A question of scale: Human migrations writ large and small
© Cox and Hammer; licensee BioMed Central Ltd. 2010
Received: 19 April 2010
Accepted: 13 July 2010
Published: 21 July 2010
Several recent papers illustrate the importance of migration and gene flow in molding the patterns of genetic variation observed in humans today. We place the varied demographic processes covered by these terms into a more general framework, and discuss some of the challenges facing attempts to reconstruct past human mobility and determine its influence on our genetic heritage.
Migration is a major force shaping patterns of genetic variation in humans . The history of our species is dominated by movement: from the global expansion of modern humans out of Africa, to continent-wide dispersions following the development of agriculture, to the often socially dictated movement of individuals seeking mates or resources. As can be seen from even these simple examples, migration can occur over a variety of spatial and temporal scales - from the wholesale dispersal of populations to the movements of individuals. Large-scale dispersals cause populations to diverge as they subsequently accumulate mutations and experience genetic drift, while individual mobility over smaller geographical scales counteracts these forces by sharing genetic variants between populations. Thus, each of these forms of migration has characteristic effects on human population structure and each brings its own set of methodological challenges [2, 3].
In practice, it remains difficult to determine the timing and geographic extent of specific episodes of historical human migration. Spatial control largely reduces to an issue of appropriate sampling (that is, sufficiently representing the full geographical range over which migration occurred). Increased geographical sampling is currently an expensive proposition, but advances in genotyping technologies are likely to reduce experimental costs to more reasonable levels in the near future. Attaining fine temporal control is a far more difficult prospect. To date, tight temporal resolution of human mobility has largely been the sole purview of ancient DNA studies. However, new analytical methods are currently being developed that can place chronological bounds on human gene flow using genetic data from individuals living today. The choice of genetic marker is also a major influence on what researchers can reveal about historical movements. Until recently, mitochondrial DNA (mtDNA) and the Y chromosome were favored as the most informative genetic markers available for studying human history. mtDNA is passed only from a mother to her offspring, while the Y chromosome is passed only from a father to his sons. This simple mode of transmission makes mtDNA and the Y chromosome easy model systems for studying human ancestry. In particular, these loci can be used to detect whether migration has occurred between populations in the past.
The authors of a recent article in BMC Biology adopted exactly this approach. Li and colleagues  report mitochondrial and Y chromosome data for 23 inhumations at the Xiaohe cemetery in Western China's Tarim Basin. Here, ancient DNA techniques were employed to characterize the genetic diversity of samples fixed in both time and space. The Xiaohe community existed around 4,000 years ago on the ancient east-west network of trade routes known collectively as the Silk Road. Despite straddling the border of East Asia, 'Caucasoid' mummies, artwork influenced by ancient western civilizations and records written in Tocharian, an extinct branch of the Indo-European language family, amply illustrate the region's close connections with the west. Li and colleagues contribute to this extensive evidence of east-west links by announcing the presence of West Eurasian mtDNA and Y chromosome lineages among Xiaohe's dead.
However, mtDNA and Y chromosome records are necessarily biased towards the sex-specific movements of women and men, respectively. Further, demographic reconstructions made from mtDNA and the Y chromosome have considerable uncertainty due to the substantially higher rates of stochastic genetic drift in these uniparentally inherited systems compared to autosomal loci. Ultimately, mtDNA and the Y chromosome are just two small loci in a vast ocean of human genetic variation. In contrast, the autosomes consist of millions of independently evolving regions, any number of which can be surveyed to characterize gene flow in humans. Today, the rapid advent of new genotyping technologies is allowing massive numbers of markers to be screened across the human genome. Xu and colleagues  adopted this approach in a recent article in BMC Genetics. They report approximately 50,000 single nucleotide polymorphisms (SNPs, or point mutations) drawn from the nuclear genome. These markers were genotyped in individuals from two indigenous communities in Thailand that show linguistic and anthropological evidence of prehistoric connections. Using a suite of clustering methods, Xu and colleagues demonstrate that the Mlabri and Htin share more nuclear variants with each other than either does with surrounding populations.
