Ancient origin of somatic and visceral neurons
© Nomaksteinsky et al.; licensee BioMed Central Ltd. 2013
Received: 1 February 2013
Accepted: 22 March 2013
Published: 30 April 2013
A key to understanding the evolution of the nervous system on a large phylogenetic scale is the identification of homologous neuronal types. Here, we focus this search on the sensory and motor neurons of bilaterians, exploiting their well-defined molecular signatures in vertebrates. Sensorimotor circuits in vertebrates are of two types: somatic (that sense the environment and respond by shaping bodily motions) and visceral (that sense the interior milieu and respond by regulating vital functions). These circuits differ by a small set of largely dedicated transcriptional determinants: Brn3 is expressed in many somatic sensory neurons, first and second order (among which mechanoreceptors are uniquely marked by the Brn3+/Islet1+/Drgx+ signature), somatic motoneurons uniquely co-express Lhx3/4 and Mnx1, while the vast majority of neurons, sensory and motor, involved in respiration, blood circulation or digestion are molecularly defined by their expression and dependence on the pan-visceral determinant Phox2b.
We explore the status of the sensorimotor transcriptional code of vertebrates in mollusks, a lophotrochozoa clade that provides a rich repertoire of physiologically identified neurons. In the gastropods Lymnaea stagnalis and Aplysia californica, we show that homologues of Brn3, Drgx, Islet1, Mnx1, Lhx3/4 and Phox2b differentially mark neurons with mechanoreceptive, locomotory and cardiorespiratory functions. Moreover, in the cephalopod Sepia officinalis, we show that Phox2 marks the stellate ganglion (in line with the respiratory — that is, visceral— ancestral role of the mantle, its target organ), while the anterior pedal ganglion, which controls the prehensile and locomotory arms, expresses Mnx.
Despite considerable divergence in overall neural architecture, a molecular underpinning for the functional allocation of neurons to interactions with the environment or to homeostasis was inherited from the urbilaterian ancestor by contemporary protostomes and deuterostomes.
KeywordsSensory neurons Motor neurons Evolution Transcription factors Mollusks Lophotrochozoa Lymnaea Aplysia Sepia Phox2 Brn3 Mnx
For several decades, molecular data have been used to define homologous regions in the nervous system of distant phyla. More recently, homology search has moved to the cell level, using conserved neuronal-type specific molecular signatures [1–5] (and reference  for review). This approach provides a novel window on the complexity of ancestral nervous systems, and sets the stage, with unprecedented detail, for an understanding of what has changed or been conserved during their large-scale evolution. In this paper, we undertake a comparison of sensorimotor circuits across the protostome/deuterostome boundary, which is, thus, informative about the nervous system of Urbilateria.
For this study, we turned to mollusks, for two reasons: first, they belong to the phylum Lophotrochozoa which, genetically less derived than Ecdysozoa, is particularly valuable for comparisons across Bilateria ; and second, mollusks have been studied by neurophysiologists for decades and consequently provide a unique catalogue of identified neurons with somatic or visceral functions  that allow rigorous tests of association with specific molecular signatures. Using as model systems two gastropods —the opisthobranch Aplysia californica and the pulmonate Lymnaea stagnalis— and the decapodiform cephalopod Sepia officinalis, we show that physiologically defined somatic motor and sensory neurons and visceral motoneurons share, respectively, the Mnx/Lhx3/4, Brn3 and Phox2 transcriptional signature of their vertebrate counterparts, and we discuss the evolutionary implications of this conservation across Bilateria.
Shared molecular signature of gastropod and vertebrate mechanosensory neurons
Shared molecular distinction between somatic and visceral motoneurons in gastropods, cephalopods and vertebrates
Hence, molluscan motoneurons that innervate the viscera are distinguished from those that innervate the locomotory muscles by the same transcriptional code (respectively Phox2 and Mnx/Lhx3/4) as their vertebrate counterparts.
