IpsA, a novel LacI-type regulator, is required for inositol-derived lipid formation in Corynebacteria and Mycobacteria
© Baumgart et al.; licensee BioMed Central Ltd. 2013
Received: 13 September 2013
Accepted: 17 December 2013
Published: 30 December 2013
The development of new drugs against tuberculosis and diphtheria is focused on disrupting the biogenesis of the cell wall, the unique architecture of which confers resistance against current therapies. The enzymatic pathways involved in the synthesis of the cell wall by these pathogens are well understood, but the underlying regulatory mechanisms are largely unknown.
Here, we characterize IpsA, a LacI-type transcriptional regulator conserved among Mycobacteria and Corynebacteria that plays a role in the regulation of cell wall biogenesis. IpsA triggers myo-inositol formation by activating ino1, which encodes inositol phosphate synthase. An ipsA deletion mutant of Corynebacterium glutamicum cultured on glucose displayed significantly impaired growth and presented an elongated cell morphology. Further studies revealed the absence of inositol-derived lipids in the cell wall and a complete loss of mycothiol biosynthesis. The phenotype of the C. glutamicum ipsA deletion mutant was complemented to different extend by homologs from Corynebacterium diphtheriae (dip1969) and Mycobacterium tuberculosis (rv3575), indicating the conserved function of IpsA in the pathogenic species. Additional targets of IpsA with putative functions in cell wall biogenesis were identified and IpsA was shown to bind to a conserved palindromic motif within the corresponding promoter regions. Myo-inositol was identified as an effector of IpsA, causing the dissociation of the IpsA-DNA complex in vitro.
This characterization of IpsA function and of its regulon sheds light on the complex transcriptional control of cell wall biogenesis in the mycolata taxon and generates novel targets for drug development.
KeywordsTranscriptional regulator Corynebacterium glutamicum Corynebacterium diphtheriae Mycobacterium tuberculosis Cell wall synthesis Mycolic acids Mycothiol Inositol LM LAM
The human pathogens Mycobacterium tuberculosis and Corynebacterium diphtheriae, as well as many related species of the order Corynebacteriales, share a distinctive cell wall architecture that plays an important role in virulence and imparts effective protection against harsh environmental conditions and resistance to therapies . One unique feature of these cell walls is the presence of long-chain α-alkyl, β-hydroxy fatty acids, called mycolic acids, which form a second bilayer close to the cell surface that is similar to the outer membrane of Gram-negative bacteria . The enzymes and regulatory mechanisms involved in biosynthesis of the cell walls are primary targets for the development of new drugs.
In Mycobacteria, there is a three-step process for synthesis of PI. Step 1 is cyclization of glucose-6-phosphate to 1D-myo-inositol-3-phosphate, a reaction that is catalyzed by a myo-inositol phosphate synthase, Ino1 (Rv0046c) . In step 2, the 1D-myo-inositol-3-phosphate is dephosphorylated by inositol monophosphatase (IMP)  to yield myo-inositol. Step 3 is the transfer of diacylglycerol (DAG) from cytidine diphosphate-diacylglycerol (CDP-DAG) to myo-inositol by a PI synthase, PgsA (Rv2612c) . The enzymes Ino1 and PgsA are essential for the viability of Mycobacteria[10, 11]. An ino1 mutant of M. tuberculosis grows in vitro only in the presence of high inositol concentrations and was no longer able to kill SCID mice (see Movahedzadeh et al., ). In Mycobacterium smegmatis, ino1 has been shown to be essential for growth in the absence of exogenous inositol . Ino1 has therefore been proposed as a target for the development of new antibiotics [10, 13].
In contrast to the wealth of knowledge about the biogenesis of cell walls, little is known about the regulatory circuits that control these processes . IpsA was chosen as target for further studies from a set of mutants strains as a ΔipsA mutant displayed a drastically altered cell morphology hinting towards a function in the control of cell wall biosynthesis. The catabolism of myo-inositol has been examined in C. glutamicum[14, 15] but regulation of myo-inositol formation has not been deeply investigated up until now. Here, a regulator of inositol synthesis in the model organism C. glutamicum is described. Named IpsA, it is a novel LacI-type regulator that acts as an inositol-dependent transcriptional activator of the myo-inositol phosphate synthase gene. IpsA function is conserved throughout Corynebacteria and Mycobacteria, including the prominent pathogenic species C. diphtheriae and M. tuberculosis.
IpsA is a novel LacI-type regulator conserved in the Corynebacteriales
IpsA was assigned by homology to the LacI family of transcriptional regulators, which often coordinate the available nutrients with the expression of catabolic genes . The corresponding genomic loci display a remarkable level of conservation across the Corynebacteriales (Figure 1B). In previous studies, the structure of IpsA (Cg2910) was analyzed (PDB:3H5T); its physiological function is the subject of this study.
IpsA deletion affects cell shape and growth
To gain insight into the function and possible target genes of IpsA, an in-frame deletion mutant was constructed. Growth rates and final backscatter of ΔipsA cells were significantly below control levels using minimal medium with glucose as carbon source, and these shortfalls increased in magnitude with successive subcultivation (Figure 1C). The morphology of the mutant strain was altered, as revealed by fluorescence microscopy with staining of DNA (4’,6-diamidino-2-phenylindole (DAPI)) and membranes (nile red). ΔipsA cells formed chain-like structures and failed to divide properly. DNA in the cells was unevenly distributed and was frequently found in the extracellular space, indicative of severe damage to the cell membrane and/or the cell wall (Figure 1D, for a larger sample see Additional file 1: Figure S1). Membrane staining revealed several intact cell septa in the unshaped mutant cells.
