Larval body patterning and apical organs are conserved in animal evolution
© Marlow et al.; licensee BioMed Central Ltd. 2014
Received: 4 November 2013
Accepted: 24 January 2014
Published: 29 January 2014
Planktonic ciliated larvae are characteristic for the life cycle of marine invertebrates. Their most prominent feature is the apical organ harboring sensory cells and neurons of largely undetermined function. An elucidation of the relationships between various forms of primary larvae and apical organs is key to understanding the evolution of animal life cycles. These relationships have remained enigmatic due to the scarcity of comparative molecular data.
To compare apical organs and larval body patterning, we have studied regionalization of the episphere, the upper hemisphere of the trochophore larva of the marine annelid Platynereis dumerilii. We examined the spatial distribution of transcription factors and of Wnt signaling components previously implicated in anterior neural development. Pharmacological activation of Wnt signaling with Gsk3β antagonists abolishes expression of apical markers, consistent with a repressive role of Wnt signaling in the specification of apical tissue. We refer to this Wnt-sensitive, six3- and foxq2-expressing part of the episphere as the ‘apical plate’. We also unraveled a molecular signature of the apical organ - devoid of six3 but expressing foxj, irx, nkx3 and hox - that is shared with other marine phyla including cnidarians. Finally, we characterized the cell types that form part of the apical organ by profiling by image registration, which allows parallel expression profiling of multiple cells. Besides the hox-expressing apical tuft cells, this revealed the presence of putative light- and mechanosensory as well as multiple peptidergic cell types that we compared to apical organ cell types of other animal phyla.
The similar formation of a six3+, foxq2+ apical plate, sensitive to Wnt activity and with an apical tuft in its six3-free center, is most parsimoniously explained by evolutionary conservation. We propose that a simple apical organ - comprising an apical tuft and a basal plexus innervated by sensory-neurosecretory apical plate cells - was present in the last common ancestors of cnidarians and bilaterians. One of its ancient functions would have been the control of metamorphosis. Various types of apical plate cells would then have subsequently been added to the apical organ in the divergent bilaterian lineages. Our findings support an ancient and common origin of primary ciliated larvae.
KeywordsApical-blastoporal axis Apical organ Body plan Larval evolution
Widespread occurrence of ciliated larvae, with similar overall body plans, has prompted radically different views regarding their evolutionary significance [16, 18–20]. While some authors consider them an ancient feature of the metazoan life cycle [16, 17, 19], others assume that they evolved multiple times independently [18, 20] (Figure 1). In the past two decades, studies of developmental genes involved in setting up the larval body plan have started to provide a new source of comparative data to resolve these conflicting views. These studies have revealed specific similarities such as the expression of the goosecoid and brachyury genes in foregut, midline and hindgut of lophotrochozoan and deuterostome larvae  (in a manner distinct from direct developers such as insects or vertebrates). Also, the ciliary bands characteristic for swimming larvae were found to specifically express otx and three conserved microRNAs in lophotrochozoans and deuterostomes, corroborating the homology of shared larval features [22, 23]. Conversely, dissimilar expression reported for nk2.1 and hnf6 in the apical plate of sea urchins and the episphere of abalone larvae  raised doubt regarding the common origin of similar shared larval features. However, these pioneering studies thus far relied on small gene sets.
More recently, an extended set of transcription factors has been shown to play a conserved role in patterning the larval body plan in hemichordate  and in sea urchin [26, 27], belonging to the deuterostomes, and in the sea anemone Nematostella vectensis, a cnidarian . These factors respond to the differential stabilization of β-catenin along the primary body axis, triggered by Wnt signaling [28, 29]. In particular, six3 and foxq2 have been shown to negatively respond to Wnt signaling in a complex sequence of patterning events [28, 29]. These factors specify apical territory around the apical pole [27, 30]. For the first time, the conserved regional expression of similar sets of transcription factors provides a molecular framework for the comparison of larval cell types and tissues and thus important clues to larval body plan and apical organ evolution.
