The conservation and uniqueness of the caspase family in the basal chordate, amphioxus
- Liqun Xu†1,
- Shaochun Yuan†1,
- Jun Li1,
- Jie Ruan1,
- Shengfeng Huang1,
- Manyi Yang1,
- Huiqing Huang1,
- Shangwu Chen1,
- Zhenghua Ren1 and
- Anlong Xu1Email author
© Xu et al; licensee BioMed Central Ltd. 2011
Received: 4 May 2011
Accepted: 21 September 2011
Published: 21 September 2011
The caspase family, which plays a central role in apoptosis in metazoans, has undergone an expansion in amphioxus, increasing to 45 members through domain recombination and shuffling.
In order to shed light on the conservation and uniqueness of this family in amphioxus, we cloned three representative caspase genes, designated as bbtCaspase-8, bbtCaspase-1/2 and bbtCaspase3-like, from the amphioxus Branchiostoma belcheri tsingtauense. We found that bbtCaspase-8 with conserved protein architecture is involved in the Fas-associated death domain-Caspase-8 mediated pro-apoptotic extrinsic pathway, while bbtCaspase3-like may mediate a nuclear apoptotic pathway in amphioxus. Also, bbtCaspase-1/2 can co-localize with bbtFADD2 in the nucleus, and be recruited to the cytoplasm by amphioxus apoptosis associated speck-like proteins containing a caspase recruitment domain, indicating that bbtCaspase-1/2 may serve as a switch between apoptosis and caspase-dependent innate immune response in invertebrates. Finally, amphioxus extrinsic apoptotic pathway related caspases played important roles in early embryogenesis.
Our study not only demonstrates the conservation of bbtCaspase-8 in apoptosis, but also reveals the unique features of several amphioxus caspases with novel domain architectures arose some 500 million years ago.
Programmed cell death is a gene-guided process for the elimination of unnecessary or harmful cells in which the cysteine proteases caspases are core elements [1–3]. To date, 11 members of this family have been identified in the human genome [4, 5], caspase-1 to caspase-10, and caspase-14. They can be divided, with respect to function, into two major groups. The first, including the initiator caspases (caspase-2, -8, -9 and -10) and the effector caspases (caspase-3, -6 and -7), lead directly to apoptosis , while the others, including caspase-1, -4 and -5, are involved in the maturation of pro-inflammatory cytokines . Caspase-14 is mainly expressed in the epidermis and takes part in epidermal barrier formation .
In vertebrate cells, the apoptotic response is mediated through either the intrinsic or extrinsic pathway. The intrinsic pathway is triggered by death stimuli generated within the cell, such as DNA damage, leading to the release of mitochondrial cytochrome c, which associates with caspase-9 and apoptotic protease activating factor 1 (Apaf-1) to form an apoptosome . The extrinsic apoptotic pathway is initiated by the binding of extracellular death ligands to death receptors (DRs), such as FasL binding to Fas. As members of the TNF receptor (TNFR) superfamily, the DRs further recruit the cytosolic adaptor Fas-associated death domain (FADD), which interacts with procaspase-8 through death effector domains (DEDs) to form an oligomeric death-inducing signaling complex . The activated caspase-8 released subsequently cleaves the effector caspase-3, which finally executes apoptosis . In addition to apoptosis, caspase-8 and caspase-3 are also involved in non-apoptotic functions. In the development of the yolk sac vasculature during mouse embryogenesis, caspase-8 restricts the RIP3-dependent pathway rather than apoptosis , and caspase-3 is required for the differentiation of embryonic stem cells and hematopoietic stem cells [13, 14].
Another major cellular program mediated by caspases is cytokine processing . In mammals, these caspases are distinctive due to having a caspase recruitment domain (CARD) at the N-terminal. Caspase-1 is the most documented of these. Caspase-1 activity is regulated by the nucleotide olimerization domain-like receptor (NLR) family members through inflammasome formation. These multiprotein complex formations usually require the adaptor apoptosis-associated speck-like protein (ASC), which contains an N-terminal pyrin domain (PYD) interacting with that of the NLR along with a C-terminal CARD with caspase-1. The activated caspase-1 controls maturation of interleukins such as IL-1β and IL-18, which direct a wide variety of effects related to innate immunity and host responses .
