RNAi in the regulation of mammalian viral infections
© Jeang; licensee BioMed Central Ltd. 2012
Received: 10 April 2012
Accepted: 22 June 2012
Published: 26 June 2012
Although RNA interference (RNAi) is known to play an important part in defense against viruses of invertebrates, its contribution to mammalian anti-viral defense has been a matter of dispute. This is surprising because all components of the RNAi machinery necessary for robust RNAi-mediated restriction of viruses are conserved in mammals, and the introduction of synthetic small interfering RNAs (siRNAs) into cells efficiently silences the replication of viruses that contain siRNA complementary sequences in those cells. Here, I discuss the reasons for the dispute, and review the evidence that RNAi is a part of the physiological defense of mammalian cells against viral infections.
RNA interference (RNAi) is a process in which small non-coding RNAs (of endogenous or exogenous origin) are incorporated into a multi-protein RNA-induced silencing complex (RISC) in cells to silence the expression of a sequence-homologous target RNA . Three major types of small non-coding RNAs function as RNAi: the piRNAs (PIWI-interacting RNAs), miRNAs (microRNAs), and siRNAs (small interfering RNAs). piRNAs and miRNAs are endogenous, small non-coding RNAs transcribed from cellular loci and then processed to generate fragments that engage with the downstream silencing machinery. Until recently, siRNAs were thought to be exclusively processed from the exogenous RNA of pathogens (for example, viruses) that infect the cell, but that view changed with the discovery of abundantly expressed endogenous siRNAs (endo-siRNAs) in animal cells [2, 3]. Currently, mammals are known to have hundreds of thousands of different piRNAs, produced from gene clusters of repetitive elements, and more than 1,000 different miRNAs; the number of endo-siRNAs still needs to be fully clarified.
One of the earliest descriptions of RNAi was in Caenorhabditis elegans. Early on, it was found that mutations affecting RNAi function in C. elegans and Drosophila melanogaster produced apparently normal organisms, but that these mutations increased the susceptibility of mutated animals to infection by viruses [5, 6]. These findings suggested an evolutionary role for RNAi in the defense of cells against pathogenic viral infections. Indeed, this rationale is consistent with the role of RNAi in post-transcriptional gene silencing of plant viruses  and with the conservation of an RNAi-like antiviral defense mechanism using small CRISPR (clusters of regularly interspaced short palindromic repeats) RNAs in prokaryotes . The need for conservation of an analogous antiviral system in vertebrates, however, has been questioned on the grounds of their advanced adaptive immunity to viral (and non-viral) pathogens and on the emergence of an interferon-based defense mechanism. Silencing mechanisms that depend on RNAi are, however, already known to operate in vertebrates to protect the germline DNA from transposons and endogenous retroviruses; and as discussed below, they appear also to operate in somatic cells.
RNAi and regulation of retroelements and viruses
Non-virologists are often surprised to learn that nearly 50% of the human genome is made up of virus-like transposable elements (TEs) , which are composed mostly of retrotransposons replicated through reverse transcription, and DNA transposons propagated through a cut-and-paste mechanism. Included among the TEs in the mouse and human genomes are distinctly recognizable endogenous retroviruses (ERVs; integrated retrovirus sequences that have entered the germline), with 5 to 8% of human DNA estimated to consist of human endogenous retrovirus (HERV) elements that segregate into 26 phylogenetically distinct retroviral lineages . These ERVs are likely to be fossilized remnants of anciently endogenized virus infections. While active human ERVs are rare , human non-ERV TEs (for example, short interspersed elements - SINES - and long interspersed elements - LINES) remain active for transposition, accounting for 1 new insertion every 100 to 200 human births or roughly 1 in every 1,000 human genetic mutations . By contrast, mouse ERVs are numerous and highly active and cause approximately 10% of spontaneous mutations in inbred mice .
