A carboxylesterase, Esterase-6, modulates sensory physiological and behavioral response dynamics to pheromone in Drosophila
© Chertemps et al; licensee BioMed Central Ltd. 2012
Received: 27 February 2012
Accepted: 21 June 2012
Published: 21 June 2012
Insects respond to the spatial and temporal dynamics of a pheromone plume, which implies not only a strong response to 'odor on', but also to 'odor off'. This requires mechanisms geared toward a fast signal termination. Several mechanisms may contribute to signal termination, among which odorant-degrading enzymes. These enzymes putatively play a role in signal dynamics by a rapid inactivation of odorants in the vicinity of the sensory receptors, although direct in vivo experimental evidences are lacking. Here we verified the role of an extracellular carboxylesterase, esterase-6 (Est-6), in the sensory physiological and behavioral dynamics of Drosophila melanogaster response to its pheromone, cis-vaccenyl acetate (cVA). Est-6 was previously linked to post-mating effects in the reproductive system of females. As Est-6 is also known to hydrolyze cVA in vitro and is expressed in the main olfactory organ, the antenna, we tested here its role in olfaction as a putative odorant-degrading enzyme.
We first confirm that Est-6 is highly expressed in olfactory sensilla, including cVA-sensitive sensilla, and we show that expression is likely associated with non-neuronal cells. Our electrophysiological approaches show that the dynamics of olfactory receptor neuron (ORN) responses is strongly influenced by Est-6, as in Est-6° null mutants (lacking the Est-6 gene) cVA-sensitive ORN showed increased firing rate and prolonged activity in response to cVA. Est-6° mutant males had a lower threshold of behavioral response to cVA, as revealed by the analysis of two cVA-induced behaviors. In particular, mutant males exhibited a strong decrease of male-male courtship, in association with a delay in courtship initiation.
Our study presents evidence that Est-6 plays a role in the physiological and behavioral dynamics of sex pheromone response in Drosophila males and supports a role of Est-6 as an odorant-degrading enzyme (ODE) in male antennae. Our results also expand the role of Est-6 in Drosophila biology, from reproduction to olfaction, and highlight the role of ODEs in insect olfaction.
Keywordscarboxylesterase esterase 6 olfaction pheromone signal termination
A sense that lacks spatial resolution requires a high temporal resolution for accurate location of signal sources in space. Insect pheromone responses exemplify this, with a capability of resolving and responding to pheromone filaments in 100 to 200 ms . For such a system to operate it requires not only fast responses to 'odor on', but also to 'odor off'. Odor-off responses imply inactivation of odorant signals. Several mechanisms have been proposed to participate in signal cessation or reduction within insect olfactory hairs (or sensilla), involving either olfactory receptors (Ors) or molecules interacting with them (reviewed in ). In Drosophila melanogaster, when Or genes were expressed in another olfactory receptor neuron (ORN) than their native ORN by using the 'empty neuron' system (Δhalo mutant), signal termination was similar to what had been observed in their native ORN, suggesting that Ors play a key role in signal dynamics . However, when an Or from the silk moth Bombyx mori was expressed in Drosophila T1 sensilla, termination of the response was rapid, whereas it was delayed when this receptor was expressed in another type of sensilla than the T1s, suggesting that the cellular environment of Ors could also play a role in the dynamics of the response . In particular, fast degradation of odorants in the vicinity of Ors by odorant-degrading enzymes (ODEs) has been proposed as a mechanism contributing to the termination of ORN responses. Pheromone degradation in vitro by antennal extracts (; reviewed in [6, 7]), by purified antennal enzymes [8, 9], as well as enzymatic inhibition in vivo (reviewed in [10, 11]) strongly support this hypothesis. Various enzyme families were described as candidate ODEs, such as carboxylesterases, aldehyde oxidases, epoxide hydrolases, glutathione-S-transferases or cytochrome P450 (reviewed in ). Few ODEs have been both identified at the molecular level and functionally characterized in vitro. Among them, carboxylesterases involved in pheromone/odorant degradation were the most studied [8, 9, 12–14]. However, involvement of ODEs in odorant processing has never been directly demonstrated in vivo.