Homologs of the three proteins, CED-9, CED-4, and CED-3 (Bcl-2, Apaf-1 and caspase in mammals), which were first identified as being involved in apoptosis in nematodes, are found in the genomes of all animals and associated with apoptosis signaling . It is logical to expect that an apoptotic regulatory network composed of Bcl-2, Apaf-1 and caspase had already been established in protostomes and was conserved to evolve into the intrinsic pathway in deuterostomes. However, cytochrome c binding has not been recognized for CED-4 in Caenorhabditis elegans, and Drosophila Apaf-1 homolog, Dark still remains controversial . Moreover, although a dFADD and a TNFR have been described, such ancient TNFR lacks the death domain (DD) and does not recruit the dFADD , suggesting that the functional extrinsic apoptotic pathway does not exist in Drosophila. In addition, the inflammasome related caspases have been reported only in vertebrates. However, with the annotation of amphioxus immune related genes, an expanded caspase family has been identified in which many genes related to the extrinsic apoptosis pathway, and some novel genes related to inflammation, were identified. Thus, further functional analysis of the amphioxus caspase family will help to clarify whether the caspase-mediated extrinsic apoptosis and the caspase-dependent innate immune complex were completely formed, and what their unique features are, at the basal chordate stage.
Analysis of three caspase genes with death-fold domains in amphioxus
To obtain clues to the functions of distinct caspases, we performed quantitative real-time PCR to detect their tissue distribution (Figure 1B) and responses to infection. The transcripts of all characterized caspases, especially bbtCaspase-1/2, were abundant in the hepatic cecum, gill and gut. Since the hepatic cecum and gut are considered as the first line of the amphioxus immune defense system, we proposed that the caspase family should play important roles in amphioxus immunity. In response to Staphylococcus aureus and Vibrio vulnificus challenges, the transcripts of bbtCaspase-8, but not of bbtCaspase3-like or bbtCaspase-1/2, were upregulated dramatically (Figures 1C and 1D). The distinct expression patterns of caspases may suggest their diverse functions in amphioxus immunity or other roles.
Structure and function conservation of bbtCaspase-8 in apoptosis
A DED-containing nuclear caspase can induce mammalian cell apoptosis
BbtCaspase-1/2 interacts with bbtFADD2 and translocates from the nucleus to the cytoplasm depending on bbtASCs
Additional file 6: Time-lapse video of overlay fluorescences of a HeLa cell co-transfected with bbtCaspase-1/2-GFP and bbtASC-RFP. The video was filmed using a Zeiss microscope for 2 h, and it started filming at 6 h after transfection. (MPEG 624 KB)
Additional file 7: The same video showing only the green channel, bbtCaspase-1/2-GFP. (MPEG 618 KB)
The inhibitors of the extrinsic apoptotic pathway-related caspases block amphioxus embryogenesis in early stages
The novel domain architecture of the expanded caspase family may have led to the emergence of new apoptotic pathways in amphioxus
Genomic annotations of several lower metazoans have revealed an expansion of the caspase family, including 17 homologous genes in Hydra , 31 in the sea urchin  and 45 in amphioxus , compared to only four in the nematode, eight in the fly and twelve in the human. Moreover, compared to those in vertebrates, the death-fold domain (DFD) -containing caspases in amphioxus are expanded by seven- to ten-fold, and include many with novel domain architectures. For example, the single CARD in mammalian caspase-2 is replaced by a tandem DED and DD in bbtCaspase-1/2. Although the bbtCaspase3-like caspase domain is similar to human caspase-3/7, it unexpectedly contains an N-terminal DED. Since all DFDs function as protein-protein interaction facilitators , the domain duplication and shuffling by recombination are probably the main force behind increased or diversified protein functions . Thus, the combining and reshuffling of this domain type in the caspase family provide indications of a greater diversified apoptotic network in amphioxus. This is reflected by the fact that, unlike the reported apoptosis associated with bfCASP-3/7, which has similar domain architecture to human caspase-3/7 , the apoptosis mediated by bbtCaspase3-like is not completely inhibited by the inhibitor z-DEVD-fmk. In addition, bbtCaspase3-like localized in the nucleus independent of its DED, indicating that a previously unreported nuclear cell death pathway may exist in amphioxus, which is distinct from the known caspase-dependent apoptosis.
