Pioglitazone leads to an inactivation and disassembly of complex I of the mitochondrial respiratory chain
© García-Ruiz et al.; licensee BioMed Central Ltd. 2013
Received: 10 April 2013
Accepted: 16 July 2013
Published: 1 August 2013
Thiazolidinediones are antidiabetic agents that increase insulin sensitivity but reduce glucose oxidation, state 3 respiration, and activity of complex I of the mitochondrial respiratory chain (MRC). The mechanisms of the latter effects are unclear. The aim of this study was to determine the mechanisms by which pioglitazone (PGZ), a member of the thiazolidinedione class of antidiabetic agents, decreases the activity of the MRC. In isolated mitochondria from mouse liver, we measured the effects of PGZ treatment on MRC complex activities, fully-assembled complex I and its subunits, gene expression of complex I and III subunits, and [3H]PGZ binding to mitochondrial complexes.
In vitro, PGZ decreased activity of complexes I and III of the MRC, but in vivo only complex I activity was decreased in mice treated for 12 weeks with 10 mg/kg/day of PGZ. In vitro treatment of isolated liver mitochondria with PGZ disassembled complex I, resulting in the formation of several subcomplexes. In mice treated with PGZ, fully assembled complex I was increased and two additional subcomplexes were found. Formation of supercomplexes CI+CIII2+CIVn and CI+CIII2 decreased in mouse liver mitochondria exposed to PGZ, while formation of these supercomplexes was increased in mice treated with PGZ. Two-dimensional analysis of complex I using blue native/sodium dodecyl sulfate polyacrylamide gel electrophoresis (BN/SDS-PAGE) showed that in vitro PGZ induced the formation of four subcomplexes of 600 (B), 400 (C), 350 (D), and 250 (E) kDa, respectively. Subcomplexes B and C had NADH:dehydrogenase activity, while subcomplexes C and D contained subunits of complex I membrane arm. Autoradiography and coimmunoprecipitation assays showed [3H]PGZ binding to subunits NDUFA9, NDUFB6, and NDUFA6. Treatment with PGZ increased mitochondrial gene transcription in mice liver and HepG2 cells. In these cells, PGZ decreased intracellular ATP content and enhanced gene expression of specific protein 1 and peroxisome-proliferator activated receptor (PPAR)γ coactivator 1α (PGC-1α).
PGZ binds complex I subunits, which induces disassembly of this complex, reduces its activity, depletes cellular ATP, and, in mice and HepG2 cells, upregulates nuclear DNA-encoded gene expression of complex I and III subunits.
KeywordsATP Mitochondrial respiratory chain Pioglitazone Proteomic Thiazolidinediones
Pioglitazone (PGZ), a member of the thiazolidinedione (TZD) class of antidiabetic agents and agonist of the peroxisome proliferator-activated receptor γ (PPARγ) , improves insulin sensitivity both in the liver and peripheral tissues. The mechanisms of this effect are unclear. However, they are attributed to the actions of TZDs on PPARγ . After activating PPARγ receptors, TZDs induce adipocyte differentiation and remodeling of adipose tissue in vitro and in vivo[2, 3]. It is believed that signals derived from the adipose tissue (fatty acids, adiponectin, resistin, leptin) may mediate the improvement in skeletal glucose disposal induced by TZDs [2, 3]. However, it is also likely that other mechanisms independent of PPARγ may contribute to TZD effects on insulin sensitivity . Thus, in vitro studies have shown that TZDs elevate lactate production by skeletal muscle , suggesting an inhibition of cell respiration . In fact, several authors have found that the activity of complex I of the mitochondrial respiratory chain (MRC), state 3 respiration, and glucose oxidation were reduced in homogenates of skeletal muscle treated with increased doses of TZDs [7, 8]. PPARγ does not seem to be involved in these effects of TZDs , since they inhibited complex I in sonicated tissue homogenates containing disrupted mitochondria .
Complex I (NADH:ubiquinone oxidoreductase) is the first and the largest of the four multiprotein complexes that constitute the MRC involved in oxidative phosphorylation . This complex is formed by at least 44 subunits, 7 of which are encoded by the mitochondrial genome and the remaining 37 by the nuclear genome . The crystal structure of the entire Thermus thermophilus complex I has been recently reported . In previous studies, we have shown that PGZ suppressed the activity of complex I of the MRC in ob/ob mice, but the mechanisms of this effect are still unclear .
The aim of the present study was to determine the mechanisms by which PGZ decreases MRC activity. We show that PGZ binds subunits located in the membrane arm of complex I of the MRC, which induces disassembly of this complex, reduces its enzymatic activity, depletes cellular ATP, and consequently upregulates nuclear DNA-encoded gene expression of complex I subunits.
