any position within a given protein, all known HECT domains are found at the carboxy-terminal end of their respective proteins. The HECT domain has a bilobal structure: the lobe at the amino-terminal end of the domain (the N-lobe) serves as the E2-binding domain, and the lobe at the carboxyl terminus (the C-lobe) contains the catalytic cysteine.
There are two general mechanisms by which the ultimate substrate-ubiquitin isopeptide adduct is formed. An essential difference between the two mechanisms is the location of activated ubiquitin at the final transfer step (Figure 1a). In reactions involving RING/UBOX-type ligases, the ubiquitin is attached to an E2 to form an E2~Ub thioester conjugate, and the E3 binds both substrate and the E2~Ub simultaneously to promote the aminolysis reaction in which ubiquitin is transferred to a lysine on a substrate. By an as yet undetermined mechanism, E3 binding enhances the reactivity of the E2~Ub thioester bond to allow for aminolysis [3, 4]. Catalytic residues have not been identified for RING/UBOX type ligases and are presumed not to exist. In reactions involving HECT E3s, ubiquitin is transferred from an E2~Ub to form an E3~Ub thioester conjugate and the final transfer step occurs directly from the E3 active site to a substrate lysine. Thus, the two mechanisms differ in terms of the identity of the active site that is responsible for the aminolysis: it is the E2 active site in RING/UBOX-catalyzed reactions and it is the E3 active site in HECT-catalyzed reactions. Substrates may be mono-ubiquitinated at one or more sites; or poly-ubiquitin chains may be attached to them. Poly-ubiquitin chains may be of eight known topologies determined by their distinct linkages, and named K48, K63, K11, K27, K29, K33, and K6 chains according to the lysine residue through which the ubiquitins are linked to one another; or linear chains when the linkage is between the carboxyl terminus of one ubiquitin and the amino terminus of the next
Because the E2 active site is responsible for aminolysis in RING/UBOX-catalyzed reactions, it follows that the product produced by RING/UBOX-type ligases, be it mono-ubiquitination or a poly-ubiquitin chain of a specific topology, is determined in large part by the identity of the E2 involved in the reaction. A RING-type E3 that can bind a diverse set of E2s has the potential to produce several distinct types of ubiquitination products. This phenomenon has been demonstrated for a growing number of RING-type ligases such as BRCA1/BARD1 and the APC, which can produce poly-ubiquitin of K63 and K48 linkages, as well as mono-ubiquitin, depending on the E2 [5, 6]. In contrast, it is the identity of the HECT-type E3 itself that determines the type of ubiquitin modifications conferred on substrates by this class of E3 ligases, and residues in the active-site-containing C-lobe have been shown to determine the type of ubiquitination signal generated by HECT-type E3s .
RBR E3s: Reaching across the aisle
RBR E3s were originally identified by virtue of their RING domains, but the presence of two additional domains, IBR and RING2, define them as a subclass [8, 9]. RBR-type ligases are defined by a trio of closely spaced domains: 1) a canonical amino-terminal RING domain, dubbed RING1, 2) an in-between RING domain (IBR), and 3) a domain named RING2 . As in the case of canonical RING domains, RBR domains are not limited to any particular location within the proteins that contain them. Although RING1 domain sequences follow the cysteine and histidine patterns typical of RINGs from other E3 ligase families, the IBR and RING2 domain do not, and these two domains are unique to RBR-type proteins. RBR E3 ligases are found throughout eukaryotes with two members in yeast and thirteen in human .
Despite their strong persistence throughout evolution, most RBR E3s are not well understood, and their substrates and E2 partners are poorly defined. Members of this E3 family mediate diverse processes that include the regulation of translation and the activation of NF-κB signaling, among others [11, 12]. The most studied RBR E3 is Parkin, because of its association with Parkinson's disease . The list of Parkin substrates continues to grow, although it is not clear which are critical for understanding the death of dopaminergic neurons, the hallmark of Parkinson's disease. The first substrates reported for Parkin were α-synuclein, Pael-R, and CDCrel-1, all of which accumulate in patients with heritable Parkinson's disease [14–16]. These substrates suggest a role for Parkin in the clearance of misfolded or aggregation-prone proteins. Additionally, Parkin is recruited to mitochondria where it is thought to regulate turnover of damaged mitochondria. Proteins that regulate mitochondrial morphology, mitofusin-1 and mitofusin-2, are substrates of Parkin ubiquitination [17, 18].
