Involvement of Plasmodium falciparum protein kinase CK2 in the chromatin assembly pathway
- Eeshita G Dastidar†1, 2,
- Guillem Dayer†3,
- Zoe M Holland†4,
- Dominique Dorin-Semblat3, 4,
- Aurélie Claes1, 2,
- Arnaud Chêne1, 2,
- Amit Sharma5,
- Romain Hamelin6,
- Marc Moniatte6,
- Jose-Juan Lopez-Rubio1, 2,
- Artur Scherf1, 2Email author and
- Christian Doerig3, 4, 7Email author
© Dastidar et al; licensee BioMed Central Ltd. 2012
Received: 27 December 2011
Accepted: 31 January 2012
Published: 31 January 2012
Protein kinase CK2 is a pleiotropic serine/threonine protein kinase with hundreds of reported substrates, and plays an important role in a number of cellular processes. The cellular functions of Plasmodium falciparum CK2 (PfCK2) are unknown. The parasite's genome encodes one catalytic subunit, PfCK2α, which we have previously shown to be essential for completion of the asexual erythrocytic cycle, and two putative regulatory subunits, PfCK2β1 and PfCK2β2.
We now show that the genes encoding both regulatory PfCK2 subunits (PfCK2β1 and PfCK2β2) cannot be disrupted. Using immunofluorescence and electron microscopy, we examined the intra-erythrocytic stages of transgenic parasite lines expressing hemagglutinin (HA)-tagged catalytic and regulatory subunits (HA-CK2α, HA-PfCK2β1 or HA-PfCK2β2), and localized all three subunits to both cytoplasmic and nuclear compartments of the parasite. The same transgenic parasite lines were used to purify PfCK2β1- and PfCK2β2-containing complexes, which were analyzed by mass spectrometry. The recovered proteins were unevenly distributed between various pathways, with a large proportion of components of the chromatin assembly pathway being present in both PfCK2β1 and PfCK2β2 precipitates, implicating PfCK2 in chromatin dynamics. We also found that chromatin-related substrates such as nucleosome assembly proteins (Naps), histones, and two members of the Alba family are phosphorylated by PfCK2α in vitro.
Our reverse-genetics data show that each of the two regulatory PfCK2 subunits is required for completion of the asexual erythrocytic cycle. Our interactome study points to an implication of PfCK2 in many cellular pathways, with chromatin dynamics being identified as a major process regulated by PfCK2. This study paves the way for a kinome-wide interactomics-based approach to elucidate protein kinase function in malaria parasites.
Malaria, a disease caused by parasitic protozoa of the genus Plasmodium (phylum Apicomplexa), is responsible for the death of one million people every year, mostly children living in sub-Saharan Africa . The burden of morbidity and mortality inflicted by this disease presents a serious hindrance to socioeconomic development, causing up to a 1.3% decrease in the gross domestic product of countries with a high malaria rate . The number of deaths due to malaria doubled in the last two decades of the 20th century, with one of the primary reasons being the spread of parasite resistance to anti-malarial drugs, especially chloroquine . Artemisinin combination therapy (ACT) has helped to reverse this trend, but the worrying appearance in southeast Asia of parasite isolates with reduced artemisinin sensitivity suggests that this may be a short-lived respite [4, 5]. The development of anti-malarial drugs with novel modes of action remains an urgent priority.
Reversible phosphorylation is an integral part of many cellular processes, and perturbations in its regulation have been identified in many diseases, such as cancer, diabetes and rheumatoid arthritis [6, 7]. Protein kinases (PKs) are therefore attracting much interest as potential drug targets, and recent successes in cancer chemotherapy based on kinase inhibitors indicate that these molecules can perform as drugs with appropriate selectivity, potency and pharmacokinetic properties . Investigations of the druggability of the genome identified serine/threonine PKs as one of the most promising groups for future small molecule inhibitors, comprising up to 20% of the druggable genome .
Protein kinase CK2 (thereafter called CK2) is a pleiotropic serine/threonine PK, with over 300 substrates identified in mammalian cells  and documented roles in multiple cellular processes, including differentiation, proliferation, survival, translation, apoptosis, transformation, tumorigenesis, RNA synthesis, cell cycle progression, cell morphology and polarity, cellular responses to stress and to DNA damage, and circadian rhythm [11, 12]. The enzyme phosphorylates a range of DNA-binding proteins, nuclear oncoproteins, and transcription factors , and several studies have highlighted the predominance of CK2 substrates involved in transcription, chromatin structure, and gene expression [10, 12, 14]. Taken together, these studies highlight transcriptional regulation as one of the major roles of the enzyme . Controlled cellular compartmentalization is crucial for the function of a pleiotropic enzyme such as CK2, and substrate selection is presumably dependent on proper targeting . Although predominantly nuclear , mammalian CK2 has also been found in the cytoplasm, in association with a number of organelles such as the Golgi apparatus, endoplasmic reticulum, and ribosomes , and plant chloroplasts . The human genome contains two genes encoding catalytic (α and α') subunits, and a single gene encoding a regulatory (β) subunit. CK2 is present in human cells as a tetrameric complex comprising two identical β subunits and two catalytic subunits, which can be assembled in a α/α, α/α' or α'/α' configuration. CK2α has been found to be essential for viability in all organisms in which its role has been assessed, including P. falciparum . The requirement for viability of the β subunits of CK2 varies across species: disruption of the single metazoan β subunit is lethal in mice  and Caenorhabditis elegans , whereas disruption of either or both of the β subunits in Saccharomyces cerevisiae is not lethal [14, 22, 23].
Phylogenetic analysis of P. falciparum PKs [24, 25] identified a CK2α ortholog and two putative CK2β subunits, PfCK2β1 and PfCK2β2 (see Holland et al.  for alignments with CK2 subunits form other organisms). The stoichiometry of the PfCK2 complexes is not known; by analogy with other systems, it is hypothesized that a homo- or heterodimer of β subunits is complexed with two catalytic subunits in the holoenzyme, but this still requires experimental demonstration. Using transcriptomics, it has been shown that all three subunits are expressed throughout the asexual erythrocytic asexual cycle, in gametocytes and in sporozoites [26, 27]. Proteomics data available for PfCK2α and PfCK2β1 indicate that both proteins are present in the asexual erythrocytic stages and gametocytes, and that the α subunit is also present in sporozoites . We showed previously that PfCK2α is essential for completion of the erythrocytic asexual cycle , but neither the requirement of the β subunits for viability nor the cellular processes implicating the enzyme have been assessed.