Active replication of ERVs and virus-like elements needs to be suppressed in the germ line because they cause novel deleterious germline mutations. In mouse germ cells, piRNA-mediated silencing has been shown to be important for repressing TE activity . However, ERV and retrotransposon activities are not limited to the germline; they also occur in somatic cells where they can induce disease, in particular ERV- and retrotransposition-associated cancers [15, 16]. This raises the question of whether mechanisms also exist to protect somatic tissues. There is evidence for two such mechanisms. First, somatic cell endo-siRNAs, as recently described, may act to control ERV and TE activity . Second, emerging data have unexpectedly revealed that piRNAs are not confined to germline cells, but are also abundant in the somatic tissues of fruitfly, mouse, and rhesus macaques , in the neurons of Aplysia , and in a human T cell line . PIWI mRNA and MIWI protein have also been detected in macaque and mouse somatic tissues . If these new discoveries are confirmed, then ERV/TE-suppression in somatic cells may be mediated by piRNA-RISC.
There is also direct evidence supporting a role for RNAi in regulating viral infections in mammalian cells. As in the early studies that showed RNAi pathway mutations in C. elegans and D. melanogaster increased these organisms' susceptibility to infection by viruses [5, 6], mutations in or perturbation of RNAi pathway components in mouse , monkey cells  or human cells [22, 23] increase the replication of vesicular stomatitis virus (VSV), influenza A virus, and human immunodeficiency virus (HIV-1), respectively.
miRNA-mediated regulation of viral infection
The human genome encodes more than 1,000 different miRNAs; these miRNAs and their miRNA-RISC complexes recognize RNA targets through imperfect base-pairing . In silico analyses based on complementarity of miRNAs and their putative mRNA targets have led to estimates that miRNAs may regulate up to 30% of protein-coding human mRNAs. Not surprisingly, early analyses of the more than 1,000 human miRNA sequences aligned against a large dataset of pathogenic mammalian viral genomes indicated that most, if not all, viruses are recognized by one or more cellular miRNAs .
Efficient processing of viral short hairpin RNAs and miRNAs occurs in mammalian cells
Despite the above findings, there remains some contention about the physiological function of RNAi in regulating viruses in mammalian cells. This contention may be caused in part by expectations of mammalian cells/mammalian viruses based on results from invertebrate cells/invertebrate viruses. For instance, when invertebrate viruses infect mosquito cells, the incoming viral dsRNAs or replication intermediates are frequently processed into small exogenous siRNAs (exo-siRNAs) . By contrast, when mammalian viruses, such as HIV-1, infect human cells, the production of processed viral exo-siRNAs is rare [19, 35]. Some have interpreted these results to mean that invertebrate cells sense and process viral dsRNAs and then deploy exo-siRNAs in antiviral RISC complexes while vertebrate (mammalian) cells cannot perform these functions. This interpretation however is challenged by experiments in which an authentic short hairpin RNA (shRNA) was engineered into the HIV-1 genome and the genome introduced into human cells, with ensuing efficient siRNA production from the 'viral-shRNA' . Moreover, a version of the above experiment is performed hundreds, if not thousands, of times every day by investigators who use shRNA libraries cloned into lentivirus vectors to transduce human cells for the purpose of silencing specific target genes. In every instance, the lentivirus-shRNA is recognized and processed by human cells faithfully into the expected siRNA (Figure 2).
How then does one reconcile the above observations? One possibility is that human (mammalian) cells, rather than being unable to efficiently recognize and process viral dsRNAs or shRNAs into siRNAs, may actually be more proficient than invertebrate cells at processing and using siRNAs in antiviral RISC complexes against invading viruses. In this case, RNA viruses with double-stranded shRNA-like sequences amenable for processing into siRNAs might be subject to more potent negative selection in human than in invertebrate cells. Over time, strong stringent selection in mammalian cells (and relaxed selection in invertebrate cells) would result in many human viruses being devoid of shRNA-like or dsRNA sequences, while the less robustly restricted invertebrate viruses would still keep shRNA and/or dsRNA sequences. Hence, today's human cells would face many viruses largely lacking shRNA/dsRNA sequences, accounting, in part, for the rarity with which viral exo-siRNAs are detected in mammalian infections (see, for example, ). On the other hand, invertebrate cells would still encounter many viruses that harbor shRNA sequences, and consequently viral exo-siRNAs would more frequently be found in infected invertebrate cells . In short, the argument is that human cells do, in fact, efficiently process viral exo-siRNA and can use siRNA-RISC as an antiviral defense, but the rarity of this process in human cells may be because many mammalian viruses have already been tightly selected by human cells not to maintain siRNA-producing sequences.