In Drosophila melanogaster, the molecular mechanisms involved in the reception of a male-produced olfactory pheromone, cis-vaccenyl acetate (cVA), are intensely studied. In males, cVA suppresses male-male courtship  and promotes male-male aggression . cVA also suppresses courtship towards recently-mated females, as cVA is transferred to the female with the seminal fluid . Or67d, an Or mediating the sensory and behavioral responses to the cVA [15, 16, 18, 19] is expressed in T1 trichoid sensilla . LUSH, an odorant-binding protein (OBP), and SNMP1, a putative membrane bound coreceptor, are also required for cVA sensitivity [15, 18, 20–22]. Elements of binding and reception of cVA within T1 sensilla are thus well documented, but the mechanisms of cVA inactivation are unknown. In our search for putative factors that are involved in cVA degradation we noted that an extracellular carboxylesterase (EC 18.104.22.168), Esterase-6 (Est-6; CG6917), which is transferred during copulation to the female with the seminal fluid , hydrolyzes cVA in vitro . Interestingly in males, Est-6 is not only expressed in the ejaculatory duct , but also in the antennae [26–29], suggesting that Est-6 could play a role in pheromone processing.
In the present work we determined in vivo the role of Est-6 in cVA olfaction. We studied the phenotypes of several Est-6 mutant and control strains at the electrophysiological and behavioral levels. Our results demonstrate that Est-6 enables flies to detect and respond to the temporal dynamics of cVA stimulation. In addition, cVA-triggered behaviors are also modified in mutants, suggesting that Est-6 is of behavioral significance.
EST-6is highly and broadly expressed in male antennae
ORN responses to cVA depend on Est-6expression
Est-6modulates courtship behavior
We subsequently scored the CI of males to CS virgin females treated with exogenous cVA or solvent only. With solvent only, the CI was comparable in CS, Rescue and Est-6° males (53.4%, 50.8% and 52.8%, respectively; Figure 5A) and reached similar level as without any treatment. With the highest dose of cVA, the CI of control and Est-6° flies dropped to around 14%. The intermediate dose of 150 ng (approximately two-thirds male equivalent) induced a decrease of courtship in the three genotypes, however this decrease was more pronounced in Est-6 (19.2%) than in CS (39.8%) and Rescue (32,2%) males. This decrease was associated with a delay in courtship initiation, as shown by the corresponding wing vibration latency (Figure 5C). More interestingly, with the lowest dose (50 ng, 1/4 male equivalent) the CI of CS and Rescue males was unaffected, whereas it was significantly reduced in Est-6° males (30.4%), with again a delay in courtship initiation. A decrease in copulation attempts was observed when females were treated with cVA, but this decrease was comparable in the three strains (Figure 5B). The antiaphrodisiac effect of cVA was thus dose-dependent in the three strains. However, the amount of synthetic cVA required to inhibit the male courtship was lower for Est-6° than for control males, suggesting that the mutants presented a lowest threshold of response to the pheromone.
Est-6modulates aggression-promoting behavior
Olfactory behavior in response to food odors is not altered in mutant flies
The response of male flies to olfactory cues from food was evaluated using a trap assay . Mutant flies were able to detect and locate the food paste equally well as control males (Additional file 2, Figure S2A). As the performance in this trap assay is a good reflect of the olfactory function , the result indicates that the lack of Est-6 in the antennae or the genital tract does not have a general effect on olfactory-driven behaviors in mutants. Finally, the locomotor activity of mutant males was also comparable to control males, which demonstrates that Est-6 mutation does not affect locomotor activity in general (Additional file 2, Figure S2B).
This study demonstrates that a carboxylesterase, Est-6, previously linked to post-mating effects in the reproductive system of D. melanogaster females, plays also a role in the sensitivity and dynamics of ORNs tuned to cVA, the volatile fruit fly pheromone. We also infer that this physiological function of Est-6 at the peripheral olfactory level is required for normal male behavioral responses to cVA.
Est-6 is known as an extracellular enzyme in the male genital tract  and its extracellular location within the antennae has been shown by its isolation during the analysis of the soluble proteome of D. melanogaster antennae . We show here that Est-6 expression in male antennae is high and associated with most of olfactory sensilla, confirming the Est-6/lacZ pattern previously observed within the third antennal segment . In addition, we found that Est-6 expression is mostly associated with the accessory cells embedding the sensory neurons and located at the base of the sensilla. These cells are already known to produce OBPs and secrete them into the sensillum lymph [35–37]. Altogether, these data suggest that Est-6 could be secreted within the lymph of the olfactory sensilla, including cVA-sensitive sensilla.
As all other β-esterases, Est-6 clustered within a clade that includes extracellular catalytically competent esterases (reviewed in ). Phylogenetic analyses [13, 39] also revealed that Est-6 was closely related to the antennal carboxylesterases characterized in vitro in the wild silk moth Antheraea polyphemus [9, 12] and in the beetle Popilia japonica . These ODEs were able to hydrolyze the female sex pheromones in vitro with kinetics suggesting that they could play a significant role in the dynamic of signal termination in vivo [8, 9, 12]. cVA degradation by purified Est-6 into cis-vaccenyl alcohol (cVOH) has been shown in vitro  and it has been also demonstrated that cVOH elicits only very low responses in T1 sensory neurons . Together with its sensillar location, this catalytic activity towards the pheromone suggested that Est-6 could a play a role in pheromone signal termination, as a candidate ODE. Further determination of Est-6 kinetics towards cVA will be useful to precise its mode of action.
