The DNA binding parvulin Par17 is targeted to the mitochondrial matrix by a recently evolved prepeptide uniquely present in Hominidae
- Daniel Kessler†1,
- Panagiotis Papatheodorou†2,
- Tina Stratmann1,
- Elke Andrea Dian2,
- Cristina Hartmann-Fatu1,
- Joachim Rassow2,
- Peter Bayer1Email author and
- Jonathan Wolf Mueller1Email author
© Kessler et al; licensee BioMed Central Ltd. 2007
Received: 16 February 2007
Accepted: 17 September 2007
Published: 17 September 2007
The parvulin-type peptidyl prolyl cis/trans isomerase Par14 is highly conserved in all metazoans. The recently identified parvulin Par17 contains an additional N-terminal domain whose occurrence and function was the focus of the present study.
Based on the observation that the human genome encodes Par17, but bovine and rodent genomes do not, Par17 exon sequences from 10 different primate species were cloned and sequenced. Par17 is encoded in the genomes of Hominidae species including humans, but is absent from other mammalian species. In contrast to Par14, endogenous Par17 was found in mitochondrial and membrane fractions of human cell lysates. Fluorescence of EGFP fusions of Par17, but not Par14, co-localized with mitochondrial staining. Par14 and Par17 associated with isolated human, rat and yeast mitochondria at low salt concentrations, but only the Par17 mitochondrial association was resistant to higher salt concentrations. Par17 was imported into mitochondria in a time and membrane potential-dependent manner, where it reached the mitochondrial matrix. Moreover, Par17 was shown to bind to double-stranded DNA under physiological salt conditions.
Taken together, the DNA binding parvulin Par17 is targeted to the mitochondrial matrix by the most recently evolved mitochondrial prepeptide known to date, thus adding a novel protein constituent to the mitochondrial proteome of Hominidae.
Peptidyl prolyl cis/trans isomerases (PPIases, EC 126.96.36.199) play a role in protein folding and function by twisting the backbone of target proteins. PPIases are abundantly expressed in virtually all organisms and all cellular compartments [1–3]. One subclass of PPIases are parvulins, a group of enzymes involved in mitotic regulatory mechanisms and cell proliferation [4–8]. The human genome contains two parvulin genes. One encodes the well studied mitotic regulator Pin1, which isomerizes phosphorylated Ser/Thr-Pro motifs  and thereby is involved in a variety of cellular processes and conditions such as cell cycle regulation, cancer and Alzheimer's disease ; the other, the Par14/PIN4 locus on chromosome Xq13.1, gives rise to two protein species, Par14 and Par17 . Par14 (also known as PIN4/EPVH/hPar14) [11, 12] is assumed to be involved in cell cycle progression or chromatin remodeling [13–15]. Par14-like protein sequences are found in all multicellular organisms but are absent from yeasts . Although Par14 cannot rescue the lethal phenotype of Ess1 deletion, which is the only parvulin-type PPIase in yeast , Uchida and co-workers proposed a compensating function for Par14 upon Pin1 inhibition or deletion in mammalian cells . Par14 displays only very weak PPIase activity towards peptidic substrates  and hence might also perform cellular regulatory functions other than Pin1. The Par14 protein was initially detected within the nucleus, cytosol and mitochondria of human HEK 293 cells , but how this distribution was accomplished remained to be determined. Its distribution between the nucleus and cytosol [13, 15, 17] was described to be regulated in a phosphorylation-dependent manner  and deletion studies pointed to a nuclear import signal within the positively charged N-terminal section . Within the nucleus, Par14 was reported to bind to pre-ribosomal ribonucleoprotein particles , and sequence-specifically to bent double-stranded DNA . The basic N-terminal part was indispensable for high affinity DNA binding . Such bent AT-rich segments of DNA are supposed to dictate nucleosome positioning  and play a role in transcription initiation.