PGZ decreased activity of complexes I and III of the MRC in isolated mouse liver mitochondria
The activity of complex II (succinate dehydrogenase complex), which passes electrons directly to ubiquinone, was not affected significantly by PGZ treatment (control, 68.96 ± 4.8 nmol/min/mg protein; 15 μM PGZ, 67.96 ± 5.3 nmol/min/mg protein) (Figure 1A).
Ubiquinone passes electrons from complex I and II to the b-c1 complex (complex III), which transfers them to cytochrome c. The activity of complex III also significantly decreased from 85.71 ± 4.6 nmol/min/mg protein (control) to 64.20 ± 3.7 nmol/min/mg protein (P <0.001) (15 μM PGZ).
Cytochrome c is involved in carrying electrons from the b-c1 complex to the cytochrome oxidase complex (complex IV), which finally transfers these electrons to oxygen. Measurement of the activity of this complex in liver mitochondria exposed to increasing concentrations of PGZ revealed that this drug did not affect complex IV activity (controls, 47.21 ± 6.1 nmol/min/mg protein; 15 μM PGZ, 45.61 ± 5.7 nmol/min/mg protein; not significant (NS)) (Figure 1A).
ATP synthase or complex V of the oxidative phosphorylation pathway converts ADP into ATP when protons flow back from the intermembrane space into the mitochondrial matrix. The activity of this complex was not significantly affected by treatment with PGZ (controls, 223 ± 38.6 nmol/min/mg protein; 15 μM PGZ, 215.7 ± 33.1 nmol/min/mg protein; NS) (Figure 1A).
Treatment of mice with PGZ decreased activity of complex I of the MRC
We also studied the effects of PGZ on the activity of MRC complexes in C57BL/6J mice. In these animals, treatment with 10 mg PGZ/kg/day for 12 weeks significantly decreased the activity of complex I (Figure 1B) from 52.28 ± 7.16 nmol/min/mg protein in control animals to 29.32 ± 4.62 nmol/min/mg protein in PGZ-treated mice (P <0.001), but not the activity of complexes II (control, 50.81 ± 6.74 nmol/min/mg protein; PGZ, 48.23 ± 5.33 nmol/min/mg protein; NS), III (control, 72.70 ± 8.3 nmol/min/mg protein; PGZ, 73.92 ± 6.28 nmol/min/mg protein; NS), IV (control, 56.13 ± 6.81 nmol/min/mg protein; PGZ, 53.53 ± 7.11 nmol/min/mg protein; NS), and V (274.46 ± 36.27 nmol/min/mg protein; PGZ, 275.17 ± 31.63 nmol/min/mg protein; NS) (Figure 1B).
Treatment with PGZ disassembled complex I in vitro and in vivo
To determine whether PGZ treatment also alters assembly of MRC complexes in mice, liver mitochondria isolated from C57BL/6J mice untreated and treated for 12 weeks with 10 mg/kg/day PGZ via intragastric intubation were separated using a one-dimensional blue native polyacrylamide gel electrophoresis (BN-PAGE) system and analyzed by western blot using antibody against the complex I subunit NDUFA9, complex II subunit SDHA, complex III subunit core 2 (UQCRC2), complex IV subunit COX4, and complex V subunit ATP5A1. As Figure 2C illustrates, animals treated with PGZ displayed a clear increase in the fully-assembled complex I as compared to untreated, control mice. In addition, similar to in vitro studies, two other subcomplexes of 600 and 400 kDa, respectively, were detected. The bands corresponding to the remaining four complexes were not affected by PGZ treatment. Although fully assembled complex I was increased in PGZ-treated mice, its in-gel activity was clearly decreased (Figure 2D).
PGZ altered formation of respiratory supercomplexes
To investigate how PGZ affects the formation of respiratory supercomplexes, we performed BN-PAGE using the detergent digitonin instead of n-dodecylmaltoside. This condition allows loose interaction to remain intact. We found that complex I was present in the monomeric form and in two additional supercomplexes also containing complex III dimer (CI+CIII2) or complex III dimer and complex IV (CI+CIII2+CIVn). Treatment of liver mitochondria with 10 μM PGZ led to a marked decrease in the monomeric complex I and in both supercomplexes CI+CIII2 and CI+CIII2+CIVn. This decrease in supercomplex formation was due to a reduced accumulation of complexes I, III, and IV (Figure 2E). Moreover, formation of supercomplex CIII+CIV was increased after PGZ treatment.
Compared to untreated mice, in liver mitochondria isolated from mice treated with 10 mg/kg/day PGZ for 12 weeks, we found that monomeric complex I was unchanged and supercomplexes CI+CIII2+CIVn and CI+CIII2 were markedly increased. Likewise, supercomplex CIII+CIV was also augmented (Figure 2F).
