Cell cycle and cell death are not necessary for appressorium formation and plant infection in the fungal plant pathogen Colletotrichum gloeosporioides
© Nesher et al; licensee BioMed Central Ltd. 2008
Received: 06 June 2007
Accepted: 14 February 2008
Published: 14 February 2008
In order to initiate plant infection, fungal spores must germinate and penetrate into the host plant. Many fungal species differentiate specialized infection structures called appressoria on the host surface, which are essential for successful pathogenic development. In the model plant pathogen Magnaporthe grisea completion of mitosis and autophagy cell death of the spore are necessary for appressoria-mediated plant infection; blocking of mitosis prevents appressoria formation, and prevention of autophagy cell death results in non-functional appressoria.
We found that in the closely related plant pathogen Colletotrichum gloeosporioides, blocking of the cell cycle did not prevent spore germination and appressoria formation. The cell cycle always lagged behind the morphogenetic changes that follow spore germination, including germ tube and appressorium formation, differentiation of the penetrating hypha, and in planta formation of primary hyphae. Nuclear division was arrested following appressorium formation and was resumed in mature appressoria after plant penetration. Unlike in M. grisea, blocking of mitosis had only a marginal effect on appressoria formation; development in hydroxyurea-treated spores continued only for a limited number of cell divisions, but normal numbers of fully developed mature appressoria were formed under conditions that support appressoria formation. Similar results were also observed in other Colletotrichum species. Spores, germ tubes, and appressoria retained intact nuclei and remained viable for several days post plant infection.
We showed that in C. gloeosporioides the differentiation of infection structures including appressoria precedes mitosis and can occur without nuclear division. This phenomenon was also found to be common in other Colletotrichum species. Spore cell death did not occur during plant infection and the fungus primary infection structures remained viable throughout the infection cycle. Our results show that the control of basic cellular processes such as those coupling cell cycle and morphogenesis during fungal infection can be substantially different between fungal species with similar lifestyles and pathogenic strategies.
Fungal spores are resting structures that efficiently disseminate the fungal organisms from which they originate. Therefore, spores are programmed to germinate only under appropriate conditions, which vary between species according to lifestyle and nutritional requirements. In plant pathogenic fungi, germination is frequently stimulated by plant-specific signals, such as plant-derived compounds or the physicochemical properties of the plant surface. In the genus Colletotrichum, spores are affected by self-inhibitory compounds that prevent germination in dense populations [1, 2], but germination and appressorium formation can be triggered by specific external signals such as cuticular waxes or hard hydrophobic surfaces [3–5]. Spore germination in soil-borne fungi is enhanced by root exudates, whereas in rust fungi the direction of germ tube growth and appressoria formation are sensitive to the properties of the leaf surface [6, 7].
Fungal spores must complete several developmental stages on the host surface before they can penetrate into host tissues. The sequence of events includes activation of metabolism, germ tube initiation, a short period of polar growth coupled with a limited number of cell divisions, polar growth arrest, and differentiation of an appressorium. Since the surface of a leaf lacks most nutrients, completion of the pre-penetration development depends primarily on the spore's endogenous resources and involves degradation and recycling of stored lipids, carbohydrates, and nitrogen sources . It is presently unclear whether the limited growth of the pathogen on the host surface is restricted by developmental programs, by nutritional limitations, or by both.