Par17 is a recently described protein species  also encoded by the Par14/PIN4 locus on chromosome Xq13.1. Its elongated mRNA originates from alternative transcription initiation and occurs in all human tissues tested so far . The 5'extension includes a 75 bp extended open reading frame whose expression was confirmed by in vitro translation reaction and by Western blotting of human HeLa and HepG2 cell lysates . Due to its molecular mass of 16.6 kDa, the novel parvulin was denoted as Par17 following the E. coli Par10 and human Par14 nomenclature. The longer parvulin protein is encoded within the human genome but is absent from rodent, bovine and non-mammalian genomes .
The present study shows that Par17 occurs only in genomes from great apes species (Hominidae), but not in those of other primates, as shown by parvulin sequences from different primate genomic DNA samples. Par17 is directed to mitochondria by the novel N-terminal domain that functions as non-cleavable mitochondrial targeting peptide. The protein associates with mitochondrial surfaces and is transported into the matrix in a time and membrane potential-dependent manner. Par17 binds to double-stranded DNA at physiological salt concentrations as Par14 does. Par17 is thus a Hominid-specific DNA-binding constituent of the mitochondrial matrix that has not been detected in any recent mitochondrial proteomic studies. In addition, the prepeptide represents the most recently evolved functional mitochondrial targeting peptide known to date.
Par17 occurrence in anthropoid genomic sequences
Within the human genome, the parvulin/PIN4 locus on chromosome Xq13.1 encodes an elongated open reading frame leading to the expression of Par17 [GenBank:NM_006223]. This elongation was already reported to be absent from bovine and rodent genomes . The chimpanzee genome sequence  contained a gap at the corresponding locus preventing a comparison to other genomic parvulin sequences. Surprisingly, the macaque genome that became publicly available at that time did not contain an extended Par17. Therefore, further analysis of the occurrence of an elongated parvulin within the genomes of anthropoid species became of interest as Par17's extension must have been acquired during anthropoid evolution.
As the presequence was not encoded by an additional exon, but a 5' extension of exon 1, phylogenetic studies could be performed on genomic DNA eliminating the need for fresh primate material for mRNA isolation. Genomic DNA from 10 different anthropoid species covering New World monkeys (owl monkey and cotton-top tamarin), Old World monkeys (baboon, macaque and African green monkey) and apes (gibbon, orang-utan, gorilla, chimpanzee and human) were obtained from ECACC. These genomic DNA samples originated from stable anthropoid cell lines. Primers were designed to fit to primate sequences upon comparison of the human genomic sequence with the macaque genome. A fragment of the elongated exon 1 starting directly upstream of the Par17 ATG and including part of the Par14 coding sequence was PCR amplified from all these primate sequences. Exon 3 is conserved in all mammals including mouse, with only two amino acid exchanges between humans and rodents, and was used as positive control. Hence, genomic mouse DNA could serve as an ideal control for the existence of a parvulin gene (exon 3) and the absence of an elongation (exon 1). Uniform PCR products of the expected size were obtained in all reactions with genomic DNA templates, except for mouse exon 1; these products were all gel purified, cloned and sequenced. The human and macaque sequences for exon 1 as well as human, chimpanzee, macaque and mouse sequences for exon 3 were identical to the respective Ensembl genomic sequences.
The N-terminal extension of Par17 includes an amphipatic α-helix
Predicted cellular localization of Par14 and Par17 proteins
Par17 is targeted to mitochondria within human cells
To discriminate between Par17 and Par14 localization, overexpression studies using EGFP fusions were performed. EGFP was C-terminally fused to Par17-QR and -RS proteins and to Par14 as negative control. HeLa cells grown on cover slips were transfected with the above-mentioned constructs and stained with MitoTracker, a dye that accumulates in mitochondria with intact membrane potential. Fluorescence microscopic images of these constructs in HeLa cells are shown in Figure 3B; expression as full-length proteins was verified by Western blot using an anti-GFP antibody (Figure 3C). Par17-RS- and -QR-EGFP co-localized with mitochondrial staining. Totals of 77 ± 5 and 63 ± 9% of EGFP fluorescence were measured to overlap with the MitoTracker signal for Par17-RS and -QR, respectively. Par14-EGFP fluorescence is equally distributed throughout the cytosol and partially enriched in the nucleus, as has been described previously [15, 17]. Co-localization values were far lower for Par14-EGFP (11 ± 6%) and EGFP alone (4 ± 1%).