PGZ disassembled the membrane arm of complex I
Two-dimensional analysis of complex I confirmed that treatment of liver mitochondria with 10 μM PGZ for 30 minutes disassembled complex I as indicated by the presence of its subunits in the mature fully assembled complex (Figure 3B, subcomplex A) and four additional subcomplexes of about 600, 400, 350, and 250 kDa, respectively (Figure 3B, subcomplexes B, C, D, and E). Subcomplex B contained subunits NDUFV1, NDUFV2, and NDUFS1 that are part of the NADH:dehydrogenase module, NDUFB6, NDUFB8, and MTND6, belonging to the membrane arm of the complex, and subunits NDUFA9, NDUFA6, NDUFS3, and NDUFS7 forming part of the hydrogenase module of complex I. Subunits NDUFV1, NDUFV2, and NDUFS1 and subunits NDUFA9, NDUFA6, NDUFS3, and NDNFS7 belonging to the hydrogenase module were present in subcomplex C. The subcomplex D of about 350 kDa contained at least subunits MTND6, NDUFB6, and NDUFB8, all of them forming part of the membrane arm. Finally, subcomplex E contained at least subunits NDUFC2 and MTND4, also members of the membrane arm.
Examination of representative subunits of complexes II (SDHA), III (UQCRC2 = core 2), IV (COX4), and V (ATP5A1) showed that only subunit core 2 was modified by treatment with PGZ (Figure 3B). Thus, this subunit appeared distributed in one additional subcomplex of about 300 kDa.
PGZ decreased prohibitin and assembly factors NDUFAF1 (CIA30), and FOXRED1 in mouse liver mitochondria
[3H]PGZ binds to complex I subunits NDUFA9, NDUFB6, and NDUFA6
To investigate whether PGZ interacts with complex I or with any of its subunits, we exposed isolated liver mitochondria to 10 μM [3H]PGZ for 30 minutes. As shown in Figure 4C, [3H]PGZ binds to mitochondrial proteins of about 980, 600, and 400 kDa molecular weight. To identify subunits that PGZ was bound to, we treated isolated mitochondria with 10 μM [3H]PGZ and complex I subunits were separated in parallel on two SDS/BN-PAGE gels as indicated in Methods. Autoradiography showed that [3H]PGZ was bound particularly to a subunit of 39 kDa, but also to subunits of 17 and 15 kDa that overlapped the signals originated by NDUFA9, NDUFB6, and NDUFA6, respectively (Figure 4D). The binding of [3H]PGZ to these subunits was confirmed by immunoprecipitating mitochondrial subunits after treating isolated mitochondria with 10 μM [3H]PGZ for 30 minutes (Figure 4E,F).
Treatment with PGZ increased mitochondrial gene transcription in mice liver and HepG2 cells
To ensure that PGZ increases gene expression of MRC subunits, we treated HepG2 cells with 10 μM PGZ for 5 days and measured expression of complex I subunits NDUFA9, NDUFB6, NDUFS3, complex III subunits UQCRC1, UQCRC2, and UQCRFS1, complex IV subunit COX4, and complex II subunit SDHA. As Figure 5B shows, treatment of cells with PGZ increased gene expression of complex I subunits by 30% and complex III by 20% to 30%, but did not increase significantly gene expression of complex II and IV subunits.
PGZ reduced intracellular ATP content and upregulated gene expression of specific protein 1 (Sp1) and PPARγ coactivator 1α (PGC-1α) in HepG2 cells
To determine whether PGZ modifies intracellular ATP content, HepG2 cells were cultured in the absence or presence of 10 μM PGZ for 5 days. As Figure 5C shows, treatment of cells with 10 μM PGZ decreased cellular ATP from 10.93 ± 0.85 nmol/mg protein to 4.18 ± 0.42 nmol/mg protein (P <0.0001). To examine whether PGZ modifies gene expression of transcription factors involved in the regulation of gene encoding MRC proteins, we exposed HepG2 cells to 10 μM PGZ for 5 days and measured gene expression of Sp1, estrogen related receptor α (ERRα), and PGC-1α of the MRC by real-time polymerase chain reaction (RT-PCR). We found that PGZ treatment increased significantly gene expression of Sp1 and PGC-1α, but not of ERRα (Figure 5D).
Our results shows that in vitro PGZ significantly inhibits complex I activity and, less intensely but also significantly, complex III in isolated liver mitochondria. Likewise, treatment of mice with PGZ for 12 weeks at a dose that has demonstrated to have pharmacologic effects (10 mg PGZ/g/day)  resulted in a significant decrease in complex I, but not complex III, activity. These results are consistent with those obtained by others with PGZ and other TZDs [7, 8, 15] and with those we have previously reported in ob/ob mice . Although some authors found that this effect of PGZ on complex I activity was not dose-dependent , we found a clear relationship between the dose of PGZ and the decrease in complex activity (Figure 1A). In contrast, we found no effects of PGZ on complex II, IV, or V activity.