We have shown that molecular signatures for neurons with somatic (that is, relational) versus visceral (that is, homeostatic) functions are conserved between vertebrates and mollusks. Visceral motoneurons, (such as cardiorespiratory neurons) express the orthologue of the vertebate pan-visceral determinant Phox2b in opisthobranch and pulmonate gastropods and a decapodiform cephalopod. No other transcription factor or neurotransmitter phenotype marks these neurons, specifically or exhaustively, in vertebrates, precluding a more complex signature. However, Phox2b expression is highly selective for visceral neurons (both motor and sensory) in vertebrates  and, thus, in combination with hodological criteria, constitutes a strong argument for homology. Neurons with modalities clearly equivalent to those of visceral sensory neurons in vertebrates (which monitor taste or blood pressure for example) are not described to our knowledge in mollusks, precluding exploration of this broad neuronal identity. Of note, in vertebrates, the viscerosensory phenotype is imposed by Phox2b on a somatosensory default identity , suggesting that the former is evolutionarily more recent than the latter. Locomotory (somatic) motor neurons express the orthologues of the homeobox genes Mnx1 and Lhx3/4 and VAChT in both gastropods and cephalopods, like their counterparts in the spinal cord of vertebrates. Finally, somatic sensory neurons (such as mechanoreceptors), characterized in gastropods by previous electrophysiological studies or expression of the peptide sensorin A, selectively express the orthologues of the homeodomain genes Brn3, Drgx, and Islet1 and VGluT, like their physiological counterparts in the dorsal root and trigeminal ganglia of vertebrates.
What evolutionary relationship can explain the conservation of molecular signatures in neurons with visceral versus somatic functions between deuterostomes and protostomes? The simplest hypothesis is that the cells are phylogenetically homologous, that is, one could trace their ancestry to an original neuron or neuronal cluster in the common ancestor, as was proposed for ciliary photoreceptors in annelids and vertebrates  or for branchial motoneurons in vertebrates and urochordates . This might also be the case for somatic motoneurons in vertebrates and lophotrochozoans: their distribution is spatially discrete in each phylum and reconcilable between phyla. In vertebrates, locomotory Mnx + neurons are born in a ventral Nkx6.2 + domain of the spinal cord, topologically and molecularly similar to the ventral domain of the nerve cord of annelids, which produces Mnx + /VAChT + neurons, presumably motor . In mollusks, they are restricted to the pedal ganglia, conceivably homologous to the ventral nerve cord of annelids.
On the other hand, phylogenetic homology between vertebrates and mollusks is unlikely, at least in most cases, for somatic sensory neurons and visceral motor neurons, due to their anatomical distribution, widespread in each species (Figures 1, 3, 4) and hard to reconcile between them. Moreover, Phox2 is expressed in synapomorphic structures of vertebrates (the neural crest-derived autonomic ganglia) and cephalopods (the stellate ganglion). In this case, the most likely evolutionary scenario is that, in the last common ancestor, a ‘seminal regulatory interaction’  arose between Brn3 and the somatic sensory phenotype and between Phox2 and the visceral phenotype. Although no target gene has been uncovered yet that would explain the physiological dichotomy these transcription factors specify, one can hypothesize, for example, that they direct axonal projections towards somatic versus visceral targets. Subsequently, this regulatory interaction would have been conserved, while Brn3 and Phox2 acquired additional expression sites, giving rise to novel groups of cells of the same broad type along each evolutionary lineage. According to this view, the relationship between the different kinds of somatosensory neurons or visceromotor neurons would be neither of phylogenetic homology (between species) nor of ‘sister cell types’ (within a species ), but instead, both between and within species, of ‘deep’ [51, 52] or ‘generative’  homology, akin to that proposed for bilaterian appendages.
Regardless of the exact nature of what has been conserved across the protostome-deuterostome boundary, either neuronal groups or regulatory links between transcription factors and neuronal traits, our data show that the viscerosomatic duality of the nervous system, as described in vertebrates, was already part of the urbilaterian body plan. Some of its components might be even more ancient, as suggested by expression of a Brn3 orthologue in the sensory ‘rhopalia’ of scyphozoan and cubozoan jellyfish . To our knowledge, Mnx has not been analyzed in the nervous system of cnidarians. Finally, no straightforward orthologue of Phox2 emerges from sequence analysis of paired-like homeobox genes in Cnidaria [see Additional file 1: Figure S1b], which complicates the search for a pre-bilaterian origin of the visceral nervous system.