Therefore, the presequence seemed to be the determinant for mitochondrial targeting, and the question arose whether the Par17 N-terminus was necessary and/or sufficient for mitochondrial targeting. To investigate this, EGFP was fused either to the presequence or to presequence coupled to the basic domain of Par17. Then, HeLa cells were transfected with these constructs. Their EGFP fluorescence overlapped with the MitoTracker signal to a lesser extent than the full length Par17 constructs with co-localization ratios of 49 ± 8% for presequence-RS-basic domain, 42 ± 11% for presequence-QR-basic domain, 29 ± 15% for presequence-RS and 34 ± 13% for presequence-QR. As can be deduced from these data, the mitochondrial association of Par17 is dependent on the presequence, but the basic and PPIase domains enhance mitochondrial targeting. The transfected constructs and co-localization values are schematically depicted in Figure 3D. Taken together, the cell fractionation studies and the localization of parvulin EGFP fusions provide independent evidence that Par17 isoforms are targeted to mitochondria.
Par17 import into isolated mitochondria
Next, mitochondria isolated from human, rat and yeast cells were used for proteinase K (PK) import studies, where all protein associated with mitochondria is re-isolated by centrifugation. Any protein that is not protected by translocation into sub-mitochondrial compartments will subsequently be degraded by PK. Although both Par14 and Par17-QR were degraded at 50 μg/ml PK (Figure 4B, left panel), at least 100 μg/ml PK were used throughout the following import studies. Par17 was re-isolated after incubation together with all types of mitochondria used (Figure 4B, right panel), again pointing to an association of the protein with mitochondrial organelles. Moreover, PK treatment showed that this labeled protein reached a protease-protected compartment, indicating import. Par14 also associated with mitochondria, but was not imported as it was completely degraded by PK.
Kinetic import experiments were then performed with different incubation times. Figure 4C shows the Par17 import into yeast and human mitochondria increased with time. As absolute import efficiencies cannot be compared between mitochondria from different sources, we used the fusion protein Su9-DHFR as positive control. Import into yeast mitochondria was significant, but rather low when compared to Su9-DHFR. However, Par17-QR and Su9-DHFR showed similar import efficiencies upon incubation with human mitochondria from the same preparation.
To test for the dependence on the mitochondrial membrane potential, yeast mitochondria were incubated with valinomycin either before or after the import reaction to dissipate the mitochondrial membrane potential. If import depends on the membrane potential, mitochondria with the de-coupling agent added before import would not import Par17 to the matrix. If this is not the case, Par17 should be imported irrespective of when valinomycin was added. As seen in Figure 4D, Par17 is only imported into mitochondria with intact membrane potential (lane 2 vs 3: addition of valinomycin after and before import, respectively). Therefore, the import of Par17 is dependent on an intact potential across the inner mitochondrial membrane.
To define the sub-mitochondrial localization of Par17 more precisely, a final set of import experiments were performed with reference proteins from different mitochondrial compartments. Par17 and the yeast porin, a component of the outer mitochondrial membrane were imported into rat mitochondria (Figure 4E). In a parallel reaction mitochondria were subjected to hypo-osmotic swelling, resulting in rupture of the outer membrane and loss of the porin protein. Par17, however, remained protected from PK degradation – indicating that it is at the very least transported to the inner mitochondrial membrane. It can only be degraded by PK when the mitochondria are lysed completely by the addition of 1% digitonin. The inner membrane protein Tim23 and the matrix protein Mge1, as controls for inner mitochondrial compartments, together with Par17 were then monitored during stepwise lysis of mitochondria using digitonin. Identical mitochondrial lysis reactions were performed for all three proteins with increasing amounts of digitonin, analyzed by autoradiography (Par17) or Western blot (Tim23 and Mge1). At a concentration of 0.1% digitonin the inner membrane protein Tim23 was already partially degraded, while Par17 and the matrix protein Mge1 resist PK degradation (Figure 4F). Tim23 is known to display one PK sensitive domain to the inter-membrane space,  which served as an epitope for the antibody used here. All three proteins became susceptible to PK degradation when the mitochondria were lysed completely by 0.2% digitonin.