Studies of the model plant pathogen Magnaporthe grisea showed that spore carbon and lipids sources are degraded during germination or translocated into the developing appressorium [9, 10]. Upon contact with a host surface, spores of M. grisea form a short germ tube and then differentiate a dome-shaped appressorium that generates high turgor pressure by accumulating high levels of glycerol [11, 12]. Early studies showed that the spore and germ tube collapsed following appressorium formation and it has been suggested that the spore and germ tube cytoplasm move into the appressorium [12, 13]. Thines et al  showed a mass transfer of spore carbohydrates and lipid bodies into the young appressorium that is controlled by PMK1 MAP kinase and cAMP pathways. Recent work has presented evidence that spores of M. grisea undergo autophagic cell death following mitosis and appressorium formation . Completion of mitosis and the autophagy cell death were found essential for appressorium formation and appressorium mediated penetration, respectively; mutants that were unable to undergo mitosis could not produce appressoria, while autophagy mutants mgatg8 and mgatg1 differentiated appressoria, but were unable to penetrate into the host plant [14, 15]. Similarly, in Colletotrichum lindemuthianum, mutants in CLK1 (a homolog of MgATG1) are also unable to cause disease owing to the inability of appressoria to penetrate the host cuticle 
Colletotrichum gloeosporioides f. sp. aeschynomene is a hemibiotroph plant pathogen infecting the legume plant Aeschynomene virginica. It shares similar lifestyles and infection strategies with M. grisea, particularly during the early stages of pathogenesis. Spores quickly germinate on the host surface, form a short germ tube, and then differentiate well-developed, melanized appressoria. These appressoria generate high turgor pressure and then differentiate infection pegs that penetrate the plant cuticle by mechanical force. We previously showed that C. gloeosporioides can germinate in two distinct ways: on plants or on a hydrophobic surface spores undergo mitosis and then develop a single germ tube . This process is initiated immediately following induction of germination and terminates within less than 4 h, with the formation of appressoria. In rich media, spores germinate as described in Aspergillus ; upon induction, the spore swells, and after several hours it forms a germ tube and then a second germ tube develops from the other side of the spore. Such a germination process does not lead to the formation of an appressorium and these spores have reduced pathogenicity. We have defined these two germination processes as 'pathogenic' and 'saprophytic', respectively . The two germination processes in C. gloeosporioides are regulated by different signaling cascades: saprophytic germination is enhanced by cAMP, whereas pathogenic germination is cAMP-independent. Similar to M. grisea, cAMP is required later for appressoria formation.
In this work we have characterized the sequence of events occurring during pathogenic spore germination using a nucleus green fluorescent protein (GFP)-tagged strain. We found that inhibition of the cell cycle did not prevent spore germination, and that mitosis was not required for appressorium differentiation. We also found that spores, hyphae, and appressoria retained intact nuclei and were viable for over 72 h post plant inoculations. These results are in sharp contrast to the situation described in M. grisea . Importantly, our results indicate that even in closely related plant pathogens that use similar infection strategies to invade plant tissue, regulation of the infection process is very different.
Nuclear division and germ tube formation
Additional file 1: Time lapse microscopy of first nuclear division during spore germination (S1.wmv). Note that the first nuclear division occurs before germ tube formation. Pictures were taken every 3 min, at 40× magnification. (WMV 673 KB)
Spore germination can occur without mitosis
Effect of HU, benomyl, and LatA on spore germination. Spores were germinated on glass slides in PE. The percentage of spore germination was determined after 3 and 6 h. HU data is the average of four independent experiments. Benomyl and LatA experiments were repeated three times with two repeats in each experiment. Similar results were obtained in each of the three independent experiments.
89 ± 7.5
93 ± 6
46 ± 25
85 ± 6
65 ± 2
87 ± 1
98 ± 1
Nuclear division and appressorium formation
The effect of HU on germination and appressoria formation in five different Colletotrichum species. Fungi were germinated on the surface of a Petri dish in water (C. magna, C. coccodes, C. acutatum) or in PDB medium (C. gloeosporioides, C. musae). Spore concentration was 105/ml in all species. Percentage germination and appressoria formation were determined after 7 h (C. gloeosporioides, C. coccodes), 9 h (C. acutatum, C. musae), or 13 h (C. magna). In each sample 150 spores were counted, with two independent samples per experiment. Appresoria percentage is the percentage of germ tubes that formed appressoria.
H 2 O
H 2 O
83.5 ± 7.5
77 ± 3
91.5 ± 6
45.5 ± 12.5
95 ± 3
75 ± 10
80 ± 15
73 ± 3
47 ± 1
33 ± 2
94 ± 2
86.5 ± 6.5
35.5 ± 5.5
25.5 ± 7.5
63.5 ± 15
26.5 ± 22
66 ± 1
50 ± 25
Nuclear division occurs in appressoria after the completion of plant penetration
We monitored the development of appressoria and infection hyphae on onion epidermis. Following the migration of a nucleus into the appressorium, a septum formed that separated the appressorium from the germ tube (Figure 1f). During appressorium maturation, which can last up to 24 h, no further nuclear division could be detected in the appressoria, suggesting that cell cycle progression was arrested. Upon plant penetration, nuclear division within the appressorium was resumed, one of the nuclei migrated into the penetration hypha, whereas the other nucleus remained inside the appressorium (Figure 3Cb,c). Further mitoses followed as primary hyphae developed inside the plant tissue (Figure 3Cd). This result is in sharp contrast to M. grisea, in which the nucleus migrates from the appressorium into the penetrating hypha and then undergoes mitosis just before the development of the invasive hypha .