Mechanisms by which PGZ decreases complex I and III activity are not well understood. Complex I transfers two electrons from NADH to ubiquinone, represents the main entrance site for electrons into the MRC, and translocates four protons across the mitochondrial inner membrane . It is composed of two arms forming an L-shaped structure. One arm is hydrophobic and is embedded in the inner mitochondrial membrane, whereas the other arm is hydrophilic and protrudes into the mitochondrial matrix. While the hydrophilic arm is formed by subunits involved in electron transfer from NADH to ubiquinone, the membrane arm is responsible for the proton translocation .
Activity of complex I can be reduced as a result of a large number of factors including mutations in complex I subunits , physiological molecules, such as nitric oxide , drugs, such as rotenone and TZD [7, 15, 20], or oxygen or nitrogen derived reactive substances such as peroxynitrite . Moreover, defects in the assembly/stability of this complex may also result in a reduced activity of complex I.
Mechanisms by which PGZ destabilizes complex I are unclear. A number of factors have been recognized to be implicated in the assembly/stabilization of complex I, including complex III , NDUFAF1 (CIA30) , NDUFAF2 (B17.2L) , NDUFA9 complex I subunit , FOXRED1 , and prohibitin , among others. Although our study shows that in vitro treatment of isolated mitochondria with PGZ markedly altered these proteins, we do not believe that the PGZ-induced disassembly of complex I resulted from the effects of PGZ on these stabilizing proteins, as some of these proteins (NDUFAF1, NDUFAF2) dissociate before complex I is completely assembled and are not found in the mature holoenzyme [23, 24]. Prohibitin, a protein that likely prevents complex degradation by AAA-metalloproteinases , decreased in parallel with fully assembled complex I (Figure 4A). However, our study cannot differentiate whether PGZ-induced complex I fragmentation was the result of the reduced amount of prohibitin or, on the contrary, prohibitin bound to complex I was reduced because PGZ had decreased fully assembled complex I. In the case of NDUFAF1, a chaperone not found associated with mature complex I, PGZ induced its binding to a new complex of 600 kDa, likely the subcomplex B formed after fragmentation of complex I. (Figures 4A,B, and 6). The effect of PGZ on FOXRED1 is difficult to interpret as very little is known about the function of this protein . We are not aware of other studies that have been published on the effects of TZDs on prohibitin or other chaperone proteins.
Our study also shows the binding of radiolabeled PGZ to mature complex I and subcomplexes of 600 and 400 kDa of molecular weight (Figure 4C). Little information is available in the literature concerning the interaction of TZD with complex I proteins, although it has been suggested that PPARγ ligands interact directly with this complex . Only Colca et al. have studied the interaction of PGZ with mitochondrial membranes and found that radiolabeled PGZ binds to a 17-kDa mitochondrial protein . Our study shows the binding of PGZ to complex I components, particularly to NDUFA9, NDUFB6, and NDUFA6 subunits (Figure 4). These subunits are hydrophobic and are situated in the inner mitochondrial membrane. TZD, like other inhibitors of this complex , are highly lipophilic and bind mitochondrial membrane . Thus, we could speculate that molecular changes induced by the binding of PGZ to these subunits may destabilize complex I resulting in the progressive fragmentation of its membrane arm.
The decrease in complex III activity observed after in vitro treatment of mitochondria with PGZ may be ascribed to the fact that complexes I and III interact physically to form supercomplexes that improves electron transfer between them  and stabilizes complex III . Therefore, PGZ-induced disassembly of complex I may also indirectly compromise complex III stability and activity. In accordance with these facts, our study shows that in vitro addition of PGZ to isolated mitochondria reduced not only the amount of complex I, but also the formation of supercomplexes CI+CIII2+CIVn and CI+CIII2 (Figure 2E). In contrast, supercomplexes CI+CIII2+CIVn and CI+CIII2 were clearly increased in mice treated with PGZ for 12 weeks. This increase in the formation of supercomplexes in PGZ-treated mice, may justify why complex III activity remained normal in these animals (Figure 2F). This elevated accumulation of complex I and III to form supercomplexes might be a consequence of the association of MRC complexes to form more efficient respiratory structures  that might compensate the reduced activity of complex I and III of the MRC. This increase in supercomplex formation may also contribute to the elevated synthesis of complex I and III subunits.