In the current study, we used a reverse-genetics approach, and found that both regulatory subunits are also essential for intra-erythrocytic asexual proliferation. Reasoning that identifying interactors of the regulatory subunits might provide useful insight into the cellular functions of the PfCK2 holoenzyme, we analyzed the protein composition of complexes obtained by co-immunoprecipitation of PfCK2β1 or PfCK2β2 from transgenic parasite lines expressing tagged subunits. This allowed us to recover proteins involved in many cellular processes, consistent with our immunofluorescence assay (IFA) data localizing the kinase subunits to both nuclear and cytoplasmic compartments. To investigate the role of CK2 in gene regulation specifically, we focused validation experiments on nuclear proteins, and found that several proteins involved in chromatin dynamics, which were recovered in the β subunit pull-downs, are substrates for PfCK2α in vitro. Taken together, our data identify PfCK2 as an essential kinase involved in chromatin dynamics.
Both of the CK2β subunits play crucial roles in the erythrocytic asexual cycle
To ascertain whether pfckβ1 can be disrupted if the subunit is provided through expression of an episomal copy of the gene, we constructed a complementation plasmid (pCHD-PfCK2β1) containing the full-length pfckβ1 gene under the control of the PfHsp86 promoter and a 3' UTR. In parallel with the transfection of the pCAM-BSD-KOPfCK2β1 plasmid alone, a population of parasites was transfected with both pCAM-BSD-KOPfCK2β1 and pCHD-PfCK2β1. PCR (Figure 1B) and Southern blot (Figure 1C) analyses showed that disruption of the targeted locus occurred only in this doubly transfected, doubly resistant parasite culture. Probing the membrane with a BSD probe (Figure 1C, left panel) confirmed that integration had occurred only in the parasites transfected with both the knockout and complementation plasmids. The pfckβ1 probe hybridized to the 11.1 kb band, corresponding to the wild-type locus, in the lane that contained DNA from untransfected parasites and from parasites transfected with the knockout plasmid alone (lanes 1 to 3). This band was undetectable in DNA from parasites transfected with both the knockout and complementation plasmid (lane 4), indicating that the gene can be disrupted only when an additional cassette coding for the PfCK2β1 subunit is provided to the parasites. There are multiple possibilities for recombination of the knockout and complementation plasmids before or after integration, which presumably accounts for the additional bands. The crucial observation is the disappearance of the wild-type-specific band only in the doubly transfected parasite culture. Similar results were obtained for PfCK2β2 (see Additional file 2, Figure A1), indicating that both regulatory subunits play crucial roles in parasite proliferation in the red blood cell.
PfCK2 subunits localize to both cytoplasmic and nuclear compartments
Purification and mass spectrometry analysis of protein complexes involving the PfCK2β subunits
To gain insight into the functions of PfCK2 through identification of the proteins with which the holoenzyme interacts, we subjected protein extracts from the parasite line expressing HA-tagged PfCK2β1 to immunoprecipitation using the anti-HA antibody; the same analysis was performed on extracts from wild-type parasites as a negative control. The immunoprecipitates were separated by SDS-PAGE, and a western blotting was performed using anti-PfCK2β1 to verify the immunoprecipitation efficiency (see Additional file 2, Figure A4A). A parallel gel was loaded and slices excised (see Additional file 2, Figure A4B) for mass spectrometry analysis.
Potential interactors (see Additional file 3, Table A2) were identified based on the number of unique peptides recovered, and on the unweighted spectrum count of the number of detected peptides. We considered proteins as potential hits if they were present in the immunoprecipitate from the HA-tagged line but were absent (or present in trace amounts only) in those from the wild-type parasites. The same experiment was performed in parallel with the parasite line expressing HA-tagged PfCK2β2 (see Additional file 4, Table A3, for the list of recovered proteins), although in this case we were unable to perform western-blot verification of the immunoprecipitate because of the poor quality of the anti- PfCK2β2 antibody.
PfCK2α was pulled down with both HA-PfCK2β1 and HA-PfCK2β2, in line with the previously reported in vitro interaction between the catalytic subunit and both regulatory subunits . Interestingly, PfCK2β1 and PfCK2β2 were present in similar amounts in both immunoprecipitates, suggesting that complexes containing both regulatory subunits may be relatively abundant.
A different distribution pattern was seen when the same protocol, using the same anti-HA antibody, was applied to parasites expressing other HA-tagged kinases, such as PfCK1 (data not shown); this result lends support to the specificity of the pull-down approach. For both PfCK2β subunits, the highest numbers of potential interactors were found in the processes of 1) post-translational modifications, and 2) mitosis and chromosome separation. As we were aiming to understand the nuclear function of PfCK2, we focused further investigations on the latter process.
Potential interactors in the nucleosome assembly and regulation pathway.a
Histone H2A variant
Histone H2B variant
High mobility group protein
Chromodomain helicase DNA-binding protein
Structure-specific recognition protein
P. falciparum Naps, histones and Alba proteins are substrates for PfCK2α in vitro
We next examined the ability of histones to act as substrates for recombinant GST-PfCK2α. Recently, several phosphorylation sites in plasmodial histones have been identified (; Dastidar et al., unpublished data), and because several histones were recovered in the PfCK2β precipitates (Table 1), we thought that PfCK2α might be one of the kinases that can phosphorylate histones. When purified parasite native histones were subjected to a GST-PfCK2α kinase assay, preferential phosphorylation of histone H2B (13 kDa) was seen, whereas the dead-mutant kinase failed to phosphorylate any of the histones (Figure 5B). We also tested in PfCK2α kinase assays all four P. falciparum Alba members expressed as GST fusion proteins , as well as the nuclear protein PfSir2, which is implicated in establishment of heterochromatin in sub-telomeric region and hence represses var gene expression [40–42]. PfAlba3, PfAlba4, and Sir2 were not phosphorylated by GST-PfCK2α (Figure 5E). By contrast, PfAlba1 (55 kDa) and PfAlba2 (52 kDa) gave signals on the autoradiographs after a kinase assay was performed with GST-PfCK2α, whereas none of the substrates subjected to the GST-PfCK2α dead mutant was phosphorylated (Figure 5C).
Our reverse-genetics data strongly suggest that both PfCK2β subunits are essential for parasite viability, indicating that they have non-redundant functions in the parasite. This was unexpected given the dispensability of the β subunits for viability in S. cerevisiae (which has two CK2β subunits that can be knocked out individually or simultaneously [14, 22, 23]) and ib Schizosaccharomyces pombe (which had a single β subunit) . However, the single β subunit present in C. elegans  and in mice  is essential. The different interactomes for the CK2β subunits in S. cerevisiae  are consistent with functional specialization of each β subunit. The immunoprecipitates obtained with PfCK2β1 and PfCK2β2, despite displaying significant overlap in the identity of recovered proteins, also contained hits that were found in only one of the two immunoprecipitates (see Additional file 5, Table A4). This would be consistent with the non-redundancy in function suggested by reverse genetics; however, we do not have sufficient data to draw any firm conclusion in this respect. The dataset presented in the supplementary material (see Additional file 3, Table A2; see Additional file 4, Table A3) is from one of three experiments. Although several hits obtained in the first dataset were confirmed in two further experiments, fewer proteins were recovered in the latter, pre-empting statistical analysis, More work is needed to confirm that individual proteins are truly absent from one of the other PfCK2β interactome. However, the data (see Additional file 3, Table A2; see Additional file 4, Table A3) nevertheless enabled us to propose assignment of specific functions to PfCK2.