The impact of ipsAmutation on transcription
To elucidate the transcriptional changes caused by the deletion of ipsA, DNA microarray experiments were performed. More than 450 genes showed alterations in mRNA level greater than a factor of 2, using cells in the exponential growth phase (OD600 = 5) cultivated in minimal medium with glucose as carbon source (Additional file 1: Table S3, full data deposited in GEO database  under accession number GSE50210).
Transcriptome analysis of an IpsA deletion mutant
Myo-inositol phosphate synthase
ABC transporter/periplasmic d-ribose-binding protein
Probable ABC transport protein, membrane component
Transcriptional regulator, LacI family
Probable ABC transport protein, ATP-binding component
Cell envelope-related transcriptional regulator
Putative dinucleotide-binding enzyme
O-succinylbenzoic acid-CoA ligase
Putative integral membrane protein
Identification of direct IpsA target promoters
Determination of the IpsA DNA binding site
Myo-inositol is an effector molecule of IpsA
The enzyme encoded by the main IpsA target gene is Ino1, a myo-inositol phosphate synthase that catalyzes the formation of 1D-myo-inositol-3-phosphate from glucose-6-phosphate . We tested several metabolites involved in the relevant pathway for their influence on the formation of the IpsA-DNA complex. A clear backshift was observed for myo-inositol but not for 1D-myo-inositol-1-phosphate, 1D-myo-inositol-3-phosphate or glucose-6-phosphate (Figure 3B). The effect of myo-inositol could be observed at concentrations down to 0.2 mM (Additional file 1: Figure S6). This effect is specific for IpsA DNA binding, as the addition of up to 50 mM myo-inositol did not have any effect on the formation of an unrelated protein-DNA complex (for example, AcnR and the acn promoter, Additional file 1: Figure S7).
IpsA is required for inositol-dependent regulation of ino1
The major function of IpsA revealed thus far is the activation of ino1 in the absence of external inositol. Consistent with this, the growth and cell morphology of the ΔipsA strain is complemented by the constitutive expression of ino1 under standard conditions (Figure 2C). To study the influence of IpsA and myo-inositol on the expression of ino1 in vivo, we fused the ino1 promoter to eyfp (enhanced yellow fluorescent protein) and monitored the fluorescence output of the wild-type and the ΔipsA strain during growth on glucose and myo-inositol (Figure 3D). Strong promoter activity was observed in the wild-type during growth on glucose. Expression of eyfp was significantly decreased in the ipsA deletion strain and was lower still when either strain was cultivated with myo-inositol as the carbon source.
IpsA function is conserved in C. diphtheriae and M. tuberculosis
We also tested the binding of IpsA to the promoter regions of C. diphtheriae and M. tuberculosis homologs of cg3323 and cg0044 using EMSAs (Figure 5D). C. glutamicum IpsA bound strongly to the C. diphtheriae promoters DIP0115 and DIP0021 and the binding sites were determined (Figure 5E and Additional file 1: Figure S8). In M. tuberculosis, Rv0046c is organized in an operon with Rv0047c. A binding site was identified within the open reading frame (ORF) of Rv0047c, suggesting the occurrence of a second promoter upstream of ino1 (Figure 5E).
Mycothiol synthesis is abolished in C. glutamicum ∆ipsA
PI-based glycolipid synthesis is lost in C. glutamicum ∆ipsA
Analysis of lipoglycan extracts
Since the lipoglycans LM and LAM are built on PIMs which are mannosylated products of PI, the effect of IpsA was also studied on these lipoglycans extracted from the wild-type, C. glutamicum ∆ipsA and the complemented strains (Additional file 1: Figure S10A). LAM and Cg-LM-A/B were clearly present in extracts from the wild-type cultured in glucose or myo-inositol. However, the lipoglycan extracts from the mutant C. glutamicum ∆ipsA grown on glucose revealed no LAM while a band corresponding to Cg-LM-B could be observed. On glucose the complemented strains C. glutamicum ΔipsA::pK18-ipsA and C. glutamicum ΔipsA containing plasmid encoded Cg3323 and DIP1969 exhibited lipoglycan profiles identical to the wild-type as distinct bands corresponding to both LAM and Cg-LM-A/B. However, no band corresponding to LAM was observed in lipoglycan extracts of C. glutamicum ΔipsA complemented with Rv3575c in pAN6 plasmid grown in minimal media supplemented with glucose. Instead, a band corresponding to Cg-LM-B was evident. The lipoglycan extracts isolated from the mutant and complemented strains cultured in minimal media supplemented with myo-inositol were similar to wild-type with distinct LM and LAM (Additional file 1: Figure S10B).
The polyol myo-inositol has an important function in Mycobacteria and Corynebacteria: it serves as the key building block for a large class of cell wall phospholipids, as well as for the antioxidant MSH [1, 3]. Several studies have illustrated the potential of the underlying biosynthetic pathways as putative drug targets . For example, an ino1 mutant of M. tuberculosis can only grow in the presence of high concentrations of myo-inositol  and a M. tuberculosis mutant defective in MSH biosynthesis requires catalase for growth on plates . Our studies have shown that the deletion of ipsA leads to a complete loss of mycothiol biosynthesis, demonstrating the importance of IpsA also for this metabolic pathway.
Myo-inositol and its phosphate derivatives are common substances in eukaryotes and are therefore present in significant quantities in the natural habitats of M. tuberculosis, C. diphtheriae (the human body) and C. glutamicum (soil) . It has been shown that myo-inositol can be used by C. glutamicum as a carbon and energy source, and several transporters, enzymes and regulators of the catabolic pathway have been identified [14, 15]. In the present study, microarray analysis revealed that the two main target genes of IpsA, ino1 and cg0044, are strongly downregulated during growth on inositol compared to growth on glucose, which is in line with IpsA being an inositol-dependent activator of both genes/operons .