In our current study, we investigated the apical patterning system in the marine annelid Platynereis dumerilii, a lophotrochozoan protostome with a canonical biphasic pelago-benthic life cycle (involving a pelagic phase, that is, swimming, primary larvae, and a benthic phase, that is, bottom-dwelling adults). We examined the set of transcription factors involved in apical patterning, including six3 and foxq2, in the episphere of the Platynereis trochophore larva and have shown that, as in deuterostomes and cnidarians, expression of these factors is sensitive to Wnt signaling. We found that the apical organ develops in a small central territory devoid of six3 expression, that instead expresses a number of other factors, many of which are found in the same location in other neuralians. By expression profiling, we molecularly characterized several cell types that form part of the apical organ in Platynereis, which we compared to apical organ cell types described for other animal groups.
Our results reveal that the larvae of cnidarians, protostomes and deuterostomes exhibit extensive similarity in the molecular topography of body regions around the apical organ, which we use to genetically define ‘apical plate’ and ‘apical organ’; the specification of these regions by a conserved apical signaling system; and the molecular fingerprint of a subset of apical organ cell types. These findings support homology of some primitive type of apical organ (and thus of swimming ciliated larvae) in Neuralia and are most consistent with an early and unique origin of animal larval forms (Figure 1A,B).
Molecular topography of the apical region
In the sea urchin [26, 27], the hemichordate Saccoglossus kowalevskii and the anthozoan cnidarian Nematostella[28, 30], expression of the transcription factors six3 and foxq2 demarcates the most apical body region. In Saccoglossus and Nematostella, the six3+, foxq2+ domain is peripherally overlapping with a ring of rx and bounded by even more peripheral rings of otx and otp expression [25, 28]. Likewise, fezf is expressed in the apical plate in sea urchin  and the hemichordate . We have previously shown that six3 is expressed in a large contiguous domain of the Platynereis episphere , peripherally overlapping with the expression of rx.
The Platynereis trochophore also shares expression features with cnidarian and deuterostome larvae specific to the developing apical organ. In sea urchin , Nematostella and Platynereis, the apical tuft and a girdle of cells surrounding the tuft are devoid of six3 expression (white dashed line in Figure 2B’ and compare to Figure 3 in Poustka et al.  and Figure 3d in Marlow et al. ). In Platynereis, the six3-free region extends into a dorsal patch of cells that becomes separated from the central patch by a band of six3+ cells (which includes the conspicuous crescent cells, see below). Inside the six3-free region, we detect restricted expression of several transcription factors (using the nuclei of the two apical tuft cells for reference; yellow circles in confocal apical views in Figure 2): otp (Figure 2G”; in addition to its ring-like expression, see above), nkx3 (Figure 2Q’), foxj (Figure 2K’) and hox1 (Figure 2L’). In addition, the irx gene is expressed in the dorsal patch of cells devoid of six3 expression (Figure 2M’). Notably, nkx3, foxj, the hox gene paralog anthox1 and irx are also expressed in the apical region devoid of six3 in Nematostella; foxj matches the region devoid of six3 expression in sea urchin  and apical irx expression has been documented in hemichordates, although broader than in annelids . An apical region devoid of six3 expression, ‘filled’ by the restricted expression of foxj, nkx3, hox and irx, thus represents a larval-specific transcription factor signature that molecularly defines the apical organ in several distinct, phylogenetically remote neuralian groups such as anemone and annelid (Figure 3) and lends strong support to its evolutionary conservation (see Discussion).