Using recombinant proteins in pull-down experiments, we previously found that PfCK2α is able to interact with both β subunits in vitro . MS analysis of immunoprecipitated subunits (this study) indicates that these interactions are also found in parasite extracts. CK2 is known to have several functions in various organisms [11, 12], and the glimpse into the interactomes of the P. falciparum CK2 regulatory subunits provided by our study is consistent with their involvement in a number of cellular processes. Implementing the same protocol with different HA-tagged kinases such as PfCK1 (data not shown) yielded protein repertoires that were clearly distinct from those described here for the PfCK2β subunits, lending support to the specificity of the approach. Potential PfCK2 interactors were found in 17 out of 18 metabolic processes described on the MPMP website. Many processes displayed only a very small number of hits; whether or not these represent genuine interactors will require in-depth investigations of each individual case.
By contrast, other processes and pathways exhibited a large proportion of their proteins recovered in the immunoprecipitates, suggesting that a number of distinct functional complexes had been pulled down. A striking example is the 'nucleosome assembly and regulation' pathway, many components of which were found in both PfCK2β1 and PfCK2β2 immunoprecipitates (Table 1). This included both PfNAPs, many histones, and several proteins involved in chromatin remodelling (ISWI, CHD1, high mobility group protein, and structure-specific recognition protein). Interestingly, all recovered proteins were members of the so-called 'activating factors' in this pathway, whereas none of the 'inactivating factors' were present in the immunoprecipitates (see Additional file 2, Figure A5), which again is consistent with the specificity of the approach. Some of these interactions were validated by coupled co-immunoprecipitation/western-blot analysis (see Additional file 2, Figure A6) and in vitro kinase assays (Figure 5).
We found that PfCK2α preferentially phosphorylated histone H2B in vitro in a mixture of native histones extracted from the parasite. Histone post-translational modifications such as methylation and acetylation play key roles in the control of expression of virulence genes (for example, the var family) in malaria parasites ) Similarly, histone phosphorylation is known to play an important regulatory role in other eukaryotes , and in yeast, CK2 phosphorylates histone H4 at serine 1 as part of a DNA damage-signaling pathway . Although previous studies have identified few phosphorylation marks in P. falciparum histones, we recently characterized several novel phosphorylation marks on the parasite's histones (Dastidar et al., unpublished). Whether PfCK2 is responsible for phosphorylation of any of these sites in vivo, and the functional consequences of these phosphorylation events, remain to be investigated. Nevertheless, taken together, our data strongly suggest that PfCK2-dependent phosphorylation may play a role in chromatin dynamics.
We also tested other known nuclear proteins with a potential role in gene regulation to see if they could be substrates for the kinase. Of all the proteins tested, recombinant PfAlba1 and PfAlba2 were found to be substrates for the PfCK2α in vitro (Figure 5), and PfAlba1 was found to be phosphorylated in parasite extracts on several residues [46, 47], including one in an acidic stretch consistent with CK2 substrate preferences (Table 2). By contrast, recombinant PfAlba3, PfAlba4, and PfSir2 were not phosphorylated by the kinase (Figure 5E). PfAlba1 and PfAlba2 belong to the same phylogenetic cluster, which is distinct from that containing PfAlba3 and PfAlba4 ([51, 52]), consistent with their different ease if phosphorylation by PfCK2. Moreover, the former two PfAlbas possess an arginine- and glycine-rich RGG domain in addition to the Alba domain. It has been shown in S. cerevisiae that phosphorylation of the RGG domains affects protein localization and transport between the nucleus and cytoplasm . Given that PfAlba1 and PfAlba2 concentrate at the perinuclear space in ring-stage parasites, but expand to the cytoplasm of trophozoites and schizonts , it is possible that phosphorylation by PfCK2 may contribute to the regulation of their nucleocytoplasmic transport.
Several proteins involved in mitosis and chromosome separation and in DNA replication and DNA damage pathways (for example, several replication factor C subunits, ATP-dependent DNA helicase, DNA ligase 1) were also pulled down. Implication of CK2 in the DNA damage response is well documented in other systems , and a DNA repair pathway has been characterized in P. falciparum , of which the DNA polymerase and DNA ligase components can be retrieved in the CK2β immunoprecipitates (see Additional file 3, Table A2; see Additional file 4, Table A3; see Additional file 5, Table A4). Because DNA repair is markedly reduced in the highly drug-resistant W2 isolate, which displays a higher rate of drug-resistance acquisition than do drug-sensitive strains , it might be of interest to compare CK2 activity in the W2 versus drug-sensitive clones.
Although many of the proteins identified in the immunoprecipitates were of nuclear origin, a number of potential interactors were associated with other cellular compartments. Some of the recovered proteins were putative or established Maurer's cleft proteins (for example, Maurer's cleft 2 transmembrane protein; Pfmc-2TM), proteins involved in invasion and motility processes (for example, glutamate-riche protein, merozoite surface protein 2 precursor, RhopH3, and other rhoptry-associated proteins) or in cell-cell interaction processes (for example, mature parasite-infected erythrocyte surface antigen, PfEMP2 and rifins). Likewise, several proteins and enzymes involved in redox metabolism were recovered (thioredoxin, thioredoxin-related protein, thioredoxin peroxidase, glutathione peroxidase, glutathione S-transferase, ribonucleotide reductase). This diversity is consistent with our IFA, western blot, and immuno-EM data localizing all the three subunits to both the nuclear and cytoplasmic compartments. Although these results require confirmation by other approaches, and despite the fact that we cannot exclude false positives in our interactome list, they are consistent with an involvement of PfCK2 kinase in varied cellular functions in the parasite.
The availability of parasite lines expressing individual tagged PKs for the almost entire P. falciparum kinome  now allows implementation of a kinome-wide investigation of kinase function based on the interactomics approach used here for PfCK2. Preliminary data obtained with other PKs suggest that distinct functions can therefore be assigned to different enzymes on the basis of specifically pulled-down protein repertoires.