Ganglia from adult A. californica (100 to 150 g) were dissected in artificial seawater (460 mM NaCl, 10 mM KCl, 55 mM MgCl2, 11 mM, CaCl2, 10 mM (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), pH 7.6), protease digested for two hours at 37°C, fixed with 4% paraformaldehyde (4% PFA) in phosphate buffered saline (PBS) overnight at 4°C, desheathed and dehydrated in ethanol. S. officinalis fertilized eggs were removed from their envelopes in artificial seawater and fixed overnight with 4% PFA when they reached stage 29 , dechorionated, fixed again with 4% PFA for a whole day and dehydrated in methanol. Samples destined to be sectioned were rehydrated in PBST (PBS, 0.1% Tween-20), incubated overnight at 4°C in (PBS, 15% sucrose), incubated for 50 minutes at 65°C in gelatine (PBS, 7.5% gelatine, 15% sucrose, pH 7.2), embedded in gelatine, frozen for one minute at −50°C in isopentane and kept at −80°C until sectioning. Ganglia from adult L. stagnalis (20 to 40 mm) specimens were dissected in a physiological solution (40 mM NaCl, 1.7 mM KCl, 1.5 mM MgCl2, 4.1 mM CaCl2, 10 mM HEPES, pH7.9), fixed with 4% PFA and processed for gelatine embedding as described above. cDNA from Rattus norvegicus Drgx, R. norvegicus Islet1 and R. norvegicus VGluT2 sequences and a mouse anti-Brn3a monoclonal antibody (MAB1585 Millipore, Billerica, MA, USA) were used for mice procedures.
In situhybridization on sections
Frozen sections (12 μm) were thawed and air dried, washed briefly in PBST, treated 2 × 10 minutes with RIPA buffer (150 mM NaCl, 1% NP-40, 0.5% Na deoxycholate, 0.1% SDS, 1 mM ethylenediaminetetraacetic acid (EDTA), 50 mM Tris pH 8.0), postfixed with 4% PFA for 15 minutes, washed 3 × 5 minutes in PBST, acetylated with (100 mM triethanolamine, pH 8.0, 0.25% acetic anhydride) for 15 minutes on a rocker table and washed 3 × 5 minutes in PBST. Endogenous alkaline phosphatases were inactivated by a 60-minute incubation in PBS at 80°C. The slides were then prehybridized for 60 minutes in hybridization solution (50% formamide, 5X SSC, 5X Denhardt’s, 500 μg/mL herring sperm DNA, 250 μg/mL yeast RNA) at 65°C and hybridized with the digoxigenin-labeled RNA (Roche, Penzberg, Germany) probe (500 ng/mL) overnight at the same temperature. The slides were washed twice in 50% formamide, 2X SSC, 0.1% Tween-20) for 60 minutes and in 0.2X SSC at 65°C for 60 minutes. The slides were then rinsed 3 × 10 minutes in buffer 1 (100 mM maleic acid, pH 7.5, 150 mM NaCl, 0.1% Tween-20), blocked in buffer 2 (buffer 1, 10% sheep serum) for 60 minutes and incubated overnight at 4°C with alkaline phosphatase-coupled anti-DIG antibody (Roche) diluted 1/2000 in buffer 2. The slides were washed 2 × 10 minutes in buffer 1, incubated for 30 minutes in buffer 3 (100 mM Tris, pH 9.5, 100 mM NaCl, 50 mM MgCl2, 0.1% Tween-20) and the signal was revealed in filtered buffer 4 (3.5 μL NBT (4-nitroblue tetrazolium chloride) (Roche) 100 mg/mL), 3.5 μL BCIP ((5-bromo-4-chloro-3-indoylphosphate) (Roche) 50 mg/mL) in buffer 3). The slides were washed 3 × 5 minutes in PBST and then postfixed overnight with 4% PFA, washed briefly in PBST and then either washed in water and mounted in Aquatex (Merck) or, when nerves had been retrogradely filled, treated as follows: sections were permeabilized 2 × 10 minutes in PBS, 0.3% Triton X-100, blocked 20 minutes in blocking solution (PBS, 10% fetal calf serum, 0.1% Triton X-100), incubated for two hours in the dark with ExtrAvidin-fluorescein isothiocyanate (FITC, Sigma, Saint-Quentin Fallavier, France) diluted 1/400 in blocking solution, washed 3 × 10 minutes in PBST in the dark and mounted in Mowiol (Calbiochem, Darmstadt, Germany).