Bioinformatic analysis of ipsA and its homologs in other Corynebacteria and Mycobacteria revealed conservation of the gene among these species (Figure 1B). In many genomes of the Corynebacteriales, ipsA is located next to znuACB, which encodes a putative zinc transport system regulated by Zur . Furthermore, genes involved in the biosynthesis of trehalose (otsAB), which is also an important component of the cell wall of the Corynebacteriales, are frequently located downstream of ipsA, but their orientations are divergent [28, 29]. The product of the main target gene of IpsA, Ino1, contains a zinc ion in its catalytic center and needs NAD+ for its reaction .
The transcriptional organization of ino1 differs between Corynebacteria and Mycobacteria. While ino1 is the only gene in the transcriptional unit in Corynebacteria, it is cotranscribed with a preceded, uncharacterized PadR-like transcriptional regulator in Mycobacteria (Additional file 1: Figure S11). Given the genomic organization, we suspect that this regulator is an additional player in the control of ino1 expression.
That ino1 is the main target gene of IpsA is supported by the observation that the ipsA phenotype was complemented by overexpression of ino1. One could assume that this is the only real target gene of IpsA, but it is not unlikely, that other target genes only appear to be important under different conditions, for example, another carbon source or certain stress conditions. Based on DNA Microarrays and EMSAs we identified several other direct target genes of IpsA, all of which were previously uncharacterized. The operon cg0044 to cg0046 encodes an ABC transporter of unknown function. It may be involved in the uptake of inositol(-derived) carbohydrates required for cell wall biogenesis. The product of cg3210 is annotated as a cell envelope-related transcriptional regulator of the LytR type. It has a transmembrane domain and is conserved in Corynebacteria and Mycobacteria. Cg1421 is a putative dinucleotide-binding enzyme that has some similarity with coenzyme F420-dependent NADP oxidoreductases. Cg3195 is annotated as a flavin-containing monooxygenase and has, like Cg1421, a NAD(P) binding domain. As many MSH-dependent detoxification reactions require NAD or NADP as cofactors , Cg1421 and Cg3195 may be involved in cofactor supply or regeneration for these processes. The operon cg0534 to cg0533 encodes a putative integral membrane protein of the YphA family and menE, an O-succinylbenzoic acid-CoA ligase. Although the functions of the target genes besides ino1 are largely unknown, there are some clear links to cell wall synthesis and regulation, and likely also to MSH-associated reactions. Thus, our analysis of the IpsA regulon has revealed several novel and interesting targets for further studies.
Among the target genes of IpsA are not only activated but also repressed genes. The localization of the IpsA binding site in the particular promoter regions matches very well with the type of regulation (Additional file 1: Figure S5). For the activated genes (cg3323, cg0044, cg3210, and cg3195) IpsA binding sites are located upstream of the -35 region. In the promoters of the repressed genes (cg1421 and cg0534) the IpsA binding sites overlap with the -35 and/or the -10 region. This is a further indication that the altered mRNA level we see in the DNA microarrays is a direct effect of the IpsA deletion.
The structure-function relationship of IpsA is comparable to other members of the LacI family of transcriptional regulators. They all contain a DNA-binding domain at the N-terminus of the protein, composed of three α-helices that form a helix-turn-helix motif that binds to the major groove of the operator. The tetramerization of LacI takes place at the C-terminus, at residues 340 to 357, (Additional file 1: Figure S12) . These residues are missing in IpsA, which is in agreement with our gel filtration analysis that indicated a dimeric conformation for IpsA. A computational analysis of many LacI regulators and binding sites revealed a highly conserved hydrophobic amino acid at position 54 (mostly leucine, L64 in IpsA), which inserts into a conserved, central CG group in the operator. This group is present in both binding motifs of the ino1 promoter in C. glutamicum.
The formation of 1D-myo-inositol-3-phosphate from glucose-6-phosphate by Ino1 represents the first step of PI synthesis; it provides the scaffold on which cell wall components such as PIMs, LM and LAM are built. Our chromatographic analysis of radiolabelled polar lipid extracts of the mutant C. glutamicum ∆ipsA grown in minimal media with glucose generated a characteristic phenotype, namely that the mutant strain was deficient in AcPIM2 and PI while Gl-A and Gl-X were unaffected. In addition, the mutant strain grown on minimal media supplemented with glucose failed to produce PI-based LAM. Instead, the GL-X-based LM, Cg-LM-B was apparent on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of lipoglycans, which could explain why C. glutamicum ∆ipsA can grow over several cultivations without supplementation with myo-inositol . This LM-B was not found in Mycobacteria. The polar lipid and lipoglycan extracts from the complemented strain C. glutamicum ∆ipsA::pK18-ipsA grown on minimal media supplemented with glucose displayed normal levels of AcPIM2, PI, LM and LAM biosynthesis, with profiles identical to wild-type. These data are consistent with the view that IpsA affects an early stage of PI biosynthesis, namely the expression of ino1. In fact, the polar lipid and lipoglycan extracts obtained from ΔipsA complemented with plasmid-encoded ino1 also displayed lipid profiles identical to that of the wild-type, indicating involvement of IpsA in transcriptional activation of ino1 expression. The results imply that in absence of IpsA, PI-based PIMs, LM and LAM are not synthesized as the mutant strain no longer produces the inositol monophosphate synthase required for synthesis of myo-inositol, a substrate for PI production. The polar lipid extracts of C. glutamicum ∆ipsA complemented with the homolog from C. diphtheriae exhibited a profile akin to wild-type. Thus DIP1969 is a functional equivalent of IpsA and can be designated as transcriptional activator of ino1 (DIP0115) in this pathogenic species. For the homolog of Rv3575 the situation is not as clear as for DIP1969. Although we see a positive effect on growth and a partial complementation in terms of mycothiol biosynthesis and morphological phenotype, no PI derived lipids could be identified on the TLC results. One possible explanation for this is that most of the inositol formed is channeled towards mycothiol. Consequently, a low amount of PI derived lipids might be formed, sufficient for partial complementation of the phenotype but still too low for detection by TLC. Furthermore, the differences in M. tuberculosis IpsA structure, binding affinity and specificity can also be a reason for the only partial complementation. The fact that we did observe partial complementation with respect to most phenotypes tested hints at least to a very similar function in M. tuberculosis.