They do, however, encode miRNA sequences [38, 39]. These viral miRNAs are processed efficiently in human cells, are engaged in miRNA-RISCs, and are frequently used by the cell (or the virus) to target other viral transcripts. Examples of this type of usage include the human cytomegalovirus (CMV) miR-112-1 targeting the CMV IE1 viral RNA , the EBV BART miRNAs targeting the EBV LMP mRNAs [41, 42], and the HSV miR-H2, miR-H3, and miR-H4 targeting viral ICP0 and ICP34.5 mRNAs . Similar miRNA-mRNA targeting can also occur in the case of a model retroviral infection . Taken together, these findings support the view that mammalian cells employ viral exo-miRNA-RISCs to regulate viral infections, paralleling invertebrate and plant cells that use viral exo-siRNA-RISCs to regulate their cognate viruses (Figure 2).
Outstanding questions and future perspectives
The above arguments apply to retroviruses and herpes viruses, which have been extensively studied for RNAi generation. What remains unaddressed is the processing of the many plus-sense, minus-sense, and human dsRNA viruses. These viruses present fully dsRNA substrates as part of their genomes or as replication intermediates. To date, they have not been extensively investigated for the biogenesis of viral non-coding RNAs, and future findings from these viruses could yield important insights.
Several aspects of the RNAi-virus-host cell interaction also merit closer scrutiny. One issue is whether the expression of endo-siRNAs in mammals is similar between germline tissues and somatic tissues. The data for somatic tissues are currently incomplete. The emerging finding that piRNAs are present in both germ and somatic cells raises the possibility that endo-siRNAs conform to an analogous pattern.
A second issue is the dynamic strike-counterstrike interplay between cells in which RNAi serves to combat viruses and viruses evade RNAi to successfully replicate in cells . One view is that efficiently replicating viruses must encode RNAi suppressors. Indeed, while many mammalian viruses do apparently have RNAi suppressor moieties , an RNAi suppressor function is only one of several means (for example, shielding of the virus genome from RNAi, sequence changes in the viral genome to evade RNAi, virus modulation of cellular miRNA expression, and virus adaptation to cellular RNAi)  at the virus' disposal to skirt cellular RNAi restriction. Indeed, shielding of the viral RNA genome from RNAi , changes in viral sequences to evade RNAi , and virus-modulation of cellular miRNA-expression [48–50] have all been reported for HIV-1. Viruses such as HIV-1 that are highly mutable may evade RNAi efficiently through target sequence changes; these viruses do not need a strong RNAi suppressor [37, 51, 52], if at all . On the other hand, less mutable viruses may require strong RNAi suppressors to mitigate RNAi restriction in order to replicate optimally.
A final issue is the increasingly convincing new evidence for the existence of natural antisense transcripts in human T-lymphotropic virus (HTLV)-1 and HIV-1 [54–58]. Inside cells, these antisense viral RNAs can, in principle, form long RNA duplexes with their complementary sense transcripts. The fate of these dsRNAs and what new functions they may provide promise to keep virologists busy for the next several years.
I thank Christine Kozak, Dong-yan Jin, Andrew Dayton, and Laurent Houzet for advice on this writing and Lauren Lee for preparation of the figures. Work in my laboratory is supported in part by intramural funds from the NIAID and by the IATAP program from the office of the director, NIH. The opinions in this article represent my personal views and do not necessarily reflect the views of my employer, the NIH.