Several RBR ligases have roles in regulating immune signaling. Overexpression of RNF216 (TRIAD3, ZIN) enhances the degradation of specific Toll-like receptors, with co-ordinate down-regulation of Toll-like receptor signaling . RNF216 may function to attenuate the host response to viral invasion, as RNF216 ubiquitinates TRAF3, a potentiator of the anti-viral response, and targets it for proteasomal degradation . The RBR proteins Rbck1 (HOIL-1) and RNF31 (HOIP) form a heterodimeric E3 ligase that function together with the protein SHARPIN to generate linear ubiquitin chains [21, 22]. Linearly-linked poly-ubiquitin chains specifically recruit components of the NF-κB signaling pathway including NEMO to promote the phosphorylation and subsequent degradation of inhibitors of NF-κB.
Yeast contain two RBR proteins, orthologs for Ariadne (ARIH1) and RNF14 (ARA54). It has been suggested that the extreme conservation of RBR genes from the earliest eukaryotes to human may indicate that these proteins assume a housekeeping role . Human RNF14 was first identified through a yeast two hybrid screen for proteins that bind to the androgen receptor, and is thought to be a co-activator of androgen receptor function . The yeast ortholog to RNF14 may have a function in the regulation of translation termination . The Drosophila protein Ariadne, which was the first member of the RBR family to be described, is important for the development of the fly nervous system: neuronal differentiation is disrupted in Ariadne mutants because of a failure in axon guidance . Ariadne was named after the Greek goddess Ariadne, whose thread was said to help guide Theseus out of the Minotaur's labyrinth.
Ariadne and other RBR-type E3s have been presumed to be RING-like in both structure and mechanism because of the similarities between RING1 and canonical RING domains. Unexpectedly, we discovered that the human RBR E3 HHARI (human homolog of Ariadne) functions in a way that is analogous to that of a HECT-type ligase, in that it forms an obligate thioester bond with ubiquitin before it is transferred to the substrate  (Figure 1b). A cysteine in the RING2 domain that is highly conserved among all RBR-type E3s functions like a HECT active site cysteine: when this cysteine is mutated to serine, an oxy-ester-linked HHARI~Ub intermediate can be trapped. Mutation of the analogous cysteine residue (C431) in RING2 of Parkin disrupts ubiquitin transfer mediated by this RBR E3, suggesting that this mode of ubiquitin transfer is a general feature of RBR E3s. Because they contain both a RING-type E2-binding domain at their amino-terminal end and an active-site-containing domain at their carboxy-terminal end, we proposed that RBRs can be thought of as RING-HECT hybrids.
Domain and structural analysis of RBRs
To date, structural insights into the RBR ligase supradomain are limited to structures solved for individual component domains: there are two structures of IBR domains, and one structure each of a RING1 and RING2 domain [26, 27] [PDB ID 1WIM, PDB ID 2CT7]. The dearth of structural information is probably due to the difficulty of expressing and purifying these proteins in large amounts. With such a small sampling, it is unclear whether these structures will be representative of the entire family of RBR ligases. However, the sequence similarity of these domains among human members of the RBR ligase family suggests we may expect to see similar structures.
RBR isopeptide catalysis
The observation that RBR ligases function through a HECT-like mechanism implies that there are additional residues near the active site cysteine that facilitate isopeptide catalysis with a substrate lysine. However, if studies with HECT E3s are any indication, these may be difficult to identify. Aside from the active site cysteine, few catalytic residues have been identified within HECT domain ligases. Studies involving a bacterial HECT-like E3, SopA, identified a conserved motif among HECT domain active sites surrounding the active site cysteine, LXXShTCfXn (where upper case letters indicate invariant residues, lower case letters indicate conserved residues and X indicates a variable position) . Mutation of the conserved leucine or threonine to an alanine decreases activity . Removal of a highly conserved phenylalanine in the carboxy-terminal tail of HECT domain ligases abolishes the ability of HECT E3s to modify lysines on substrates, but does not interfere with transthiolation from E2 to the HECT active site cysteine . Whether the conserved phenylalanine in HECT domains plays a direct role in catalysis remains to be seen. It is not clear if RBR-type E3s will use a similar mechanism for function. The lack of similarity between HECT E3 C-lobes and RBR RING2s at the sequence level suggests it will be challenging to identify residues that are important for isopeptide catalysis on the basis of our current knowledge. Residues around the RBR active site cysteine do not follow the LXXShTCfXn motif, and as the role of the carboxy-terminal phenylalanine in HECT-type ligases is not well understood, it is impossible to know if RBR-type ligases have residues that fulfill a similar function. The possibility remains that residues in other domains of the RBR may contribute to the activity of RING2. Structural and biochemical characterization of RING2 in context of the RING1 and IBR domains may be key to future progress in this respect.