Spores and appressoria remain viable following plant invasion
Plant pathogenic fungi devised various strategies and mechanisms to infect plants. However, regardless of the pathogenic lifestyle, the initial stages of infection are common to most fungi: spores germinate on the host surface, undergo a short period of polarized growth, and then develop appressoria, which direct the growth into the plant tissues. Since the plant surface is poor in nutrients, it is assumed that fungi must utilize the spore's endogenous reserves to quickly move from the plant surface into the inner tissues. Various signals such as cutin monomers or surface physical features are known to induce appressoria formation, but it is unclear at which point post germination this switch from polar growth to swelling of the tip will occur. The short period of growth on the host surface is characterized by tight coupling of morphogenesis and cell cycle progression. On surfaces that support appressoria formation such as on plants or on a hydrophobic surface, appressoria will form on short germ tubes after a few nuclear divisions. On surfaces that do not support appressoria formation the germ tube will elongate and undergo many more nuclear and cell divisions. Thus, the number of cell divisions is not used by the fungus to determine the stage of appressorium formation without an appropriate surface signal. In this work we have studied the association between nuclei division and morphogenesis during pathogenic spore germination, appressorium formation, and appressorium-mediated plant penetration using a C. gloeosporioides transformant expressing Histone1-eGFP fusion protein.
Analysis of nuclear divisions revealed that mitosis was initiated immediately following exposure to germination-inducing signals. The first mitosis was observed 90 min after induction, which is similar to the elapsed time between the first and second mitoses. This suggests that in C. gloeosporioides, growth and cell division are inhibited in spores, but the spores are not dormant and can readily resume growth without a preceding adaptation stage upon receiving the proper signals. The growth inhibition is attributed to self-inhibitory compounds in the spore cell wall since removal of these substances by washing of the spores can enhance spore germination even without specific induction [1, 2]. The instant mitosis triggered during pathogenic germination differs from the pattern observed during saprophytic germination in C. gloeosporioides and other fungal species in which breaking of dormancy and isotropic swelling precedes mitosis and growth . In Candida albicans, cells that grow to high densities are arrested in G1. When diluted to lower densities the cells resume growth, but the first cell cycle is characterized by an extended G1 phase, which is about 70 min longer than the G1 phase in dividing cells . In Aspergillus nidulans, spores are dormant and germination is preceded by an early stage that lasts several hours and includes activation of metabolism and isotropic swelling [17, 22]. In C. gloeosporioides, saprophytic germination includes an initial period during which spores start swelling, but are neither growing nor dividing . In contrast, pathogenic germination has no adaptation stage; mitosis is tightly linked with germination response and seems to occur simultaneously or prior to cell growth.
We used a pharmacological approach in attempting to separate between the cell cycle and cell growth. Treatment of cells with HU always prevented the first nuclear division. This result indicates that in C. gloeosporioides the spores were arrested at G1, similar to the situation in other species [18, 23, 24]. The continuation of germination without mitosis is also in agreement with results from other species. In F. graminearum nuclear division is not required for spore germinating in rich medium . Under similar conditions, A. nidulans spore germination is tightly coordinated with nuclear division, but these two processes can be uncoupled under less favorable conditions . In Aspergillus fumigatus, cell cycle and morphogenesis are not interdependent, and it has been suggested that similar to yeast, these two processes run in parallel but share common checkpoints . Taken together, it seems that in most cases initiation of germination is independent of the cell cycle.