In conclusion, we have identified the PfCK2 complex as a kinase distributed to different cellular compartments. Our data point to PfCK2 as a pleiotropic kinase with various cellular functions. In this study, we explored potential nuclear functions of the kinase, and we propose a role for PfCK2 in modifying a number of chromatin-related proteins, several of which are also substrates for the catalytic subunit of PfCK2 in vitro. Furthermore, reverse genetics data show that all three subunits of the kinase are essential for the viability of the blood-stage parasite, confirming its potential as target for chemotherapeutic intervention. This study paves the way for a kinome-wide interactomics-based approach to elucidate PK function in malaria parasites.
Construction of the knockout plasmids pCAM-BSD-KOPfCK2β1 and pCAM-BSD-KOPfCK2β2
Fragments from central region of the PfCK2β1 and PfCK2β2 ORFs were amplified by PCR and inserted between the BamHI and NotI sites of the pCAM-BSD plasmid , which contains the Aspergillus terreus blasticidin-S-deaminase gene, whose gene product confers resistance to blasticidin (see Additional file 1, table A1, for cloning primers).
Construction of the complementation plasmids pCHD-PfCK2β1 and pCHD-PfCK2β2
A plasmid for in vivo episomal expression of PfCK2β1 and PfCK2β2 subunits were constructed as follows. The full-length coding sequences were first inserted between the BglII and NotI sites of plasmid pHGB , and then transferred into plasmid pCHD-1/2  by a clonase reaction (Gateway system, Invitrogen) according to the manufacturer's instructions. Plasmid pCHD-1/2 includes a cassette encoding human dihydrofolate reductase, conferring resistance to the antifolate drug WR99210. Parasites that were transfected with both a KO plasmid and a complementation plasmid were cultured under double-drug selection (see below).
3'-tagging of plasmids
The 3' end of the PfCK2β2 coding sequence (573 bp, omitting the stop codon) was amplified by PCR using primers incorporating PstI and BamHI restriction sites and inserted between the same sites of the pCAM-BSD-HA plasmid . For pfCK2β1, the 3' end of the gene (540 bp) was amplified, then the PCR product was treated with the restriction enzymes PstI and BglII and inserted between the PstI and BamHI sites of pCAM-BSD-HA.
Parasite culture and transfection
Four P. falciparum strains (3D7A  and three HA-tagged strains of PfCK2 subunits: PfCK2αHA, PfCK2β1HA and PfCK2β2HA)  were grown in human erythrocytes as described previously . The medium for the HA-tagged lines was supplemented with blasticidin (2.5 μg/ml; VWR International Ltd., West Chester, PA, USA). Cultures of the P. falciparum clone 3D7A were maintained at 37°C in RPMI 1640 medium (Gibco) supplemented with 25 mmol/l sodium bicarbonate, 2 mmol/l L-glutamine, 300 mmol/l hypoxanthine, 10 μg/ml gentamicin, and lipid-rich bovine serum albumin (BSA) (AlbuMAX II; Sigma-Aldrich, St Louis, MO, USA). Cultures were seeded at 5% hematocrit volume and maintained at a parasitemia of 1 to 10% with daily changes of medium. The incubator was flushed with a gas mixture containing 5% CO2. For transfection, asexual blood-stage parasites were synchronized by sorbitol treatment  so as to result in the majority being ring-stage parasites. Forty-eight hours later, the ring-stage parasites were transfected by electroporation with 100 μg of purified plasmid DNA in buffer (Cytomix; Gibco-BRL, Grand Island, New York, NY, USA) as described previously . Blasticidin (2.5 μg/ml) was added to the culture medium to select for transformed parasites. Parasites under double selection had 5 nmol/l WR99210 added to the culture medium in addition to blasticidin. Parasites were maintained in this supplemented medium from 2 days post-transfection.
DNA extraction and Southern blotting
Parasite cultures were lysed in 0.15% saponin. The parasite pellets were resuspended in cold phosphate-buffered saline, and treated with 150 μg/ml proteinase K and 2% SDS at 55°C for 2 to 3 hours. Total DNA was extracted in phenol:chloroform:isoamyl alcohol (25:24:1) and precipitated in ethanol and 0.3 mol/l sodium acetate. DNA was digested with ClaI and EcoRI for PfCK2β1, and with ClaI and NcoI for PfCK2β2.
Cytoplasmic and nuclear extracts were prepared as described previously [41, 62]. Using standard procedures. SDS-PAGE was performed and DNA was transferred to nitrocellulose membranes, which were probed using either commercially available antibodies against HA-tag (ab9110; 1:1000 dilution), histone H3 (ab1791; 1:2500 dilution), P. falciparum aldolase (ab38905; 1:1000) (all Abcam, Cambridge, MA, USA) or subunit-specific antibodies generated in rabbits against the PfCK2β1-derived peptide DSNKDLQDSKSDKS (1:500 dilution) or the PfCK2β2-derived peptide DEINRDSEEMYKNK (1:1000 dilution (both BioGenes GmBH, Berlin, Germany) After probing with appropriate secondary antibodies conjugated to horseradish peroxidase (GE Healthcare Life Sciences, Princeton, NJ, USA), the membranes were developed with a commercial substrate (Super Signal West Pico Chemiluminescent Substrate; Thermo Fisher Scientific Inc., Rockford, IL, USA) in accordance with the manufacturer's recommendations.
IFAs were performed on synchronized stages (ring, trophozoite, and schizont) of the three HA-tagged strains of the PfCK2 subunits as described previously . The fixed cells were incubated with primary antibodies anti-HA (ab9110; 1:2000 dilution) and anti-Exp2  (1:800 dilution) (both Abcam). After incubating with appropriate secondary antibodies, the cells were examined under a microscope (Nikon Instruments, Tokyo, Japan).
Immuno-gold electron microscopy
Infected erythrocytes were fixed in 1% glutaraldehyde in RPMI-HEPES buffer for 1 hour at 4°C. After washing, polymerization in agar (Type IX; Sigma-Aldrich), and dehydration with ethanol, the samples were transferred into embedding resin (LR-White; London Resin Company Ltd, Reading, Berkshire, UK), and polymerized for 12 hours at 4°C, and cut. Ultrathin sections of the sample were collected and mounted on Cu/Pd grids. Free aldehyde groups were blocked by incubating the samples for 5 minutes in 50 mmol/l NH4Cl. Sections were blocked in phosphate-buffered saline containing 5% (v/v) normal goat serum, 1% (w/v) BSA and 0.01% (v/v) Tween-80. The washed grids were incubated for 2 hours in antiserum diluted in phosphate-buffered saline containing 1% (v/v) normal goat serum, 1% (w/v) BSA and 0.01% (v/v) Tween-80 and anti-HA antibody (ab9110; Abcam). Samples were washed and incubated for 45 minutes with 12-nm gold-labeled goat anti-rabbit IgG (111-205-144; Jackson Immunoresearch Laboratories, Inc., West Grove, PA, USA). Washed sections were stained for 15 minutes with aqueous 4% uranyl acetate followed by staining for 2 minutes with 1% lead citrate. Sections were analyzed using an electron microscope (JEM-1200EX; JEOL Ltd, Tokyo, Japan) at 120 kV.