Like HECT ligases, the ubiquitination products generated by RBRs are independent of E2 identity and are likely to be an intrinsic property of each RBR E3. To date, polyubiquitin chains with linear, Lys48, and Lys63 linkages, multiple mono-ubiquitination, and mono-ubiquitination have all been reported as products of RBR ligases [37–41]. Although Parkin itself has been reported to form many types of products, a recent study suggests that Parkin products can be influenced by the fusion of artificial molecular tags, or truncation of constructs, so previous results should be viewed in this light . The RBR proteins RBCK1 (HOIL-1) and RNF31 (HOIP) form a complex known as LUBAC (Linear Ubiquitin Assembly Complex) and produce linear polyubiquitin chains with the human E2s UbcH7, UbcH5, and Ube2k . To date, no HECT-type or canonical RING-type E3s have been reported to build linear poly-ubiquitin chains, suggesting that this property may be distinctive of RBR E3s. Future studies aimed at understanding the determinants within RBRs that specify the type of product generated are needed to further our understanding of RBR mechanism and function.
The distinct features of the RBRs indicate that attempts to extrapolate from knowledge of the other better characterized classes of E3 ligases are likely to fail. This opens a host of unanswered questions regarding RBR function. Which E2s work to transfer ubiquitin to RBR E3s and what are the determinants and features of this step? To date, the cohort of E2s identified as working with RBR-type ligases most often includes the E2s UbcH7 and UbcH8 . UbcH7 (and probably UbcH8 by analogy) can only transfer ubiquitin via a transthiolation reaction and are therefore specialized E2s for HECT-type transfer mechanisms . Even though it is a RING domain (RING1) of RBRs that binds E2s directly, the RBR version of the domain appears to bind E2s with higher affinity than do canonical E3 RING domains: complexes of UbcH7 and RING1s can be identified by pull-down and co-immunoprecipitation experiments . What are the determinants and consequences of stronger E2 binding and what features of UbcH7 and UbcH8 makes them preferred RBR-binding partners? How do RBR ligases recognize and bind substrates? Substrate binding has been reported for almost every domain from RING1 to RING2 and in regions outside the RBR domain . Given the variety of substrate binding modes documented for other families of E3 ligases, it seems unlikely that RBR ligases will have a common mechanism for substrate binding. The RBR ligase Parkin has been found to associate with SCF-type ubiquitin ligases, raising the possibility that adapter proteins may provide substrate specificity for a subset of these E3s . Future studies informed by the new insights regarding the distinctive mode of action for RBR E3s will no doubt shed light on these and other questions surrounding RBR function.
We thank P Brzovic, J Pruneda, and K Dove for critical reading and comments. We acknowledge support from the National Institute of General Medical Sciences in the form of 5R01 GM088055 (REK).