Therefore, combined data obtained from in vitro experiments with yeast, rat and human mitochondria demonstrate the import of Par17 into mitochondria to be dependent on the presence of the novel N-terminal domain. In addition, Par17 was imported in a time- and membrane potential-dependent manner to the mitochondrial matrix, but without concomitant processing of the protein.
Par17 binds to double-stranded DNA at physiological salt concentrations
Import of Par17 into mitochondria
The recently described protein Par17 differs from the highly conserved parvulin Par14 only by a novel N-terminal domain. In this study, endogenous Par17 was detected in membrane and mitochondrial fractions, and EGFP fusions of Par17 were targeted to mitochondria. The dye used in these experiments (MitoTracker) is only accumulated by mitochondria with intact membrane potential. As no differing mitochondrial staining was detected for transfected cells as compared to untransfected ones, overexpression of Par17 seemed not to be toxic for HeLa cell mitochondria. EGFP fusions of the two Par17 isoforms, Par17-QR and -RS, were targeted to mitochondria with no significant differences in their co-localization values with MitoTracker. Therefore, only one isoform of Par17 (Par17-QR) was used for in vitro import experiments with isolated mitochondria; these experiments clearly showed human Par17 to be imported into the matrix of human, rat and yeast mitochondria. In addition, association of Par17 and Par14 proteins with mitochondrial surfaces was shown irrespective of the origin of the organelles. Consequently, most of the subsequent studies were performed with yeast mitochondria due to their experimental advantages , showing the import of Par17 to be dependent on intact mitochondrial membrane potential and to increase with time. Finally, sub-mitochondrial localization experiments showed that Par17 reached the mitochondrial matrix. Rulten et al  have already detected Par14 within the mitochondrial matrix using an antibody against the PPIase domain. However, as this domain is shared between the two proteins Par14 and Par17, the signal is likely to have originated from Par17 and not Par14.
One puzzling observation was that in vitro imported as well as endogenous Par17 within mitochondrial fractions was not processed after mitochondrial import, which would be expected for a classical targeting peptide. However, non-cleavable presequences have been described for the HSP60 chaperonin 10 , rhodanese , 3-oxoacyl-CoA thiolase  and the beta-subunit of the human electron-transfer flavoprotein . Whether the negative charge of Glu15 within the amphipatic helix prevents this presequence being recognized by the matrix processing peptidase in analogy of yeast Leu9  – another un-processed matrix protein with a glutamate at position 11 – remains to be tested. Even as a non-cleavable presequence, the N-terminal domain was shown to be necessary for import, but the basic and PPIase domains increase mitochondrial targeting.
Mitochondrial proteomic studies
As a mitochondrial protein, Par17 should have previously been detected in one of the recently performed proteomic studies on the constituents of the mitochondrial proteome. Although the yeast mitochondrial proteome has been explored experimentally to about 80% coverage using a variety of mass spectroscopy and tagging techniques [36, 37], neither Par14 nor Par17 are encoded by the yeast genome. Recent proteomic analysis of the yeast mitochondrial proteome (PROMITO) identified two novel PPIases (CPR1, [GenBank:NP_010439] and FPR1, [GenBank:NP_014264])  with no sequence similarities to the parvulins described in this study. Mitochondrial proteomes of mammalian species are assumed to be considerably more complex , and their proportionate coverage by proteomic methods is correspondingly low [40, 41]. The fraction of mitochondrial proteomes resisting identification by large-scale approaches tends to contain proteins that are expressed in low concentrations or only under certain conditions  as well as hydrophobic, small and/or highly basic proteins  with isoelectric points (pI) above 8. With an mRNA level about 50- to 100-fold lower than that of Par14  and a pI of 9.96, Par17 hence represents a mitochondrial, low abundance, basic protein having escaped detection within mitochondria, even in a recent proteomic study on the human mitochondrial DNA nucleoid . Peptides contained in either Par14 or Par17 have not been listed in any of the studies mentioned above.