Parasite proteins extraction and immunoprecipitation
Parasite cultures were lysed in 0.15% saponin and pellets were stored at -80°C. Proteins were extracted on ice using mammalian protein extraction reagent (M-PER) (Profound™ Mammalian HA-tag IP/Co-IP application; Thermo Fisher Scientific) with protease inhibitors (1:25; Complete Protease Inhibitor Cocktail; Roche Diagnostics, Basel, Switzerland) and benzonase nuclease (1:500; Novagen, Darmstadt, Germany). The lysates were cleared by centrifugation at 11,180 g for 15 minutes at 4°C, and the concentration of proteins measured by the Bradford assay. Proteins were loaded on a spin column with a pre-inserted frit, preventing bead loss between washes, and 6 μl (50% v/v_ of anti-HA crosslinked onto sepharose beads were added (both included in the kit used for protein extraction; Thermo Fisher Scientific). The columns were then transferred to a rocker platform and incubated overnight. The flow-through was collected by centrifugation at 300 g for 10 seconds at room temperature. The columns were washed three times using the Tris-buffered saline (TBS) provided in the kit, followed by a short burst of centrifugation (300 g for 10 seconds). Finally, any proteins bound to the beads were eluted using boiled 4× Laemmli buffer, and recovered by centrifugation (15,600 g for 3 minutes). Aliquots of protein extracts and flow-through liquid were taken before and after immunoprecipitation to monitor the yield.
Mass spectrometry analysis
Samples were separated by SDS-PAGE on 10% polyacrylamide gel, which was then stained with Coomassie blue (Biosafe; Bio-Rad Laboratories, Inc., Hercules, CA, USA), and later placed in 10% ethanol and 1% acetic acid. Each lane was removed from the gel and cut into eight slices, then the proteins were digested in the gel using trypsin As follows. Briefly, the samples were reduced in 10 mmol/l dithioerythritol (DTE) and alkylated in 55 mmol/l iodoacetamide (IAA), the gel pieces were dried. The samples were incubated with 12.5 ng/ml trypsin overnight at 37°C. The tryptic peptides were extracted from the gel slices, dried, and resuspended in 2% acetonitrile (ACN):0.1% formic acid (FA) for liquid chromatography/tandem mass spectrometry (LC-MS-MS) analysis
A mass spectrometer (LTQ Orbitrap XL (Thermo Fischer Scientific) equipped with an ultraperformance LC (UPLC) system (NanoAcquity; Waters Ltd, Elstree, Hertfordhshire, UK) was used. Peptides were trapped in a custom-made precolumn (Magic C18 AQ stationary phase, 5 μm diameter, 200Å pore, 0.1 × 20 mm, Michrom Bioresource) and separated in a custom-made main column (Magic C18 AQ, 3 μm diameter, 100Å pore, 0.75 × 150 mm), using a run of 53 minutes and a gradient of H2O:ACN:FA 98%:2%:0.1% (solvent A) and ACN:H2O:FA 98%:1.9%:0.1% (solvent B). The gradient was run at a flow rate of 250 nl/min as follows: 100% A for 3 min, 30% B within 36 min, 47% B within 14 min, 90% B within 5 min held for 5 min and 100% A for 17 min. The MS/MS was operated in an information-dependent mode, in which each full MS analysis was followed by 10 MS/MS acquisitions, during which the most abundant peptide were selected for collision-induced dissociation (CID) to generate tandem mass spectra. The normalized collision energies were set to 35% for CID.
Data search was performed using Mascot software (version 2.3; Matrix Science Inc., Boston, MA, USA) and Proteome Discoverer (v.1.1; Thermo Fisher Scientific), and the sequences searched against a concatenated database consisting of non-redundant Plasmodium database (PlasmoDB, version 6.4; http://plasmodb.org) and the reversed-sequence version of the same database and common contaminant proteins. Finally, results were imported into Scaffold (version 3_00_8 Proteome Software; http://proteomesoftware.com/) for validation of protein identification, normalization, and comparison of spectral count. Peptide identifications were accepted if they could be established at a probability of greater than 95% as determined by the ProteinProphet algorithm . Protein identifications were accepted if they were assigned at least two unique, validated peptides, and could be established with at least 99% probability as determined by the ProteinProphet algorithm .
The GST-PfCK2α and GST-PfCK2α-dead K72M mutant fusion proteins  were purified from E. coli (BL21 Gold strain) as described previously . PfCK2α kinase activity was determined by measuring 33P or 32P incorporation using γ[33P]-ATP or γ[32P]-ATP. All the substrates (Alba1, Alba2, Alba3, Alba4, PfSir2; kindly provided by C. Scheidig-Benatar), and extracted P. falciparum histones were treated with active PfCK2α and dead mutant as described previously . Samples were run on a 16% gel, which was dried and exposed to film for autoradiography.
This research received funding from Inserm, EPFL and the European Community's Seventh Framework Programme (FP7/2007-2013) (MALSIG project to CD and ASh and EviMalar network of Excellence to CD and ASc), by Inserm, EPFL, the FP6 (SIGMAL and ANTIMAL projects to CD and BioMalPar network of Excellence to ASc), and by a European Research Council Executive Agency Advanced Grant (PlasmoEscape 250320) to ASs. EGD benefits from a PhD fellowship funded by the European Union Framework Program 7 Marie Curie Initial Training Network 'Intervention strategies against malaria (InterMalTraining)', contract number 215281. ZMH benefitted from a PhD Studentship form the Wellcome Trust. A postdoctoral fellowship from the Conseil Régional d'Île-de-France (DIM Malinf) to AC is gratefully acknowledged.