The present study also shows that treatment of mice with PGZ for 12 weeks increased the amount of fully assembled complex I (Figure 2C), although its activity was significantly decreased (Figures 1B and 2D). Similar to what occurred in in vitro studies, these in vivo studies demonstrated the presence of two additional subcomplexes of 600 kDa and 400 kDa (Figure 2A) indicating that complex I was also disassembled. The increased amount of fully assembled complex I found in PGZ-treated mice may be ascribed to the fact that gene expression of nuclear DNA-encoded complex I subunits was significantly increased (Figure 5A). Our study shows that PGZ increased gene expression of complex I and, to a lesser extent, complex III subunits in mice and HepG2 cells (Figure 5A,B). These effects may be interpreted as a compensatory response to the decreased activity of these complexes found after treatment with PGZ. Transcription of MRC subunits is coordinated with energetic needs of the cells. Thus, the cellular pool of ATP, the primary end product of oxidative phosphorylation, plays a key role in the control of nuclear and mitochondrial transcription [33, 34]. In the present study we clearly show that incubation of HepG2 cells with PGZ significantly decreased cellular content of ATP (Figure 4C). Therefore, it might be expected that decreased ATP formation as a result of the PGZ-induced complex I inhibition might lead to an increased transcription of some components of the MRC. The lack of increased expression of mtDNA-encoded subunits in PGZ-treated mice may be attributed to the reduced ability of cells to repair mtDNA damage . A large number of transcription factors have been shown to be involved in the regulation of genes encoding MRC proteins and assembly factors, including stimulatory protein 1 (Sp1), ERRα, and transcriptional coactivator PPARγ (PGC1α), among others . In particular, transcriptional coactivator PGC1α plays a critical role in adaptation mechanisms to caloric restriction and ATP reduction . In our study, we show that treatment of mice with PGZ or exposition of HepG2 cells to PGZ induced the expression of transcription factors Sp1 and particularly PGC1α (Figure 5D,E). Very little information is available about the effects of TZD on gene expression of components of the MRC and its regulatory factors. Strum et al. found that rosiglitazone increases expression of genes regulating mitochondrial biogenesis in mice and that this effect was associated with an increased level of ERRα mRNA but not of PGC1α mRNA . However, Boden et al. found that troglitazone and other TZDs, upregulated gene expression and synthesis of proteins involved in electron transport and oxidative phosphorylation in subcutaneous fat , and Mensink et al. demonstrated that rosiglitazone increased PGC1α gene expression in patients with type 2 diabetes mellitus .
In summary, in the present study we show that PGZ binds complex I subunits, likely NDUFA9, NDUB6, and NDUFA6, which may induce disassembly of this complex, reduces its activity, depletes cellular ATP, and, in mice and HepG2 cells, upregulates nuclear DNA-encoded gene expression of complex I and III subunits. Elucidation of the molecular mechanisms by which PGZ binding provokes disassembly of complex I requires further studies.
The effects of PGZ on the MRC were explored in mitochondrial complexes isolated from the liver of C57BL/6J mouse and from the hepatocellular carcinoma cell line (HepG2; American Type Culture Collection (ATCC)) cultured in Dulbecco’s modified Eagle medium (Lonza Iberica SA, Barcelona, Spain) with 10% fetal calf serum, 1% l-glutamine, 1% penicillin, 1% streptomycin, 1% fungizone.
All procedures on mice were carried out in accordance with the Spanish Guidelines for the Care and Use of Laboratory Animals. The 6-week-old male C57BL/6J mice were purchased from Charles River Laboratory (Charles River Laboratories España SA, Santa Perpetua de la Mogoda, Spain). Animals were housed at constant room temperature (23°C) (n = 3 per cage) under 12 h light/dark cycles with ad libitum access to water and standard laboratory mouse chow. A total of 20 mice were distributed into two groups: group I (control) included 10 untreated mice; group II (PGZ) consisted of 10 mice treated with 10 mg/kg/day of PGZ by daily gavage (Hartmann Analytic, Grupo Taper SA, Alcobendas, Spain) for 12 weeks. Food but not water was withdrawn overnight before mice were killed. The last PGZ dose was administered 20 h before tissue harvesting. Following the treatment, animals were anesthetized and killed at 18 weeks of age, and the liver was rapidly harvested for further analysis.