In this study, we have characterized the LacI regulator IpsA as a first regulator of inositol anabolism in the Corynebacteriales. The findings provide insights into the regulatory mechanisms involved in processes that have been identified as promising targets for drug development. IpsA itself could be such a target, if a myo-inositol analog could be developed that blocks activation of ino1; it is noteworthy that a trehalose analog with a bacteriostatic effect on Mycobacterium aurum has been reported . Finally, the IpsA regulon delivers a promising starting point for the analysis of further, so far unstudied players in cell wall synthesis in this important class of bacteria.
Bacterial strains, plasmids and growth media
The bacterial strains and plasmids used in this study are listed in Additional file 1: Table S1. The C. glutamicum type strain ATCC 13032 was used as wild-type. Growth experiments were performed at 30°C and 1,200 rpm in a Biolector system (m2p-labs, Baesweiler, Germany) in 48-well FlowerPlates containing 750 μL CGXII minimal medium  supplemented with 3,4-dihydroxybenzoate (30 mg/L) and carbon source as indicated. If appropriate, 25 μg/mL kanamycin was added. All cloning was performed in Escherichia coli DH5α cultivated at 37°C in lysogeny broth (LB, ) with 50 μg/L kanamycin.
Recombinant DNA work
Routine methods such as polymerase chain reaction (PCR), DNA restriction and ligation were performed using standard protocols [36–38]. The oligonucleotides used in this study were obtained from Eurofins MWG Operon (Ebersberg, Germany) and are listed in Additional file 1: Table S2. DNA sequencing was performed by Eurofins MWG Operon (Ebersberg, Germany). The ΔipsA mutant of C. glutamicum was constructed via a two-step homologous recombination protocol as described previously . For further details regarding plasmid and mutant construction, see Additional file 1.
For transcriptome analysis, C. glutamicum wild-type and ΔipsA cells were grown in 5 mL BHI (brain-heart infusion, Becton, Dickinson and Company, Le Pont de Claix, France) for about 6 h at 30°C. A second precultivation was performed in CGXII minimal medium containing 2% (w/v) glucose as carbon source. The main cultures were inoculated to an OD of 0.5 in CGXII minimal medium with 2% (w/v) glucose. At an OD of 5 the cells were harvested by centrifugation (4,120 g, 10 minutes and 4°C). The cell pellet was subsequently frozen in liquid nitrogen and stored at -70°C. The preparation of total RNA was performed as described previously with the RNeasy Kit from Qiagen (Hilden, Germany) . Synthesis of fluorescently-labeled cDNA was carried out as described in [40, 41]. Purified cDNA samples to be compared were pooled and the prepared two-color samples were hybridized at 65°C while rotating for 17 h using Agilent’s Gene Expression Hybridization Kit (Agilent, Böbligen, Germany), hybridization oven and hybridization chamber. After hybridization, the arrays were washed using Agilent’s Wash Buffer Kit according to the manufacturer’s instructions. Fluorescence of hybridized DNA microarrays was determined at 532 nm (Cy3) and 635 nm (Cy5) at 5 μm resolution with a GenePix 4000B laser scanner and GenePix Pro 6.0 software (Molecular Devices, Sunnyvale, CA, USA). Fluorescence images were saved to raw data files in TIFF format (GenePix Pro 6.0). Quantitative TIFF image analysis was carried out using GenePix image analysis software and results were saved as GPR-file (GenePix Pro 6.0). For background correction of spot intensities, ratio calculation and ratio normalization, GPR-files were processed using the BioConductor R-packages limma and marray . Array data were deposited in the GEO database  under accession number GSE50210.
Overproduction and purification of IpsA
E. coli BL21(DE3) carrying the expression plasmids pET-TEV-ipsA or pAN6-ipsA-STREP were grown in LB medium at 37°C and 120 rpm. IpsA overproduction was induced by addition of 50 μM isopropyl β-d-1-thiogalactopyranoside (IPTG) followed by cultivation at 16°C for 20 h before the cells were harvested by centrifugation. Nickel chelate and StrepTactin affinity chromatography were performed as described previously [44, 45]. For further purification and determination of the molecular weight, gel filtration was performed using a Superdex™ 200 10/300 GL column (GE Healthcare, Munich, Germany) (buffer: 50 mM Tris-HCl pH 8, 250 mM NaCl, 1 mM dithiothreitol (DTT)). Note that the IpsA-variant with the C-terminal STREP-tag formed less aggregates than the variant with the His10-tag and eluted at a volume corresponding to 79 kDa, suggesting dimer formation (Additional file 1: Figure S2). The protein was concentrated, flash frozen in liquid nitrogen and stored in the gel filtration buffer at -70°C.