The conservation of function of bbtCaspase-8 in the extrinsic apoptotic pathway
Although organisms may have evolved distinct apoptosis networks, some core aspects always remained. The extrinsic apoptotic signaling pathway is well known, and was thought to be unique to vertebrates before the publication of sea urchin and amphioxus genomes. Recently, based on ESTs and the whole genome assembly of the ancient metazoan phylum Cnidaria, a DED-containing caspase, a DED-containing FADD-like adaptor protein and a potential death receptor were identified in Hydra . In Drosophila a death adapter, dFADD, which is homologous to the mammalian FADD, was found to bind death-related CED-3-like protein (DREDD) through the death inducing domain (DID), a novel DFD involved in caspase adapter interactions and promoting cell death activity . Our study identified an ortholog of mammalian caspase-8 in Chinese amphioxus, bbtCaspase-8. This gene contains two tandem DEDs, as does its mammalian counterpart, and also interacts with bbtFADD1 through the DEDs to induce mammalian cell death. These results suggest that the core molecules participating in the extrinsic pathway arose early, before the vertebrate, and were conserved in Eumetazoa. In HeLa cells, bbtFADD1 overexpression alone was not found to induce apoptosis , and co-localized with the prodomain of bbtCaspase-8 only, indicating that FADD and the initiator caspase evolved dependently. The FADD proteins are likely specific to cognate initiator caspases, but the proteolytic cascade processes of caspases are generally conserved among species.
The association of bbtCaspase-1/2 with bbtASCs sets up the foundation for vertebrate pro-inflammatory caspases in regulating inflammation
In vertebrates, NLRs usually recruit the adaptor ASC via PYD-PYD interaction. Subsequently, the N-terminal CARD within ASC recruits caspase-1 to form a complex known as an inflammasome . Although five ICE-like genes have been found in the sea urchin genome, none are linked to the CARD prodomain, as is the case in vertebrates, and pro-inflammatory functions of caspases have not been identified in sea urchins. Here, bbtCaspase-1/2 with the highest similarity to mammalian caspase-2 and caspase-1 and two ASC molecules with novel domain architectures were identified in amphioxus. In addition, we demonstrated that bbtASCs interacted with bbtCaspase-1/2 and led bbtCaspase-1/2 to translocate from the nucleus to the cytoplasm. However, no effects on the maturation of IL-1β and IL-18 were observed in different mammalian cells transfected with both bbtCaspase-1/2 and bbtASCs by ELISA testing. One of the possibilities is that the functions of bbtCaspase-1/2 and bbtASCs could not be represented in mammalian cell lines, since the PYD in mammalian ASC was replaced with a DED both in bbtASC-L and bbtASC-S and a DED and DD tandem prodomain architecture in bbtCaspase-1/2 exists only in amphioxus. To date, there are no homologs of human IL-1 and IL-18, which are important products of mammal inflammasomes, found in the amphioxus genome. Moreover, from the analysis of the amphioxus genome, the PYDs of amphioxus NLRs were also replaced by DEDs. Thus, although the interacting basic for the inflammasome-like complex has been established, the mechanism and the effectors in amphioxus should be completely different from those in mammals given their different structures. Here, we also found that bbtCaspase-1/2 co-localizes with bbtFADD2 in the nucleus through the DD, but not with bbtCRADD . In addition, we previously identified that translocation of bbtFADD2 from the nucleus to the cytoplasm induces apoptosis in HeLa cells . Although the underlying mechanism of bbtFADD2 and bbtCaspase-1/2 in amphioxus apoptosis needs to be tested in future successful cultured amphioxus cells, our results still implied that bbtCaspase-1/2 may be involved in an unidentified apoptotic pathway in the nucleus. Thus, bbtCaspase-1/2, which arose from domain recombination, may have emerged as a molecular switch in controlling the balance between caspase-dependent innate immune response and apoptosis in the basal chordate.
