Host-driven diversification of gall-inducing Acacia thrips and the aridification of Australia
© McLeish et al; licensee BioMed Central Ltd. 2007
Received: 25 August 2006
Accepted: 26 January 2007
Published: 26 January 2007
Insects that feed on plants contribute greatly to the generation of biodiversity. Hypotheses explaining rate increases in phytophagous insect diversification and mechanisms driving speciation in such specialists remain vexing despite considerable attention. The proliferation of plant-feeding insects and their hosts are expected to broadly parallel one another where climate change over geological timescales imposes consequences for the diversification of flora and fauna via habitat modification. This work uses a phylogenetic approach to investigate the premise that the aridification of Australia, and subsequent expansion and modification of arid-adapted host flora, has implications for the diversification of insects that specialise on them.
Likelihood ratio tests indicated the possibility of hard molecular polytomies within two co-radiating gall-inducing species complexes specialising on the same set of host species. Significant tree asymmetry is indicated at a branch adjacent to an inferred transition to a Plurinerves ancestral host species. Lineage by time diversification plots indicate gall-thrips that specialise on Plurinerves hosts differentially experienced an explosive period of speciation contemporaneous with climatic cycling during the Quaternary period. Chronological analyses indicated that the approximate age of origin of gall-inducing thrips on Acacia might be as recent as 10 million years ago during the Miocene, as truly arid landscapes first developed in Australia.
Host-plant diversification and spatial heterogeneity of hosts have increased the potential for specialisation, resource partitioning, and unoccupied ecological niche availability for gall-thrips on Australian Acacia.
Determining the driving forces behind the diversification of phytophagous insects remains a central challenge to biology. Increases in insect species numbers can be expressed as an outcome of their feeding on plants [1, 2]. The evolution of this type of ecological relationship between specialists and patterns of host-plant affiliation has been well documented [3–12]. The majority of phytophagous insect species are specifically associated with, and generally show preference for, single plant families, and this relationship is presumed to be evolutionarily conservative. This association is displayed in the narrow food specialisation and life habits of the majority of insect species. The mechanisms responsible for the generation of diversity in plant-insect systems are usually thought to result from cospeciation, or frequent host switches followed by specialisation. Host range expansion is believed to provide opportunities for vicariance, and the subsequent effects of selection or drift fuels the diversification of specialists . This is in line with predictions that reciprocal radiations should occur for some insect-plant associations [4, 14, 15] where the proliferation and distribution of endoparasites and their hosts are expected to emulate one another.
The evolution of endemic biodiversity in contemporary Australia has primarily arisen over a period of increasing aridity during the late Tertiary period [16–20]. Truly arid landscapes appear some time towards the end of the Miocene, and more recently, dramatic changes to the terrestrial environment occurred during the Quaternary period, when glacial-interglacial cycling drove a rapid increase in desertification. The aridification of Australia modified habitat enhancing the evolution of flora, including sclerophylly. Range expansion and contraction of sclerophylly during glacial cycling [21–23] would be expected to provide opportunity for explosive speciation [15–17, 24–31]. However, the proposal that faunal and floral diversification in Australia has been partly driven by climatic glacial-interglacial cycling effects over the last 2–3 million years remains a point of conjecture and is difficult to test .
Global patterns of gall-inducing species richness are believed to increase from mesic to xeric environments across transitional vegetation gradients from mesophytic to sclerophyllous plants [29, 33–36]. Species-specific gall induction by thrips (Thysanoptera: Phlaeothripidae) on closely related desert Acacia (Leguminosae) trees has likely evolved as a consequence of the developing arid landscape [27, 37–39]. Acacia is a prominent endemic Australian xeromorphic plant genus  and the most speciose plant genus on the continent, distributed throughout arid and semi-arid regions that cover over 70% of the Australian landmass [26, 40]. The presence of sclerophyllous phyllodes (leaf-like modified petiole) is characteristic of Acacia on which gall-thrips induce galls  and are an adaptation to conserve water and to low nutrient environments [18, 42–44]. Acacia population subdivision is partly determined by soil type mosaics [45–48] and this patchwork of nutrient poor sand dunes, sand plains and rocky ranges provided the sclerophylly habitat and opportunity for speciation [16, 24, 32]. Patchy distributions of Acacia are expected to contribute to heightened extinction and speciation rates . This type of environmental heterogeneity can potentially also provide a foundation for increased specialisation and resource partitioning .
Acacia gall-thrips are a monophyletic group, distinct from other gall-inducing groups in Australia, implying that gall induction had a single origin in this case . Acacia gall-thrips have likely undergone diversification as a result of cospeciation, highly restricted host-plant switching, or both processes [52, 53]. The beginning of extensive aridification in Australia dates from the Late Miocene approximately 10–15 million years ago. The origin of Acacia gall-thrips likely arose in conjunction with the drying of Australia during this period . In this study, we argue that host-specific gall-inducing thrips have diversified in close accordance with evolution of highly-related host Acacia in the subgenus Phyllodineae (DC.) Seringe, comprising more than 960 species largely confined to the Australian arid-zone . Using a phylogenetic approach, we demonstrate that net increases in gall-thrips diversification are closely linked with host range expansion and aridification in Australia, particularly during the Quaternary period.
Gall-thrips diversity partly depends on a highly specialised affiliation with host Acacia species, where all described species are monophagous . Galls are paramount in providing food, shelter, and a vessel for reproduction. The development and growth of galls has been widely correlated with the feeding activity and nutritional requirements of the galler . Single females, or a female-male pair, induce a gall on the phyllode of a specific host plant and become fully enclosed within a few days after initiation. Extreme host specificity is evident in the morphologies of galls induced on different host species, and are invariant in appearance given any particular host species affiliation. Gall morphology amongst a species takes a generalised form of either an elongate or pouched structure, with more discrete characteristics evident for galls induced on specific hosts . Elongate and pouched gall-types vary in appearance by the presence/absence of either spiky protrusions or prominent longitudinal ridges. Gall induction by founders is only permitted when new phyllode growth is available for inoculation. This event is usually dictated by local, infrequent, and unpredictable arid-zone rainfall patterns.
Sections Plurinerves and Juliflorae of the subgenus Phyllodineae, host to all but one gall-thrips species, do not appear to represent monophyletic groups but remain closely related to one another [54, 55]. Species within these sections are believed to be relatively young compared to other sections in the subgenus Phyllodineae and more susceptible to speciation during the Pleistocene climatic cycling . Semi-arid transitional climatic zones on the periphery of the interior arid zone have been proposed as having been particularly favourable for floral [21, 31, 32] and faunal [23, 56, 57] speciation. The lack of sequence divergence within the chloroplast genome of host Acacia and their close relatives, as well as evidence of a rapid morphological radiation among Acacia in subgenus Phyllodineae, is symptomatic of a relatively young group . Speciation in the Plurinerves is believed to have occurred over a relatively short period as widespread aridity developed in the late Tertiary and early Quaternary periods [16, 17] and that tropical section Juliflorae is more ancient than the Plurinerves section .