- Kim VN, Han J, Siomi MC: Biogenesis of small RNAs in animals. Nat Rev Mol Cell Biol. 2009, 10: 126-139. 10.1038/nrm2632.PubMedView Article
- Okamura K, Lai EC: Endogenous small interfering RNAs in animals. Nat Rev Mol Cell Biol. 2008, 9: 673-678. 10.1038/nrm2479.PubMedPubMed CentralView Article
- Golden DE, Gerbasi VR, Sontheimer EJ: An inside job for siRNAs. Mol Cell. 2008, 31: 309-312. 10.1016/j.molcel.2008.07.008.PubMedPubMed CentralView Article
- Rother S, Meister G: Small RNAs derived from longer non-coding RNAs. Biochimie. 2011, 93: 1905-1915. 10.1016/j.biochi.2011.07.032.PubMedView Article
- Lee YS, Nakahara K, Pham JW, Kim K, He Z, Sontheimer EJ, Carthew RW: Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways. Cell. 2004, 117: 69-81. 10.1016/S0092-8674(04)00261-2.PubMedView Article
- Tabara H, Yigit E, Siomi H, Mello CC: The dsRNA binding protein RDE-4 interacts with RDE-1, DCR-1, and a DExH-box helicase to direct RNAi in C. elegans. Cell. 2002, 109: 861-871. 10.1016/S0092-8674(02)00793-6.PubMedView Article
- Alvarado V, Scholthof HB: Plant responses against invasive nucleic acids: RNA silencing and its suppression by plant viral pathogens. Semin Cell Dev Biol. 2009, 20: 1032-1040. 10.1016/j.semcdb.2009.06.001.PubMedPubMed CentralView Article
- Brouns SJ, Jore MM, Lundgren M, Westra ER, Slijkhuis RJ, Snijders AP, Dickman MJ, Makarova KS, Koonin EV, van der Oost J: Small CRISPR RNAs guide antiviral defense in prokaryotes. Science. 2008, 321: 960-964. 10.1126/science.1159689.PubMedView Article
- Lander ES, Linton LM, Birren B, Nusbaum C, Zody MC, Baldwin J, Devon K, Dewar K, Doyle M, FitzHugh W, Funke R, Gage D, Harris K, Heaford A, Howland J, Kann L, Lehoczky J, LeVine R, McEwan P, McKernan K, Meldrim J, Mesirov JP, Miranda C, Morris W, Naylor J, Raymond C, Rosetti M, Santos R, Sheridan A, Sougnez C, et al: Initial sequencing and analysis of the human genome. Nature. 2001, 409: 860-921. 10.1038/35057062.PubMedView Article
- Gifford R, Tristem M: The evolution, distribution and diversity of endogenous retroviruses. Virus Genes. 2003, 26: 291-315. 10.1023/A:1024455415443.PubMedView Article
- Kurth R, Bannert N: Beneficial and detrimental effects of human endogenous retroviruses. Int J Cancer. 2010, 126: 306-314. 10.1002/ijc.24902.PubMedView Article
- Deininger PL, Batzer MA: Mammalian retroelements. Genome Res. 2002, 12: 1455-1465. 10.1101/gr.282402.PubMedView Article
- Zhang Y, Maksakova IA, Gagnier L, van de Lagemaat LN, Mager DL: Genome-wide assessments reveal extremely high levels of polymorphism of two active families of mouse endogenous retroviral elements. PLoS Genet. 2008, 4: e1000007-10.1371/journal.pgen.1000007.PubMedPubMed CentralView Article
- Siomi MC, Sato K, Pezic D, Aravin AA: PIWI-interacting small RNAs: the vanguard of genome defence. Nat Rev Mol Cell Biol. 2011, 12: 246-258. 10.1038/nrm3089.PubMedView Article
- Rowe WP, Pincus T: Quantitative studies of naturally occurring murine leukemia virus infection of AKR mice. J Exp Med. 1972, 135: 429-436. 10.1084/jem.135.2.429.PubMedPubMed CentralView Article
- An W, Han JS, Wheelan SJ, Davis ES, Coombes CE, Ye P, Triplett C, Boeke JD: Active retrotransposition by a synthetic L1 element in mice. Proc Natl Acad Sci USA. 2006, 103: 18662-18667. 10.1073/pnas.0605300103.PubMedPubMed CentralView Article