If ODEs were required for odorant processing, then their inhibition should disturb odorant reception within the antennae. Several pharmacological approaches have been used to address this question. Volatile trifluoroketones (TFKs), which can inhibit carboxylesterase activities  were used in several lepidopteran species to test their effect on pheromone response , but controversial effects were observed. In the moth Ostrinia nubilalis, prolonged repolarization time of EAG in response to the pheromone after TFK application suggested that esterases were involved in pheromone deactivation , but it has also been suggested that TFKs may interact with Ors, OBPs or other members of the transduction cascade [42, 43]. Inhibition of antennal cytochrome P450 by metyrapone reduces pheromone responses in a scarab beetle, suggesting that these intracellular enzymes were required for maintaining olfactory sensitivity .
In Drosophila, genetic tools offer the opportunity to knockdown candidate genes specifically to verify their physiological role directly. This approach led us to demonstrate that the absence of Est-6 in males indeed modifies neuronal responses to the pheromone, with stronger and longer-lasting responses. Noteworthy, the kinetics of signal termination within Est-6° T1 sensilla is altered even at physiological low doses of pheromone and with brief stimulations, as expected after the knockdown of an ODE. We can assume that the lack of Est-6 in mutant antennae prevents the degradation of cVA, which could lead to an accumulation of cVA in the perireceptor space of T1 sensilla. While binding with cVA, the OBP LUSH encounters a conformational change and the LUSH/cVA complex would be the active form that interacts with the receptor (Laughlin et al., 2008). LUSH increases the sensitivity of T1 ORN to cVA but does not to cVOH . In Est-6° mutant antennae, accumulation of cVA would thus lead to an accumulation of the complex OBP/pheromone, leading to stronger responses and delayed signal termination. However, Est-6 involvement in signal dynamics does not preclude a role of additional mechanisms in signal termination.
As Est-6 has a physiological effect on cVA reception, we thus tested whether Est-6 mutation could influence cVA-triggered behaviors. We found that Est-6 deficiency clearly enhances the antiaphrodisiac effect of the pheromone. Topical applications of exogenous cVA on females reduced male courtship as already observed . In addition, we showed that the effect of exogenous cVA is dose dependent in control and Est-6° males. However, the threshold of behavioral response to the pheromone is lower in Est-6° males. Compared to control flies, lower doses of cVA were sufficient to slow down courtship initiation of mutant males, thus to reduce their courtship. Est-6 deficiency also increases dispersal thus likely aggression. The proximity to a high density of male flies has been shown to increase the level of male aggression, thus dispersal, in a dose-dependent manner . Dispersal of Est-6° males in absence of exogenous cVA suggests again a lower threshold of behavioral response in mutant males.
Activation by cVA of ORNs carrying Or67d in T1 sensilla is sufficient to inhibit male-male courtship behavior , and to promote cVA-induced aggression . In particular, increasing artificially the excitability of Or67d-expressing ORNs, by expressing a bacterially-derived sodium channel, promotes dispersal of grouped flies even in absence of exogenous cVA . Modified physiological responses of T1 sensilla to cVA in Est-6° mutants could thus potentially account for the observed exacerbated behaviors. Delayed cVA termination and stronger responses of T1 sensilla from Est-6° mutant males are consistent with their lower threshold of behavioral response. Indirect evidence for a function specific for T1 sensilla comes from the fact that lack of Est-6 in mutants did not impair other chemically-driven behaviors, as indicated by a normal response to food odors and to female pheromones.
In conclusion, we have demonstrated that an extracellular esterase, Est-6, is involved in maintaining proper temporal dynamics of cVA detection at the peripheral olfactory circuit level and is involved in cVA-induced behaviors in males. These results expand the role of Est-6 in Drosophila biology, from reproduction to olfaction. After its transfer during mating with the semen, Est-6 is known to rapidly translocate to the female hemolymph and to impact female reproductive behavior (stimulation of egg laying and inhibition of receptivity for remating) . Our results demonstrate that the same enzyme plays a crucial role in cVA detection in male antennae. This work also highlights the physiological role of carboxylesterases in insect odorant reception in vivo. In vertebrates, a potential role of extracellular enzymes from the nasal mucus has been recently revealed by a pharmacological inhibition approach . Enzymatic conversion of odorants seemed to be fast enough to affect olfactory dynamics. The corresponding enzymes were not characterized, but carboxylesterases were suspected to play a role in ester conversion . Enzyme-based mechanism of inactivation could be comparable in olfactory systems of insects and vertebrates.