The addition of 29 undescribed taxa are added to all described Acacia gall-thrips species  to infer diversification hypotheses. These new taxa have previously been considered cryptic species (also referred to hereafter as morphotypes) and more recently believed to be groups of monophagous sister-species [52, 53, 59]. Recent work has raised one of these morphotypes to species status . The extension of the gall-thrips phylogeny with the addition of these taxa is now believed to include a great majority of known types, although formal species delimitation is yet to be undertaken. We approached this study in two steps. First, to infer biogeographical processes acting on the genesis of diversity in gall-thrips/Acacia interactions, we made the assumption that gall-thrips diversification and host radiations were closely linked, and used species-level phylogenetic analyses and penalised likelihood approaches to date gall-thrips lineage diversification. Second, to address hypotheses of rate increase in gall-thrips diversification, expected as an outcome of Acacia radiations, we used likelihood ratio tests to identify late-branching molecular polytomies correlated with periods of explosive radiation, and tree asymmetry methods to identify rate changes.
The monophyly of the K. waterhousei complex is apparently violated by K. rodwayi, K. habrus, and K. intermedius; similarly, K. rugosus is not monophyletic due to the presence of K. maslini and K. nicolsoni. The inclusion of these described species within the complexes is suspected to result from phylogenetic uncertainty, an artifact of host switching [52, 53], and that the undescribed taxa in each complex are species. Genetic divergence and substantial variation in gall structure amongst each of the complex members suggests that at least some of the group comprises species. Unpublished work (MJ McLeish & BJ Crespi) indicates COI pairwise uncorrected "p" distances between a majority of species complex taxa is similar to those between described species. Although such distances should be treated cautiously when inferring species-level divergence, in one case at least the divergence has been shown to be at the species level. For example, the recent elevation of a K. rugosus host race inhabiting Acacia papyrocarpa (Benth) to species status, K. nicolsoni , is consistent with the complexes comprising putative species. The genetic distances between taxa belonging to each of the species complexes, host species-specific gall morphology, and cospeciation  between gall-thrips and Acacia is consistent with monophagy operating for these morphotypes. Regardless of the presence of some phylogenetic uncertainty, the high support for each clade that includes either the K. rugosus or K. waterhousei complexes provides the ability to assess the validity of a hypothesized increase in the rate of diversification for each of them, as the clades are expected to have co-radiated and therefore have radiated contemporaneously.
Penalised likelihood analysis using our Bayesian inference revealed contemporaneous radiations of two gall-thrips clades, one comprising the K. rugosus complex and the other K. waterhousei, Kladothrips intermedius (Bagnall), and Kladothrips habrus (Mound) complexes. We explored outcomes of r8s tests by assigning different combinations of fixed and constrained ages to the root and an internal node corresponding to the most recent common ancestor of the clade containing the K. rugosus group (Table 2), to assess the ratio (or fit) between each of our calibration priors. When the root node was fixed at 25 million years ago (mya), approximating the appearance of fossil Acacia pollen in Australia, the internal node corresponding to the presumed period of rapid speciation of Plurinerves, was dated at nearly 9 mya. This date approaches the period when arid-adapted Acacia are believed to have originated and therefore 25 mya was regarded as an upper limit for the age of the root node.
Fixing the age of the root node at 15 mya, and then constraining the age between 10–15 mya in two separate tests resulted in the age of the appearance of the common ancestor of the K. rugosus and K. waterhousei complexes being dated at between 3.3 and 5.3 mya (Table 2). Furthermore, we enforced a fixed divergence time of 5 mya at this node in one analysis and constrained this point between 2 mya and 5 mya in another test. The root node date estimates were 9.3 mya and 13.7 mya respectively (Table 2). Therefore, the ratio of the age of the root node and the common ancestor of the K. rugosus and K. waterhousei complexes were consistent with one another between the independent analyses.
To generate our chronogram (Figure 2), we used a gamma shape distribution value of 0.5316 generated using Modeltest 3.0 . The age at the root and at the common ancestor of the K. rugosus complex was constrained between 10 and 15 mya and 2 and 5 mya respectively. The cross-validation analysis yielded a smoothing value of 3.981072. The chronogram indicated that the common ancestors of the K. rugosus and K. waterhousei complexes arose at approximately the same time between 3.38 and 6.05 mya. The age of the root of the phylogeny was dated at between 10.00 and 10.43 mya. Host affiliation inferred using a stochastic character mapping approach implemented in SIMMAP indicated a host switch to a Plurinerves ancestor (the probability of a Plurinerves ancestor at this node = 99.9%) at approximately 7.5 mya.
We used likelihood ratio tests to detect branch lengths that were not significantly different than zero to assess the possibility of hard molecular polytomies. The genetic data were divided into six partitions to generate Bayesian inferences. As different partitions showed branch length variation, we mapped branch lengths not significantly different from zero (at the α = 0.05 level) for 6/6 partitions, 5/6 partitions, 4/6 partitions, and no partitions onto our Bayesian consensus phylogram (Figure 1). There was a high density of internal and terminal branch lengths not significantly different from zero for 6/6, 5/6, and 4/6 partitions within the K. rugosus and K. waterhousei complexes. For the 5/6 partitions of branch lengths not significantly different from zero, the non-zero length partition was always derived from the mitochondrial DNA data (COI and 16S), which might be expected given that these gene regions have higher substitution rates than the other partitions. When the data were pooled together (i.e. no partitions), branch lengths not significantly different from zero always coincided with all or a high proportion of branch lengths not significantly different from zero indicated by the partitioned analyses.
SYMMETREE performs seven slightly different alternatives of diversification rate tests, each progressively more sensitive to different nodal depth scales . When testing for rate variation within a tree, significant tree asymmetry (Figure 1) was detected through the most to least sensitive test-statistics (single-tree 0.0001 < P < 0.0315; batch processing 0.000 < P < 0.000). Whole-tree analysis for the detection of tree asymmetry indicated that lineages within the gall-thrips phylogeny diversified at significantly different rates. Diversification rate shifts inferred from the best Bayesian consensus phylogeny are conditional on its accuracy and also implicitly assume full representation and/or random sampling of gall-thrips taxa. The phylogeny was lacking some gall-thrips taxa but increased the representation of known taxa from approximately 35%  to 90% and was assumed not to compromise the analysis.