Effect of caspase related proteins on amphioxus early embryogenesis
The cell death pathways were originally found in the study of C. elegans and Drosophila development and are essential for normal development of body plan and organ structures. Some knockout gene mouse studies have been conducted in which caspase-1 deficient mice showed no apparent gross abnormalities ; caspase-2 deficient mice were devoid of severe phenotypic abnormalities, with caspase-2 having an impact on neuron apoptosis ; caspase-3 and -7 deficient mice died immediately after birth ; and caspase-8 deficient mouse fetuses did not survive past mid-gestation . As for amphioxus, apoptosis has been examined during amphioxus development and one related protein, bfCASP-3/7 has been proved to be a substrate of caspase inhibitor z-DEVD-fmk in vitro . Apart from the bfCASP-3/7 and the three caspase genes we have identified here, there are at least 40 other caspase related genes. How such expanded caspase related proteins, including the proteins with caspase-like proteolytic activity, like metacaspases [41, 42], are involved in amphioxus development is not known. To address this question, we used several specific caspase inhibitors to treat amphioxus embryos. The embryos treated with caspase-1 inhibitor developed normally. It is possible that no homolog of mammalian caspase-1 exists in the amphioxus genome, so its inhibitor has no target in amphioxus. Alternatively, this protein may have its expression and function in the adult phase, associated with the immune system, because caspase-1 is well-known as an inflammatory caspase in vertebrates. In the sea urchin, ICE-like caspase expression has not been detected in either embryo or larva , and our RT-PCR results indicate that the expression of bbtCaspase-1/2 is much higher than the other two analyzed in adult tissues. Thus, although bbtCaspase-1/2 may interact with bbtFADD2, this potential apoptotic pathway most likely participates in the development of gut openings (mouth and gill slits) and body growth in larvae, but does not influence early embryogenesis, as was seen in the group treated with the caspase-2 inhibitor. Caspase has been reported to show earliest expression in the mesoderm of the gastrula , and bfCASP-3/7 is expressed from gastrula to larva . Overexpression of bfCASP-3/7 induced mammalian cell apoptosis and the inhibitor z-DEVD-fmk blocked its activity . All of these evidences suggested that bfCASP-3/7 is likely to be one of the effector caspase related proteins inhibited by z-DEVD-fmk in embryogenesis. Moreover, bbtCaspase-8 induced HeLa cell apoptosis and such activity can be inhibited by z-IETD-fmk, indicating that z-IETD-fmk may inhibit the activities of caspase-8 related proteins during embryogenesis. In addition, we found that initiator caspases-related proteins may function before effector caspases. Thus, these caspases mediate extrinsic apoptosis, which is indispensable in early embryogenesis, probably by inducing cell differentiation and embryonic layer formation.
Our studies not only indicated that the function of DR-mediated extrinsic apoptosis is conserved and completely formed in amphioxus, but also provided the first evidence of the interacting foundation for the inflammasome-like complex in a non-vertebrate. An interesting point is that many amphioxus caspase genes possess novel domain architectures, which provides important insights into understanding how new signaling pathways emerged along with domain recombination and shuffling, especially at the transition from invertebrates to vertebrates.
Animals and embryos
Adult Chinese amphioxus (Branchiostoma belcheri tsingtauense) were obtained from Kioachow Bay near Qingdao, China. During May and June, ripe males and females, after a 14:10 h light:dark cycle at 22°C for at least seven days, were induced to spawn by thermal shock at 26°C for 36 h. Subsequently, the animals were placed individually in plastic bottles with a small amount of filtered seawater and held in dark. Spawning was checked every hour using red light and the eggs and sperm collected. After in vitro fertilization, isogenous embryos were divided into five groups, four treated with a different caspase inhibitor (Merck, NJ, USA) at 50 μM in DMSO and one with DMSO alone. The embryos were allowed to develop to the desired stage at 23°C.