- Li W, Bengtson MH, Ulbrich A, Matsuda A, Reddy VA, Orth A, Chanda SK, Batalov S, Joazeiro CA: Genome-wide and functional annotation of human E3 ubiquitin ligases identifies MULAN, a mitochondrial E3 that regulates the organelle's dynamics and signaling. PLoS One. 2008, 3: e1487-10.1371/journal.pone.0001487.PubMedPubMed CentralView Article
- Deshaies RJ, Joazeiro CA: RING domain E3 ubiquitin ligases. Annu Rev Biochem. 2009, 78: 399-434. 10.1146/annurev.biochem.78.101807.093809.PubMedView Article
- Wenzel DM, Lissounov A, Brzovic PS, Klevit RE: UBCH7 reactivity profile reveals parkin and HHARI to be RING/HECT hybrids. Nature. 2011, 474: 105-108. 10.1038/nature09966.PubMedPubMed CentralView Article
- Ozkan E, Yu H, Deisenhofer J: Mechanistic insight into the allosteric activation of a ubiquitin-conjugating enzyme by RING-type ubiquitin ligases. Proc Natl Acad Sci USA. 2005, 102: 18890-18895. 10.1073/pnas.0509418102.PubMedPubMed CentralView Article
- Christensen DE, Brzovic PS, Klevit RE: E2-BRCA1 RING interactions dictate synthesis of mono- or specific polyubiquitin chain linkages. Nat Struct Mol Biol. 2007, 14: 941-948. 10.1038/nsmb1295.PubMedView Article
- Roderigo-Brenni MC, Morgan DO: Sequential E2s drive polyubiquitin chain assembly on APC targets. Cell. 2007, 130: 127-139. 10.1016/j.cell.2007.05.027.View Article
- Kim HC, Huibregtse JM: Polyubiquitination by HECT E3s and the determinants of chain type specificity. Mol Cell Biol. 2009, 29: 3307-3318. 10.1128/MCB.00240-09.PubMedPubMed CentralView Article
- van der Reijden BA, Erpelinck-Verschueren CA, Löwenberg B, Jansen JH: TRIADs: a new class of proteins with a novel cysteine-rich signature. Protein Sci. 1999, 8: 1557-1561. 10.1110/ps.8.7.1557.PubMedPubMed CentralView Article
- Aguilera M, Oliveros M, Martínez-Padrón M, Barbas JA, Ferrús A: Ariadne-1: a vital Drosophila gene is required in development and defines a new conserved family of ring-finger proteins. Genetics. 2000, 155: 1231-1244.PubMedPubMed Central
- Eisenhaber B, Chumak N, Eisenhaber F, Hauser MT: The ring between ring fingers (RBR) protein family. Genome Biol. 2007, 8: 209-10.1186/gb-2007-8-3-209.PubMedPubMed CentralView Article
- Tan NG, Ardley HC, Scott GB, Rose SA, Markham AF, Robinson PA: Human homologue of ariadne promotes the ubiquitylation of translation initiation factor 4E homologous protein, 4EHP. FEBS Lett. 2003, 554: 501-504. 10.1016/S0014-5793(03)01235-3.PubMedView Article
- Tokunaga F, Sakata S, Saeki Y, Satomi Y, Kirisako T, Kamei K, Nakagawa T, Kato M, Murata S, Yamaoka S, Yamamoto M, Akira S, Takao T, Tanaka K, Iwai K: Involvement of linear polyubiquitylation of NEMO in NF-kappaB activation. Nat Cell Biol. 2009, 11: 123-132. 10.1038/ncb1821.PubMedView Article
- Shimura H, Hattori N, Kubo S, Mizuno Y, Asakawa S, Minoshima S, Shimizu N, Iwai K, Chiba T, Tanaka K, Suzuki T: Familial Parkinson disease gene product, parkin, is a ubiquitin-protein ligase. Nat Genet. 2000, 25: 302-305. 10.1038/77060.PubMedView Article
- Shimura H, Schlossmacher MG, Hattori N, Frosch MP, Trockenbacher A, Schneider R, Mizuno Y, Kosik KS, Selkoe DJ: Ubiquitination of a new form of α-synuclein by Parkin from human brain: implications for Parkinson's Disease. Science. 2001, 293: 263-269. 10.1126/science.1060627.PubMedView Article
- Imai Y, Soda M, Inoue H, Hattori N, Mizuno Y, Takahashi R: An unfolded putative transmembrane polypeptide, which can lead to endoplasmic reticulum stress, is a substrate of Parkin. Cell. 2001, 105: 891-902. 10.1016/S0092-8674(01)00407-X.PubMedView Article