Given significant levels of tree asymmetry, branches along which putative rate shifts occurred can be identified. The significant rate shift identified in a branch in the single-tree analysis coincides with the node of the most recent ancestor of the Kladothrips antennatus (Moulton) lineage (PΔ1 = 0.0363636 and PΔ2 = 0.0476392). Substantial but not significant rate shifts were identified at the most recent ancestral branch to the Kladothrips morrisi (Mound, Crespi, & Kranz) lineage (PΔ1 = 0.0583 and PΔ2 = 0.075) and most recent ancestral branch to the K. rodwayi lineage (PΔ1 = 0.066 and PΔ2 = 0.066). Of the 100 replicates in our batch processing analysis used to account for phylogenetic uncertainty, the significant diversification rate shift at the ancestor of K. antennatus, was consistently identified along the same branch although slight tree-topology changes occur among the replicate sample.
On the assumption that gall-thrips and Acacia co-radiated, chronological conclusions indicate the common ancestor of gall-thrips arose during the commencement of long-term and widespread desertification in Australia towards the end of the Miocene epoch. The colonisation of a novel ancestral host, related to the extant Plurinerves, occurred before the inferred onset of climatic cycling and more rapid desert expansion over the Quaternary period. This host switch appears to have afforded greater opportunity for gall-thrips diversification on Plurinerves compared to species specialising on an alternative host section. Sequence divergences amongst gall-thrips specialising on Plurinerves suggest this group has been subject to a rapid and relatively recent diversification episode and this is consistent with processes of climatic cycling during the Quaternary period.
Bayesian inference indicated two well-supported, well-resolved monophyletic clades: one comprising K. ellobus,K. acaciae, and the K. rugosus complex, and the other comprising K. hamiltoni, K. harpophyllae, and the K. waterhousei, K. habrus and K. intermedius complexes (Figure 1). These taxa are all affiliated with Plurinerves hosts; however, each of the clades includes either K. maslini or K. rodwayi, lineages that have likely undergone a host-switching event [52, 53] (Figure 2). Other data  and phylogenetic patterns  indicates these gall thrips clades have diversified in parallel via some combination of cospeciation, highly restricted host-plant switching, or both processes. The less than strict form of cospeciation between these gall-thrips clades and the host species they share implies that underlying processes impacting diversification might act in tandem for both clades on highly-related Plurinerves. This is in contrast to lineages inhabiting more distantly-related host species.
Indications of processes driving gall-thrips diversification acting on the K. rugosus and K. waterhousei complexes might be expressed differently in K. rodwayi and K. maslini as a consequence of host switching. These two species inhabit phylogenetically distant host taxa [48, 62], are mesic-zone distributed [51, 63], and share secondarily acquired plesiotypic life history strategies. The inferred host switches of the K. rodwayi and K. maslini lineages might represent a shift to enemy free space [1, 38]. Phylogenetic inference suggests Koptothrips species specialise on gall-thrips species  and that as a consequence might also diversify in parallel with the host thrips after the host switch [64, 65] and this is consistent with the presence of Koptothrips parasitising K. rodwayi galls. Very little life history information has been collected for K. maslini in contrast to that for K. rodwayi discussed below.
The inferred host switch of an ancestor of K. rodwayi apparently resulted in a loss of soldiers (sociality), the retention of small gall size, small brood sizes similar to social species, and adult eclosion in the gall before dispersal . The striking life history shift and presumed subsequent bottleneck effects resulting from the host switch would be expected to influence the level of sequence divergence observed in the K. rodwayi branch. The phylogram (Figure 1) shows a terminal branch for the K. rodwayi lineage similar to those of many other branches leading to described gall-thrips species. The relative level of differentiation, assuming the sampled genetic change reflects observed phenotypic change (Figure 1) might help explain why K. rodwayi has retained some features of closely related social species in this clade, including small brood numbers and small gall sizes (the social species include those from K. harpophyllae down to K. intermedius except for K. rodwayi and K. xiphius in Figure 1).
Life history shifts appear to be more acute subsequent to a host-switching event . Dramatic life history shifts appear to have coincided with gall-thrips association with Plurinerves hosts. For example, gall-thrips on Plurinerves have evolved behaviourally and morphologically specialised defensive castes, cofounding between male and female, and adult eclosion outside of the natal gall . The proliferation of gall-thrips lineages on Plurinerves hosts, under the assumption that the extinction rate for each group is more or less equivocal, contrasts with those on Juliflorae hosts and suggests that each group was subject to variation in processes affecting diversification that might be linked to host evolution. Host-related races present in the species complexes inhabiting Plurinerves do not occur for the K. maslini and K. rodwayi lineages on more distantly-related hosts distributed in non-arid climates. Host-plant diversity has been found to play a key role underlying the rates at which host-switching, and therefore insect diversification, can take place [66, 67]. However, the opportunity for increased diversification after the successful colonisation of a novel niche need not necessarily follow, and perhaps the processes driving diversification might differ between host groups. Taken together, these patterns suggest that diversification in gall-thrips is closely linked to host evolution in addition to host affiliation.
We estimated absolute timing of lineage diversification and mapped host affiliation onto our Bayesian consensus tree (Figure 1). In the absence of direct evidence of gall-thrips divergence times, co-radiation between thrips and Acacia has been treated as an assumption and the ratio of timing between the origin and radiation of gall-thrips specialising on Plurinerves hosts tested. Independent analysis of each calibration point supported the timing of the alternative calibration date and a chronogram was generated using both constraints. Penalised likelihood estimates and ancestral host affiliation inferences show a host switch to a Plurinerves ancestor approximately 7.5 mya, before more extreme arid-zone expansion during the Quaternary period. The common ancestors of the K. rugosus and K. waterhousei complexes both occurred at approximately the same time approximately 3.6 mya. The coincidental diversification of the two complexes, each specialising on the same set of Plurinerves host species (Figure 3), suggest that a component of diversification opportunities for gall-thrips was a consequence of host range expansion and host speciation. Floral communities on desert margins in Australia would have been sensitive to glacial extremes and climatic cycling [21–24] and speciation in the sclerophyllous taxa was partly due to population fragmentation and lability induced by these upheavals [19, 25, 26].
Diversification rate changes
Our evidence supports Hopper and Gioia's  prediction that phylogenetic pattern showing unresolved relationships towards the terminal branches of a tree attests to rapid increase in diversification/speciation. The high incidence of molecular polytomies and short branch lengths towards the terminal ends of the phylogeny generally occur for gall-thrips lineages that specialise on Plurinerves and not Juliflorae. The presence of hard molecular polytomies indicated that low support within the K. rugosus and K. waterhousei complexes (Figure 1) might be the outcome of rapid diversification in these lineages [68, 69].