Evolution of the Par17 prepeptide within anthropoid primates
The proteome of mitochondria has changed dramatically during eukaryotic evolution, with proteins continuously recruited to the mitochondria expanding the proteome of this organelle and providing it with new functions or adaptations . Mitochondrial recruitment can occur by exon duplication and exon shuffling, thereby exchanging existing targeting signals between different nuclear genes  as has been described, for example, for the rps11 gene from Oryza sativa  or the plant cytochrome c 1 precursor .
The contrary seems to be the case with Par17. The evolution of Par17's presequence follows the evolution of primates as can be derived from the DNA sequences obtained in this study. Whereas the coding sequence for Par14 is highly conserved within all primates, the presequence of Par17 evolved steadily from New World monkeys to great apes. There is no spontaneous occurrence of a functional presequence as a result of an exchange with other nuclear encoded genes as this sequence motif is not found anywhere else in the human or macaque genomes. Taken the estimated time scales for primate evolution from Glazko and Nei  and Goodman et al , the insertion leading to the 5' ATG codons to be in-frame with the Par14 coding sequence was acquired not until the separation of Old World monkeys and apes about 23 ± 2 million years ago. Gibbons are known to have separated from great apes about 16 million years ago. The introduction of an interrupting TAA stop codon between the Par17 ATG and the Par14 ATG must have occurred after this branching point, but a more exact time point cannot be concluded from these data. Thus, phylogenetic comparison of genomic sequence data from 10 different primate species confines the Par17 protein to Hominids, only. The presequence therefore represents the most recently evolved functional mitochondrial targeting peptide known to date as it was not acquired until the divergence of apes and Old World monkeys.
Another issue that can be addressed in light of the different genomic Par17 sequences is the occurrence of the different human Par17 isoforms QR and RS. From the alignment in Figure 1A it can be derived that the isoform with Gln16 and Arg18 is the ancestral one. Why humans have acquired these coding SNP and if they serve any function remains to be elucidated in the future.
Could the 5' UTR of parvulin mRNAs have had a function for Par14 expression before it became a coding sequence? The partially conserved upstream ATG codons and short upstream open reading frames might have been involved in translational (down-) regulation of Par14 expression as has been described for other proteins [48, 49]. In addition, differing mRNA secondary structures of the parvulin 5' UTR for several primates were predicted using the MFold prediction server . Within the non-Hominid primate mRNAs, the sequence downstream of the first AUG codon is involved in an extended mRNA stem loop. This putative secondary structure is disturbed by the insertion leading to the sequence found in apes. Therefore, we speculate that mRNA secondary structure and/or translational regulation of Par14 was the evolutionary driving force for this part of the parvulin mRNA before it gained a protein coding function in great apes.
The evolutionary transition from apes to great apes involved significant remodeling of protein constituents of mitochondria: cytochrome c oxidase subunits [51, 52] as constituents of the electron transport chain  as well as ATP synthetase subunits  have undergone accelerated changes during primate evolution. Par17 within the mitochondrial matrix now represents a novel protein constituent added to the Hominid-specific mitochondrial proteome.
Par17 as mitochondrial DNA-binding protein
From the three PPIase families known, cyclophilinD and FKBP-38 have already been shown to be recruited to mitochondria where they play a role at the permeability transition pore and in apoptosis, respectively [55, 56]. However, none of these PPIases is known to bind to double-stranded DNA (dsDNA). The present study however shows that Par17 is able to bind to dsDNA at physiological salt concentrations in a similar way to Par14. Assuming that sequence specificity is not altered between Par17 and Par14, the 16569 base pairs comprising human mitochondrial genome [GenBank:AC_000021] was searched for the following motifs that have been identified as tightest binders by Surmacz et al : dsDNA octamers TAAAAAAT, ATGAAAAT and ATAAAAAT. As octamers, each of these motifs should statistically occur once in 65.5 kB of random sequence. As seven such motifs can be found within the human mitochondrial RefSeq DNA sequence, putative Par17 binding sites seem to be enriched within the mitochondrial genome. A function for Par17 in the context of mitochondrial DNA also seems likely due to its similarities to HMG proteins [13, 15] as mitochondrial HMG proteins have been described very recently .