In a context of pest insect management, these enzymes may be interesting targets for the development of specific inhibitors that interfere with the insect's ability to respond adequately to olfactory cues from mates or host plants.
Fly strains, rearing and tissue collection
The following strains were used during this study: an Est-6° null mutant strain (Bloomington stock 4211), completely lacking Est-6 and described in detail ; a rescue strain described in Odgers et al. , which presents a similar genetic background as Est-6° (the Est-6 promoter was fused to the Est-6 coding region and transformed into the Est-6 null background); Canton-S (CS) flies were used as wild-type control flies. Transgenic UAS-mCD8::GFP (Bloomington stock 5130) flies were used for immunohistochemistry experiments to determine more precisely the expression pattern of Est-6 within antennae. For the generation of the Est-6Gal4 lines, a 1,132 fragment corresponding to the Est-6 promoter region was cloned in the pChs-Gal4 vector, and transgenic flies were generated by P-mediated germline transformation by BestGene Inc. (Chino Hill, CA, USA) according to standard procedures . All flies were raised at 25°C on standard yeast/cornmeal/agar medium in a 12-h light/12-h dark cycle, 50% to 60% relative humidity.
To precisely define the levels of Est-6 expression in antennae and other chemosensory appendages, Est-6 transcripts were quantified by qPCR analysis. Antennae from 5 to 7-day old males and females, male legs and proboscis with maxillary palps were dissected for total RNA extraction using TRIzol Reagent (Invitrogen, Carlsbad, CA, USA). Heads without appendages were also tested. Single-stranded cDNAs were synthesized from total RNAs (1 μg) using Superscript II reverse transcriptase (Invitrogen). All reactions were performed as previously described  on the LightCycler® 480 Real-Time PCR System (Roche, Basel, Switzerland). Each reaction was run in triplicate with at least three independent biological replicates. The pgk, rpl8 and rp49 genes were used as reference genes. Specific primers were designed using EPRIMER3 http://mobyle.pasteur.fr and were as follows: rp49up CGGATCGATATGCTAAGCTGT, rp49do ACGTTGTGCACCAGGAACTT, rpL8up TCGTATCGACAAGCCCATCCTGA, rpl8do ACCACGGATCCTACCGGTACGAC, pgkup CGAGAAACTGGTGGAGAAGG, pgkdo CGAAGTTGGGGAACTCAAAG, Est6up TTCCCGGAAACTATGGACTG and Est6do CAGTTCAAAGGCTCGTCCTC. Normalized Est-6 expression was calculated with Q-Gene software .
Localization of Est-6expression within antennae
To localize the expression site of Est-6 in the antenna, we used transgenic flies expressing GFP under the control of Est-6 promoter and we performed immunohistochemistry with an anti-ELAV antibody as neuronal marker. Heads with antennae from 5-day-old males Est-6 Gal4 /UAS-mCD8-GFP were fixed for 3 h in 4% paraformaldehyde with 0.2% Triton X-100 (Sigma-Aldrich, Saint-Quentin Fallavier, France), then washed for 1 h with phosphate-buffered saline containing 0.2% Triton X-100 (PBST, Sigma-Aldrich). Heads were then embedded in Tissue-Tek™ (CellPath, Newtown Powys, UK) and cryosections (15 μm) were set in cell culture insert (Greiner Bio-one, Monroe, LA, USA). After blocking with 3% normal goat serum and 1% bovine serum albumin (BSA) in PBST (1 h at room temperature (RT)), an anti-ELAV (from Developmental Studies Hybridoma Bank, University of Iowa) was diluted 1:10 (v/v) in the blocking solution (3% normal goat serum in PBST) and incubated overnight at RT. After a brief rinse in PBST, an anti-mouse conjugated Alexa-546 (Invitrogen) was applied 1:250 (v/v) in the blocking solution for 4 h at RT. tissues were mounted in Slowfade reagent (Invitrogen). Imaging was performed on Olympus BX61 microscope with a ScopePro software.