Furthermore, diversification rate variation analyses identified significant tree asymmetry at a branch believed to represent the transition from gall-thrips lineages that display a plesiotypic life history (Figure 2). The four species, Kladothrips zygus (Mound, Crespi, & Kranz), Kladothrips pilbara (Mound, Crespi, & Kranz), Kladothrips schwarzi (Mound, Crespi, & Kranz), and K. antennatus are considered to be similar in biology to ancestral gall-inducing thrips. These species display the most primitive morphology and gall structure, inhabit Juliflorae hosts, and are distributed in the most arid regions of northwestern and central Australia . Juliflorae such as Acacia aneura (F. Muell ex. Benth), supports K. antennatus and three other centrally distributed species, Kladothrips arotrum (Mound), Kladothrips sterni (Mound, Crespi, & Kranz), and Kladothrips tepperi (Uzel).
The underlying causes of the diversification rate change before the origins of the K. rugosus and K. waterhousei complexes are difficult to test. The inferred rate change occurred early in the evolution of gall-thrips Acacia (Figure 1), and therefore might represent a footprint of central arid-zone expansion before the proposed Plurinerves host range expansion on the arid-zone periphery (Figure 3) resulting from climatic cycling. The central Australian desert is known to have originated as severe aridity developed during the Pliocene from approximately 7 mya [15, 16, 18, 70]. Truly arid-zone host populations might be less evolutionarily labile than populations in semi-arid regions. Phenotypic diversity and species richness appear to have been amplified on the Plurinerves lineage compared to lineages supported by centrally distributed Juliflorae (Figure 4) over the last 4 myr assuming our chronogram is a reasonable approximation. It is possible that fragmentation featured more prominently in the Plurinerves during the most recent and extensive speciation event. The Juliflorae might have been able to maintain gene flow sufficient to arrest fragmentation effects apparently experienced by the Plurinerves.
This work has addressed hypotheses connecting gall-thrips phylogeny with host-plant evolution and suggests that host expansion and climate change have important consequences for gall-thrips diversification. A pronounced diversification episode for gall-thrips lineages affiliated with Plurinerves hosts appears to have commenced between 3 and 6 mya. The inferred host-plant switch to a Plurinerves ancestor relatively early in the evolution of gall-thrips, preceded the onset of pronounced diversification for this lineage. In addition to the cospeciation and host switching evident for this group, Acacia cladogenesis would provide an expanding resource and unoccupied ecological niches to exploit. These patterns agree with the climatic cycling during the Quaternary period contriving an opportunity for some phytophagous insects, associated with a particular flora, to diversify at heightened rates with respect to others. This work suggests a component of the plant-feeding insect diversity in Australia has been passively driven by host evolution.
The phylogenetic analyses included a great majority of known morphotypes and all described species . No isolates were available for the types not included in the analyses. A comprehensive explanation of DNA extraction, PCR, and alignment protocols, and choices for substitution models is given in McLeish et al. . Up to 1,245 bp of the cytochrome oxidase one (COI) mitochondrial gene, 444 bp of the elongation factor one alpha (EF-1α) gene, 472 bp of the 16S (ribosomal RNA subunit) gene, and 549 bp of the wingless gene were amplified. Sequence data for the K. waterhousei complex at EF-1α and wingless gene regions was not available and absent for all analyses. Sequences were aligned using ClustalX 1.81.1sa software (; ftp://ftp-igbmc.u-strasbg.fr/pub/ClustalX/ accessed 24 June 2005). Insertions and deletions were removed from the sequence alignment before analyses. All nucleic acid sequence data has been lodged with GenBank under the accession numbers AY827474–AY827481, AY920988–AY921000, AY921058–AY921069, and DQ246453–DQ246516. Bayesian inferences were implemented in PAUP*b4.10  and MrBayes (MrBayes 3.0b4, ).
To accommodate differences in substitution rate parameters and heterogeneity of base composition in our multiple gene fragment dataset, we fitted separate models to different gene partitions in the Bayesian analysis [74, 75]. The sequence data used in the MrBayes analysis was divided into six partitions comprising 1st, 2nd, and 3rd codon positions of the COI mitochondrial data, with single partitions for each of EF-1α, wingless, and the 16S gene fragments. We used a general time reversible (GTR) DNA substitution model with gamma distributed rates with a proportion of invariant sites. Posterior probabilities and mean branch lengths are derived from 3,000 trees taken from generations 3.5–5.0 million, sampling every 500th generation. The sampled trees were derived from post-burnin generations after the chains had reached apparent stationarity. We ran the Bayesian analysis three times to verify the repeatability of the phylogenetic outcome using the same priors as above and obtained consistent and stable posterior values. There was a 0.16% difference in the arithmetic mean of posterior probabilities between the three MrBayes runs.
The outgroup, Rhopalothripoides Bagnall, is the most closely related sister-genus to Kladothrips Froggatt . The K. rugosus and K. waterhousei species complexes were chosen as ingroup taxa, as data strongly implies that these taxa have diversified recently and in parallel [52, 53] and constitute the majority of known gall-thrips morphotypes remaining to be sampled. The approximation for the origin of each of these complexes is therefore expected to be contemporaneous.
Construction of chronograms
The molecular clock hypothesis  of equal rates across lineages can be examined using a likelihood-ratio test. The GTR + I + G model for all gene regions in a DNA sequence data set was assumed in the analysis. The null hypothesis is that the likelihood is maximized under the constraint of equal rates across lineages (L 0), that is, an enforced molecular clock model. The alternative hypothesis relaxes the clock constraint by assigning a different rate to each of the lineages; the likelihood under the alternative hypothesis is L 1. The likelihood-ratio test statistic (twice the difference in the log e likelihood, -2 log e Λ; Λ = L 0/L 1) between the null and alternative models is approximately χ2 distributed with s – 2 degrees of freedom. The molecular clock hypothesis was rejected at the α = 0.05 level (log e L 0 = -16,925.01, log e L 1 = -16,811.27, -2 log e Λ = 227.48, P < 0.0001).
To better accommodate unequal substitution rates among lineages, a semi-parametric penalised likelihood (PL) rate smoothing with a truncated Newton algorithm was implemented to estimate relative ages of nodes using r8s . Penalised likelihood imposes constraints on rate variation combining a model allowing substitution rate variation among branches with a roughness penalty that mediates rate changes from branch to branch. A "smoothing parameter" that determines the relative contribution of each was determined by a cross-validation procedure. To generate a PL chronogram, we used MrBayes to generate a consensus phylogeny using the same priors as described above. Modeltest 3.0  was used to determine the appropriate nucleotide substitution model (GTR + I + G) and gamma shape required for r8s analysis. The outgroup Rhopalthripoides was pruned prior to analyses using r8s. The 95% central distribution intervals for node-age estimates were generated by filtering post-burnin trees, retaining only those that had an identical topology to the consensus tree produced by our Bayesian analysis. This resulted in 258 sampled trees from the MCMC run. We used r8s to generate chronograms for each of these filtered trees. The resulting branch length estimates were used to calculate the ages of key nodes for each tree and the 2.5% right and left tails were then removed, leading to 95% central distribution limits that could be used as a measure of confidence for the point estimates .