Extraction of MRC complexes from mouse liver
Mitochondrial isolation from mouse liver samples was carried as described by Schägger et al.  with some modifications. Briefly, liver samples (50 mg; wet weight) were homogenized in a tightly fitting glass-teflon homogenizer with about 500 μl of homogenizing buffer, buffer A (440 mM sucrose, 20 mM 3-(N-morpholino)propanesulfonic acid (MOPS), 1 mM ethylenediaminetetra-acetic acid (EDTA), pH 7.2, and 0.2 mM phenylmethylsufonyl fluoride). The sediment was homogenized in 500 μl of buffer B (500 mM NaCl, 10 mM MOPS, pH 7.2). After centrifugation at 20,000 g, 150 μl of the 1 M aminocaproic acid, 50 mM Bis-Tris–HCl buffer C (pH 7.0) and 20 μl of Brij 35 detergent solution was added. Mitochondrial pellets from mouse liver were suspended in an appropriate volume of buffer D (1 M 6-amiohexanoic acid, 50 mM Bis-Tris/HCl, pH 7.0), and the membrane proteins were solubilized by the addition of the indicated detergent and incubated for 10 minutes in ice. After centrifugation for 15 minutes at 100,000 rpm at 4°C, the supernatant was collected, and one-third of the final volume of the sample of 5% Serva Blue G dye in 1 M 6-amiohexanoic acid was added prior to loading. The following detergents were used (a) 3% (w/v) n-dodecyl β-d-maltoside (DDM) for preparation of native MRC complexes and (b) 4 g/g protein digitonin (DIG) to detect the supercomplexes.
MRC activity assays
Frozen liver tissues (50 to 70 mg) were homogenized with 15 vol. of 20 mmol/L KP buffer (K2HPO4), pH 7.4, and centrifuged at 800 g for 10 minutes. MRC enzymes and citrate synthase (CS) activities were measured in a DU-650 spectrophotometer (Beckman Instruments, Palo Alto, CA, USA). Incubation temperatures were 30°C for complexes I, II, III, V and CS, and 38°C for complex IV. Enzyme activities were performed in supernatants as described elsewhere , expressed as nM of substrate used per minute per mg protein and, to correct for the hepatic content of mitochondria, referred as a percentage of the specific activity of CS. Enzyme assays were performed in quintuplicate.
Assessment of full assembly of MRC complexes
MRC complexes from were isolated by one-dimensional BN-PAGE as described elsewhere . Following electrophoresis, proteins were transferred to a polyvinyl difluoride membrane (0.45-μm pore size) (Immobilon-P transfer membrane; Millipore Co., Bedford, MA, USA). Western blotting of these proteins was performed using primary antibodies against complex I subunits NDUFA6, NDUFA9, NDUFC2, NDUFV1, NDUFV2, NDUFS1, NDUFS7, NDUFS3, NDUFB6, NDUFB8, MTND6, complex II subunit 70 (SDHA), complex III subunit core 2 (UQCRC2), complex IV subunit COX-IV (COX4) and complex V subunit α (ATP5A1) (Molecular Probes Inc., Eugene, OR, USA) on blocking buffer for 2 h. After washing, blots were incubated for 1 h with peroxidase-conjugated antibody as a secondary antibody, prepared at a 1:5,000 dilution (Molecular Probes Inc.). Immunoreactive material was visualized by chemiluminescence (ECL, Western Blotting Detection; GE Healthcare, Madrid, Spain) according to the manufacturer’s instructions. The blot was finally exposed to Hyperfilm MP (Amersham, GE Healthcare).
Second-dimension electrophoresis for assessing complex subunits
For second-dimension SDS-PAGE, we followed the procedure previously described . Following electrophoresis, proteins were transferred to a polyvinyl difluoride membrane. Western blotting was performed using primary antibodies against complex I subunits NDUFA9, NDUFS3, NDUFB6 (Molecular Probes Inc.), NDUFV1, NDUFV2, NDUFS1, NDUFA6, MTND1, MTND2, MTND4, and MTND4L (Santa Cruz Biotechnology, Inc. Santa Cruz, CA, USA), complex II subunit SDHA, complex IV subunit COX4, complex V subunit ATP5A1 (Molecular Probes Inc.) in blocking buffer for 2 h. After washing, blots were treated as indicated above.
In-gel activity assays
MRC complexes were separated by one-dimensional BN-PAGE as described above and complex I in-gel activity was measured as described by Nijtmans et al. .