- Snow RW, Guerra CA, Noor AM, Myint HY, Hay SI: The global distribution of clinical episodes of Plasmodium falciparum malaria. Nature. 2005, 434 (7030): 214-217. 10.1038/nature03342.PubMedPubMed CentralView ArticleGoogle Scholar
- Sachs J, Malaney P: The economic and social burden of malaria. Nature. 2002, 415 (6872): 680-685. 10.1038/415680a.PubMedView ArticleGoogle Scholar
- Snow RW, Trape JF, Marsh K: The past, present and future of childhood malaria mortality in Africa. Trends Parasitol. 2001, 17 (12): 593-597. 10.1016/S1471-4922(01)02031-1.PubMedView ArticleGoogle Scholar
- Dondorp AM, Nosten F, Yi P, Das D, Phyo AP, Tarning J, Lwin KM, Ariey F, Hanpithakpong W, Lee SJ, et al: Artemisinin resistance in Plasmodium falciparum malaria. N Engl J Med. 2009, 361 (5): 455-467. 10.1056/NEJMoa0808859.PubMedPubMed CentralView ArticleGoogle Scholar
- Dondorp AM, Yeung S, White L, Nguon C, Day NP, Socheat D, von Seidlein L: Artemisinin resistance: current status and scenarios for containment. Nat Rev Microbiol. 2010, 8 (4): 272-280.PubMedGoogle Scholar
- Cohen P: The role of protein phosphorylation in human health and disease. The Sir Hans Krebs Medal Lecture. Eur J Biochem. 2001, 268 (19): 5001-5010. 10.1046/j.0014-2956.2001.02473.x.PubMedView ArticleGoogle Scholar
- Feng Q, Wang H, Ng HH, Erdjument-Bromage H, Tempst P, Struhl K, Zhang Y: Methylation of H3-lysine 79 is mediated by a new family of HMTases without a SET domain. Curr Biol. 2002, 12 (12): 1052-1058. 10.1016/S0960-9822(02)00901-6.PubMedView ArticleGoogle Scholar
- Giamas G, Stebbing J, Vorgias CE, Knippschild U: Protein kinases as targets for cancer treatment. Pharmacogenomics. 2007, 8 (8): 1005-1016. 10.2217/146224188.8.131.525.PubMedView ArticleGoogle Scholar
- Hopkins AL, Groom CR: The druggable genome. Nat Rev Drug Discov. 2002, 1 (9): 727-730. 10.1038/nrd892.PubMedView ArticleGoogle Scholar
- Meggio F, Pinna LA: One-thousand-and-one substrates of PK CK2?. Faseb J. 2003, 17 (3): 349-368. 10.1096/fj.02-0473rev.PubMedView ArticleGoogle Scholar
- Bibby AC, Litchfield DW: The multiple personalities of the regulatory subunit of protein kinase CK2: CK2 dependent and CK2 Independent roles reveal a secret identity for CK2beta. Int J Biol Sci. 2005, 1 (2): 67-79.PubMedPubMed CentralView ArticleGoogle Scholar
- Filhol O, Cochet C: Protein kinase CK2 in health and disease: cellular functions of PK CK2: a dynamic affair. Cell Mol Life Sci. 2009, 66 (11-12): 1830-1839. 10.1007/s00018-009-9151-1.PubMedView ArticleGoogle Scholar
- Olsten ME, Litchfield DW: Order or chaos? An evaluation of the regulation of PK CK2. Biochem Cell Biol. 2004, 82 (6): 681-693. 10.1139/o04-116.PubMedView ArticleGoogle Scholar
- Ackermann K, Waxmann A, Glover CV, Pyerin W: Genes targeted by PK CK2: a genome-wide expression array analysis in yeast. Mol Cell Biochem. 2001, 227 (1-2): 59-66.PubMedView ArticleGoogle Scholar
- Gietz RD, Graham KC, Litchfield DW: Interactions between the subunits of casein kinase II. J Biol Chem. 1995, 270 (22): 13017-13021. 10.1074/jbc.270.22.13017.PubMedView ArticleGoogle Scholar
- Krek W, Maridor G, Nigg EA: Casein kinase II is a predominantly nuclear enzyme. J Cell Biol. 1992, 116 (1): 43-55. 10.1083/jcb.116.1.43.PubMedView ArticleGoogle Scholar
- Faust M, Jung M, Gunther J, Zimmermann R, Montenarh M: Localization of individual subunits of PK CK2 to the endoplasmic reticulum and to the Golgi apparatus. Mol Cell Biochem. 2001, 227 (1-2): 73-80.PubMedView ArticleGoogle Scholar
- Salinas P, Fuentes D, Vidal E, Jordana X, Echeverria M, Holuigue L: An extensive survey of CK2 alpha and beta subunits in Arabidopsis: multiple isoforms exhibit differential subcellular localization. Plant Cell Physiol. 2006, 47 (9): 1295-1308. 10.1093/pcp/pcj100.PubMedView ArticleGoogle Scholar
- Holland Z, Prudent R, Reiser JB, Cochet C, Doerig C: Functional analysis of PK CK2 of the human malaria parasite Plasmodium falciparum. Eukaryot Cell. 2009, 8 (3): 388-397. 10.1128/EC.00334-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Buchou T, Vernet M, Blond O, Jensen HH, Pointu H, Olsen BB, Cochet C, Issinger OG, Boldyreff B: Disruption of the regulatory beta subunit of PK CK2 in mice leads to a cell-autonomous defect and early embryonic lethality. Mol Cell Biol. 2003, 23 (3): 908-915. 10.1128/MCB.23.3.908-915.2003.PubMedPubMed CentralView ArticleGoogle Scholar
- Fraser AG, Kamath RS, Zipperlen P, Martinez-Campos M, Sohrmann M, Ahringer J: Functional genomic analysis of C. elegans chromosome I by systematic RNA interference. Nature. 2000, 408 (6810): 325-330. 10.1038/35042517.PubMedView ArticleGoogle Scholar
- Bidwai AP, Reed JC, Glover CV: Cloning and disruption of CKB1, the gene encoding the 38-kDa beta subunit of Saccharomyces cerevisiae casein kinase II (CKII). Deletion of CKII regulatory subunits elicits a salt-sensitive phenotype. J Biol Chem. 1995, 270 (18): 10395-10404. 10.1074/jbc.270.18.10395.PubMedView ArticleGoogle Scholar
- Bidwai AP, Reed JC, Glover CV: Casein kinase II of Saccharomyces cerevisiae contains two distinct regulatory subunits, beta and beta'. Arch Biochem Biophys. 1994, 309 (2): 348-355. 10.1006/abbi.1994.1123.PubMedView ArticleGoogle Scholar
- Ward P, Equinet L, Packer J, Doerig C: Protein kinases of the human malaria parasite Plasmodium falciparum: the kinome of a divergent eukaryote. BMC Genomics. 2004, 5: 79-10.1186/1471-2164-5-79.PubMedPubMed CentralView ArticleGoogle Scholar