In the absence of even a modest amount of evidence bearing directly on gall-thrips divergence times, fossil Acacia pollen and biogeographical evidence was used to calibrate the phylogeny. Therefore, chronological conclusions are only valid under the assumption that gall-thrips radiations must have coincided with host radiations. This is a reasonable proposal given that gall-thrips specialising on Plurinerves display a less-than-strict form of cospeciation between insect and host [52, 53] and are therefore tightly affiliated with this resource. In addition, we aimed to show that the 'ratio' of the ages of two calibration points in the gall-thrips phylogeny is similar regardless of the absolute timing of these events.
Divergence events were calibrated using combinations of fixed and/or constrained maximum and minimum times at the root node and an internal node based on fossil Acacia pollen records and biogeographical evidence. Each of the calibration points used was individually assessed in separate analyses to compare the appropriateness of the other calibration point, and thereby determining the ratio (or fit) of calibration points. That is, the root node or an internal node was calibrated independently as a fixed age or constrained between two ages. For these tests, the root node was fixed at 25 mya and 15 mya and the internal node at 5 mya. Additionally, the root was constrained between 10–15 myr or the internal node between 2–5 myr. Constrained calibration points were presumed to be a more realistic prior because the biogeographic and fossil evidence are given in approximate terms.
Calibration points were nominated in accordance with the following evidence. The appearance of fossil Acacia pollen the fossil record in Australia 25 mya  and the distribution of the Acacia subgenera Phyllodineae hosts to which gall thrips are confined, indicate that radiations of this group occurred in Australia no more than 15 mya [24, 40]. Therefore, the appearance of gall-thrips hosts presumably coincided with the appearance of an ancestor of these arid-adapted Acacia during the Miocene; therefore we constrained the root node between 10–15 mya. A rapid increase in fossil Acacia pollen co-occurs with a period of pronounced arid-zone expansion in Australia over the last 3 myr [30, 79]. Evidence indicates speciation in host Acacia section Plurinerves occurred over a relatively short period as widespread aridity developed in the early Quaternary over the last 2–5 myr [15, 16, 53, 80, 81]. The common ancestor of the K. rugosus species complex specialising on extant Plurinerves species was therefore constrained to between 2–5 mya. The K. waterhousei species complex specialises on the same group of host species as K. rugosus and was therefore expected to have radiated contemporaneously.
To provide a convenient visualisation of historical host utilisation by gall-thrips, as gall-thrips are highly host specific , and to indicate the relative age of the transition from Juliflorae to the novel ancestor of host section Plurinerves, ancestral states were also inferred using a stochastic character mapping approach (SIMMAP: ). SIMMAP is a post-tree analysis program for making inferences about character evolution and implements a Bayesian method for mapping characters using stochastic substitution models . To avoid potentially spurious inferences based on a single topology, character histories were sampled from the posterior distribution of 1,000 trees obtained from our MrBayes analyses. We used a data matrix of host utilization to calculate the probability of a host switch to a Plurinerves ancestor. Gall-thrips host species assignments for the data matrix were taken from Crespi et al. . Plurinerves, Juliflorae, or Phyllodinae host affiliation was coded from 0 to 2 respectively.
To investigate diversification rate changes we used a whole-tree asymmetry approach implemented in SYMMETREE . The approach is appropriate for use with trees including taxa below species-level and it is robust to polytomies. Divergences were expected to be marginal as mtDNA COI uncorrected "p" distances were below that between other gall-thrips species in some instances [38, 52]. Detection of among-lineage diversification rate variation compares observed differences in sister-group diversity to a null distribution of sister-group diversity. Diversification rate variation within the phylogeny is reported by a range of test statistics that vary in their sensitivity to nodal depth scales .
Penalised likelihood estimates of root node and internal node using our Bayesian consensus phylogram.
Calibration Points (myr)
To test the hypothesis of diversification rate change further, we also used a method for detecting multifurcations, or molecular polytomies, using likelihood ratio tests [68, 69], expecting a reasonable coincidence of branch lengths not significantly different from zero with regions of diversification rate change. Low-level node support of internal branches in phylogenies can be a result of insufficient data or an artifact of short periods of rapid speciation, during which little or no informative signal is trapped. We suspected a series of poorly supported branches corresponded to rapid diversification as inclusion of additional markers did not improve node support at these nodes in several instances. We used Modeltest  to estimate the substitution rate characteristics of each partition used in our phylogenetic analysis and used likelihood ratio tests implemented in PAUP* to detect branch lengths not significantly different from zero for separate and all partitions combined.
A lineage by time diversification plot was used to show differential rates of gall-thrips lineage diversification that specialise on either Plurinerves or Juliflorae. Lineage frequencies per time were counted using the Penalised Likelihood chronogram generated by Sanderson's r8s program . The plot includes taxa most likely below that of species status where a large majority of known yet undescribed gall morphotypes  have been included. We assume equivocal extinction rates between the two groups and presume that several known morphotypes specialise on Juliflorae host species, that were not sampled, are only marginally underrepresented (see above).
We thank Laurence Mound for useful commentary from the inception of this project and the Evolutionary Biology Unit, South Australia Museum, for their sequencing facilities and technical expertise. An Australian Research Council grant awarded to Schwarz, Cooper, Crespi and Chapman (DP0346322) supported this project.