Blocking of mitosis in germinating spores of M. grisea using HU or by genetic intervention also lead to germination without nuclear division; however, appressoria formation was prevented . Similar results were reported in C. trancatum, where inhibition of the second round of DNA synthesis did not affect spore germination but blocked appressoria formation . In contrast, C. gloeosporioides spores continued to grow for at least 7–8 h in presence of HU, forming normal numbers of well-developed appressoria. Treatment of spores with benomyl also had no effect on germination rates, but growth was retarded at an earlier stage before appressoria formation. The early growth retardation following benomyl treatment is attributed to the role of microtubules in growth rather than to the lack of nuclei separation . These results suggest that in C. gloeosporioides all stages of pathogenic germination, including appressoria formation, are independent of mitosis, unlike M. grisea and C. trancatum in which appressoria formation and pathogenic development depend on completion of mitosis [14, 27]. Several other Colletotrichum species that we tested also produced high rates of appressoria in presence of HU, which were only slightly lower than the percentage of appressoria formation in untreated spores (Table 2). We noted that all of these species have small, unicellular spores whereas both M. grisea and C. trancatum have larger, three cells spores. At this point we do not know whether this structural difference has anything to do with the differences that we found in the regulation of cell cycle and appressoria formation.
We previously suggested a model in which plant signals bypass the effect of the self-inhibitory compounds and activate both mitosis and morphogenesis . Our current results suggest that induction of germination is unrelated to the induction of the cell cycle. This implies that either these two processes run in parallel and are simultaneously induced by the same signals, or that they might respond to different stimuli. In order to address these different possibilities, we used LatA to block cell growth and determined the effect on nuclear division. Unlike HU, which only blocked mitosis, LatA completely blocked both spore germination and nuclear division. When LatA was removed by washing of the spores, germination was resumed, showing that the effect was not a result of the toxicity of LatA but rather of the specific disruption of the actin cytoskeleton (data not shown). Thus, prevention of polar growth also leads to cell cycle arrest.
Additional file 2: Time lapse microscopy of second nuclear division during conidial germination (S2.wmv). Second nuclear division occurs during germ tube elongation. Pictures were taken every 3 min, at 25× magnification. (WMV 673 KB)
According to these new results, we propose that plant signals specifically activate germination and that the cell cycle is induced only after initiation of germination. According to this model, the self-inhibitory compounds only inhibit growth, whereas cell cycle arrest is not directly affected by these compounds. This sequence of events is opposite from that in M. grisea, in which mitosis must be completed before appressorium formation, and the nucleus within the appressorium does not divide but migrates from the appressorium through the penetration peg into the primary hyphae . These differences are surprising, especially because M. grisea and C. gloeosporioides share a very similar infection strategy. Recently, Kankanala et al.  reported that M. grisea infects plant cells by moving biotrophic hyphae from one cell to another. This mode of infection differs from the infection mode of the true hemibiotroph Colletotrichum species, including C. gloeosporioides which use biotrophic hyphae to invade a few cells and then differentiate necrotrophic hyphae that kill the host cells . These differences, together with the difference in regulation of the early stages of infection, indicate that despite the apparent similarities in the sequence of events leading to pathogenesis, these fungi must have evolved very different regulatory mechanisms to control pathogenesis.
Another striking difference between C. gloeosporioides and M. grisea was our finding that the spores and appressoria of C. gloeosporioides remained viable throughout the infection cycle, unlike the spores of M. grisea, which were subjected to autophagy cell death soon after appressorium formation . This result is especially surprising since the unicellular spores of C. gloeosporioides are much smaller and therefore contain fewer resources than the three-celled spores of M. grisea. In several other fungal species it has been shown that spore components, and especially lipids and carbohydrates are recycled during germination . These works suggest that recycling of the spore components during germination is necessary for progression of the early stages of fungal development. In M. grisea mutants in the MAP kinase PMK1 and mitosis-defective mutants that are unable to form appressoria keep growing on the leaf surface without loosing viability . Additional M. grisea mutants also show this phenomenon, including G proteins and cAMP pathway mutants [31–33]. Moreover, in other fungi, mutants in similar genes were also able to develop considerable amounts of mycelia on the leaf surface [34, 35]. In C. gloeosporioides, spores that are germinated in rich medium do not develop appressoria and are not infective, but keep developing on the leaf surface . Collectively, these results show that when regulation of early pathogenesis is interrupted, fungal spores are capable of growing on the leaf surface for much longer periods of time than the very limited number of nuclear divisions, which normally precede appressoria formation. Therefore, the number of nuclear divisions prior to appressoria formation is not restricted by nutrient availability but rather, the limited growth of germ tubes before appressoria formation seems to constitute a common element in the regulation of early fungal pathogenesis. When the signal for growth arrest is missing, owing to mutations or lack of appropriate signals (e.g. on a hydrophilic surface or in rich medium), polar growth continues and mycelium develops. The endogenous nutrients within spores should be sufficient to support production of this biomass, or fungi must be able to absorb nutrients without plant penetration. Although we presently have no answers to these questions, we assume that both possibilities might occur. The final behavior of the spore, either for life or death, seems to be of variable importance; in some cases such as in M. grisea, it might be important, whereas in other cases such as in C. gloeosporioides, programmed cell death does not occur and is not required for plant infection.