In situhybridization on whole-mounts
The dehydrated desheathed CNS were progressively rehydrated in PBST, incubated for 20 minutes in PBS, 0.3% Triton X-100, rinsed in PBST, permeabilized with PBST, 10 mg/mL Proteinase K for 60 minutes, washed 2 × 5 minutes in PBST, postfixed in 4% PFA for 30 minutes, washed 2 × 5 minutes in PBST, incubated for 10 minutes in 100 mM triethanolamine, pH 8.0, acetylated 2 × 10 minutes with 100 mM triethanolamine, pH 8.0, 0.25% acetic anhydride, washed 2 × 5 minutes in PBST, incubated at 80°C in PBS for 60 minutes, rinsed in PBST, prehybridized for 60 minutes in hybridization buffer (50% formamide, 1.3X SSC, 5 mM EDTA, 50 μg/mL yeast RNA, 2% Tween-20, 0.5% CHAPS) at 65°C and hybridized overnight with the probe (200 ng/mL) at the same temperature. The samples were rinsed twice and washed 2 × 30 minutes in prewarmed hybridization solution at 65°C. Then they were washed for 10 minutes at room temperature in a 1:1 mixture of hybridization solution and buffer 1, 60 minutes in buffer 1, blocked 60 minutes in buffer 1/ 20% FCS, incubated overnight at 4°C with alkaline phosphatase-coupled anti-DIG antibody (Roche) diluted 1/2000 in buffer 1/ 2% FCS, rinsed three times and washed 3 × 60 minutes in buffer 1, then left for two days in buffer 1, incubated 2 × 30 minutes in buffer 3, revealed in the dark with filtered NBT/BCIP (Sigma), washed in the dark 3 x 10 minutes with PBST, postfixed overnight with 4% PFA, rinsed in PBST and kept at 4°C in Tissue-Tek®optimal cutting temperature (O.C.T.) embedding medium (Sakura, Tokyo, Japan). Except for revelation, all steps were performed with agitation.
Immunofluorescence for detection of mouse Brn3a
Sections were dried, washed for 5 minutes in PBS, permeabilized 2 × 10 minutes in PBS/ 0.3% Triton X-100, blocked for 20 minutes in blocking solution (PBS, 10% FCS, 0.1% Triton X-100), incubated overnight at 4°C with the mouse anti-Brn3a monoclonal antibody (MAB1585) diluted 1/200 in blocking solution, washed 3 × 10 minutes in PBST, incubated for 2 hours with the fluorescent goat anti-mouse Cy3-coupled secondary antibody (115-165-003 Jackson ImmunoResearch, Newmarket, UK), washed 3 × 10 minutes in PBST in the dark and mounted in Mowiol (Calbiochem). The image was converted to gray scale, inverted and superimposed on a Nomarski photomicrograph in Photoshop CS3.
Cloning of orthologues
Starting with total RNA extracted using RNeasy Lipid Tissue Mini Kit (Qiagen), fragments of newly cloned cDNAs were amplified by PCR and rapid amplification of cDNA ends-PCR (RACE-PCR) as described previously . Orthology was assigned by phylogenetic analysis (see below and Additional file 1: Figure S1). Ls-VAChT and Ls-VGluT were PCR-amplified using oligonucleotides derived from the GenBank sequences (accession numbers: AF484093 and AB469850, respectively).
The ganglia of a 20 to 40 mm Lymnaea stagnalis specimen were dissected in a 1:1 mixture of physiological medium with Leibovitz's L15 medium (21083–027, Life technologies, Saint Aubin, France) complemented with 50 U/mL pennicilin, 50 μg/mL streptomycin and at the final concentration of 0.3 M glucose, cutting short all nerves except those of interest. The preparation was pinned at the bottom of a sylgard dish where two compartments were delineated with Vaseline, separating the ganglia from the distal part of the nerves to be backfilled. The nerve compartment was emptied, filled with distilled water (dH2O), nerves were recut and, after a 10-minute incubation, dH2O was replaced by a saturated solution of biocytin (Sigma) in dH2O. After 24 hours at room temperature and in the dark, the ganglia were fixed overnight at 4°C in 4% PFA and processed for in situ hybridization and biocytin revelation using Extravidin-FITC (see above).