The DNA binding parvulin protein Par17 is targeted to the mitochondrial matrix by the most recently evolved mitochondrial prepeptide known to-date only occurring in great apes species and man. Brain enlargement in primates has been paralleled by a significant remodeling of the mitochondrial proteome. Hence, a novel protein constituent of the Hominidae specific mitochondrial protein repertoire might have implications on the molecular understanding of brain diseases and the evolution of man.
Phylogenetic analysis of genomic sequences
Genomic DNAs of the following species were obtained from the European Collection of Cell Cultures (ECACC, Salisbury, Wiltshire, UK): Pan troglodytes, Gorilla gorilla, Pongo pygmaeus, Hylobates sp., Papio hamadryas, Macaca mulatta, Chlorocebus aethiops, Aotus trivirgatus and Saguinus oedipus . Human genomic DNA as a positive control was extracted from HeLa cells according to . Genomic DNA from C57 BL6/J mice was kindly provided by Dr Manuela Wuelling (University of Duisburg-Essen, Essen, Germany). All genomic DNAs were amplified with a REPLI-g whole genome amplification kit (Qiagen, Hilden, Germany) prior to PCR. PCRs were performed using the primers 5'-cggctttcaggcatttgtttag-3' and 5'-tttccccgcttttccagaaccact-3' for amplification of exon 1 and 5'-gtcagacacattctatgtgaaaaac-3' and 5'-cccttgcctggctttatcttcact-3' for exon 3, respectively. PCR products were QIAquick gel extracted (Qiagen, Hilden, Germany), TOPO-TA cloned (Invitrogen, Karlsruhe, Germany) and sequenced (GATC Biotech, Konstanz, Germany). Sequences were edited and analyzed using the BioEdit software . The Ensembl genome browser entries from human [Ensembl:ENSG00000102309], macaque [Ensembl:ENSMMUG00000012956], mouse [Ensembl:OTTMUSG00000018308] and chimpanzee [Ensembl:ENSPTRG00000022022] (exon 3 only) were used as reference sequences .
Parvulin exon 1 and exon 3 sequences obtained in this study have been deposited at EMBL with the following accession numbers: Homo sapiens, [EMBL:AM420633]; Pan troglodytes, [EMBL:AM420634]; Gorilla gorilla, [EMBL:AM420635]; Pongo pygmaeus, [EMBL:AM420636]; Hylobates sp., [EMBL:AM420637]; Papio hamadryas, [EMBL:AM420638]; Macaca mulatta, [EMBL:AM420639]; Chlorocebus aethiops, [EMBL:AM420640]; Aotus trivirgatus, [EMBL:AM420641]; Saguinus oedipus, [EMBL:AM420642]. Chlorocebus aethiops is denoted as Cercopithecus sp. within the respective database entry.
Cell fractionation and Western blotting
For cell fractionation, HeLa cells were grown to confluence in petri dishes in medium containing D-MEM, 1× MEM and 1% FCS (all Gibco/Invitrogen, Karlsruhe, Germany) at 37°C and 7.5% CO2, trypsinized and washed in D-MEM. Cell pellets were separated into cytoplasmic, nucleic, membrane and mitochondrial fractions using the Mitochondria Isolation Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions. The nucleic fraction was resuspended in 200 mM Tris-HCl buffer, pH 8.8. Equal amounts of protein (30 μg) were separated on 15% SDS-PAGE gels and blotted on nitrocellulose membranes (Invitrogen, Karlsruhe, Germany) at 100 mA, 35 min. Following 30 min blocking with TBS-T 150 + 2% (w/v) dry milk, membranes were incubated overnight at 4°C with one of the following primary antibodies in TBS-T 150 + 2% milk: rabbit antiserum against the N-terminal Par17 extension (Ab-EXT, , 1:200 dilution); mouse anti-cytochrome c (clone 7H8.2C12, Abcam, 1:200); rabbit HRP-conjugated anti-GAPDH (Abcam, 1:500). The blots were then incubated with the respective HRP-conjugated secondary antibodies and developed using ECL kits (GE Amersham Bioscience, Freiburg, Germany) and CL-XPosure X-ray film (Perbio Science, Bonn, Germany).