Spores of C. gloeosporioides are arrested at G1, but are not dormant. Germination signals activate cell polarization, and although growth and cell cycle occur in parallel, appressoria formation is independent of mitosis. Other Colletotrichum species with unicellular spores can also form appressoria without mitosis, suggesting that this phenomenon is not unique to C. gloeosporioides. Another striking difference between C. gloeosporioides and M. grisea is our finding that the spores and appressoria of C. gloeosporioides remain viable throughout the infection cycle. Thus, despite the apparent similarities in pathogenic development, these fungi must have evolved very different regulatory mechanisms to control appressoria formation and host infection. The limited growth of germ tubes constitutes a common element in the regulation of early fungal pathogenesis. The development on the leaf surface is restricted by regulatory signals, but is not limited by nutrients availability, suggesting either that the endogenous nutrients are sufficient to support production of a relatively large fungal biomass, or that fungi can obtain external nutrients even before plant penetration.
Fungi, plants, and growth conditions
Colletotrichum gloeosporioides f. sp. aeschynomene 3.1.3 wild-type and transgenic strains were used. Emerson's YpSs (EMS) solid media and pea extract (PE) were prepared as described previously . Inoculation experiments were conducted with onion epidermis. All of the experiments were conducted in a growth chamber at 28°C.
Germinating spores were transformed as described previously . Transgenic strains that express the Histone1-eGFP fusion protein were obtained by co-transformation of the pMF-280 plasmid , which carries the Histone1-eGFP gene fusion under the CCG-1 promoter, and the p57-GPD-HPH plasmid, which carries the HPH resistance gene under the A. nidulans GPDA promoter.
Spores were harvested from 5-day-old EMS plates in ddH2O, then counted, and diluted to 106 spores/ml in PE. Germination assays were performed on hydrophobic (Petri dish) or hydrophilic (glass slide) surfaces, using 30-, 50-, or 100-μl droplets. Plant inoculation assays were performed with 10-μl droplets of 105 spores/ml water suspensions with 0.01% Tween-20 (Sigma).
Cell cycle and cytoskeleton
HU (Sigma) was prepared as a 1 M stock solution in ddH2O and used for blocking of cell cycle, at a final concentration of 30 mM. Benomyl (Sigma) was prepared as a 1 mg/ml stock solution in 95% ethanol and used to inhibit nuclei separation and blocking of the cell cycle, at a final concentration of 5 μg/ml. LatA (Calbiochem) was prepared as a 1 mM stock in dimethylsulfoxide (DMSO) and used to disrupt actin microfilament polymerization, at a final concentration of 5 μM. Compounds were diluted in PE medium.
Apoptosis was induced by treating the fungus with lovastatin (Calbiochem). Lovastatin was prepared as a 6-mM stock solution in ethanol and added to spores two hours after induction of germination, at a final concentration of 250 μM. Apoptosis was determined by the TUNEL assay using the In Situ Cell Death Detection kit, Fluorescein (Roche Diagnostics, Germany) as described previously .
Staining and microscopy
FDA staining was performed as described previously . Fluorescent and light pictures were taken using LSM 510 confocal microscope. Excitation and emission wavelengths for FDA and GFP were 493/510 nm and 488/505 nm, respectively.
We wish to thank Michael Freitag for the H1:eGFP plasmid. This work was supported by The Israel Academy of Sciences Grant # 722/01 and by the DFG grant # Tu 50/9-3.
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