Gene orthologies were assessed using protein sequences aligned with ClustalX2  and the software PhyML  with 1,000 bootstrap replicates and the model suggested by ProtTest 2.4 . The closest groups of homeogenes were used as outgroups . Trees were drawn with Dendroscope v3.0.14 .
Experiments on mouse embryos have been approved by The Research Ethic committee « Charles Darwin », Paris (approval number Ce5/2012/064).
Central nervous system
Fetal calf serum
Light yellow cells
Polymerase chain reaction
Rapid amplification of cDNA ends-PCR.
We thank E. Konstantinov for technical assistance, H. Roest Crollius and R. de Rosa for advice on the phylogenetic analyses and C. Goridis for critical reading of the manuscript. This work was supported by a grant of the Agence Nationale pour la Recherche (to JFB and GF), of the Fondation pour la Recherche Médicale (to MN) and by institutional support from the Centre de la Recherche Scientifique (CNRS), Institut National de la Santé et de la Recherche Médicale (INSERM) and École normale supérieure.
- Arendt D, Tessmar-Raible K, Snyman H, Dorresteijn AW, Wittbrodt J: Ciliary photoreceptors with a vertebrate-type opsin in an invertebrate brain. Science. 2004, 306: 869-871. 10.1126/science.1099955.View ArticlePubMed
- Dufour HD, Chettouh Z, Deyts C, de Rosa R, Goridis C, Joly JS, Brunet JF: Precraniate origin of cranial motoneurons. Proc Natl Acad Sci USA. 2006, 103: 8727-8732. 10.1073/pnas.0600805103.PubMed CentralView ArticlePubMed
- Tessmar-Raible K, Raible F, Christodoulou F, Guy K, Rembold M, Hausen H, Arendt D: Conserved sensory-neurosecretory cell types in annelid and fish forebrain: insights into hypothalamus evolution. Cell. 2007, 129: 1389-1400. 10.1016/j.cell.2007.04.041.View ArticlePubMed
- Vopalensky P, Pergner J, Liegertova M, Benito-Gutierrez E, Arendt D, Kozmik Z: Molecular analysis of the amphioxus frontal eye unravels the evolutionary origin of the retina and pigment cells of the vertebrate eye. Proc Natl Acad Sci USA. 2012, 109: 15383-15388. 10.1073/pnas.1207580109.PubMed CentralView ArticlePubMed
- Abitua PB, Wagner E, Navarrete IA, Levine M: Identification of a rudimentary neural crest in a non-vertebrate chordate. Nature. 2012, 492: 104-107. 10.1038/nature11589.PubMed CentralView ArticlePubMed
- Arendt D: The evolution of cell types in animals: emerging principles from molecular studies. Nat Rev Genet. 2008, 9: 868-882. 10.1038/nrg2416.View ArticlePubMed
- Bichat X: Recherches physiologiques sur la vie et la mort. 1802, Paris: Brosson et GabonView Article
- Guthrie S: Patterning and axon guidance of cranial motor neurons. Nat Rev Neurosci. 2007, 8: 859-871. 10.1038/nrn2254.View ArticlePubMed
- D'Amico-Martel A, Noden DM: Contributions of placodal and neural crest cells to avian cranial peripheral ganglia. Am J Anat. 1983, 166: 445-468. 10.1002/aja.1001660406.View ArticlePubMed
- Romer AS: The vertebrate as a dual animal -somatic and visceral. Evol Biol. 1972, 6: 121-156.View Article
- Badea TC, Williams J, Smallwood P, Shi M, Motajo O, Nathans J: Combinatorial expression of brn3 transcription factors in somatosensory neurons: genetic and morphologic analysis. J Neurosci. 2012, 32: 995-1007. 10.1523/JNEUROSCI.4755-11.2012.PubMed CentralView ArticlePubMed