Ectopic Wnt signaling abolishes specification of the apical organ region
Morphological and molecular characterization of apical organ cell types
Light and electron optic studies have revealed cell types that make up the apical organs of different invertebrate larval groups [11, 52–54]. In Platynereis, the apical tuft was visible by 16 hpf (cf. trpV channel expression at 24 hpf, Figure 2P) and other apical organ cells were likewise post-mitotic by 24 hpf (Figure 6A), expressing markers indicative of neuronal differentiation. At 30 hpf, several apical cell types were fully morphologically differentiated (Figure 6B; ). We found that the apical tuft was formed by two basket-shaped cells with intracellular tubulin support structures (Figure 6C); cells with a very similar morphology, referred to as ampullary cells, have previously been described in mollusk larvae . These cells persisted deep in the medial brain at later stages in the center of a massive commissural and neurosecretory neuropil (Figure 6J), and may thus represent a structural organizing center for the juvenile nervous system, as suggested for other polychaete larvae . Dorsal to the ampullary tuft cells, we found another set of large cells with multiple motile cilia in a crescent-moon shape, known as crescent cells (Figure 6D). Two serotonergic cells have also been found in the apical organ region by 30 hpf . Closest to the tuft was a serotonergic interneuron (white arrow, Figure 6D) lacking sensory dendrites. This cell was located deep in the epithelium, adjacent to an assembly of previously described sensory-neurosecretory flask-shaped cells (Figure 6E) that, morphologically, resemble chemosensory cells  (called parampullary cells in mollusks ). More ventral to the parampullary cells, we detected a median pair of cells bearing short, stiff and curly sensory cilia resembling mechanoreceptors (Figure 6F).
These distinctive morphologies, in conjunction with the recently established Profiling by Image Registration (PrImR) technique, enabled us to assign a molecular fingerprint to these cells, providing them with unique molecular identities . PrImR utilizes the stereotyped development of the Platynereis axonal scaffold to generate in silico alignments of mRNA in situ expression patterns, and allows single cell co-expression analyses to be conducted . Of a collection of 140 genes currently available for PrImR single cell co-expression analysis, 29 were differentially expressed in cells of the apical organ region in the 48 hpf larva. Additional file 1: Figure S5 details the PrImR-based co-expression analysis for the above-mentioned morphologically identifiable cells, namely the ventral-most serotoninergic cell (Additional file 1: Figure S5A), the parampullary sensory-neurosecretory cells (Additional file 1: Figure S5B-E), the ampullary tuft cell (Additional file 1: Figure S4F), the crescent cells (Additional file 1: Figure S5G) and the pair of putative mechanoreceptors (Additional file 1: Figure S5H).
PrImR revealed unique sets of genes expressed by each of these cell types, in line with their specialized sensory-neurosecretory and neuronal characteristics. For example, as determined previously, the flask-shaped parampullary cells expresses otp (Figure 6G; Additional file 1: Figure S5B-E; and see above), mir-7 (Figure 6H) and prohormone convertase 2 (phc2) . Beyond that, PrImR allowed cellular allocation of transcripts encoding neuropeptide precursors for DLamide, FMRFamide and WLDamide (Figure 6I), consistent with a conserved role of otp in specifying different types of peptidergic cells . These three neuropeptides mediate opposing effects on locomotor behavior with DLamide and FMRFamide exposure leading to an increase and WLDamide leading to a decrease in ciliary beating frequency . The otp + peptidergic cells also expressed MIP, the recently described settlement-inducing neuropeptide . In addition, we found that all otp + sensory-neurosecretory cells were positive for the newly identified peropsin gene (Figure 6K), an opsin-related photopigment that may function as photopigment or photoisomerase , indicating that these cells (or adjacent cells) are light-sensitive. Complementing this, we observed that the previously characterized c-opsin1, an ortholog of rhodopsin and other c-opsins, was expressed in cells around the apical organ (Figure 6L). As c-opsin1 expression is difficult to score in 48 hpf larvae (the stage for which the PrImR resource is available), we were not able to further characterize the c-opsin1+ cells. One of the otp + cells was demarcated by the expression of lmx1ab (a terminal selector gene for serotoninergic neurons ) and by position correlates to the serotonergic interneuron (Additional file 1: Figure S5A; Figure 6I). The ampullary apical tuft cells (Additional file 1: Figure S4F) specifically expressed hox1 (Figure 2L) and a trpV channel previously implicated to serve mechanosensory roles in other protostomes (Figure 2P) , indicative of multiple sensory modalities. Finally, and consistent with their specialized morphology, the two putative mechanosensory cells (Additional file 1: Figure S5H) expressed miR-183 (Additional file 1: Figure S1), a conserved microRNA that demarcates chemo- and mechanosensory cells across bilaterians [22, 59, 60]. Furthermore, they were the only cells to express otx (compare Figure 2E’).