Construction and expression of EGFP fusion proteins
Coding sequences of Par14, Par17-QR, Par17-RS, QR presequence, RS presequence, QR presequence + basic domain and RS presequence + basic domain were PCR amplified using the following primers: 5'-aaaaaagaattcgccaccatgccgcccaaaggaaaaagtggt-3' (Par14 forward); 5'-aaaaaaggatcctttcttccttcgaccataataatatg-3' (Par14 and Par17 reverse); 5'-aaaaaagaattcgccaccatgcccatggcggggcttctaaag-3' (Par17 full length and presequence forward); 5'-atatatggatccttggaagcttgttgttgaacgctg-3' (Par17 presequence reverse); 5'-atatatggattcttgggaccttgagccttcttgtca-3' (Par17 presequence plus basic domain reverse). PCR products were EcoRI/BamHI cloned into the pEGFP-N1 vector (Clontech, Saint-Germain-en-Laye, France) and were verified by sequencing.
For expression of parvulin EGFP constructs HeLa cells were grown on cover slips in 12-well plates in HeLa cell medium (as described above) to about 70–80% confluence. Transfections were performed using FuGene (Roche, Mannheim, Germany) according to the manufacturer's instructions and cells were allowed to grow for 40 h. Then the medium was removed and cells were washed in PBS pH 7.4 (Gibco/Invitrogen, Karlsruhe, Germany). For mitochondrial staining cells were incubated in a 100 nM solution of MitoTracker orange (Molecular Probes/Invitrogen, Karlsruhe, Germany) for 30 min, afterwards washed in PBS and fixed in PBS containing 3% paraformaldehyde and 0.2% Triton X-100 for 10 min. Cover slips were washed and mounted on microscope slides.
Image acquisition and data analysis
Image data were retrieved and analyzed with an Olympus BX61 Microscope equipped with bandpass filter cubes, an UPlan SApo 100× Oil objective, an F-View II camera and the Cell^P software (all from Olympus, Hamburg, Germany, and SIS Soft Imaging Systems, Muenster, Germany). Deconvolution of Z-stacks was performed using the nearest neighbor deconvolution algorithm with a haze removal factor of 99%. Processing of the pictures and calculation of co-localization values were all performed within the Cell^P software. In addition, cells were analyzed with a Leica DM IRB Confocal Microscope with TCS SP 2 Confocal Scanner (Leica, Wetzlar, Germany) to judge the quality of the deconvoluted Z-stacks described above.
Isolation of mitochondria
Mitochondria from yeast were isolated from wild type strain PK82 (MAT α, his4-713, lys2, ura3-52, trp1, leu2-3;  grown in YPG medium (1% (w/v) yeast extract, 2% (w/v) bacto-peptone, pH 5.0, containing 3% (v/v) glycerol) following standard procedures [28, 30]. Rat liver and Jurkat cells mitochondria were obtained essentially as published previously [62, 63].