- D'Autréaux F, Coppola E, Hirsch MR, Birchmeier C, Brunet JF: Homeoprotein Phox2b commands a somatic-to-visceral switch in cranial sensory pathways. Proc Natl Acad Sci USA. 2011, 108: 20018-20023. 10.1073/pnas.1110416108.PubMed CentralView ArticlePubMed
- Arber S, Han B, Mendelsohn M, Smith M, Jessell TM, Sockanathan S: Requirement for the homeobox gene Hb9 in the consolidation of motor neuron identity. Neuron. 1999, 23: 659-674. 10.1016/S0896-6273(01)80026-X.View ArticlePubMed
- Sharma K, Sheng HZ, Lettieri K, Li H, Karavanov A, Potter S, Westphal H, Pfaff SL: LIM homeodomain factors Lhx3 and Lhx4 assign subtype identities for motor neurons. Cell. 1998, 95: 817-828. 10.1016/S0092-8674(00)81704-3.View ArticlePubMed
- Pattyn A, Morin X, Cremer H, Goridis C, Brunet JF: The homeobox gene Phox2b is essential for the development of autonomic neural crest derivatives. Nature. 1999, 399: 366-370. 10.1038/20700.View ArticlePubMed
- Brunet JF, Pattyn A: Phox2 genes - from patterning to connectivity. Curr Opin Genet Dev. 2002, 12: 435-440. 10.1016/S0959-437X(02)00322-2.View ArticlePubMed
- Goridis C, Dubreuil V, Thoby-Brisson M, Fortin G, Brunet JF: Phox2b, congenital central hypoventilation syndrome and the control of respiration. Semin Cell Dev Biol. 2010, 21: 814-822. 10.1016/j.semcdb.2010.07.006.View ArticlePubMed
- Raible F, Arendt D: Metazoan evolution: some animals are more equal than others. Curr Biol. 2004, 14: 106-108.View Article
- Kandel ER: Behavioural Biology of Aplysia: Contribution to the Comparative Study of Opisthobranch Molluscs. 1979, San Francisco: WH Freeman
- Clyne PJ, Certel SJ, de Bruyne M, Zaslavsky L, Johnson WA, Carlson JR: The odor specificities of a subset of olfactory receptor neurons are governed by Acj6, a POU-domain transcription factor. Neuron. 1999, 22: 339-347. 10.1016/S0896-6273(00)81094-6.View ArticlePubMed
- Finney M, Ruvkun G: The unc-86 gene product couples cell lineage and cell identity in C. elegans. Cell. 1990, 63: 895-905. 10.1016/0092-8674(90)90493-X.View ArticlePubMed
- O'Brien EK, Degnan BM: Developmental expression of a class IV POU gene in the gastropod Haliotis asinina supports a conserved role in sensory cell development in bilaterians. Dev Genes Evol. 2002, 212: 394-398. 10.1007/s00427-002-0256-x.View ArticlePubMed
- Brunet JF, Shapiro E, Foster SA, Kandel ER, Iino Y: Identification of a peptide specific for Aplysia sensory neurons by PCR-based differential screening. Science. 1991, 252: 856-859. 10.1126/science.1840700.View ArticlePubMed
- Rebelo S, Reguenga C, Osório L, Pereira C, Lopes C, Lima D: DRG11 immunohistochemical expression during embryonic development in the mouse. Dev Dyn. 2007, 236: 2653-2660. 10.1002/dvdy.21271.View ArticlePubMed
- Dykes IM, Tempest L, Lee SI, Turner EE: Brn3a and Islet1 act epistatically to regulate the gene expression program of sensory differentiation. J Neurosci. 2011, 31: 9789-9799. 10.1523/JNEUROSCI.0901-11.2011.PubMed CentralView ArticlePubMed
- Dale N, Kandel ER: L-glutamate may be the fast excitatory transmitter of Aplysia sensory neurons. Proc Natl Acad Sci USA. 1993, 90: 7163-7167. 10.1073/pnas.90.15.7163.PubMed CentralView ArticlePubMed
- Lacaze-Duthiers H: Du système nerveux des mollusques gastéropodes pulmonés aquatiques et d’un nouvel organe d’innervation. Arch zool exptl et gén. 1872, 1: 437-700.