- Yan Z, Hu HY, Jiang X, Maierhofer V, Neb E, He L, Hu Y, Hu H, Li N, Chen W, Khaitovich P: Widespread expression of piRNA-like molecules in somatic tissues. Nucleic Acids Res. 2011, 39: 6596-6607. 10.1093/nar/gkr298.PubMedPubMed CentralView Article
- Rajasethupathy P, Antonov I, Sheridan R, Frey S, Sander C, Tuschl T, Kandel ER: A role for neuronal piRNAs in the epigenetic control of memory-related synaptic plasticity. Cell. 2012, 149: 693-707. 10.1016/j.cell.2012.02.057.PubMedPubMed CentralView Article
- Yeung ML, Bennasser Y, Watashi K, Le SY, Houzet L, Jeang KT: Pyrosequencing of small non-coding RNAs in HIV-1 infected cells: evidence for the processing of a viral-cellular double-stranded RNA hybrid. Nucleic Acids Res. 2009, 37: 6575-6586. 10.1093/nar/gkp707.PubMedPubMed CentralView Article
- Otsuka M, Jing Q, Georgel P, New L, Chen J, Mols J, Kang YJ, Jiang Z, Du X, Cook R, Das SC, Pattnaik AK, Beutler B, Han J: Hypersusceptibility to vesicular stomatitis virus infection in Dicer1-deficient mice is due to impaired miR24 and miR93 expression. Immunity. 2007, 27: 123-134. 10.1016/j.immuni.2007.05.014.PubMedView Article
- Matskevich AA, Moelling K: Dicer is involved in protection against influenza A virus infection. J Gen Virol. 2007, 88: 2627-2635. 10.1099/vir.0.83103-0.PubMedView Article
- Triboulet R, Mari B, Lin YL, Chable-Bessia C, Bennasser Y, Lebrigand K, Cardinaud B, Maurin T, Barbry P, Baillat V, Reynes J, Corbeau P, Jeang KT, Benkirane M: Suppression of microRNA-silencing pathway by HIV-1 during virus replication. Science. 2007, 315: 1579-1582. 10.1126/science.1136319.PubMedView Article
- Chable-Bessia C, Meziane O, Latreille D, Triboulet R, Zamborlini A, Wagschal A, Jacquet JM, Reynes J, Levy Y, Saib A, Bennasser Y, Benkirane M: Suppression of HIV-1 replication by microRNA effectors. Retrovirology. 2009, 6: 26-10.1186/1742-4690-6-26.PubMedPubMed CentralView Article
- Watanabe Y, Kishi A, Yachie N, Kanai A, Tomita M: Computational analysis of microRNA-mediated antiviral defense in humans. FEBS Lett. 2007, 581: 4603-4610. 10.1016/j.febslet.2007.08.049.PubMedView Article
- Jopling CL, Yi MK, Lancaster AM, Lemon SM, Sarnow P: Modulation of hepatitis C virus RNA abundance by a liver-specific microRNA. Science. 2005, 309: 1577-1581. 10.1126/science.1113329.PubMedView Article
- Liu WH, Yeh SH, Chen PJ: Role of microRNAs in hepatitis B virus replication and pathogenesis. Biochim Biophys Acta. 2011, 1809: 678-685.PubMedView Article
- Houzet L, Jeang KT: MicroRNAs and human retroviruses. Biochim Biophys Acta. 2011, 1809: 686-693.PubMedPubMed CentralView Article
- Skalsky RL, Corcoran DL, Gottwein E, Frank CL, Kang D, Hafner M, Nusbaum JD, Feederle R, Delecluse HJ, Luftig MA, Tuschl T, Ohler U, Cullen BR: The viral and cellular microRNA targetome in lymphoblastoid cell lines. PLoS Pathog. 2012, 8: e1002484-10.1371/journal.ppat.1002484.PubMedPubMed CentralView Article
- Riley KJ, Rabinowitz GS, Yario TA, Luna JM, Darnell RB, Steitz JA: EBV and human microRNAs co-target oncogenic and apoptotic viral and human genes during latency. EMBO J. 2012, 31: 2207-2221. 10.1038/emboj.2012.63.PubMedPubMed CentralView Article