Comparison of antennal responses to cVA by EAG
EAG recordings were performed at RT on 5-day-old males previously kept in individual tubes, as described previously . Reference and recording glass capillary electrodes were filled with 120 mM NaCl, 5 mM KCl, 1 mM CaCl2, 4 mM MgCl2, 10 mM 4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid (HEPES, Sigma-Aldrich), pH = 7.2. The reference electrode (approximately 1 μm tip diameter) was inserted in one eye and the recording electrode (approximately 10 μm tip diameter) was pushed against one antenna. The signal was amplified (× 500) and low pass filtered online (10 kHz) with an Axopatch 200B amplifier (Molecular Devices, Union City, CA, USA) and digitized at 1 kHz with a Digidata 1440A acquisition board (Molecular Devices). A dose-response curve was established to select a dose of cVA that induces a high and saturating response. Antennae were then stimulated for 5 s with either pure hexane (> 98% purity, Carlo-Erba Reagents, Val de Reuil, France) as negative control, or with cVA (diluted in hexane, 200 μg/cartridge), or with 2-heptanone (1:1,000 in paraffin oil, > 98% purity, Carlo-Erba) as positive control. Analysis of EAGs was carried out under pClamp 10 (Molecular Devices). Repolarization rates were compared between the different strains. Repolarization rate was defined as: ((maximum amplitude of depolarization - amplitude of depolarization at the end of stimulation) ÷ maximum amplitude of depolarization) × 100.
Analyses of T1 sensilla responses by single-sensillum recordings
Single-sensillum recordings were performed as described previously  in order to follow the response of T1 sensilla more precisely. In brief, a fly was restrained, a reference electrode was placed in the eye, and the recording tungsten electrode was brought in contact with the base of a sensillum. Signal was amplified (× 1,000, Syntech UN 06, Hilversum, The Netherlands). Actions potentials were analyzed offline with Autospike software (v. 4.0, Syntech). Three doses of cVA were tested, 0.5 μg, 5 μg and 50 μg, and two stimulus durations, 0.5 s and 3 s. Responses from individual ORNs were calculated as the increase in spike frequency relative to the prestimulus frequency. Average firing activities during the stimulus duration, as well as during the 9 s period following the end of stimulus were calculated.
Males were isolated after emergence and raised in individual tubes to avoid social interactions. All experiments were done under dim red lights at 25°C (50% to 60% relative humidity) and with immobilized target so as to enhance the behavioral effects of pheromone cues . For male-male assays, a single male (5 to 7 days old) was placed in a test chamber (3 cm diameter, 0.5 cm height) for 10 minutes before introducing a decapitated CS 'target' male. Courtship behavior was observed over 10 minutes and a courtship index (CI) was calculated. CI is the fraction of time spent in courtship activity in the 10-minute observation period. For each tested male, the latency before the first wing vibration and the number of copulation attempts were noted, as an indicator of courtship vigor. Heterosexual courtship was first measured in the same conditions as described for male-male courtship assay, except that a decapitated CS 'target' virgin female was introduced in the test chamber. Synthetic cVA was then applied on the dorsal abdomen of decapitated virgin females before to offer them to mutant or control males. Mature male flies contain approximately 1 μg of cVA in their ejaculatory bulb  and 400 ng on their cuticle . The quantity of cVA transferred to the female during copulation was estimated to 200 ng  but 70% of the pheromone is lost 6 h after insemination . The amount of cVA on females 24 h after mating was indeed only 10 ng . Three doses of cVA diluted in acetone (> 98% purity, Sigma-Aldrich) were applied on females, 400 ng (twice male equivalent), 150 ng (approximately two-thirds male equivalent) and 50 ng (one-quarter male equivalent).
Experiments were performed as described previously . Briefly, 15 males were raised together after emergence for 5 to 7 days. Six males were simultaneously introduced in a plastic tube (2.5 cm diameter, 10 cm height) coated with Fluon (Sigma-Aldrich) and a small cup containing food was placed in the center, together with a small piece of filter paper containing either 500 μg of synthetic cVA or acetone (solvent). Flies were videotaped for 30 minutes and the total number of flies on the food cup was counted every 30 s.
Control of olfactory behavior and of locomotor activity
The response of male flies to airborne chemicals was evaluated using the principle of the olfactory trap assay , which allows to test the ability of flies to detect and migrate toward a source of olfactory attractant. All tests were performed under dim red lights as for courtship assays. Ten flies of the same genotype (CS or Est-6° males) were placed in a 50 ml Greiner tube along with a trap constructed from a microfuge tube and two micropipette tips. Traps contained either a fresh yeast paste included in 10% agar as attractant or only agar. The number of trapped flies was counted after 22 h.
To verify that Est-6° males were not impaired in their mobility, their locomotor activity was determined as previously described . In brief, a single male was placed in the test chamber containing a filter paper with a bisecting line. The number of times the male crossed the line in a 3-minute observation period was counted.
Analyses were performed with Statistica 7 (StatSoft Inc., Tulsa, OK, USA). The Student's t test or Mann-Whitney U test were used for pairwise comparisons and analysis of variance (ANOVA) followed by a Fisher post-hoc test was performed for comparisons among multiple groups. P < 0.05 was accepted as statistically significant.