- Broihier HT, Skeath JB: Drosophila homeodomain protein dHb9 directs neuronal fate via crossrepressive and cell-nonautonomous mechanisms. Neuron. 2002, 35: 39-50. 10.1016/S0896-6273(02)00743-2.View ArticlePubMed
- Odden JP, Holbrook S, Doe CQ: Drosophila HB9 is expressed in a subset of motoneurons and interneurons, where it regulates gene expression and axon pathfinding. J Neurosci. 2002, 22: 9143-9149.PubMed
- Von Stetina SE, Fox RM, Watkins KL, Starich TA, Shaw JE, Miller DM: UNC-4 represses CEH-12/HB9 to specify synaptic inputs to VA motor neurons in C. elegans. Genes Dev. 2007, 21: 332-346. 10.1101/gad.1502107.PubMed CentralView ArticlePubMed
- Denes AS, Jékely G, Steinmetz PR, Raible F, Snyman H, Prud'homme B, Ferrier DE, Balavoine G, Arendt D: Molecular architecture of annelid nerve cord supports common origin of nervous system centralization in bilateria. Cell. 2007, 129: 277-288. 10.1016/j.cell.2007.02.040.View ArticlePubMed
- Pujol N, Torregrossa P, Ewbank JJ, Brunet JF: The homeodomain protein CePHOX2/CEH-17 controls antero-posterior axonal growth in C. elegans. Development. 2000, 127: 3361-3371.PubMed
- Van Buskirk C, Sternberg PW: Paired and LIM class homeodomain proteins coordinate differentiation of the C. elegans ALA neuron. Development. 2010, 137: 2065-2074. 10.1242/dev.040881.PubMed CentralView ArticlePubMed
- Alevizos A, Bailey CH, Chen M, Koester J: Innervation of vascular and cardiac muscle of Aplysia by multimodal motoneuron L7. J Neurophysiol. 1989, 61: 1053-1063.PubMed
- Kurokawa M, Ohsuga K, Kuwasawa K: A reexamination of the synaptic connection between neuron L7 of the abdominal ganglion and neurons of the branchial ganglion in Aplysia californica, A. kurodai and A. juliana. Neurosci Lett. 1998, 241: 49-52. 10.1016/S0304-3940(97)00954-3.View ArticlePubMed
- Coggeshall RE, Kandel ER, Kupfermann I, Waziri R: A morphological and functional study on a cluster of identifiable neurosecretory cells in the abdominal ganglion of aplysia californica. J Cell Biol. 1966, 31: 363-368. 10.1083/jcb.31.2.363.PubMed CentralView ArticlePubMed
- Rittenhouse AR, Price CH: Electrophysiological and anatomical identification of the peripheral axons and target tissues of Aplysia neurons R3-14 and their status as multifunctional, multimessenger neurons. J Neurosci. 1986, 6: 2071-2084.PubMed
- Frazier WT, Kandel ER, Kupfermann I, Waziri R, Coggeshall RE: Morphological and functional properties of identified neurons in the abdominal ganglion of Aplysia Californica. J Neurophysiol. 1967, 30: 1288-1351.
- Morishita F, Nakanishi Y, Sasaki K, Kanemaru K, Furukawa Y, Matsushima O: Distribution of the Aplysia cardioexcitatory peptide, NdWFamide, in the central and peripheral nervous systems of Aplysia. Cell Tissue Res. 2003, 312: 95-111.PubMed
- Campanelli JT, Scheller RH: Histidine-rich basic peptide: a cardioactive neuropeptide from Aplysia neurons R3-14. J Neurophysiol. 1987, 57: 1201-1209.PubMed
- Landry C, Crine P, DesGroseillers L: Differential expression of neuropeptide gene mRNA within the LUQ cells of Aplysia californica. J Neurobiol. 1992, 23: 89-101. 10.1002/neu.480230109.View ArticlePubMed
- Wendelaar Bonga SE: Ultrastructure and histochemistry of neurosecretory cells and neurohaemal areas in the pond snail Lymnaea stagnalis (L.). Z Zellforsch Mikrosk Anat. 1970, 108: 190-224. 10.1007/BF00335295.View ArticlePubMed
- Smit AB, Hoek RM, Geraerts WP: The isolation of a cDNA encoding a neuropeptide prohormone from the light yellow cells of Lymnaea stagnalis. Cell Mol Neurobiol. 1993, 13: 263-270. 10.1007/BF00733754.View ArticlePubMed
- Boer HH, Montagne-Wajer C: Functional morphology of the neuropeptidergic light-yellow-cell system in pulmonate snails. Cell Tissue Res. 1994, 277: 531-538. 10.1007/BF00300226.View ArticlePubMed
- Elo JE: Das nervensystem von limnaea stagnalis (L.) Lam. Ann Zool Soc Zool-Bot Fenn Vanamo. 1938, 6: 1-40.