- Kang JG, Majerciak V, Uldrick TS, Wang X, Kruhlak M, Yarchoan R, Zheng ZM: Kaposi's sarcoma-associated herpesviral IL-6 and human IL-6 open reading frames contain miRNA binding sites and are subject to cellular miRNA regulation. J Pathol. 2011, 225: 378-389. 10.1002/path.2962.PubMedPubMed CentralView Article
- Wang L, Qin Y, Tong L, Wu S, Wang Q, Jiao Q, Guo Z, Lin L, Wang R, Zhao W, Zhong Z: MiR-342-5p suppresses coxsackievirus B3 biosynthesis by targeting the 2C-coding region. Antiviral Res. 2012, 93: 270-279. 10.1016/j.antiviral.2011.12.004.PubMedView Article
- Zheng ZM, Wang X: Regulation of cellular miRNA expression by human papillomaviruses. Biochim Biophys Acta. 2011, 1809: 668-677.PubMedPubMed CentralView Article
- Russo A, Potenza N: Antiviral effects of human microRNAs and conservation of their target sites. FEBS Lett. 2011, 585: 2551-2555. 10.1016/j.febslet.2011.07.015.PubMedView Article
- Blair CD: Mosquito RNAi is the major innate immune pathway controlling arbovirus infection and transmission. Future Microbiol. 2011, 6: 265-277. 10.2217/fmb.11.11.PubMedPubMed CentralView Article
- Althaus CF, Vongrad V, Niederöst B, Joos B, Di Giallonardo F, Rieder P, Pavlovic J, Trkola A, Günthard HF, Metzner KJ, Fischer M: Tailored enrichment strategy detects low abundant small noncoding RNAs in HIV-1 infected cells. Retrovirology. 2012, 9: 27-10.1186/1742-4690-9-27.PubMedPubMed CentralView Article
- Westerhout EM, Vink M, Haasnoot PC, Das AT, Berkhout B: A conditionally replicating HIV-based vector that stably expresses an antiviral shRNA against HIV-1 replication. Mol Ther. 2006, 14: 268-275. 10.1016/j.ymthe.2006.03.018.PubMedView Article
- Bennasser Y, Le SY, Benkirane M, Jeang KT: Evidence that HIV-1 encodes an siRNA and a suppressor of RNA silencing. Immunity. 2005, 22: 607-619. 10.1016/j.immuni.2005.03.010.PubMedView Article
- Grassmann R, Jeang KT: The roles of microRNAs in mammalian virus infection. Biochim Biophys Acta. 2008, 1779: 706-711.PubMedPubMed CentralView Article
- Kincaid RP, Burke JM, Sullivan CS: RNA virus microRNA that mimics a B-cell oncomiR. Proc Natl Acad Sci USA. 2012, 109: 3077-3082. 10.1073/pnas.1116107109.PubMedPubMed CentralView Article
- Murphy E, Vanicek J, Robins H, Shenk T, Levine AJ: Suppression of immediate-early viral gene expression by herpesvirus-coded microRNAs: implications for latency. Proc Natl Acad Sci USA. 2008, 105: 5453-5458. 10.1073/pnas.0711910105.PubMedPubMed CentralView Article
- Lo AK, To KF, Lo KW, Lung RW, Hui JW, Liao G, Hayward SD: Modulation of LMP1 protein expression by EBV-encoded microRNAs. Proc Natl Acad Sci USA. 2007, 104: 16164-16169. 10.1073/pnas.0702896104.PubMedPubMed CentralView Article
- Lung RW, Tong JH, Sung YM, Leung PS, Ng DC, Chau SL, Chan AW, Ng EK, Lo KW, To KF: Modulation of LMP2A expression by a newly identified Epstein-Barr virus-encoded microRNA miR-BART22. Neoplasia. 2009, 11: 1174-1184.PubMedPubMed CentralView Article
- Jurak I, Griffiths A, Coen DM: Mammalian alphaherpesvirus miRNAs. Biochim Biophys Acta. 2011, 1809: 641-653.PubMedPubMed CentralView Article
- Klase Z, Houzet L, Jeang KT: Replication competent HIV-1 viruses that express intragenomic microRNA reveal discrete RNA-interference mechanisms that affect viral replication. Cell Biosci. 2011, 1: 38-10.1186/2045-3701-1-38.PubMedPubMed CentralView Article