Quantitative real-time polymerase chain reaction
Primers used in quantitative real-time polymerase chain reaction
Mouse NDUFA9 (39 kDa)
5′-CAT TAC TGC AGA GCC ACT-3′
5′-ATC AGA CGA AGG TGC ATG AT-3′
Mouse NDUFB6 (17 kDa)
5′-ATA ACT TTT TGC GGG ACG GG-3′
5′-CAG GAA AAT CTC TCA TTG GTG-3′
Mouse NDUFS3 (30 kDa)
5′-AGG AAC ATG GCG GCG GCT GC-3′
5′-ATT TCA GCC ACA TAC TCT CC-3′
Mouse MTND1 (ND1)
5′-TGC ACC TAC CCT ATC ACT C-3′
5′-ATT GTT TGG GCT ACG GCT C-3′
Mouse MTND2 (ND2)
5′-ATG AGT AGG CCT GGA ATT C-3′
5′-ATC AGA AGT GGA ATG GGG C-3′
Mouse MTND4 (ND4)
5′-ATA ATT ATA ACT AGC TCA ATC TGC-3′
5′-TCG TAG TTG GAG TTT GCT AG-3′
Mouse MTND4L (ND4L)
5′-CTC ACC ATA GCC TTC TCA C-3′
5′-CGT AAT CTG TTC CGT ACG TG-3′
5′-ATG GAT GAC GAT ATC GCT G-3′
5′-GTT GGT AAC AAT GCC ATG TTC-3′
Mouse UQCRC1 (core 1)
5′-CCT ACA GCA CTC GAG AGC AC-3′
5′-AGG TGT GCC CTG GAA TGC TG-3′
Mouse UQCRC2 (core 2)
5′-TCC CTC AAA GTT GCC CC-3′
5′-GCA AGA CGT AGT AAA TGT GAG-3′
Mouse UQCRFS1 (FeS)
5′-GAT GTC AAG GTG CCC GAC TT-3′
5′-GAT CTC GAT CTT CGA CAT GG-3′
Mouse SDHA (70 kDa)
5′-CAT ACT GTT GCA GCA GCA CAG G-3′
5′-CCA CCA AAT GCA CGC TGA TA-3′
Mouse COX4 (COX IV)
5′-GAG CAC ATG GGA GTG TTG TG-3′
5′-CTG TCT TCC ATT CAT TGG TGC C-3′
5′-GAG ACT TTG GAG GCC AGC AAG-3′
5′-CCA AGG GTA GCT CAG TTT ATC AG-3′
5′-GAC AGT CCA AAG GGT TCC TCA G-3′
5′-CTG GAT GGT CCT CTT GAA GAA GGC-3′
5′-CTG CCA GCT TGG TGT CAT CAC-3′
5′-CTG ACT TCC TTG CAG CGA GC-3′
Human NDUFA9 (39 kDa)
5′- GAT TGT GGC CAC TGT GTT TGG-3′
5′-CTC CAG CTT CCT TGG ACA GT-3′
Human NDUFS3 (30 kDa)
5′-GTC AGA CCA CGG AAT GAT GTG-3′
5′-CTC AAA ACG GTT TTG CCG AG-3′
Human NDUFB6 (17 kDa)
5′-CTG CAG CAG CTG CGA GA-3′
5′-GAA TAA TCC AGA CAG GTA CAA G-3′
Human UQCRC1 (core I)
5′-GTT AGC CTG CTG GAC AAC G-3′
5′-CTT GAT GTA GTA AGC TGT GTG C-3′
Human UQCRC2 (core II)
5′-CCA AAT GGC TTG GTG ATT GC-3′
5′-CAT TAG AAT ATC AAC ATC ACC CC-3′
Human UQCRFS1 (FeS)
5′-GCC TGT GTT GGA CCT GAA G-3′
5′-CTG GAA ACG AAC TGG GTG AC-3′
Human COX4 (COX IV)
5′-CAG GGT ATT TAG CCT AGT TGG-3′
5′-CTC CTT GAA CTT AAT GCG ATA C-3′
Human SDHA (70 kDa)
5′-GGC TTG CGA GCT GCA TTT GG-3′
5′-GTT CTG CTA AAC GGC ATG CCA-3′
5′-GCA AAC TTG CTA GCA GTC CTC-3′
5′-GGT ACT GAG CCA ACT GCA TTC-3′
5′-GAG TGT GAG ATC ACC AAG CG-3′
5′-CAC ATG AGA CAC CAG TGC ATT C-3′
5′-CAG CAG AAT TGA GTC ACC CAA T-3′
5′-CAT ACT GCC CAC CAG AGA C-3′
Western blot analysis
MRC complexes were isolated by BN/SDS-PAGE as described above and the filters containing proteins were incubated with appropriated polyclonal antibodies against prohibitin, FOXRED1, and NDUFAF1 (Santa Cruz Biotechnology, Inc. Santa Cruz, CA, USA). Signals were detected using the ECL Western Blotting Detection Reagent (Amersham Ibérica, Madrid, Spain).
Measurement of total intracellular ATP content
HepG2 cells were grown to confluence in 75 cm2 tissue culture flasks (approximately 1.0×106 cells) and detached using trypsin. After washing in phosphate-buffered saline (PBS), cell pellets were divided into two portions for duplicate experiments. Before ATP determination, pellets were homogenized using perchloric acid and centrifuged ice cold at 15,000 g for 2 minutes to pellet insoluble materials. Supernatant was collected and 30 μl was added to a 96-well plate, then brought up to 50 μl with ATP assay buffer. ATP reaction mix and ATP measurement was performed using the ATP Colorimetric/Fluorometric Assay Kit (BioVision Research Products, Linda Vista, CA, USA) according to the manufacturer’s protocol.