- Boycott BB: The functional organization of the brain of the cuttlefish sepia officinalis. Proc Roy Soc B. 1961, 153: 503-504. 10.1098/rspb.1961.0015.View Article
- Young JZ: The nervous system of Loligo. II. Suboesophageal centres. Proc Roy Soc B. 1976, 274: 101-167.
- Coppola E, D'Autréaux F, Nomaksteinsky M, Brunet JF: Phox2b expression in the taste centers of fish. J Comp Neurol. 2012, 520: 3633-3649. 10.1002/cne.23117.View ArticlePubMed
- Young JZ: Fused neurons and synaptic contacts in the giant nerve fibres of cephalopods. Phil Trans R Soc Lond B. 1939, 229: 465-503. 10.1098/rstb.1939.0003.View Article
- Scott MP: Intimations of a creature. Cell. 1994, 79: 1121-1124. 10.1016/0092-8674(94)90001-9.View ArticlePubMed
- Shubin N, Tabin C, Carroll S: Fossils, genes and the evolution of animal limbs. Nature. 1997, 388: 639-648. 10.1038/41710.View ArticlePubMed
- Shubin N, Tabin C, Carroll S: Deep homology and the origins of evolutionary novelty. Nature. 2009, 457: 818-823. 10.1038/nature07891.View ArticlePubMed
- Butler AB, Saidel WM: Defining sameness: historical, biological, and generative homology. Bioessays. 2000, 22: 846-853. 10.1002/1521-1878(200009)22:9<846::AID-BIES10>3.0.CO;2-R.View ArticlePubMed
- Nakanishi N, Yuan D, Hartenstein V, Jacobs DK: Evolutionary origin of rhopalia: insights from cellular-level analyses of Otx and POU expression patterns in the developing rhopalial nervous system. Evol Dev. 2010, 12: 404-415. 10.1111/j.1525-142X.2010.00427.x.View ArticlePubMed
- Lemaire J: Table de développement embryonnaire de Sepia Officinalis L. (Mollusque Céphalopode). Bull Soc Zool France. 1970, 95: 773-782.
- Nomaksteinsky M, Röttinger E, Dufour HD, Chettouh Z, Lowe CJ, Martindale MQ, Brunet JF: Centralization of the deuterostome nervous system predates chordates. Curr Biol. 2009, 19: 1264-1269. 10.1016/j.cub.2009.05.063.View ArticlePubMed
- Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG: Clustal W and Clustal X version 2.0. Bioinformatics. 2007, 23: 2947-2948. 10.1093/bioinformatics/btm404.View ArticlePubMed
- Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol. 2003, 52: 696-704. 10.1080/10635150390235520.View ArticlePubMed
- Abascal F, Zardoya R, Posada D: ProtTest: selection of best-fit models of protein evolution. Bioinformatics. 2005, 21: 2104-2105. 10.1093/bioinformatics/bti263.View ArticlePubMed
- Larroux C, Luke GN, Koopman P, Rokhsar DS, Shimeld SM, Degnan BM: Genesis and expansion of metazoan transcription factor gene classes. Mol Biol Evol. 2008, 25: 980-996. 10.1093/molbev/msn047.View ArticlePubMed
- Huson DH, Richter DC, Rausch C, Dezulian T, Franz M, Rupp R: Dendroscope: an interactive viewer for large phylogenetic trees. BMC Biol. 2007, 8: 460-
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.