We warmly thank C Coppin for providing the Est-6 rescue flies, C Wicker-Thomas for the CS strain and BestGene for the transgenic flies. We thank Professor R Vogt for helpful discussions, and M Solvar and Coline Sentenac for technical help. This work was supported by Université Pierre et Marie Curie (UPMC), INRA, CSIC, SLU and a fellowship from the Research Ministry to ND.
- Baker TC, Vogt RG: Measured behavioural latency in response to sex-pheromone loss in the large silk moth Antheraea polyphemus. J Exp Biol. 1988, 137: 29-38.PubMed
- Rutzler M, Zwiebel L: Molecular biology of insect olfaction: recent progress and conceptual models. J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2005, 191: 777-790. 10.1007/s00359-005-0044-y.PubMedView Article
- Hallem EA, Ho MG, Carlson JR: The molecular basis of odor coding in the Drosophila antenna. Cell. 2004, 117: 965-979. 10.1016/j.cell.2004.05.012.PubMedView Article
- Syed Z, Ishida Y, Taylor K, Kimbrell D, Leal W: Pheromone reception in fruit flies expressing a moth's odorant receptor. Proc Natl Acad Sci USA. 2006, 103: 16538-16543. 10.1073/pnas.0607874103.PubMedPubMed CentralView Article
- Vogt RG, Riddiford LM: Pheromone binding and inactivation by moth antennae. Nature. 1981, 293: 161-163. 10.1038/293161a0.PubMedView Article
- Vogt RG: Molecular basis of pheromone detection in insects. Comprehensive Insect Physiology, Biochemistry, Pharmacology and Molecular Biology. Edited by: Gilbert L, Iatrou K, Gill S. 2005, London, UK: Elsevier, 3: 753-804.
- Jacquin-Joly E, Maïbèche-Coisne M: Molecular mechanisms of sex pheromone reception in Lepidoptera. Short Views on Insect Molecular Biology. Edited by: Chandrasekar R. 2009, Tamil Nadu, India: Bharathidasan University, 147-158.
- Ishida Y, Leal WS: Chiral discrimination of the Japanese beetle sex pheromone and a behavioral antagonist by a pheromone-degrading enzyme. Proc Natl Acad Sci USA. 2008, 105: 9076-9080. 10.1073/pnas.0802610105.PubMedPubMed CentralView Article
- Ishida Y, Leal WS: Rapid inactivation of a moth pheromone. Proc Natl Acad Sci USA. 2005, 102: 14075-14079. 10.1073/pnas.0505340102.PubMedPubMed CentralView Article
- Vogt RG: Biochemical diversity of odor detection: OBPs, ODEs and SNMPs. Insect Pheromone Biochemistry and Molecular Biology. Edited by: Blomquist GJ, Vogt RG. 2003, Oxford, UK: Elsevier, 391-445.View Article
- Maïbèche-Coisne M, Nikonov AA, Ishida Y, Jacquin-Joly E, Leal WS: Pheromone anosmia in a scarab beetle induced by in vivo inhibition of a pheromone-degrading enzyme. Proc Natl Acad Sci USA. 2004, 101: 11459-11464. 10.1073/pnas.0403537101.PubMedPubMed CentralView Article
- Vogt RG, Riddiford LM, Prestwich GD: Kinetic properties of a sex pheromone-degrading enzyme: the sensillar esterase of Antheraea polyphemus. Proc Natl Acad Sci USA. 1985, 82: 8827-8831. 10.1073/pnas.82.24.8827.PubMedPubMed CentralView Article
- Durand N, Carot-Sans G, Chertemps T, Bozzolan F, Party V, Renou M, Debernard S, Rosell G, Maïbèche-Coisne M: Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant. PLoS One. 2010, 5: e15026-10.1371/journal.pone.0015026.PubMedPubMed CentralView Article
- Durand N, Carot-Sans G, Bozzolan F, Rosell G, Siaussat D, Debernard S, Chertemps T, Maïbèche-Coisne M: Degradation of pheromone and plant volatile components by a same odorant-degrading enzyme in the cotton leafworm, Spodoptera littoralis. PLoS One. 2011, 6: e29147-10.1371/journal.pone.0029147.PubMedPubMed CentralView Article
- Kurtovic A, Widmer A, Dickson BJ: A single class of olfactory neurons mediates behavioural responses to a Drosophila sex pheromone. Nature. 2007, 446: 542-546. 10.1038/nature05672.PubMedView Article
- Wang L, Anderson D: Identification of an aggression-promoting pheromone and its receptor neurons in Drosophila. Nature. 2010, 463: 227-231. 10.1038/nature08678.PubMedPubMed CentralView Article
- Jallon J, Antony C, Benamar O: Un anti-aphrodisiaque produit par les mâles de Drosophila melanogaster et transféré aux femelles lors de la copulation. C R Acad Sci. 1981, 292: 1147-1149.