Hierarchical clustering reveals distinct groups of apical organ cells
In line with our observation that at 24 hpf the apical organ region including the tuft cells was devoid of six3 expression, the tuft cells themselves and the directly ventrally adjacent sensory-neurosecretory and serotoninergic cells were six3-negative at 48 hpf. By contrast, the crescent and mechanoreceptor cells expressed six3 and thus, by molecular identity, appear to represent differentiated cell types of the surrounding apical plate. Hierarchical clustering based on the 48 hpf PrImR data (Figure 6M) supports this distinction: one cluster comprised the flask-shaped sensory-neurosecretory cells and the adjacent serotoninergic cell, which, in addition to the absence of six3 and presence of otp, miR-7 and phc2 (see above), expressed carboxypeptidase-E (cpe) and other genes not directly linked to neurosecretion, such as smad2/3. This cluster corresponded to the otp + cells devoid of six3 expression at 24 hpf (see above). Another well-supported cluster comprised the crescent cells and the putative mechanosensory cells, which, in addition to six3, expressed the microRNA miR-29 (Figure 6H), the ctbl1 and bZIP.TF genes encoding transcription factors of unknown function, and tektin-2, a structural component of microtubules . Note that in our clustering, absence and presence of six3 and foxq2 strictly correlated, indicative of co-regulation  (Figure 6M).
The ampullary tuft cells devoid of six3 (and foxq2) exhibited a more separate identity with equal distance to either cluster. With the six + (and foxq2+) mechanosensory and crescent cells, they shared expression of foxJ, which plays a role in the formation of long motile cilia , and of three microRNAs of the ‘ciliary’ group that have previously been found to demarcate locomotory ciliary bands across Bilateria . They shared the expression of the transcription chx10 with the peptidergic cells (Figure 6M).
Evolutionary conservation of apical patterning
Notably, although the six3+, foxq2+, rx+, fezf+, nk2.1, onecut + apical plate, as defined here, is a larval character, the same genes are also co-expressed in bilaterian phyla that have lost the primary larva, such as insects and vertebrates, where they specify anterior brain regions [34, 91, 92]. This is consistent with previous observations that, after Platynereis metamorphosis, apical plate markers remain expressed and demarcate the developing cerebral ganglia of the adult nervous system . Therefore, while the stereotypical, partially overlapping co-expression of the above-mentioned genes can be used to topographically ‘align’ larval body plans, it is not a unique feature of primary ciliated larvae.
A universal molecular definition of the apical organ region
The apical organ develops in the center of the apical plate. In annelid, sea urchin  and sea anemone [28, 30], this apical organ region is specifically excluded from the region of six3 expression (in annelid (Figure 3A) and cnidarian (Figure 3C) ). Instead, it expresses the transcription factors foxj (in sea anemone  and sea urchin ), nkx3 (in annelid (Figure 2Q) and sea anemone ), irx (in annelid (Figure 2M) and sea anemone ) and a hox paralog (in annelid (Figure 2L), mollusk  and sea anemone ). Note that all of these factors are also expressed elsewhere; for example, in Platynereis, foxj is expressed in other six3+ ciliated apical plate cells and, more generally, in ciliary bands; nkx3 is expressed in other apical plate cells (our data) and in segmented mesoderm  and hox1 is expressed in the second larval segment  - nevertheless, the recurrent appearance of these factors in the apical organ region across neuralians appears highly significant and we propose that it reflects the evolutionary conservation of apical organ cell types (see below). In addition, apical organ formation appears to similarly depend on local FGF signaling, as shown for sea anemone  and as suggested by localization of the fgf receptor to the apical plate, in sea urchin  and annelid (Figure 2N). Also, the common localization of Tgfβ signaling inhibitor noggin to the apical organ, as seen in sea anemone , sea urchin and in Platynereis, is indicative of conserved signaling events. Together, these transcription factors and signaling molecules provide a highly characteristic molecular signature for the apical organ region (and the cell types therein, see below). Given that the establishment of the six3-‘hole’ spatially correlates with, and has been functionally linked to, the formation of the apical tuft (see below), we consider this signature a characteristic feature of primary larvae and its apparent evolutionary conservation lends strong support to the notion that these larvae represent an ancient feature of the metazoan life cycle (Figure 1A and see above). It will be interesting to determine to what extent this signature or parts of this signature are present in groups that have lost primary larvae; given the spotted appearance of irx, nkx3, hox and foxj expression in the apical organ region it is likely that any conservation of this signature at adult stages would relate to the persistence of ‘apical’ cell types throughout the life cycle.