Electrophoretic mobility shift assays (EMSAs)
EMSAs were performed as described previously with the following modifications . Unless otherwise stated, the STREP-tag variant was used for the EMSAs. Purified IpsA was incubated with DNA fragments (30 to 500 bp, final concentration 0.028 to 1 μM) in binding buffer (50 mM Tris-HCl pH 7.5, 40 mM KCl, 5 mM MgCl2) in a total volume of 10 μL. Electrophoresis was performed with 10% to 15% native polyacrylamide gels at room temperature and 150 or 180 V for 45 to 60 minutes (depending on the size of the DNA fragments). For the testing of effector molecules, IpsA was first incubated with the putative effector for 20 minutes at room temperature, followed by addition of the DNA, further 20 minutes incubation and electrophoresis as described above. As control AcnR protein and the acn promoter fragment was used as described in  (Additional file 1: Figure S7).
For recording fluorescence microscopy images, cells were centrifuged and resuspended in phosphate-buffered saline (PBS, 137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4, 1.4 mM KH2PO4, pH 7.3) containing 200 ng/mL DAPI and 300 ng/mL nile red. After 5 to 10 minutes incubation at room temperature, the cells were analyzed on agar pads using a Zeiss Axioplan 2 imaging microscope that was equipped with an AxioCam MRm camera and a Plan-Apochromat 100×, 1.40 Oil DIC oil-immersion objective. Digital images were acquired and analyzed with AxioVision 4.6 software (Zeiss, Göttingen, Germany).
Promoter fusion studies
To analyze the regulation of the ino1 promoter by IpsA in vivo, a DNA fragment covering the cg3323 promoter region was fused to the eyfp-coding sequence (pJC1-Pcg3323-eYFP). Wild-type and ΔipsA cells were transformed with the resulting plasmid and the fluorescence output was analyzed on minimal medium with 2% (w/v) glucose or myo-inositol in the Biolector system.
Extraction and determination of mycothiol
The determination of mycothiol was carried out as described . Briefly, cells were first cultivated in BHI medium for 6 h and then twice in CGXII minimal medium with 4% (w/v) as carbon source at 30°C and 120 rpm for 16 h and 24 h, respectively. 200 mg of wet cells were resuspended in 1 mL of warm (60°C) acetonitrile containing 20 mM Tris-HCl, pH 8.0 and 2 mM bromobimane (Sigma-Aldrich, Taufkirchen near Munich, Germany), sonificated for 20 s and further incubated in a 60°C water bath for 15 minutes in the dark. The samples were acidified with 5 μL of 5 M methanesulfonic acid and the cellular debris was removed by centrifugation at 16,000 g for 10 minutes. The supernatants were filtered and diluted fivefold in 10 mM methanesulfonic acid. A wild-type control sample was incubated with 5 mM NMM to block the thiol groups prior to derivatization with bromobimane. The sample were analyzed using a C18 column (LiChrospher RP 18, 125 × 4 mm) and the solvents A (100 mM sodium acetate buffer pH 7.2) and B (100% methanol) a flow rate of 0.7 mL/minute and the following gradient: 0 minutes, 15% B; 1 minute, 15% B; 1.5 minutes, 35% B; 11 minutes, 80% B; 12 minutes, 95% B; 13 minutes 100% B. The peak assumed to be mycothiol (5.61 minutes) was identified by comparison of the wild-type chromatogram with the one from the ΔmshC strain and the NMM treated sample. Furthermore this peak appears shortly after glutathione (5.14) as published for a similar method .
Growth of bacteria for lipid and lipoglycan analysis
Overnight cultures of C. glutamicum ATCC 13032, C. glutamicum ΔipsA, C. glutamicum ΔipsA::pK18int-ipsA, and C. glutamicum ΔipsA harboring ino1 (cg3323), Rv3575 and DIP1969 in plasmid pAN6 were pregrown in 20 mL of 3.7% brain-heart-glucose bouillon (Sigma-Aldrich, Gillingham, UK) containing Kanamycin (25 μg/mL). The cells were washed once with PBS, used to inoculate a second preculture to an OD600 of 1 in 50 mL CGXII minimal medium (50 mL) supplemented with 2% glucose and incubated with gentle shaking overnight at 30°C. The cells of the second preculture were washed once with PBS and used to inoculate the main cultures to an OD600 of 0.1 in 10 mL CGXII minimal medium (10 mL), supplemented with either 2% glucose or 2% myo-inositol and grown until early log phase. At early log phase, the cells were labeled with 1 μCi/mL [1,2-14C]-acetate (1.66 to 2.22GBq/mmol, PerkinElmer Inc., Waltham, Massachusetts, USA) and incubated overnight at 30°C with gentle shaking. The cells were recovered following centrifugation at 3,500 g and dried.
Analysis of polar lipids and lipoglycans
The cell cultivation procedure for the following experiments is described in the Additional file 1. Polar and apolar lipid extracts were prepared from the dried cell pellets using established procedures . The dried polar lipid extracts were resuspended in CHCl3:CH3OH (100 μL, 2: 1, v/v) and the incorporation of [1,2-14C] acetate in lipid extracts determined by counting an aliquot (5%) using scintillation fluid (5 mL). To analyze the lipid profiles, equal counts of polar lipid extracts (20,000 cpm) were applied to silica thin layer chromatography (silica gel 60 F254 Merck 5554, TLC plates) and developed using the solvent system: CHCl3:CH3OH:H2O (60:30:6, v/v/v) in the first dimension and CHCl3:CH3CO2H:CH3OH:H2O (40:25:3:6, v/v/v/v) in the second dimension. The autoradiograms were obtained by exposing the TLC results to X-ray films (Kodak-Biomax MR Kodak, Rochester, New York, USA) for 48 h.