- Ha TS, Smith DP: A pheromone receptor mediates 11-cis-vaccenyl acetate-induced responses in Drosophila. J Neurosci. 2006, 26: 8727-8733. 10.1523/JNEUROSCI.0876-06.2006.PubMedView Article
- Ronderos D, Smith D: Activation of the T1 neuronal circuit is necessary and sufficient to induce sexually dimorphic mating behavior in Drosophila melanogaster. J Neurosci. 2010, 17: 2595-2599.View Article
- Jin X, Ha T, Smith D: SNMP is a signaling component required for pheromone sensitivity in Drosophila. Proc Natl Acad Sci USA. 2008, 105: 10996-11001. 10.1073/pnas.0803309105.PubMedPubMed CentralView Article
- Laughlin JD, Ha TS, Jones DN, Smith DP: Activation of pheromone-sensitive neurons is mediated by conformational activation of pheromone-binding protein. Cell. 2008, 133: 1255-1265. 10.1016/j.cell.2008.04.046.PubMedPubMed CentralView Article
- Benton R, Vannice K, Vosshall L: An essential role for a CD36-related receptor in pheromone detection in Drosophila. Nature. 2007, 450: 289-293. 10.1038/nature06328.PubMedView Article
- Richmond R, Gilbert D, Sheehan K, Gromko M, Butterworth F: Esterase 6 and reproduction in Drosophila melanogaster. Science. 1980, 207: 1483-1485. 10.1126/science.6767273.PubMedView Article
- Mane S, Tompkins M, Richmond R: Male esterase 6 catalyzes the synthesis of a sex pheromone in Drosophila melanogaster females. Science. 1983, 28: 419-421.View Article
- Sheehan K, Richmond R, Cochrane B: Studies of esterase 6 in Drosophila melanogaster. III. The developmental pattern and tissue distribution. Insect Biochem. 1979, 9: 443-450. 10.1016/0020-1790(79)90062-3.View Article
- Brady J, Richmond R, Oakeshott J: Cloning of the esterase-5 locus from Drosophila pseudoobscura and comparison with its homologue in D. melanogaster. Mol Biol Evol. 1990, 7: 525-546.PubMed
- Healy M, Dumancic M, Oakeshott J: Biochemical and physiological studies of soluble esterases from Drosophila melanogaster. Biochem Genet. 1991, 29: 365-388. 10.1007/BF00554144.PubMedView Article
- Tamarina N, Ludwig M, Richmond R: Divergent and conserved features in the spatial expression of the Drosophila pseudoobscura esterase-5B gene and the esterase-6 gene of Drosophila melanogaster. Proc Natl Acad Sci USA. 1997, 94: 7735-7741. 10.1073/pnas.94.15.7735.PubMedPubMed CentralView Article
- Anholt R, Williams T: The soluble proteome of the Drosophila antennae. Chem Senses. 2010, 35: 21-30. 10.1093/chemse/bjp073.PubMedPubMed CentralView Article
- Vosshall LB, Stocker RF: Molecular architecture of smell and taste in Drosophila. Ann Rev Neurosci. 2007, 30: 505-533. 10.1146/annurev.neuro.30.051606.094306.PubMedView Article
- de Bruyne M, Foster K, Carlson J: Odor coding in the Drosophila antenna. Neuron. 2001, 30: 537-552. 10.1016/S0896-6273(01)00289-6.PubMedView Article
- Ejima A, Smith B, Lucas C, van der Goes van Naters W, Miller C, Carlson J, Levine J, Griffith L: Generalization of courtship learning in Drosophila is mediated by cis-vaccenyl acetate. Curr Biol. 2007, 17: 599-605. 10.1016/j.cub.2007.01.053.PubMedPubMed CentralView Article
- Grosjean Y, Guenin L, Bardet H, Ferveur J: Prospero mutants induce precocious sexual behavior in Drosophila males. Behav Genet. 2007, 37: 575-584. 10.1007/s10519-007-9152-5.PubMedView Article
- Woodard C, Huang T, Sun H, Helfand S, Carlson J: Genetic analysis of olfactory behavior in Drosophila: a new screen yields the ota mutants. Genetics. 1989, 123: 315-326.PubMedPubMed Central
- Steinbrecht R, Ozaki M, Ziegelberger G: Immunocytochemical localization of pheromone-binding protein in moth antennae. Cell Tissue Res. 1992, 270: 287-302. 10.1007/BF00328015.View Article
- Kim M-S, Repp A, Smith DP: LUSH odorant-binding protein mediates chemosensory responses to alcohols in Drosophila melanogaster. Genetics. 1988, 150: 711-721.