Sequence retrieval, structural and phylogenetic analyses
The protein sequences of the caspase family were used to perform BLASTp searches against all databases available at the National Center for Biotechnology Information and the DOE Joint Genome Institute. Domain searches were performed against the PROSITE. Protein sequences were first aligned using ClustalX 1.83 and manually corrected using GeneDoc. Then, the neighbor joining tree was obtained using the routine in MEGA 3.1 with 1000 bootstrap tests.
Cloning of bbtCaspase-8, bbtCaspase-1/2, and bbtCaspase3-like cDNAs
Full-length cDNA sequences of three genes were identified in the total cDNA library of Chinese amphioxus. For each, a partial sequence was cloned from Chinese amphioxus cDNA using a specific primer pair derived from B. floridae. Subsequently, 5'-rapid amplification of cDNA ends (RACE) and 3'-RACE were performed according to the manufacturer's protocol of the GeneRacer kit (Invitrogen, USA). After sequencing and manual alignment, we obtained the full-length sequences of bbtCaspase-8, bbtCaspase-1/2, and bbtCaspase3-like. Then, gene specific primers were designed and full-length cDNA sequences of the three amphioxus caspases were obtained from the cDNA library of adult Chinese amphioxus and inserted into T easy vectors for following vectors constructions. Human caspases were cloned following similar procedures with the primers derived from GenBank. BbtCaspase-8, bbtCaspase-1/2 and bbtCaspase3-like sequences have been deposited in the GenBank database [GenBank:JF717867]; [GenBank: JF717868]; [GenBank:JF717869].
Cell lines and expression plasmids
HeLa cells were maintained in DMEM supplemented with 10% fetal calf serum (GIBCO, USA). The ORF and PCR fragments encoding for amino acids 1-193 and 194-606 of bbtCaspase-8; 1-90, 1-226, 91-226, 227-544, 91-544 and 1-90aa+227-544aa of bbtCaspase-1/2; 1-100 and 101-446 of bbtCaspase3-like were cloned by PCR and inserted into vectors pEGFP-N1 and pCMV (Clontech, USA) with a N-terminal HA or FLAG epitope tag, respectively, and designated C8-1, C8-2; C2-1, C2-2, C2-3, C2-4, C2-5, C2-6; C3-1, and C3-2. The ORFs of hsCaspase-3 and hsCaspase-8 were inserted into vectors pEGFP-N1 and pCMV respectively.
Either 105 colony forming units (CFU) of S. aureus or V. vulnificus in PBS were injected into the amphioxus celom and cultured in separate tanks. The challenged animals were collected at 2, 4, 8, 12, 24, 36, 48 and 72 h post-injection. The protocol for the control animals injected with PBS only followed the same schedule. Intestine from five individuals were combined in a single sample for total RNA extraction and subjected to reverse transcription (TOYOBO, Japan). Total RNA from various tissues of un-challenged Chinese amphioxus were extracted and treated with the same way. The 208 bp product of bbtCaspase-8 was amplified by primer pair: 5'-GTCATCGTCAACAACAAAC-3' and 5'-TGGAGTGGTCTTCATAGC-3'; the 113 bp product of bbtCaspase-1/2 was amplified by primer pair: 5'-TTAAGAGCGAGATGAGAAG-3' and 5'-TAGTTGTGTTGCGTATCC-3'; the 186 bp product of bbtCapase3-like was amplified by primer pair: 5'-GGAGATGGAACAGGATGAG-3' and 5'-GAAGACGAGGACGATTGG-3'. All samples were analyzed by RT-PCR in triplicate under the following conditions: 2 min at 95°C followed by 40 cycles of 15 s at 95°C, 15 s at 60°C, and 20 s at 72°C. Data were quantified with the 2-ΔΔCt method based on Ct values of bbtCaspase-8, bbtCaspase-1/2, bbtCaspase3-like and β-actin from two parallel experiments done in triplicate. For expression following challenge, folds were normalized to the expression in PBS-injected animals. Values were considered to be significant at P < 0.05.