Lipoglycans were extracted using previously described protocols . Briefly, dried cell pellets from a 10 mL volume culture were resuspended in water and refluxed five times with equal volume of 50% aqueous C2H5OH at 85°C, for 6 h intervals. The suspension was centrifuged and the supernatant recovered between each extraction following centrifugation. The combined supernatant fractions were dried and subjected to hot phenol-H2O treatment at 65°C. The aqueous phase containing the crude lipoglycan fraction was recovered and dialyzed (molecular weight cut-off 3.5 KDa) against water. The dialyzed fraction was dried and 20 μg of lipoglycans loaded on a 15% SDS-PAGE gel. The lipoglycans were visualized using Pro-Q emerald glycoprotein stain (Life Technologies, Darmstadt, Germany).
enhanced yellow fluorescent protein
This work was supported by the German Ministry of Education and Research (BMBF, grant 0316017B) and by the Helmholtz Association (Young Investigator grant VH-NG-716). We thank Tino Polen for help with the microarray analyses, Jan van Ooyen for help with high-performance liquid chromatography (HPLC) measurements, the students Jakob Schlegel and Sebastian Schnorrenberg for their contribution to this project, Antonia Heyer for providing plasmid pJC1-venus-term, Jörn Kalinowski for information about transcriptional start sites as well as Lothar Eggeling and Simon Klaffl for discussion.
- Mishra AK, Driessen NN, Appelmelk BJ, Besra GS: Lipoarabinomannan and related glycoconjugates: structure, biogenesis and role in Mycobacterium tuberculosis physiology and host-pathogen interaction. FEMS Microbiol Rev. 2011, 35: 1126-1157. 10.1111/j.1574-6976.2011.00276.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Eggeling L, Besra GS, Alderwick L: Structure and synthesis of the cell wall. Corynebacteria: genomics and molecular biology. Edited by: Burkovski A. 2008, Wymondham, UK: Caister Academic Press, 267-294.Google Scholar
- Fahey RC: Glutathione analogs in prokaryotes. Biochim Biophys Acta. 1830, 2013: 3182-3198.Google Scholar
- Morita YS, Fukuda T, Sena CBC, Yamaryo-Botte Y, McConville MJ, Kinoshita T: Inositol lipid metabolism in mycobacteria: biosynthesis and regulatory mechanisms. Biochim Biophys Acta. 1810, 2011: 630-641.Google Scholar
- Alm EJ, Huang KH, Price MN, Koche RP, Keller K, Dubchak IL, Arkin AP: The MicrobesOnline web site for comparative genomics. Genome Res. 2005, 15: 1015-1022. 10.1101/gr.3844805.PubMed CentralView ArticlePubMedGoogle Scholar
- Bachhawat N, Mande SC: Identification of the INO1 gene of Mycobacterium tuberculosis H37Rv reveals a novel class of inositol-1-phosphate synthase enzyme. J Mol Biol. 1999, 291: 531-536. 10.1006/jmbi.1999.2980.View ArticlePubMedGoogle Scholar
- Nigou J, Besra GS: Characterization and regulation of inositol monophosphatase activity in Mycobacterium smegmatis. Biochem J. 2002, 361: 385-390.PubMed CentralView ArticlePubMedGoogle Scholar
- Movahedzadeh F, Wheeler PR, Dinadayala P, Av-Gay Y, Parish T, Daffe M, Stoker NG: Inositol monophosphate phosphatase genes of Mycobacterium tuberculosis. BMC Microbiol. 2010, 10: 50-10.1186/1471-2180-10-50.PubMed CentralView ArticlePubMedGoogle Scholar
- Movahedzadeh F, Smith DA, Norman RA, Dinadayala P, Murray-Rust J, Russell DG, Kendall SL, Rison SC, McAlister MS, Bancroft GJ, McDonald NQ, Daffe M, Av-Gay Y, Stoker NG: The Mycobacterium tuberculosis ino1 gene is essential for growth and virulence. Mol Microbiol. 2004, 51: 1003-1014. 10.1046/j.1365-2958.2003.03900.x.View ArticlePubMedGoogle Scholar
- Jackson M, Crick DC, Brennan PJ: Phosphatidylinositol is an essential phospholipid of mycobacteria. J Biol Chem. 2000, 275: 30092-30099.View ArticlePubMedGoogle Scholar
- Haites RE, Morita YS, McConville MJ, Jacobe HB: Function of phosphatidylinositol in mycobacteria. J Biol Chem. 2005, 280: 10981-10987. 10.1074/jbc.M413443200.View ArticlePubMedGoogle Scholar
- Hernick M: Mycothiol: a target for potentiation of rifampin and other antibiotics against Mycobacterium tuberculosis. Expert Rev Anti Infect Ther. 2013, 11: 49-67. 10.1586/eri.12.152.View ArticlePubMedGoogle Scholar
- Krings E, Krumbach K, Bathe B, Kelle R, Wendisch VF, Sahm H, Eggeling L: Characterization of myo-inositol utilization by Corynebacterium glutamicum: the stimulon, identification of transporters, and influence on L-lysine formation. J Bacteriol. 2006, 188: 8054-8061. 10.1128/JB.00935-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Klaffl S, Brocker M, Kalinowski J, Eikmanns B, Bott M: Complex regulation of the PEP carboxykinase gene pck and characterization of its GntR-type regulator IolR as a repressor of myo-inositol utilization genes in Corynebacterium glutamicum. J Bacteriol. 2013, 195: 4283-4296. 10.1128/JB.00265-13.PubMed CentralView ArticlePubMedGoogle Scholar