- Galindo K, Smith D: A large family of divergent Drosophila odorant-binding proteins expressed in gustatory and olfactory sensilla. Genetics. 2001, 159: 1059-1072.PubMedPubMed Central
- Oakeshott J, Claudianos C, Campbell P, Newcomb R, Russell R: Biochemical genetics and genomics of insect esterases. Comprehensive Molecular Insect Science. Edited by: Gilbert L, Iatrou K, Gill S. 2005, Oxford, UK: Elsevier, 5: 309-381.View Article
- Tsubota T, Shiotsuki T: Genomic analysis of carboxyl/cholinesterase genes in the silkworm Bombyx mori. BMC Genomics. 2010, 11: 377-387. 10.1186/1471-2164-11-377.PubMedPubMed CentralView Article
- van der Goes van Naters W, Carlson J: Receptors and neurons for fly odors in Drosophila. Curr Biol. 2007, 17: 606-612. 10.1016/j.cub.2007.02.043.PubMedPubMed CentralView Article
- Renou M, Lucas P, Malo E, Quero C, Guerrero A: Effects of trifluoromethyl ketones and related compounds on the EAG and behavioural responses to pheromones in male moths. Chem Senses. 1997, 22: 407-416. 10.1093/chemse/22.4.407.PubMedView Article
- Pophof B: Inhibitors of sensillar esterase reversibly block the responses of moth pheromone receptor cells. J Comp Physiol A. 1998, 183: 153-164. 10.1007/s003590050243.View Article
- Pophof B, Gebauer T, Ziegelberger G: Decyl-thio-trifluoropropanone, a competitive inhibitor of moth pheromone receptors. J Comp Physiol A. 2000, 186: 315-323. 10.1007/s003590050432.PubMedView Article
- Nagashima A, Touhara K: Enzymatic conversion of odorants in nasal mucis affects olfactory glomerular activation patterns and odor perception. J Neurosci. 2010, 30: 16391-16398. 10.1523/JNEUROSCI.2527-10.2010.PubMedView Article
- Odgers W, Aquadro C, Coppin C, Healy M, Oakeshott J: Nucleotide polymorphism in the Est6 promoter, which is widespread in derived populations of Drosophila melanogaster, changes the level of esterase 6 expressed in the male ejaculatory duct. Genetics. 2002, 162: 785-797.PubMedPubMed Central
- Rubin G, Spradling A: Genetic transformation of Drosophila with transposable element vectors. Science. 1982, 218: 348-353. 10.1126/science.6289436.PubMedView Article
- Durand N, Carot-Sans G, Chertemps T, Montagné N, Jacquin-Joly E, Debernard S, Maïbèche-Coisne M: A diversity of putative carboxylesterases is expressed in the antennae of the noctuid moth Spodoptera littoralis. Insect Mol Biol. 2010, 19: 87-97.PubMedView Article
- Simon P: Q-gene: processing quantitative real-time RT-PCR data. Bioinformatics. 2003, 19: 1439-1440. 10.1093/bioinformatics/btg157.PubMedView Article
- Rollmann S, Mackay T, Anholt R: Pinocchio, a novel protein expressed in the antenna, contributes to olfactory behavior in Drosophila melanogaster. J Neurobiol. 2005, 63: 146-158. 10.1002/neu.20123.PubMedView Article
- Stensmyr M, Dekker T, Hansson B: Evolution of the olfactory code in the Drosophila melanogaster subgroup. Proc Biol Sci. 2003, 270: 2333-2340. 10.1098/rspb.2003.2512.PubMedPubMed CentralView Article
- Bartelt RJ, Schaner AM, Jackson LL: Cis-vaccenyl acetate as an aggregation pheromone in Drosophila melanogaster. J Chem Ecol. 1985, 11: 1747-1756. 10.1007/BF01012124.PubMedView Article
- Wang L, Han X, Mehren J, Hiroi M, Billeter J, Miyamoto T, Amrein H, Levine J, Anderson D: Hierarchical chemosensory regulation of male-male social interactions in Drosophila. Nat Neurosci. 2011, 14: 757-762. 10.1038/nn.2800.PubMedPubMed CentralView Article
- Villella A, Gailey D, Berwald B, Ohshima S, Barnes P, Hall J: Extended reproductive roles of the fruitless gene in Drosophila melanogaster revealed by behavioral analysis of new fru mutants. Genetics. 1997, 147: 1107-1130.PubMedPubMed Central
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.