It should be stressed that the hox genes expressed in the apical organ in cnidarian, mollusk and annelid represent different paralogs of the Hox cluster. Anthox1, which demarcates the apical organ in sea anemone, is a ‘posterior’ hox gene ; Lox5, Lox4 and Lox2, which are expressed in different cells of the apical organ of the snail trochophore , belong to the middle part of the cluster; and hox1, that we find expressed in the Platynereis apical organ, is an ‘anterior’ hox gene. The utilization of different hox paralogs in the apical organ can be explained if one assumes that hox expression in apical organ cells is older than the hox cluster itself and already occurred at the times when a single ur-hox/parahox gene existed . Then, concomitant with subsequent duplication events, the expression of different hox paralogs would have been lost in divergent evolutionary lines. Alternately, the hox paralogs utilized in the apical organ may have been exchanged over time. Additional studies examining the downstream targets of hox expression will be useful in distinguishing between these scenarios.
The apical tuft - ancient nucleation center of apical organs
If the neuralian ancestors specifically expressed the molecular signature defined above in selected cells of the apical organ region, what cell types did the partaking genes specify and what can we learn about the ancestral composition and function of the neuralian apical organ? Except for Platynereis, cellular resolution molecular fingerprints are not yet available in marine primary larvae, limiting the extent of interphyletic comparison of specific cell types. However, limited molecular evidence is available and adds to a rich body of morphological evidence. The first and most obvious candidates for conserved apical organ cell types are the apical tuft cells that are widespread in Neuralia [2, 11, 85, 98]. In Platynereis, the apical tuft cell exhibits a basket-shaped morphology with characteristic intracellular support structures resembling those of ampullary tuft cells in mollusks  and stands out with the specific expression of hox1. In the snail Gibbula, expression of Lox4 is confined to ‘two cilia-bearing cells of the apical organ’ ; it is as yet unclear how these relate to the ampullary tuft cells. In sea anemone, injection of anthox1 morpholino results in planulae lacking the apical tuft , corroborating a link between hox expression and tuft formation. Given that anthox1 knockdown also abolishes the ‘hole’ in six3 expression, one might speculate that the formation of an apical tuft involving restricted hox expression, FGF signaling and apical down-regulation of six3 represents the conserved core of apical organs in Neuralia (see above). This notion would imply, however, a transition from monociliated to multiciliated tuft cells: while the Platynereis apical tuft cell bears multiple long motile cilia, those of some other spiralian and of deuterostome larvae are monociliated ; likewise, the cnidarian apical tuft is composed of many cilia that each emerge from single monociliated cells . These long cilia perform a mechanosensory function during sweeping behavior of the benthos [99, 100]. In the absence of cell type-specific expression data from other neruralian larvae, the evolutionary relationship of the various tuft cells remains speculative; future studies will further clarify their evolutionary relationships within neuralians.