Binding of [3H]PGZ to complex I subunits
A total of 100 μg of liver mitochondria isolated from a C57BL/6J mouse were incubated with 10 μM [3H]PGZ (PGZ 3H(G) hydrochloride; Hartmann Analytic, Grupo Taper SA, Alcobendas, Spain) at 27°C for 30 minutes. Afterwards, proteins were separated in parallel, simultaneously, on three one-dimensional BN-PAGE as described above. Following electrophoresis, proteins from one of these three gels were transferred to a polyvinyl difluoride membrane (0.45-μm pore size) (Immobilon-P transfer membrane; Millipore) as previously described . Western blotting of these proteins was performed using primary antibodies against complex I subunit 39 (NDUFA9), on blocking buffer for 2 h (Molecular Probes Inc.). The second gel was used to visualize radioactive subunits by autoradiography. The third gel was utilized to visualize complex I in-gel activity. Likewise, subunits of complex I were separated in parallel, simultaneously, on two SDS/BN-PAGE gels as described above. Following second-dimension electrophoresis, subunits from one of these two gels were transferred to a polyvinyl difluoride membrane as previously described and identified using antibodies against subunits NDUFA6, NDUFA9, NDUFB6, NDUFS1, NDUFV1, NDUFV2, and NDUFA2. The second gel was used to visualize radioactive subunits by autoradiography.
Liver mitochondria were isolated from liver homogenates by differential centrifugation as described elsewhere . Mitochondrial pellets were treated with 10 μM [3H]PGZ for 30 minutes and lysed in radioimmunoprecipitation assay (RIPA) buffer (20 mM Tris/HCl, pH 7.5; 150 mM NaCl; 2 mM EDTA; 1% sodium deoxycholate; 1% Triton X-100; 0.25% SDS). A total of 800 μg of mitochondrial lysates were incubated with 2 μg monoclonal antibody (NDUFA9; NDUFV2; NDUFS1; NDUFA6; NDUFB6; COX1; core 1) at 4°C overnight. Following incubation, the immune complexes were precipitated with 50 μl protein G (NDUFA9, NDUFB6, core 1, COX1) or protein A (NDUFA6, NDUFS1, NDUFV1) MicroBeads (Miltenyl Biotec Inc. Auburn, CA, USA) for 4 h on ice. Columns were placed in the magnetic field of the μMACS separator and rinsed with 200 μl PBS buffer (pH 7.5). Precipitates were placed onto the columns and let to run through. The columns were rinsed four times with 200 μl PBS buffer and once with 100 μl low salt buffer (20 mM Tris/HCl (pH 7.5)). Afterwards, 20 μl of preheated 95°C hot 1 × SDS gel loading buffer (50 mM Tris/HCl (pH 6.8); 50 mM dithiothreitol; 1% SDS; 0.005% bromophenol blue; 10% glycerol) was applied to the column and incubated for 5 minutes at room temperature. Following incubation, 50 μl of preheated 95°C hot SDS gel loading buffer was added and elute was collected as immunoprecipitate. This was separated into two aliquots. In one of these, the radioactivity of the sample was quantitated by scanning laser densitometry (Desk TopTM Scanner Plus, Amersham Pharmacia Biotech). The second aliquot was electrophoresed in a 10% acrylamide gel and transferred to a nitrocellulose membrane. Subunits were analyzed by western blot. In addition, the individual bands in the blot containing subunits were excised and their radioactivity was measured by liquid scintillation spectrophotometry.
Statistical analysis was performed using SPSS Statistical Software for Windows, version 9 (SPSS Inc. Chicago, IL, USA). The unpaired t test was used to assess the significance of differences between means. Each in vitro experimental condition was repeated three to four times. Quantitative data are expressed as the mean ± SD unless otherwise mentioned. P values <0.05 were considered significant.
Subunit α complex V
Blue native polyacrylamide gel electrophoresis
Estrogen related receptor α
Reduced flavin adenine dinucleotide
Mitochondrial respiratory chain
PPARγ coactivator 1α
Peroxisome proliferator-activated receptor γ
Subunit 70 kDa complex II
Subunit core 2 complex III.
This study was supported in part by grants from the ‘Fundación Mutua Madrileña’ (AP2884-2008; AP7257-2010; AP8540-2011) and from the ‘Fondo de Investigación Sanitaria’ (PI07/0052; PI10/0312), Spain.
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