- Swint-Kruse L, Matthews KS: Allostery in the Lacl/GaIR family: variations on a theme. Curr Opin Microbiol. 2009, 12: 129-137. 10.1016/j.mib.2009.01.009.PubMed CentralView ArticlePubMedGoogle Scholar
- Wennerhold J, Bott M: The DtxR regulon of Corynebacterium glutamicum. J Bacteriol. 2006, 188: 2907-2918. 10.1128/JB.188.8.2907-2918.2006.PubMed CentralView ArticlePubMedGoogle Scholar
- Wennerhold J, Krug A, Bott M: The AraC-type regulator RipA represses aconitase and other iron proteins from Corynebacterium under iron limitation and is itself repressed by DtxR. J Biol Chem. 2005, 280: 40500-40508. 10.1074/jbc.M508693200.View ArticlePubMedGoogle Scholar
- Bailey TL, Elkan C: “Fitting a mixture model by expectation maximization to discover motifs in biopolymers”, Proceedings of the Second International Conference on Intelligent Systems for Molecular Biology. 1994, Menlo Park, Ca: AAAI Press, 28-36.Google Scholar
- Feng J, Che YS, Milse J, Yin YJ, Liu L, Rückert C, Shen XH, Qi SW, Kalinowski J, Liu SJ: The gene ncgl2918 encodes a novel maleylpyruvate isomerase that needs mycothiol as cofactor and links mycothiol biosynthesis and gentisate assimilation in Corynebacterium glutamicum. J Biol Chem. 2006, 281: 10778-10785. 10.1074/jbc.M513192200.View ArticlePubMedGoogle Scholar
- Tatituri RV, Illarionov PA, Dover LG, Nigou J, Gilleron M, Hitchen P, Krumbach K, Morris HR, Spencer N, Dell A, Eggeling L, Besra GS: Inactivation of Corynebacterium glutamicum NCgl0452 and the role of MgtA in the biosynthesis of a novel mannosylated glycolipid involved in lipomannan biosynthesis. J Biol Chem. 2007, 282: 4561-4572.View ArticlePubMedGoogle Scholar
- Mishra AK, Klein C, Gurcha SS, Alderwick LJ, Babu P, Hitchen PG, Morris HR, Dell A, Besra GS, Eggeling L: Structural characterization and functional properties of a novel lipomannan variant isolated from a Corynebacterium glutamicum pimB′ mutant. Antonie Van Leeuwenhoek. 2008, 94: 277-287. 10.1007/s10482-008-9243-1.PubMed CentralView ArticlePubMedGoogle Scholar
- Mishra AK, Batt S, Krumbach K, Eggeling L, Besra GS: Characterization of the Corynebacterium glutamicum ΔpimB′ ΔmgtA double deletion mutant and the role of Mycobacterium tuberculosis orthologues Rv2188c and Rv0557 in glycolipid biosynthesis. J Bacteriol. 2009, 191: 4465-4472. 10.1128/JB.01729-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Vilchèze C, Av-Gay Y, Attarian R, Liu Z, Hazbón MH, Colangeli R, Chen B, Liu W, Alland D, Sacchettini JC, Jacobs WR: Mycothiol biosynthesis is essential for ethionamide susceptibility in Mycobacterium tuberculosis. Mol Microbiol. 2008, 69: 1316-1329. 10.1111/j.1365-2958.2008.06365.x.PubMed CentralView ArticlePubMedGoogle Scholar
- Turner BL, Paphazy MJ, Haygarth PM, McKelvie ID: Inositol phosphates in the environment. Philos T Roy Soc B. 2002, 357: 449-469. 10.1098/rstb.2001.0837.View ArticleGoogle Scholar
- Schröder J, Jochmann N, Rodionov DA, Tauch A: The Zur regulon of Corynebacterium glutamicum ATCC 13032. BMC Genomics. 2010, 11: 12-10.1186/1471-2164-11-12.PubMed CentralView ArticlePubMedGoogle Scholar
- Tropis M, Meniche X, Wolf A, Gebhardt H, Strelkov S, Chami M, Schomburg D, Kramer R, Morbach S, Daffe M: The crucial role of trehalose and structurally related oligosaccharides in the biosynthesis and transfer of mycolic acids in corynebacterineae. J Biol Chem. 2005, 280: 26573-26585. 10.1074/jbc.M502104200.View ArticlePubMedGoogle Scholar
- Murphy HN, Stewart GR, Mischenko VV, Apt AS, Harris R, McAlister MSB, Driscoll PC, Young DB, Robertson BD: The OtsAB pathway is essential for trehalose biosynthesis in Mycobacterium tuberculosis. J Biol Chem. 2005, 280: 14524-14529. 10.1074/jbc.M414232200.View ArticlePubMedGoogle Scholar
- Norman RA, McAlister MSB, Murray-Rust J, Movahedzadeh F, Stoker NG, McDonald NQ: Crystal structure of inositol 1-phosphate synthase from Mycobacterium tuberculosis, a key enzyme in phosphatidylinositol synthesis. Structure. 2002, 10: 393-402. 10.1016/S0969-2126(02)00718-9.View ArticlePubMedGoogle Scholar
- Roback P, Beard J, Baumann D, Gille C, Henry K, Krohn S, Wiste H, Voskuil MI, Rainville C, Rutherford R: A predicted operon map for Mycobacterium tuberculosis. Nucleic Acids Res. 2007, 35: 5085-5095. 10.1093/nar/gkm518.PubMed CentralView ArticlePubMedGoogle Scholar
- Bell CE, Lewis M: The Lac repressor: a second generation of structural and functional studies. Curr Opin Struct Biol. 2001, 11: 19-25. 10.1016/S0959-440X(00)00180-9.View ArticlePubMedGoogle Scholar