- Haasnoot J, Westerhout EM, Berkhout B: RNA interference against viruses: strike and counterstrike. Nat Biotechnol. 2007, 25: 1435-1443. 10.1038/nbt1369.PubMedView Article
- Westerhout EM, ter BO, Berkhout B: The virion-associated incoming HIV-1 RNA genome is not targeted by RNA interference. Retrovirology. 2006, 3: 57-10.1186/1742-4690-3-57.PubMedPubMed CentralView Article
- Schopman NC, ter BO, Berkhout B: Anticipating and blocking HIV-1 escape by second generation antiviral shRNAs. Retrovirology. 2010, 7: 52-10.1186/1742-4690-7-52.PubMedPubMed CentralView Article
- Houzet L, Yeung ML, de L, Desai D, Smith SM, Jeang KT: MicroRNA profile changes in human immunodeficiency virus type 1 (HIV-1) seropositive individuals. Retrovirology. 2008, 5: 118-10.1186/1742-4690-5-118.PubMedPubMed CentralView Article
- Hayes AM, Qian S, Yu L, Boris-Lawrie K: Tat RNA silencing suppressor activity contributes to perturbation of lymphocyte miRNA by HIV-1. Retrovirology. 2011, 8: 36-10.1186/1742-4690-8-36.PubMedPubMed CentralView Article
- Witwer KW, Watson AK, Blankson JN, Clements JE: Relationships of PBMC microRNA expression, plasma viral load, and CD4+ T-cell count in HIV-1-infected elite suppressors and viremic patients. Retrovirology. 2012, 9: 5-10.1186/1742-4690-9-5.PubMedPubMed CentralView Article
- Qian S, Zhong X, Yu L, Ding B, de HP, Boris-Lawrie K: HIV-1 Tat RNA silencing suppressor activity is conserved across kingdoms and counteracts translational repression of HIV-1. Proc Natl Acad Sci USA. 2009, 106: 605-610. 10.1073/pnas.0806822106.PubMedPubMed CentralView Article
- Haasnoot J, de VW, Geutjes EJ, Prins M, de HP, Berkhout B: The Ebola virus VP35 protein is a suppressor of RNA silencing. PLoS Pathog. 2007, 3: e86-10.1371/journal.ppat.0030086.PubMedPubMed CentralView Article
- Lin J, Cullen BR: Analysis of the interaction of primate retroviruses with the human RNA interference machinery. J Virol. 2007, 81: 12218-12226. 10.1128/JVI.01390-07.PubMedPubMed CentralView Article
- Matsuoka M, Green PL: The HBZ gene, a key player in HTLV-1 pathogenesis. Retrovirology. 2009, 6: 71-10.1186/1742-4690-6-71.PubMedPubMed CentralView Article
- Schopman NC, Willemsen M, Liu YP, Bradley T, van Kampen A, Baas F, Berkhout B, Haasnoot J: Deep sequencing of virus-infected cells reveals HIV-encoded small RNAs. Nucleic Acids Res. 2012, 40: 414-427. 10.1093/nar/gkr719.PubMedPubMed CentralView Article
- Clerc I, Laverdure S, Torresilla C, Landry S, Borel S, Vargas A, Arpin-André C, Gay B, Briant L, Gross A, Barbeau B, Mesnard JM: Polarized expression of the membrane ASP protein derived from HIV-1 antisense transcription in T cells. Retrovirology. 2011, 8: 74-10.1186/1742-4690-8-74.PubMedPubMed CentralView Article
- Lefebvre G, Desfarges S, Uyttebroeck F, Muñoz M, Beerenwinkel N, Rougemont J, Telenti A, Ciuffi A: Analysis of HIV-1 expression level and sense of transcription by high-throughput sequencing of the infected cell. J Virol. 2011, 85: 6205-6211. 10.1128/JVI.00252-11.PubMedPubMed CentralView Article
- Kobayashi-Ishihara M, Yamagishi M, Hara T, Matsuda Y, Takahashi R, Miyake A, Nakano K, Yamochi T, Ishida T, Watanabe T: HIV-1-encoded antisense RNA suppresses viral replication for a prolonged period. Retrovirology. 2012, 9: 38-10.1186/1742-4690-9-38.PubMedPubMed CentralView Article
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