The apical plexus - a conserved sensory-neurosecretory release site for the control of body physiology and metamorphosis
Besides the apical tuft, the apical plexus directly underneath the apical tuft may belong to the ground pattern of apical organ structures in Neuralia, as it is commonly innervated by flask-shaped, neuropeptidergic sensory-neurosecretory cells from the apical plate or apical organ proper - in mollusks [101, 102], sea urchins , hemichordates  and, likewise, cnidarians [85, 103–107]. Among the neuropeptides secreted by these cells into the apical plexus may be two ancient types of short amidated peptides, Wamides and R(F/Y)amides, recently proposed to predate neuralians . In cnidarians, RFamidergic sensory neurons from ‘anterior’ larval regions project into the apical plexus [85, 105, 106, 109]. Likewise, RFamidergic cells populate the apical plate and organ in annelids (as shown here and in [12, 110]), mollusks [101, 102], polyclad flatworms  and phoronids . Wamides have been implicated in the control of larval settlement in cnidarians [112, 113] and, recently, in annelids  and are likewise released from the apical plexus. In the late planula larva of the hydrozoan Hydractinia, GLWamide-positive sensory cells that innervate the apical plexus populate a belt around the apical pole , which, by position, forms part of the apical plate; MIP, a related Wamide neuropeptide that induces larval settlement in annelids, is secreted into the apical plexus by apical organ cells .
In Platynereis, the transcription factor otp demarcates the neuropeptidergic sensory-neurosecretory cells in apical organ (Figure 2G,G’ ,G” and ). Since otp + cells also exist in the apical organ of the snail Patella and are overlapping with six3 expression in the apical plate of the late Nematostella planula , we hypothesize that otp + sensory-neurosecretory cells projecting to the apical plexus formed part of the apical plate in neuralian ancestors and that subsets of these cells became part of the apical organ in the bilaterian lineage. Another gene demarcating the neuropeptidergic sensory-neurosecretory cells, among other cells, is nkx3. The apparent deep conservation of nkx3 expression in apical organs (see above) suggests an ancient role of nkx3 in the specification of these apical neurons; to find out, a thorough investigation of nkx3+ cells in other apical organs, for example in the cnidarian planula, will be rewarding.
Besides the neuropeptidergic cells, apical serotoninergic cells represent a third type of neurosecretory cell projecting into the apical plexus. They exist in mollusks , echinoderms , enteropneusts  and other bilaterian phyla . Serotonergic cells are also enriched in apical body regions of the hydrozoan cnidarian Phialidium gregarium, where the release of serotonin has been reported to trigger larval settlement . The molecular identity of serotoninergic apical cells is beginning to be elucidated: the 5HT cell included in our PrImR analysis appears to specifically express lmx1ab, a LIM homeodomain factor implicated in serotoninergic specification in nematodes , and nk2.1, demarcating serotoninergic cells in sea urchin  and, possibly, in the Ptychodera tornaria . Likewise, fezf has been proposed to correlate with serotoninergic fate in the sea urchin . Further genetic studies on the serotonergic system in various marine larvae will be needed to resolve this issue.
Ambient light detection
Finally, our study provides strong evidence for photosensitivity being an ancient feature of apical organs. Remarkably, the opsins identified in cells in and around the apical organ in Platynereis (Figure 6K,L), and also in Terebratalia transversa and Nematostella vectensis, all fall within the peropsin/ciliary opsin families. These observations indicate that apical organs evolved as multimodal sensory structures, of which photosensitivity formed a key component.
‘Minimally indirect development’ links apical patterning of larval and adult stages
The continuous deployment of the apical patterning system at larval and adult stages and the persistence of some apical plate and organ cell types into post-metamorphic stages would suggest that a gradual type of metamorphosis (where the bulk of tissues persist, see below) is more ancient than the ‘catastrophic’ mode of metamorphosis dubbed maximal indirect development . We refer to such a biphasic life cycle, with gradual and limited metamorphosis in which larval neural structures are incorporated into the adult nervous system, as ‘minimally indirect development.’ Illustrating this, the Platynereis apical organ tuft cell appears to form a ‘nucleation center’ around which the brain is organized (Figure 6I), and the larval axons pioneer the tracts and nerves of the later nervous system [31, 54]. It is possible that the eumetazoan common ancestor showed minimally indirect development with a larval stage resembling the primary ciliary larvae of modern marine bilaterians and cnidarians.