- Ehrlich PR, Raven PH: Butterflies and plants: a study in coevolution. Evolution. 1964, 18: 586-608. 10.2307/2406212.View ArticleGoogle Scholar
- Mitter C, Farrel B, Wiegmann B: The phylogenetic study of adaptive zones: has phytophagy promoted insect diversification?. Am Nat. 1988, 138: 107-128. 10.1086/284840.View ArticleGoogle Scholar
- Futuyma DJ, Moreno G: The evolution of ecological specialisation. Annu Rev Ecol Syst. 1988, 19: 207-233. 10.1146/annurev.es.19.110188.001231.View ArticleGoogle Scholar
- Jermy T: Evolution of insect-plant relationships – a devil's advocate approach. Entomol Exp Appl. 1993, 66: 3-12. 10.1007/BF02381541.View ArticleGoogle Scholar
- Farrell BD, Mitter C: Phylogeny of host affiliation: have Phyllobrotica (Coleoptera: Chrysomelidae) and the Lamiales diversified in parallel?. Evolution. 1998, 44: 1389-1403. 10.2307/2409325.View ArticleGoogle Scholar
- Ronquist F: Phylogenetic approaches in coevolution and biogeography. Zool Scr. 1998, 26: 313-322. 10.1111/j.1463-6409.1997.tb00421.x.View ArticleGoogle Scholar
- Kelley ST, Farrell BD, Mitton JB: Effects of specialisation on genetic differentiation in sister species of bark beetles. Heredity. 2000, 84: 218-227. 10.1046/j.1365-2540.2000.00662.x.View ArticlePubMedGoogle Scholar
- Craig TP, Horner JD, Itami JK: Genetics, experience, and host-plant preference in Eurosta solidaginis : implications for host shifts and speciation. Evolution. 2001, 55: 773-782. 10.1554/0014-3820(2001)055[0773:GEAHPP]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Nyman T: The willow bud galler Euura mucronata Hartig (Hymenoptera: Tenthredinidae): one polyphage or many monophages?. Heredity. 2002, 28: 288-295. 10.1038/sj.hdy.6800042.View ArticleGoogle Scholar
- Johnson KP, Clayton DH: Coevolutionary history of ecological replicates: comparing phylogenies of wing and body lice to Columbiform hosts. Tangled Trees. Edited by: Page RDM. 2003, Chicago, IL: University of Chicago Press, 262-286.Google Scholar
- Page RDM: Tangled Trees. 2000, Chicago, IL: University of Chicago PressGoogle Scholar
- Ward LK, Hackshaw A, Clarke RT: Do food-plant preferences of modern families of phytophagous insects and mites reflect past evolution with plants. Biol J Linnean Soc. 2003, 78: 51-83. 10.1046/j.1095-8312.2003.00128.x.View ArticleGoogle Scholar
- Janz N, Nylin S, Wahlberg N: Diversity begets diversity: host expansions and the diversification of plant-feeding insects. BMC Evol Biol. 2006, 6: 4-10.1186/1471-2148-6-4.PubMed CentralView ArticlePubMedGoogle Scholar
- Cranston PS, Naumann ID: Biogeography. The Insects of Australia. Edited by: Naumann ID. 1991, Melbourne, Victoria: Division of Entomology, CSIRO Australia/Melbourne University PressGoogle Scholar
- Gaston KJ: Species-range size distributions: products of speciation, extinction and transformation. Philos Trans R Soc London Biol. 1998, 353: 219-230. 10.1098/rstb.1998.0204.View ArticleGoogle Scholar
- Maslin BR, Hopper SD: Phytogeography of Acacia (Leguminosae: Mimosoideae) in central Australia. Evolution of the Flora and Fauna of Arid Australia. Edited by: Barker WR, Greenslade PJM. 1982, Hong Kong, Peacock PublicationsGoogle Scholar
- Clapperton CM: Quaternary glaciations in the Southern Hemisphere: an overview. Quat Sci Rev. 1990, 9: 299-304. 10.1016/0277-3791(90)90024-5.View ArticleGoogle Scholar
- Niklas KJ: A mechanical perspective on foliage leaf form and function. New Phytol. 1999, 143: 19-31. 10.1046/j.1469-8137.1999.00441.x.View ArticleGoogle Scholar
- Markgraf V, McGlone M, Hope G: Neogene paleoenvironmental and paleoclimatic change in southern temperate ecosystems – a southern perspective. Trends Ecol Evolut. 1995, 10: 143-147. 10.1016/S0169-5347(00)89023-0.View ArticleGoogle Scholar
- Lovejoy TE, Hannah L: Climate Change and Biodiversity. 2004, New Haven, CT: Yale University PressGoogle Scholar
- Crisp MD, Laffan S, Linder HP, Monro A: Endemism in the Australian flora. J Biogeogr. 2001, 28: 183-198. 10.1046/j.1365-2699.2001.00524.x.View ArticleGoogle Scholar
- Burgman MA: Are listed threatened plant species actually at risk?. Aust J Bot. 2002, 50: 1-13. 10.1071/BT01052.View ArticleGoogle Scholar
- Chapple DG, Keogh JS: Parallel adaptive radiations in arid and temperate Australia: molecular phylogeography and systematics of the Egernia whitii (Lacertilia: Scincidae) species group. Biol J Linnean Soc. 2004, 83: 157-173. 10.1111/j.1095-8312.2004.00378.x.View ArticleGoogle Scholar
- Hopper SD: Biogeographical aspects of the speciation in the southwest Australian flora. Annu Rev Ecol Syst. 1979, 10: 339-422. 10.1146/annurev.es.10.110179.002151.View ArticleGoogle Scholar
- Truswell EM, Harris WK: The Cainozoic palaeobotanical record in arid Australia: fossil evidence for the origins of an arid-adapted flora. Evolution of the Flora and Fauna of Arid Australia. Edited by: Barker WR, Greenslade PJM. 1982, Hong Kong: Peacock publicationsGoogle Scholar
- Anderson S: Area and endemism. Q Rev Biol. 1994, 69: 451-471. 10.1086/418743.View ArticleGoogle Scholar
- Crespi BJ, Carmean DA, Mound LA, Worobey M, Morris DC: Phylogenetics of social behaviour in Australian gall-forming thrips: evidence from mitochondrial DNA sequence, adult morphology and behaviour, and gall morphology. Mol Phylogenet Evol. 1998, 9: 163-180. 10.1006/mpev.1997.0449.View ArticlePubMedGoogle Scholar
- Crespi BJ, Worobey M: Comparative analysis of gall morphology in Australian gall thrips: the evolution of extended phenotypes. Evolution. 1998, 52: 1686-1696. 10.2307/2411341.View ArticleGoogle Scholar
- Price PW, Fernandes GW, Lara ACF, Brown J, Barrios H, Wright MG, Ribeiro SP, Rothcliff N: Global patterns in local number of insect galling species. J Biogeogr. 1998, 25: 581-591. 10.1046/j.1365-2699.1998.2530581.x.View ArticleGoogle Scholar