Import of proteins into isolated mitochondria
Radiolabeled Par17-QR, -RS, Par14 and control proteins were synthesized in rabbit reticulocyte lysate (TNT T7 Coupled Reticulocyte Lysate System, Promega, Mannheim, Germany) in the presence of 35S-labeled methionine (ICN Biomedical Research Products, Eschwege, Germany). In sample sizes of 50–100 μl the radiolabeled proteins were imported into isolated mitochondria as described previously . Under standard conditions, the import assay contained BSA buffer (3% (w/v) BSA, 80 mM KCl, 10 mM MOPS-KOH, pH7.2), 2–5 μl reticulocyte lysate, 2 mM NADH, 1 mM ATP, 20 mM potassium phosphate and yeast mitochondria (30 μg mitochondrial protein). As import into isolated mammalian mitochondria is generally less efficient compared to yeast mitochondria [28, 64], mammalian mitochondria were added at a higher concentration (40 μg mammalian mitochondrial protein). The import reactions were carried out at 25°C and protein uptake stopped by incubation at 0°C. Non-imported proteins were either degraded by addition of proteinase K at a final concentration of at least 100 μg/ml and incubation for 10 min at 0°C or the samples were left untreated. The protease was inactivated by addition of 2 mM PMSF and additional incubation for 5 min at 0°C. Eventually, the mitochondrial membrane potential was dissipated by addition of valinomycin (Sigma, Munich, Germany) at a final concentration of 1 μM from a 100-fold concentrated stock solution in ethanol. Selective opening of the mitochondrial outer membrane was achieved by incubation of mitochondria in EM buffer (1 mM EDTA, 10 mM MOPS-KOH, pH 7.2) for 20 min at 0°C (swelling). Alternatively, digitonin (Calbiochem/Merck, Darmstadt, Germany) was added up to a final concentration of 0.1% (w/v). For association studies, mitochondria were separated from soluble protein by the addition of SEM buffer (500 mM sucrose, 1 mM EDTA, 10 mM MOPS, pH 7.2) and subsequent centrifugation. An additional washing step in a 250 mM sucrose containing SEM buffer was performed to ensure efficient separation of mitochondria from lysate ingredients.
DNA binding assay
HeLa cells were transfected with Par14-, Par17-RS- and Par17-QR-EGFP fusion constructs as described above and lysed in 50 mM K-PO4, 1 mM EDTA, 0.1% Triton X, 1 mM Pefablock (Roth, Karlsruhe, Germany), pH 7.0. Cells were filled up to a total volume of 4 ml with dilution buffer (20 mM Tris-HCl, pH 7.5, 10% glycerol, 1 mM EDTA, 1 mM DTT, 0.1% Triton X, 37.5 mM (NH4)2SO4), disrupted by ultrasonic and centrifuged to remove debris (20 min, 4°C, 4000 × g). DNA cellulose (0.25 g calf thymus DNA cellulose (Sigma, Munich, Germany)) was suspended in 3 ml binding buffer (20 mM Tris, pH 7.5, 10% glycerol, 100 mM KCl, 1 mM EDTA, 10 mM NaF and 0.1% Triton X) and incubated rotating at 4°C overnight. A total of 850 μl of cellulose suspension was transferred to a 2 ml microcentrifuge tube, washed with fresh binding buffer and centrifuged (10 min, 4°C, 2000 × g) three times. The supernatant was then replaced by 850 μl of cell lysate. The suspension was incubated with rotating for 1 h at 4°C and then centrifuged (2 min, 10000 × g, 4°C). The supernatant was stored at 4°C. The cellulose was washed successively with binding buffer containing increasing concentrations of KCl. After each washing step the suspension was incubated and centrifuged as described above. All supernatants were methanol/chloroform precipitated, run on 12.5% SDS-PAGE gels and analyzed by Western blotting using anti-GFP antibodies as described above. Blots were analyzed quantitatively by densitometry using Scion Image software .
The experiments were repeated using radiolabeled proteins instead of EGFP fusion constructs. Here, 50 μl of cellulose suspension and 5 μl of cell lysate as well as 50 μl of the different binding buffers were sufficient. All incubation times were reduced to 20 min. Gels were analyzed autoradiographically as described above.
enhanced green fluorescent protein
peptidyl prolyl cis/trans isomerase
single nucleotide polymorphism
high mobility group (protein)
- Par17-QR and -RS:
(human) parvulin 17 with either Gln16+Arg18 or Arg16+Ser18
We thank Dr Matthias Rabiller as well as Nina M Link for fruitful discussions and Simone Bischof for technical assistance. Dr Manuela Wuelling is acknowledged for providing mouse genomic DNA. This work was supported by Deutsche Forschungsgemeinschaft (DFG) grant BA1624/8-1 (to PB and DK) and DFG Sonderforschungsbereich SFB 495 Stuttgart/Hohenheim.
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