We have investigated regionalization of the larval episphere, the effects of ectopic activation of Wnt signaling on apical patterning, and the molecular fingerprint of apical cell types in the marine annelid Platynereis dumerilii. Comparing our findings to those in other marine larvae, we present a core set of characteristics common to primary ciliated larvae in bilaterians and cnidarians. All larvae develop an apical plate that we define by a combination of transcription factors most prominently involving six3 and foxq2. Expression of these factors and formation of the apical plate is sensitive to Wnt signaling activity. Finally, a conspicuous apical tuft forms within a central six3-free territory within the apical plate. These similarities are most parsimoniously explained by common origin. We accordingly propose that the last common ancestor of bilaterians and cnidarians developed via primary larvae that possessed an apical tuft as part of a simple apical organ. A basal plexus may have formed directly underneath the apical organ, which was innervated by sensory-neurosecretory apical plate cells. We hypothesize that an ancient function of the apical organ was the control of metamorphosis and opsin-based ambient light perception. Various types of additional apical plate cells would then have subsequently been recruited to form part of the apical organ in the divergent bilaterian lineages. Our findings support an ancient and common origin of primary ciliated larvae.
Isolation of Platynereisgenes and sequence analysis
Partial sequences for pdu-fgfr, pdu-foxq2, pdu-irx, pdu-foxJ1, pdu-hnf6, pdu-wnt4, pdu-frizzled5/8, pdu-sfrp1/5 and pdu-peropsin were assembled from Platynereis transcriptome and genome resources, amplified with specific primers, and extended with rapid amplification of cDNA ends PCR. Pdu-CTBL1, Pdu-bZIP-TF, Pdu-tektin-2, Pdu-spondin, Pdu-gbrl, Pdu-cpe, Pdu-smad2/3 and Pdu-klf2/4 were characterized during a whole mount in situ hybridization screen from expressed sequence tag clones (RT and DA, unpublished). The GenBank accession numbers for peropsin, foxj, fezf, onecut, fgfR, noggin, foxq2, frizzled4, frizzled5/8, frizzled9/10 and sfrp1/5 are [GenBank:KF844232] to [GenBank:KF844242] respectively. Accession numbers for ctbl1, spondin, gbr1, cpe, ces2 and klf are [GenBank:KF835846] to [GenBank:KF835851] respectively.
In situhybridizations and immunostainings
For in situ hybridizations at early stages, Platynereis larvae were fixed in 4% PFA, 0.1 M MOPS, 2 mM EGTA, 1 μM MgSO4 and 0.1% Tween-20, for 4 to 6 hours at 4°C, then rinsed in PTW and ice cold methanol, followed by storage in methanol at -20C.
In situ hybridization for early Platynereis larvae were performed as in [118, 119] with the following modifications. Embryos were digested with 0.1 mg/mL proteinaseK for 30 seconds (Merck 7066304, Darmstadt, Germany). Following hybridization, 0.5 × SSC washes were replaced by 0.15 × SSC washes for 15 rather than 30 minutes. In situ hybridizations were performed for 48 hpf Platynereis and microRNA as previously published [22, 118]. The axonal scaffold was counterstained with an antibody against tyrosinated or acetylated-tubulin (1:500, Sigma T9028 and T6793). Immunostainings were performed as described  with the following primary antibodies: mouse anti-acetylated tubulin (1:500, Sigma T6793), rabbit anti-serotonin (1:500, ImmunoStar 20080) and rabbit anti-FMRF (1:200, Phoenix Pharmaceuticals, H-047-29).
For the staining of mitotic cells, Platynereis larvae were incubated in 10 μM EdU (Click-iT EdU Imaging Kit, Invitrogen, C10340) from 22 to 24 hpf; EdU incorporation was detected after the incubation with secondary antibodies, following manufacturer instructions.