While determining whether two populations share genetic variation is a relatively simple exercise, identifying and quantifying the amount of gene flow between them requires more advanced modeling and inferential statistics. This is usually applied within a framework of coalescent theory. Large quantities of genetic information are required to infer rates of gene flow, and data sets of this size have only recently become feasible. In a 2008 article in BMC Genetics, we adopted exactly this sort of strategy to quantify rates of gene flow on a global scale . Instead of screening many pre-ascertained point mutations like Xu and colleagues, we instead fully sequenced 20 large genomic regions distributed across the human X chromosome in 90 individuals from six globally distributed populations (Mandenka, Biaka and San in Africa, and French Basques, Han Chinese and New Guinea Highlanders in Eurasia). This research was unique because these 20 genomic regions were chosen specifically to be recombinationally unlinked (that is, independent) and selectively neutral (that is, located far away from genes), in marked contrast to most studies where genetic variants are tightly linked to functional sites that are potentially affected by natural selection (for example, mtDNA and the Y chromosome) or even occur within selected loci (for example, SNP chip data where many polymorphisms are located in, or close to, genes). We found that worldwide rates of gene flow (m) were approximately five-fold higher among non-African populations relative to African groups . Interestingly though, effective population sizes (N) and migration rates (m) are inversely proportional in African and Eurasian groups - although migration rates are approximately five-fold lower in Africa, effective population sizes are approximately five-fold higher. Consequently, population migration rates (Nm) are globally very similar (Nm = 2.4). In other words, we found that approximately two migrants per generation have moved between these globally distributed populations on average through time. Some of these movements may have been recent, despite the large geographical distances between populations, but most would have occurred when these populations were geographically much closer (for example, during the initial expansion of anatomically modern humans out of Africa). Developing statistical methods to determine exactly when this gene flow occurred remains an important outstanding task.
Today, sequencing and comparing entire human genome sequences is the surveying method of choice. For example, the complete genomes of many individuals have now been sequenced, including such famous names as Archbishop Desmond Tutu  and Nobel Laureate James Watson . Genome sequences yield orders of magnitude more information than either the SNPs screened by Xu and colleagues, or the small genomic regions (comparatively speaking) sequenced in our 2008 research program.
Gene flow from Neandertals
Although this particular episode of gene flow has been characterized with good spatial and temporal resolution, similar detail is rare in other studies of human gene flow. Indeed, many questions still remain about how gene flow has helped shape patterns of genetic variation in humans. mtDNA and the Y chromosome are just two small genetic regions within a very large genome, and as the Neandertal study shows, they are not necessarily representative of much of our genetic diversity largely due to their increased rates of genetic drift. Furthermore, sampling ancient DNA becomes increasingly difficult at ever greater time depths - at 4,000 years old, the Xiaohe cohort stretches back less than one-tenth of the continental history of Eurasia, a region first settled after 50,000 years ago. The Neandertal study does reach back this far, but reflects a contact event that was probably over quickly; by approximately 40,000 years ago, modern humans were dominant across Eurasia, and Neandertals were on the wane. For most other questions involving human movements, reconstructing an inclusive spatial and temporal history will depend on the vast genomic datasets now being produced for a wide range of populations. In tandem, improved inference methods must be developed to attain this goal. With the exception of ancient DNA studies, current rates of global gene flow are all long-term estimates (that is, migration rates are averaged over very long time spans). Our inability to resolve the timeframes over which specific demographic processes occurred is a major limitation that requires further attention.
Finally, we note that human migration cannot simply be characterized by the timing and geographical extent of gene flow. Human mobility is far more nuanced, and at least four additional axes would seem to warrant further consideration. First, rates of gene flow are hard to determine with certainty. Although the presence (or absence) of migration can often be inferred relatively easily, estimates of actual rates frequently have considerable uncertainty . Methods allowing better resolution of rates of gene flow are required. Second, migration can be sex specific. Although movement into the Xiaohe community was apparently not sex biased , sex specific mobility has been observed elsewhere. For instance, the spread of Asian alleles into Island Southeast Asia appears to have favored Asian women over Asian men . Adequate sampling of sex-linked markers (for example, X chromosome versus autosomes) is a prerequisite for detecting such patterns. Third, gene flow can be directional (for example, movement along the Silk Road) or non-directional (for example, isolation-by-distance). Directional mobility along the Silk Road will produce quite different genetic patterns than intermarriage between adjacent communities. Improved regional sampling will be necessary to detect such patterns. Fourth, rates of gene flow may change over time. Varying rates of gene flow strongly affect patterns of genetic diversity , although to our knowledge, no statistical methods have yet been developed to detect real-world examples of these processes.
The series of migration vignettes presented in this commentary illustrates just how little we understand about the role of migration and gene flow in human history. Recent studies have shed some light on the processes affecting movement among communities, between continents and across the globe, but more sophisticated methods are needed. We look forward to meeting these methodological challenges and the clarity that a more nuanced understanding of human mobility will bring.
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