- Morris DC, Schwarz MP, Cooper SJB, Mound LA: Phylogenetics of Australian Acacia thrips: the evolution of behaviour and ecology. Mol Phylogenet Evol. 2002, 25: 278-292. 10.1016/S1055-7903(02)00258-0.View ArticlePubMedGoogle Scholar
- Crisp M, Cook L, Steane D: Radiation of the Australian flora: what can comparisons of molecular phylogenies across multiple taxa tell us about the evolution of diversity in present-day plant communitie?. Philos Trans R Soc London Biol. 2004, 359: 1551-1571. 10.1098/rstb.2004.1528.View ArticleGoogle Scholar
- Hopper SD, Gioia P: The southwest Australian floristic region: evolution and conservation of a global hot-spot of biodiversity. Annu Rev Ecol Syst. 2004, 35: 623-650. 10.1146/annurev.ecolsys.35.112202.130201.View ArticleGoogle Scholar
- Fernandes GW, Price PW: Biogeographical gradients in galling species richness. Tests of hypotheses. Oecol. 1988, 76: 161-167. 10.1007/BF00379948.View ArticleGoogle Scholar
- Fernandes GW, Lara ACF, Price PW: The geography of galling insects and the mechanisms that result in patterns. The Ecology and Evolution of Gall-Forming Insects. Edited by: Price PW, Mattson WJ. 1994, Minnesota: United States Department of Agriculture, 42-48.Google Scholar
- Blanche KR, Westoby M: Gall-forming insect diversity is linked to soil fertility via host plant taxon. Ecology. 1995, 76: 2334-2339. 10.2307/1941706.View ArticleGoogle Scholar
- Veldtman R, McGeoch MA: Gall-forming insect species richness along a non-scleromorphic vegetation rainfall gradient in South Africa: the importance of plant community composition. Aust Ecol. 2003, 28: 1-13. 10.1046/j.1442-9993.2003.01234.x.View ArticleGoogle Scholar
- Ananthakrishnan TN: Unique aspects in the biology of thrips-induced galls. Biology of Insect Induced Galls. Edited by: Shorthouse JD, Rohfritsch O. 1992, Oxford: Oxford University Press, 185-195.Google Scholar
- Morris DC, Mound LA, Schwarz MP, Crespi BJ: Morphological phylogenetics of Australian gall-inducing thrips and their allies: the evolution of host plant affiliations, domicile use, and social behaviour. Syst Entomol. 1999, 24: 1-11. 10.1046/j.1365-3113.1999.00083.x.View ArticleGoogle Scholar
- Boughton VH: Phyllode structure, taxonomy and distribution in some Australian Acacias. Aust J Bot. 1986, 34: 663-674. 10.1071/BT9860663.View ArticleGoogle Scholar
- Maslin BR, Miller JT, Seigler DS: Overview of the generic status of Acacia (Leguminosae: Mimosoideae). Aust Syst Bot. 2003, 16: 1-18. 10.1071/SB02008.View ArticleGoogle Scholar
- Mound LA: Thrips and gall induction: a search for patterns. Plant Galls. Edited by: Williams AJM. 1994, Oxford: Clarendon Press, 131-149.Google Scholar
- Atkin OK, Schortemeyer N, McFarlane N, Evans JR: Variation in the components of relative growth rates in ten Acacia species from contrasting environments. Plant Cell Environ. 1998, 21: 1007-1017. 10.1046/j.1365-3040.1998.00356.x.View ArticleGoogle Scholar
- Niklas KJ: A mechanical perspective on foliage leaf form and function. New Phytol. 1999, 143: 19-31. 10.1046/j.1469-8137.1999.00441.x.View ArticleGoogle Scholar
- Doley D: Evolution of plant water relations' research in Australia. Functional Plant Biol. 2004, 31: 405-414. 10.1071/FP03210.View ArticleGoogle Scholar
- Nelson EC: Disjunct plant distributions on the south-western Nullabor Plain, Western Australia. J Roy Soc West Aust. 1974, 57: 105-107.Google Scholar
- Parker AJ, Flemming CM, Flint RB: Geology. Natural History of the Eyre Peninsula. Edited by: Twidale CR, Tyler MJ, Davies M. 1985, Adelaide: Royal Society of South Australia, 21-45.Google Scholar
- Haddad D, Watts AB, Lindsay J: Evolution of the intracratonic Officer Basin, central Australia: implications from subsistence analysis and gravity modelling. Basin Res. 2001, 13: 217-238. 10.1046/j.1365-2117.2001.00147.x.View ArticleGoogle Scholar
- Maslin BR: Acacia. Flora of Australia Mimosaceae, Acacia part 1. Edited by: Orchard AE, Wilson AJG. 2001, Melbourne: ABRS/CSIRO Publishing, 11A:Google Scholar
- Cracraft J: Explaining patterns of biological diversity: integrating causation at different spatial and temporal scales. Systematics, Ecology and the Biodiversity Crisis. Edited by: Eldridge N. 1992, New York: Columbia University Press, 59-76.Google Scholar
- McKinney ML, Drake JA: Biodiversity Dynamics. 1998, New York: Columbia University PressGoogle Scholar
- Morris DC, Schwarz MP, Crespi BJ, Cooper JB: Phylogenetics of gall-inducing thrips on Australian Acacia. Biol J Linnean Soc. 2001, 74: 73-86. 10.1006/bijl.2001.0575.View ArticleGoogle Scholar
- McLeish MJ, Crespi BJ, Chapman TW, Schwarz MP: Parallel diversification of Australian gall-thrips on Acacia. In review, Mol Phylogenet Evol.Google Scholar
- Crespi BJ, Morris DC, Mound LA: Evolution of Ecological and Behavioural Diversity: Australian Acacia Thrips as Model Organisms. 2004, Canberra: Australian Biological Resources Study & Australian National Insect Collection, CSIROGoogle Scholar
- Miller JT, Andrew R, Bayer RJ: Molecular phylogenetics of the Australian acacias of subg. Phyllodineae (Fabaceae: Mimosoideae) based on the trnK intron. Aust J Bot. 2003, 51: 167-177. 10.1071/BT01099.View ArticleGoogle Scholar
- Murphy DJ, Miller JT, Bayer RJ, Ladiges PY: Molecular phylogeny of Acacia subgenus Phyllodineae (Mimosoideae: Leguminosae) based DNA sequences of the internal transcribed spacer region. Aust Syst Bot. 2003, 16: 19-26. 10.1071/SB01042.View ArticleGoogle Scholar
- Christidis L, Schodde R: Relationships and radiations in the Meliphagine Honeyeaters, Meliphaga, Lichenostromus and Xanthotis (Aves: Meliphagidae): Protein evidence and its integration with morphology and ecogeography. Aust J Zool. 1993, 41: 293-316. 10.1071/ZO9930293.View ArticleGoogle Scholar