Alsterpaullone and azakenpaullone treatments
Platynereis larvae were incubated from 12 to 24 hpf in five different concentrations of azakenpaullone (0.1 to 10 μM) in 0.5% dimethyl sulfoxide (DMSO) in filtered seawater. The number of embryos displaying wild-type, reduced or expanded expression patterns for episphere molecular markers were assessed from two different biological replicates (numbers of embryos displaying each pattern are indicated in Figure 5). As most genes assayed were significantly affected at 1 and 5 μM concentrations, we conducted washout experiments at these concentrations. Larvae were exposed to azakenpaullone from 12 to 24 hpf and subsequently washed out of the pharmacological treatment into 0.5% DMSO and incubated in 0.5% DMSO from 24 to 30 hpf. Controls were maintained in 0.5% DMSO from 12 to 24 hpf and washed into a new 0.5% DMSO treatment from 24 to 30 hpf. Embryos were examined and assessed in relation to control-treated embryos. Effects of alsterpaullone were also assayed, compared to those of azakenpaullone, and found to be similar to azakenpaullone treatments (Additional file 1: Figure S2).
Microscopy and image processing
We used reflection microscopy  to acquire confocal images of in situ hybridization stainings. Fluorescent signals in Figure 2Q’ , Figure 4C and Figure 4C’ were obtained using the fluorescent signal emitted from NBT/BCIP precipitate, an alternate method to reflection microscopy. Confocal stacks were taken on a Leica TCS SPE with a 40× oil immersion objective. Images were processed with ImageJ, utilizing either brightest point or average intensity settings to generate projections. Subsequently, images were cropped and processed in Photoshop, Adobe, San Jose, California, USA; brightness and contrast were adjusted equally across the entire image.
For PrImR, average expression patterns at 48 hpf were obtained after image registration of in situ confocal scans on a common reference axonal scaffold, as described in .
Molecular fingerprint analysis
Morphologically distinct apical organ cell types were identified by analyzing immunostained larvae at early stages (24 and 30 hpf).These cells were then located in 48 hpf larvae for the gene expression analysis with PrImR. The co-localization between two average gene expression patterns was inspected and visualized using the ‘Colocalization highlighter’ plugin in ImageJ.
Whenever a PrImR average expression pattern was not available for the gene and/or the stage of interest, specimens stained with the gene of interest and tyrosinated tubulin were inspected under fluorescence microscopy.
Hierarchical clustering of molecular fingerprints was carried in R (the R Project for Statistical Computing) from the dataset in Additional file 1: Figure S5, using Pearson correlation and average linkage.
Phylogenetic analyses for gene orthologies
Platynereis dumerilii gene coding sequences used in this study were isolated as described above. Sequence data from the lophotrochozoans Lottia gigantea and Capitella teleta and the cnidarian Nematostella vectensis were identified on their respective JGI genome portal webservers. Additional sequences used in the analyses were downloaded from Treefam . Multiple alignments of predicted proteins were generated with MUSCLE using the default settings  and were subsequently inspected and corrected by eye. Full alignments were trimmed using G-blocks  and were run through ProtTest using the default settings to determine the optimum evolutionary model for phylogenetic analyses . Neighbor joining trees were constructed using MEGA  and maximum likelihood analyses were conducted using PhyML  with the amino acid substitution models specified from ProtTest. Phylogenetic trees are available in Additional file 2.
fibroblast growth factor
polymerase chain reaction
profiling by image registration
transforming growth factor.
We thank all of the members of the Arendt group for useful discussion and F. Christodoulou and G. Jekely for contributing averaged expression patterns to the PrImR database. We also thank Antje Fischer for sharing preliminary expression data for nkx3.1 in the Platynereis brain. We are also very grateful to Dr. Claus Nielsen for contributing his original drawings that have greatly improved the quality of Figure 1 and also thank him and two anonymous reviewers for their improvement of the manuscript. The work was supported by: the European Molecular Biology Laboratory (RT, TL, DA); the EMBL International PhD Programme (PRS, AL); and the EMBL Interdisciplinary Postdoc Programme under Marie Curie Actions COFUND (HM) and by the European Union’s Seventh Framework Programme project: ERCEA-AdG-2011-294810_‘BrainEvoDevo’.
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