- Anamika , Srinivasan N, Krupa A: A genomic perspective of PKs in Plasmodium falciparum. Proteins. 2005, 58 (1): 180-189.PubMedView ArticleGoogle Scholar
- Bozdech Z, Llinas M, Pulliam BL, Wong ED, Zhu J, DeRisi JL: The transcriptome of the intraerythrocytic developmental cycle of Plasmodium falciparum. PLoS Biol. 2003, 1 (1): E5-PubMedPubMed CentralView ArticleGoogle Scholar
- Le Roch KG, Zhou Y, Blair PL, Grainger M, Moch JK, Haynes JD, De La Vega P, Holder AA, Batalov S, Carucci DJ, et al: Discovery of gene function by expression profiling of the malaria parasite life cycle. Science. 2003, 301 (5639): 1503-1508. 10.1126/science.1087025.PubMedView ArticleGoogle Scholar
- Florens L, Washburn MP, Raine JD, Anthony RM, Grainger M, Haynes JD, Moch JK, Muster N, Sacci JB, Tabb DL, et al: A proteomic view of the Plasmodium falciparum life cycle. Nature. 2002, 419 (6906): 520-526. 10.1038/nature01107.PubMedView ArticleGoogle Scholar
- Canton DA, Zhang C, Litchfield DW: Assembly of PK CK2: investigation of complex formation between catalytic and regulatory subunits using a zinc-finger-deficient mutant of CK2beta. Biochem J. 2001, 358: (Pt 1):87-94.View ArticleGoogle Scholar
- Graham KC, Litchfield DW: The regulatory beta subunit of PK CK2 mediates formation of tetrameric CK2 complexes. J Biol Chem. 2000, 275 (7): 5003-5010. 10.1074/jbc.275.7.5003.PubMedView ArticleGoogle Scholar
- Sidhu AB, Valderramos SG, Fidock DA: pfmdr1 mutations contribute to quinine resistance and enhance mefloquine and artemisinin sensitivity in Plasmodium falciparum. Mol Microbiol. 2005, 57 (4): 913-926. 10.1111/j.1365-2958.2005.04729.x.PubMedView ArticleGoogle Scholar
- Johnson D, Gunther K, Ansorge I, Benting J, Kent A, Bannister L, Ridley R, Lingelbach K: Characterization of membrane proteins exported from Plasmodium falciparum into the host erythrocyte. Parasitology. 1994, 109 (Pt 1): 1-9.PubMedView ArticleGoogle Scholar
- Chandra BR, Olivieri A, Silvestrini F, Alano P, Sharma A: Biochemical characterization of the two nucleosome assembly proteins from Plasmodium falciparum. Mol Biochem Parasitol. 2005, 142 (2): 237-247. 10.1016/j.molbiopara.2005.04.006.PubMedView ArticleGoogle Scholar
- Navadgi VM, Chandra BR, Mishra PC, Sharma A: The two Plasmodium falciparum nucleosome assembly proteins play distinct roles in histone transport and chromatin assembly. J Biol Chem. 2006, 281 (25): 16978-16984. 10.1074/jbc.M602243200.PubMedView ArticleGoogle Scholar
- Chene A, Vembar SS, Riviere L, Lopez-Rubio JJ, Claes A, Siegel TN, Sakamoto H, Scheidig-Benatar C, Hernandez-Rivas R, Scherf A: PfAlbas constitute a new eukaryotic DNA/RNA-binding protein family in malaria parasites. Nucleic Acids Res. 2011Google Scholar
- Bell SD, Botting CH, Wardleworth BN, Jackson SP, White MF: The interaction of Alba, a conserved archaeal chromatin protein, with Sir2 and its regulation by acetylation. Science. 2002, 296 (5565): 148-151. 10.1126/science.1070506.PubMedView ArticleGoogle Scholar
- Bracchi-Ricard V, Barik S, Delvecchio C, Doerig C, Chakrabarti R, Chakrabarti D: PfPK6, a novel cyclin-dependent kinase/mitogen-activated PK-related PK from Plasmodium falciparum. Biochem J. 2000, 347 (Pt 1): 255-263.PubMedPubMed CentralView ArticleGoogle Scholar
- Le Roch K, Sestier C, Dorin D, Waters N, Kappes B, Chakrabarti D, Meijer L, Doerig C: Activation of a Plasmodium falciparum cdc2-related kinase by heterologous p25 and cyclin H. Functional characterization of a P. falciparum cyclin homologue. J Biol Chem. 2000, 275 (12): 8952-8958. 10.1074/jbc.275.12.8952.PubMedView ArticleGoogle Scholar
- Treeck M, Sanders JL, Elias JE, Boothroyd JC: The phosphoproteomes of Plasmodium falciparum and Toxoplasma gondii reveal unusual adaptations within and beyond the parasites' boundaries. Cell Host Microbe. 10 (4): 410-419.
- Duraisingh MT, Voss TS, Marty AJ, Duffy MF, Good RT, Thompson JK, Freitas-Junior LH, Scherf A, Crabb BS, Cowman AF: Heterochromatin silencing and locus repositioning linked to regulation of virulence genes in Plasmodium falciparum. Cell. 2005, 121 (1): 13-24. 10.1016/j.cell.2005.01.036.PubMedView ArticleGoogle Scholar
- Freitas-Junior LH, Hernandez-Rivas R, Ralph SA, Montiel-Condado D, Ruvalcaba-Salazar OK, Rojas-Meza AP, Mancio-Silva L, Leal-Silvestre RJ, Gontijo AM, Shorte S, et al: Telomeric heterochromatin propagation and histone acetylation control mutually exclusive expression of antigenic variation genes in malaria parasites. Cell. 2005, 121 (1): 25-36. 10.1016/j.cell.2005.01.037.PubMedView ArticleGoogle Scholar
- Tonkin CJ, Carret CK, Duraisingh MT, Voss TS, Ralph SA, Hommel M, Duffy MF, Silva LM, Scherf A, Ivens A, et al: Sir2 paralogues cooperate to regulate virulence genes and antigenic variation in Plasmodium falciparum. PLoS Biol. 2009, 7 (4): e84-10.1371/journal.pbio.1000084.PubMedView ArticleGoogle Scholar
- Roussou I, Draetta G: The Schizosaccharomyces pombe casein kinase II alpha and beta subunits: evolutionary conservation and positive role of the beta subunit. Mol Cell Biol. 1994, 14 (1): 576-586.PubMedPubMed CentralView ArticleGoogle Scholar
- Gyenis L, Litchfield DW: The emerging CK2 interactome: insights into the regulation and functions of CK2. Mol Cell Biochem. 2008, 316 (1-2): 5-14. 10.1007/s11010-008-9830-5.PubMedView ArticleGoogle Scholar
- Calvert ME, Keck KM, Ptak C, Shabanowitz J, Hunt DF, Pemberton LF: Phosphorylation by casein kinase 2 regulates Nap1 localization and function. Mol Cell Biol. 2008, 28 (4): 1313-1325. 10.1128/MCB.01035-07.PubMedPubMed CentralView ArticleGoogle Scholar