- Zhang Y, Gao J, Chung KK, Huang H, Dawson VL, Dawson TM: Parkin functions as an E2-dependent ubiquitin- protein ligase and promotes the degradation of the synaptic vesicle-associated protein, CDCrel-1. Proc Natl Acad Sci USA. 2000, 97: 13354-13359. 10.1073/pnas.240347797.PubMedPubMed CentralView Article
- Ziviani E, Tao RN, Whitworth AJ: Drosophila parkin requires PINK1 for mitochondrial translocation and ubiquitinates mitofusin. Proc Natl Acad Sci USA. 2010, 107: 5018-5023. 10.1073/pnas.0913485107.PubMedPubMed CentralView Article
- Gegg ME, Cooper JM, Chau KY, Rojo M, Schapira AH, Taanman JW: Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy. Human Mol Genet. 2010, 19: 4861-4870. 10.1093/hmg/ddq419.View Article
- Chuang TH, Ulevitch RJ: Triad3A, an E3 ubiquitin-protein ligase regulating Toll-like receptors. Nat Immunol. 2004, 5: 495-502. 10.1038/ni1066.PubMedView Article
- Nakhaei P, Mesplede T, Solis M, Sun Q, Zhao T, Yang L, Chuang TH, Ware CF, Lin R, Hiscott J: The E3 ubiquitin ligase Triad3A negatively regulates the RIG-1/MAVS signaling pathway by targeting TRAF3 for degradation. PLoS Pathog. 2009, 5: e1000650-10.1371/journal.ppat.1000650.PubMedPubMed CentralView Article
- Ikeda F, Deribe YL, Skanland SS, Stieglitz B, Grabbe C, Franz-Wachtel M, van Wijk SJ, Goswami P, Nagy V, Terzic J, Tokunaga F, Androulidaki A, Nakagawa T, Pasparakis M, Iwai K, Sundberg JP, Schaefer L, Rittinger K, Macek B, Dikic I: SHARPIN forms a linear ubiquitin ligase complex regulating NF-kB activity and apoptosis. Nature. 2011, 471: 637-641. 10.1038/nature09814.PubMedPubMed CentralView Article
- Tokunaga F, Nakagawa T, Nakahara M, Saeki Y, Taniguchi M, Sakata S, Tanaka K, Nakano H, Iwai K: SHARPIN is a component of the NF-kB-activating linear ubiquitin chain assembly complex. Nature. 2011, 471: 633-636. 10.1038/nature09815.PubMedView Article
- Marin I: RBR ubiquitin ligases: Diversification and streamlining in animal lineages. J Mol Evol. 2009, 69: 54-64. 10.1007/s00239-009-9252-3.PubMedView Article
- Khang HY, Yeh S, Fujimoto N, Chang C: Cloning and characterization of human prostate coactivator ARA54, a novel protein that associates with the androgen receptor. J Biol Chem. 1999, 274: 8570-8576. 10.1074/jbc.274.13.8570.View Article
- Urakov VN, Valouev IA, Lewitin EI, Paushkin SV, Kosorukov VS, Kushnirov VV, Smirnov VN, Ter-Avanesyan MD: Itt1p, a novel protein inhibiting translation termination in Saccharomyces cerevisiae. BMC Mol Biol. 2001, 2: 9-10.1186/1471-2199-2-9.PubMedPubMed CentralView Article
- Beasley SA, Hristova VA, Shaw GS: Structure of the Parkin in-between-ring domain provides insights for E3-ligase dysfunction in autosomal recessive Parkinson's disease. Proc Natl Acad Sci USA. 2007, 104: 3095-3100. 10.1073/pnas.0610548104.PubMedPubMed CentralView Article
- Capili AD, Edghill EL, Wu K, Borden KL: Structure of the C-terminal RING finger from a RING-IBR-RING/TRIAD motif reveals a novel zinc-binding domain distinct from a RING. J Mol Biol. 2004, 340: 1117-1129. 10.1016/j.jmb.2004.05.035.PubMedView Article
- Ardley HC, Tan NG, Rose SA, Markham AF, Robinson PA: Features of the parkin/ariadne-like ubiquitin ligase, HHARI, that regulate its interaction with the ubiquitin-conjugating enzyme, Ubch7. J Biol Chem. 2001, 276: 19640-19647. 10.1074/jbc.M011028200.PubMedView Article
- Brzovic PS, Keeffe JR, Nishikawa H, Miyamoto K, Fox D, Fukuda M, Ohta T, Klevit R: Binding and recognition in the assembly of an active BRCA1/BARD1 ubiquitin-ligase complex. Proc Natl Acad Sci USA. 2003, 100: 5646-5651. 10.1073/pnas.0836054100.PubMedPubMed CentralView Article