- Camas FM, Alm EJ, Poyatos JF: Local gene regulation details a recognition code within the LacI transcriptional factor family. PLoS Comput Biol. 2010, 6: 11-View ArticleGoogle Scholar
- Belisle JT, Vissa VD, Sievert T, Takayama K, Brennan PJ, Besra GS: Role of the major antigen of Mycobacterium tuberculosis in cell wall biogenesis. Science. 1997, 276: 1420-1422. 10.1126/science.276.5317.1420.View ArticlePubMedGoogle Scholar
- Keilhauer C, Eggeling L, Sahm H: Isoleucine synthesis in Corynebacterium glutamicum - Molecular analysis of the Ilvb-Ilvn-Ilvc Operon. J Bacteriol. 1993, 175: 5595-5603.PubMed CentralPubMedGoogle Scholar
- Sambrook J, Russell DW: Molecular cloning: A Laboratory Manual. 2001, Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press, 3Google Scholar
- Hanahan D: Studies on transformation of Escherichia coli with plasmids. J Mol Biol. 1983, 166: 557-580. 10.1016/S0022-2836(83)80284-8.View ArticlePubMedGoogle Scholar
- van der Rest ME, Lange C, Molenaar D: A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA. Appl Microbiol Biotechnol. 1999, 52: 541-545. 10.1007/s002530051557.View ArticlePubMedGoogle Scholar
- Niebisch A, Bott M: Molecular analysis of the cytochrome bc1-aa3 branch of the Corynebacterium glutamicum respiratory chain containing an unusual diheme cytochrome c1. Arch Microbiol. 2001, 175: 282-294. 10.1007/s002030100262.View ArticlePubMedGoogle Scholar
- Möker N, Brocker M, Schaffer S, Krämer R, Morbach S, Bott M: Deletion of the genes encoding the MtrA-MtrB two-component system of Corynebacterium glutamicum has a strong influence on cell morphology, antibiotics susceptibility and expression of genes involved in osmoprotection. Mol Microbiol. 2004, 54: 420-438. 10.1111/j.1365-2958.2004.04249.x.View ArticlePubMedGoogle Scholar
- Lange C, Rittmann D, Wendisch VF, Bott M, Sahm H: Global expression profiling and physiological characterization of Corynebacterium glutamicum grown in the presence of L-valine. Appl Environ Microbiol. 2003, 69: 2521-2532. 10.1128/AEM.69.5.2521-2532.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Smyth GK: Limma: linear models for microarray data. Bioinformatics and Computational Biology Solutions Using R and Bioconductor. Edited by: Gentleman R, Carey VJ, Huber W, Irizarry RA, Dudoit S. 2005, New York, NY: Springer, 397-420.View ArticleGoogle Scholar
- Edgar R, Domrachev M, Lash AE: Gene Expression Omnibus: NCBI gene expression and hybridization array data repository. Nucleic Acids Res. 2002, 30 (1): 207-210. 10.1093/nar/30.1.207.PubMed CentralView ArticlePubMedGoogle Scholar
- Garcia-Nafria J, Baumgart M, Bott M, Wilkinson AJ, Wilson KS: The Corynebacterium glutamicum aconitase repressor: scratching around for crystals. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2010, 66: 1074-1077. 10.1107/S1744309110029015.PubMed CentralView ArticlePubMedGoogle Scholar
- Niebisch A, Kabus A, Schultz C, Weil B, Bott M: Corynebacterial protein kinase G controls 2-oxoglutarate dehydrogenase activity via the phosphorylation status of the OdhI protein. J Biol Chem. 2006, 281: 12300-12307. 10.1074/jbc.M512515200.View ArticlePubMedGoogle Scholar
- Heyer A, Gätgens C, Hentschel E, Kalinowski J, Bott M, Frunzke J: The two-component system ChrSA is crucial for haem tolerance and interferes with HrrSA in haem-dependent gene regulation in Corynebacterium glutamicum. Microbiology. 2012, 158: 3020-3031. 10.1099/mic.0.062638-0.View ArticlePubMedGoogle Scholar
- Garcia-Nafria J, Baumgart M, Turkenburg JP, Wilkinson AJ, Bott M, Wilson KS: Crystal and solution studies reveal that the transcriptional regulator AcnR of Corynebacterium glutamicum is regulated by Citrate-Mg2+ binding to a non-canonical pocket. J Biol Chem. 2013, 288: 15800-15812. 10.1074/jbc.M113.462440.PubMed CentralView ArticlePubMedGoogle Scholar
- Anderberg SJ, Newton GL, Fahey RC: Mycothiol biosynthesis and metabolism - Cellular levels of potential intermediates in the biosynthesis and degradation of mycothiol in Mycobacterium smegmatis. J Biol Chem. 1998, 273: 30391-30397. 10.1074/jbc.273.46.30391.View ArticlePubMedGoogle Scholar
- Dobson G, Minnikin DE, Minnikin SM, Parlett JH, Goodfellow M, Ridell M, Magnusson M: Systematic analysis of complex mycobacterial lipids. Chemical Methods in Bacterial Systematics, Volume 1. Edited by: M. G, E. MD. 1985, London, UK: Academic Press, 237-265.Google Scholar
- Ludwiczak P, Brando T, Monsarrat B, Puzo G: Structural characterization of Mycobacterium tuberculosis lipoarabinomannans by the combination of capillary electrophoresis and matrix-assisted laser desorption/ionization time of-flight mass spectrometry. Anal Chem. 2001, 73: 2323-2330. 10.1021/ac001368h.View ArticlePubMedGoogle Scholar
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