- Strasburg JL, Kearney M: Phylogeography of sexual Heteronotia binoei (Gekkonidae) in the Australoian arid zone: climatic cycling and repetitive hybridisation. Mol Ecol. 2005, 14: 2755-2772. 10.1111/j.1365-294X.2005.02627.x.View ArticlePubMedGoogle Scholar
- Vassal J: Apport des recherches ontogenique et seminologique a l'etude morphologique, taxonomique et phylogenique du genre Acacia. Bull Soc Hist Nat Toulouse. 1972, 108: 125-247.Google Scholar
- McLeish MJ, Chapman TW, Mound LA: Gall morpho-type corresponds to separate species of gall-inducing thrips (Thysanoptera: Phlaeothripidae). Biol J Linnean Soc. 2006, 88: 555-56360. 10.1111/j.1095-8312.2006.00641.x.View ArticleGoogle Scholar
- Posada D, Crandall KA: MODELTEST: Testing the model of DNA substitution. Bioinformatics. 1998, 14: 817-818. 10.1093/bioinformatics/14.9.817.View ArticlePubMedGoogle Scholar
- Chan KMA, Moore BR: SymmeTREE: whole-tree analysis of differential diversification rates. Bioinfomatics. 2005, 21: 1709-1710. 10.1093/bioinformatics/bti175.View ArticleGoogle Scholar
- Pedley L: Acacias in Queensland. 1987, Queensland: Department of Primary Industry, Queensland GovernmentGoogle Scholar
- Kranz B, Schwarz MP, Morris DC, Crespi BJ: Life history of Kladothrips ellobus and Oncothrips rodwayi: insight into the origin and loss of soldiers in gall-inducing thrips. Ecol Entomol. 2002, 27: 49-57. 10.1046/j.1365-2311.2002.0380a.x.View ArticleGoogle Scholar
- Brown JM, Abrahamson WG, Packer RA, Way PA: The role of natural enemy escape in a gallmaker host-plant shift. Oecol. 1995, 104: 52-60. 10.1007/BF00365562.View ArticleGoogle Scholar
- Eubanks MD, Blair CP, Abrahamson WG: One host shift leads to another? Evidence of host-race formation in a predaceous gall-boring beetle. Evolution. 2003, 57: 168-172. 10.1554/0014-3820(2003)057[0168:OHSLTA]2.0.CO;2.View ArticlePubMedGoogle Scholar
- Craig TP, Itami JK, Horner JD, Abrahamson WG: Host shifts and speciation in gall-forming insects. The Ecology and Evolution of Gall-Forming Insects. Edited by: Price PW, Mattson WJ, Baranchikov YN. 1994, General Technical Report NC-174. St. Paul, MN: United States Department of Agriculture, 194-207.Google Scholar
- Mendonca MS: Galling insect diversity patterns: the resource synchronisation hypothesis. Oikos. 2001, 95: 171-176. 10.1034/j.1600-0706.2001.950120.x.View ArticleGoogle Scholar
- Goldman N, Whelan S: Statistical tests of Gamma-distributed rate heterogeneity in models of sequence evolution in phylogenetics. Mol Biol Evol. 2000, 17: 975-978.View ArticlePubMedGoogle Scholar
- Slowinski JB: Molecular polytomies. Mol Phylogenet Evol. 2001, 19: 114-120. 10.1006/mpev.2000.0897.View ArticlePubMedGoogle Scholar
- Wasson RJ: Landform development in Australia. Evolution of the Flora and Fauna of Arid Australia. Edited by: Barker WR, Greenslade PJM. 1982, Frewville, South Australia: Peacock Publications, 23-33.Google Scholar
- Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997, 24: 4876-4882. 10.1093/nar/25.24.4876.View ArticleGoogle Scholar
- Swofford DL: PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4.0b10. 2002, Sunderland, MA: SinauerGoogle Scholar
- Huelsenbeck JP, Ronquist F: MRBAYES: Bayesian inference of phylogeny. Bioinformatics. 2001, 17: 754-755. 10.1093/bioinformatics/17.8.754.View ArticlePubMedGoogle Scholar
- Lin CP, Danforth BN: How do insect nuclear and mitochondrial gene substitution patterns differ? Insights from Bayesian analyses of combined data sets. Mol Phylogenet Evol. 2004, 30: 686-702. 10.1016/S1055-7903(03)00241-0.View ArticlePubMedGoogle Scholar
- Brandley MC, Schmitz A, Reeder TW: Partitioned Bayesian analyses, partition choice, and the phylogenetic relationships of Scincid lizards. Syst Biol. 2005, 54: 373-390. 10.1080/10635150590946808.View ArticlePubMedGoogle Scholar
- Felsenstein J: Confidence limits on phylogenies: an approach to using the bootstrap. Evolution. 1985, 39: 783-791. 10.2307/2408678.View ArticleGoogle Scholar
- Sanderson MJ: R8s: inferring absolute rates of evolution and divergence times in the absence of a molecular clock. Bioinformatics (Oxford). 2003, 19: 301-301. 10.1093/bioinformatics/19.2.301.View ArticleGoogle Scholar
- Schwarz MP, Fuller S, Tierney SM, Cooper SJB: Molecular phylogenetics of the exoneurine allodapine bees reveal an ancient and puzzling dispersal from Africa to Australia. Syst Biol. 2006, 55: 31-45. 10.1080/10635150500431148.View ArticlePubMedGoogle Scholar
- Martin HA: Australian Tertiary phytogeography: evidence from palynology. History of the Australian Vegetation: Cretaceous to Recent. Edited by: Hill RS. 1994, Cambridge: Cambridge University Press, 104-142.Google Scholar
- Byrne M, Macdonald B, Coates D: Phylogeographical patterns in chloroplast DNA variation within the Acacia acuminata (Leguminosae: Mimosoideae) complex in western Australia. J Evol Biol. 2002, 15: 576-587. 10.1046/j.1420-9101.2002.00429.x.View ArticleGoogle Scholar
- Andrew RL, Miller JT, Peakall R, Crisp MD, Bayer RJ: Genetic, cytogenetic and morphological patterns in a mixed mulga population: evidence for apomixis. Aust Syst Bot. 2003, 16: 69-80. 10.1071/SB01043.View ArticleGoogle Scholar
- Bollback JP: SIMMAP: Stochastic Character Mapping of Discrete Traits on Phylogenies. Version 1.0 Beta 2.0. 2004, [http://brahms.ucsd.edu/simmap.html]Google Scholar
- Nielsen R: Mapping mutations on phylogenies. Syst Biol. 2002, 51: 729-732. 10.1080/10635150290102393.View ArticlePubMedGoogle Scholar
- Moore BR, Chan KMA, Donoghue MJ: Detecting diversification rate variation in supertrees. Phylogenetic Supertrees: Combining Information to Reveal the Trees of Life. Edited by: Bininda-Emonds ORP. 2004, The Netherlands: Kluwer Academic Publishers, 1-47.Google Scholar
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