- Solyakov L, Halbert J, Alam MM, Semblat JP, Dorin-Semblat D, Reininger L, Bottrill AR, Mistry S, Abdi A, Fennell C, et al: Global kinomic and phospho-proteomic analyses of the human malaria parasite Plasmodium falciparum. Nat Commun. 2011, 2: 565-PubMedView ArticleGoogle Scholar
- Treeck M, Sanders JL, Elias JE, Boothroyd JC: The phosphoproteomes of Plasmodium falciparum and Toxoplasma gondii reveal unusual adaptations within and beyond the parasites' boundaries. Cell Host Microbe. 2011, 10 (4): 410-419. 10.1016/j.chom.2011.09.004.PubMedPubMed CentralView ArticleGoogle Scholar
- Scherf A, Lopez-Rubio JJ, Riviere L: Antigenic variation in Plasmodium falciparum. Annu Rev Microbiol. 2008, 62: 445-470. 10.1146/annurev.micro.61.080706.093134.PubMedView ArticleGoogle Scholar
- Oki M, Aihara H, Ito T: Role of histone phosphorylation in chromatin dynamics and its implications in diseases. Subcell Biochem. 2007, 41: 319-336.PubMedGoogle Scholar
- Cheung WL, Turner FB, Krishnamoorthy T, Wolner B, Ahn SH, Foley M, Dorsey JA, Peterson CL, Berger SL, Allis CD: Phosphorylation of histone H4 serine 1 during DNA damage requires casein kinase II in S. cerevisiae. Curr Biol. 2005, 15 (7): 656-660. 10.1016/j.cub.2005.02.049.PubMedView ArticleGoogle Scholar
- Chêne A, Vembar S, Rivière L, Lopez-Rubio JJ, Claes A, Siegel N, Scheidig-Benatar C, Hernandez-Rivas R, Scherf Artur: ALBA, a novel DNA/RNA-binding protein family of archaeal origin in malaria parasites. Nucl Acids Res. submitted
- Aravind L, Iyer LM, Anantharaman V: The two faces of Alba: the evolutionary connection between proteins participating in chromatin structure and RNA metabolism. Genome Biol. 2003, 4 (10): R64-10.1186/gb-2003-4-10-r64.PubMedPubMed CentralView ArticleGoogle Scholar
- Yun CY, Fu XD: Conserved SR PK functions in nuclear import and its action is counteracted by arginine methylation in Saccharomyces cerevisiae. J Cell Biol. 2000, 150 (4): 707-718. 10.1083/jcb.150.4.707.PubMedPubMed CentralView ArticleGoogle Scholar
- Haltiwanger BM, Matsumoto Y, Nicolas E, Dianov GL, Bohr VA, Taraschi TF: DNA base excision repair in human malaria parasites is predominantly by a long-patch pathway. Biochemistry. 2000, 39 (4): 763-772. 10.1021/bi9923151.PubMedView ArticleGoogle Scholar
- Trotta RF, Brown ML, Terrell JC, Geyer JA: Defective DNA repair as a potential mechanism for the rapid development of drug resistance in Plasmodium falciparum. Biochemistry. 2004, 43 (17): 4885-4891. 10.1021/bi0499258.PubMedView ArticleGoogle Scholar
- Tonkin CJ, van Dooren GG, Spurck TP, Struck NS, Good RT, Handman E, Cowman AF, McFadden GI: Localization of organellar proteins in Plasmodium falciparum using a novel set of transfection vectors and a new immunofluorescence fixation method. Mol Biochem Parasitol. 2004, 137 (1): 13-21. 10.1016/j.molbiopara.2004.05.009.PubMedView ArticleGoogle Scholar
- Reininger L, Billker O, Tewari R, Mukhopadhyay A, Fennell C, Dorin-Semblat D, Doerig C, Goldring D, Harmse L, Ranford-Cartwright L, et al: A NIMA-related PK is essential for completion of the sexual cycle of malaria parasites. J Biol Chem. 2005, 280 (36): 31957-31964. 10.1074/jbc.M504523200.PubMedView ArticleGoogle Scholar
- Walliker D, Quakyi IA, Wellems TE, McCutchan TF, Szarfman A, London WT, Corcoran LM, Burkot TR, Carter R: Genetic analysis of the human malaria parasite Plasmodium falciparum. Science. 1987, 236 (4809): 1661-1666. 10.1126/science.3299700.PubMedView ArticleGoogle Scholar
- Dorin D, Alano P, Boccaccio I, Ciceron L, Doerig C, Sulpice R, Parzy D: An atypical mitogen-activated PK (MAPK) homologue expressed in gametocytes of the human malaria parasite Plasmodium falciparum. Identification of a MAPK signature. J Biol Chem. 1999, 274 (42): 29912-29920. 10.1074/jbc.274.42.29912.PubMedView ArticleGoogle Scholar
- Lambros C, Vanderberg JP: Synchronization of Plasmodium falciparum erythrocytic stages in culture. J Parasitol. 1979, 65 (3): 418-420. 10.2307/3280287.PubMedView ArticleGoogle Scholar
- Dorin-Semblat D, Quashie N, Halbert J, Sicard A, Doerig C, Peat E, Ranford-Cartwright L, Doerig C: Functional characterization of both MAP kinases of the human malaria parasite Plasmodium falciparum by reverse genetics. Mol Microbiol. 2007, 65 (5): 1170-1180. 10.1111/j.1365-2958.2007.05859.x.PubMedView ArticleGoogle Scholar
- Lanzer M, de Bruin D, Ravetch JV: A sequence element associated with the Plasmodium falciparum KAHRP gene is the site of developmentally regulated protein-DNA interactions. Nucleic Acids Res. 1992, 20 (12): 3051-3056. 10.1093/nar/20.12.3051.PubMedPubMed CentralView ArticleGoogle Scholar
- Keller A, Nesvizhskii AI, Kolker E, Aebersold R: Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search. Anal Chem. 2002, 74 (20): 5383-5392. 10.1021/ac025747h.PubMedView ArticleGoogle Scholar
- Nesvizhskii AI, Keller A, Kolker E, Aebersold R: A statistical model for identifying proteins by tandem mass spectrometry. Anal Chem. 2003, 75 (17): 4646-4658. 10.1021/ac0341261.PubMedView ArticleGoogle Scholar
- Dorin D, Le Roch K, Sallicandro P, Alano P, Parzy D, Poullet P, Meijer L, Doerig C: Pfnek-1, a NIMA-related kinase from the human malaria parasite Plasmodium falciparum. Biochemical properties and possible involvement in MAPK regulation. Eur J Biochem. 2001, 268 (9): 2600-2608. 10.1046/j.1432-1327.2001.02151.x.PubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.