- Verdecia MA, Joazeiro CA, Wells NJ, Ferrer JL, Bowman ME, Hunter T, Noel JP: Conformational flexibility underlies ubiquitin ligation mediated by the WWP1 HECT domain E3 ligase. Mol Cell. 2003, 11: 249-259. 10.1016/S1097-2765(02)00774-8.PubMedView Article
- Maruyama M, Ikeuchi T, Saito M, Ishikawa A, Yuasa T, Tanaka H, Hayashi S, Wakabayashi K, Takahashi H, Tsuji S: Novel mutations, pseudo-dominant inheritance, and possible familial affects in patients with autosomal recessive juvenile parkinsonism. Ann Neurol. 2000, 48: 245-250. 10.1002/1531-8249(200008)48:2<245::AID-ANA15>3.0.CO;2-2.PubMedView Article
- Wang C, Tan JM, Ho MW, Zaiden N, Wong SH, Chew CL, Eng PW, Lim TM, Dawson TM, Lim KL: Alterations in the solubility and intracellular localization of parkin by several familial Parkinson's disease-linked point mutations. J Neurochem. 2005, 93: 422-431. 10.1111/j.1471-4159.2005.03023.x.PubMedView Article
- Hristova VA, Beasley SA, Rylett RJ, Shaw GS: Identification of a novel Zn2+-binding domain in the autosomal recessive juvenile Parkinson-related E3 ligase parkin. J Biol Chem. 2009, 284: 14978-14986. 10.1074/jbc.M808700200.PubMedPubMed CentralView Article
- Rankin CA, Roy A, Zhang Y, Richter M: Parkin, a top level manager in the cell's sanitation department. Open Biochem J. 2011, 5: 9-26. 10.2174/1874091X01105010009.PubMedPubMed CentralView Article
- Diao J, Zhang Y, Huibregtse JM, Zhou D, Chen J: Crystal structure of SopA, a Salmonella effector protein mimicking a eukaryotic ubiquitin ligase. Nat Struct Mol Biol. 2008, 1: 65-70.View Article
- Salvat C, Wang G, Dastur A, Lyon N, Huibregtse JM: The -4 phenylalanine is required for substrate ubiquitination catalyzed by HECT ubiquitin ligases. J Biol Chem. 2004, 279: 18935-18943. 10.1074/jbc.M312201200.PubMedView Article
- Kirisako T, Kamei K, Murata S, Kato M, Fukumoto H, Kanie M, Sano S, Tokunaga F, Tanaka K, Iwai K: A ubiquitin ligase complex assembles linear polyubiquitin chains. EMBO J. 2006, 25: 4877-4887. 10.1038/sj.emboj.7601360.PubMedPubMed CentralView Article
- Hampe C, Ardila-Osorio H, Fournier M, Brice A, Corti O: Biochemical analysis of Parkinson's disease-causing variants of Parkin, an E3 ubiquitin-protein ligase with monoubiquitylation capacity. Hum Mol Genet. 2006, 15: 2059-2075. 10.1093/hmg/ddl131.PubMedView Article
- Matsuda N, Kitami T, Suzuki T, Mizuno Y, Hattori N, Tanaka K: Diverse effects of pathogenic mutations of Parkin that catalyze multiple monoubiquitylation in vitro. J Biol Chem. 2006, 281: 3204-3209.PubMedView Article
- Olzmann JA, Li L, Chudaev MV, Chen J, Perez FA, Palmiter RD, Chin LS: Parkin-mediated K63-linked polyubiquitination targets misfolded DJ-1 to aggresomes via binding to HDAC6. J Cell Biol. 2007, 178: 1025-1038. 10.1083/jcb.200611128.PubMedPubMed CentralView Article
- Doss-Pepe EW, Chen L, Madura K: Alpha-synuclein and parkin contribute to the assembly of ubiquitin lysine 63-linked multiubiquitin chains. J Biol Chem. 2005, 280: 16619-16624. 10.1074/jbc.M413591200.PubMedView Article
- Chaugule VK, Burchell L, Barber KR, Sidhu A, Leslie SJ, Shaw GS, Walden H: Autoregulation of Parkin activity through its ubiquitin-like domain. EMBO J. 2011, 30: 2853-2867. 10.1038/emboj.2011.204.PubMedPubMed CentralView Article
- Staropoli JF, McDermott C, Martinat C, Schulman B, Demireva E, Abeliovich A: Parkin is a component of an SCF-like ubiquitin ligase complex and protects postmitotic neurons from kainate excitotoxicity. Neuron. 2003, 37: 735-749. 10.1016/S0896-6273(03)00084-9.PubMedView Article
- Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22: 4673-4680. 10.1093/nar/22.22.4673.PubMedPubMed CentralView Article
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