HeLa cells were seeded on coverslips (10 mm × 10 mm) in a 24-well plate for more than 12 h and transfected with the indicated expression plasmids using jetPEI (Polyplus Transfection, France) according to the manufacturer's instructions. Twenty to twenty-four hours after transfection, cells were fixed in 4% formaldehyde solution for 15 min. Coverslips were washed with PBST (0.05% Tween-20 in PBS) three times and blocked with 5% BSA in PBST at room temperature for one hour. Primary monoclonal antibodies (Sigma, USA) were added at 1 mg/mL in blocking buffer for 1 hr, and secondary antibodies (1:5000) (Invitrogen, USA) were added for 1 h with three washes between each step. Samples were finally stained with 0.2 μg/mL 4',6-diamidino-2-phenylindole (DAPI) in PBS for 5 min, washed three times in PBS and mounted in MOWIOL R4-88 Reagent (Calbiochem, USA). Fluorescence images were photographed with a Zeiss AxioVision 4 microscope (63 × objective) with appropriate filters.
Annexin V binding apoptosis assay
HeLa cells were cultured in 12-well plates and transfected with 2 μg fused plasmids in pCMV vector for each well. At 36 h post-transfection, cells were trypsinized and collected for analysis with the PE Annexin V Apoptosis Detection Kit I (BD Pharmingen, USA) according to the manufacturer's instructions. The samples were analyzed by a BD FACSCaliburTM cytometer (Becton Dickinson, Heidelberg, Germany). For each sample, 10,000 gated events were required. Annexin V positive cells were considered apoptotic, and the sample transfected empty pCMV vectors were used as a negative control. The positive control was treated with camptothecin (Sigma) at 10 μM for 24 h. Camptothecin was freshly dissolved in DMSO at 10 mM and further diluted in DMEM before each experiment.
Caspase activity assay
HeLa cells were cultured in 48-well plates and transfected with 400 ng/well of indicated plasmids. At 20 h post-transfection, the caspase activity of all samples was measured by Caspase-Glo® 3/7 Assay or Caspase-Glo® 8 Assay (Promega, USA) according to the manufacturer's instructions. The samples transfected empty vectors were used as negative controls and transfected human caspases were positive controls.
HeLa cells in 6-well dishes were transfected with 4 μg of indicated plasmids in each well (2 μg/each expression vector). More than 24 h after transfection, the whole cell extracts were prepared in immunoprecipitation lysis buffer [50 mM Tris, pH 7.4, 150 Mm NaCl, 1% Nonidet P-40, 0.5% deoxycholic acid sodium salt, and cocktail protease inhibitor (Roche, Germany)] and incubated with primary antibodies at 4°C overnight, then incubated with Protein G Sepharose (Roche) at 4°C for 4-6 h. The mix was washed three times with lysis buffer. Analysis was conducted using SDS-PAGE followed by Western blot with enhanced chemiluminescent reagent (Amersham Pharmacia, Finland). The monoclonal antibody against HA epitope tag (1:5000), FLAG epitope tag (1:1000), and the anti-mouse secondary antibody (1:5000) were purchased from Sigma.
apoptosis associated speck-like protein containing a CARD
Branchiostoma belcheri tsingtauense
protein-protein basic local alignment search tool
bovine serum albumin
caspase recruitment domain
CASP2 and RIP1 domain containing adaptor with death domain
death effector domain
Dulbecco's modified Eagle's medium
death-related CED-3-like protein
enzyme-linked immunosorbent assay
expressed sequence tag
Fas-associated death domain
green fluorescent protein
open reading frame
phosphate buffered saline
rapid amplification of cDNA ends
real time polymerase chain reaction
tumor necrosis factor receptor
benzyloxycarbonyl-Val-Asp(OMe)-Val-Ala-Asp(OMe)-fluoromethylketone; z-YVAD-fmk benzyloxycarbonyl-Tyr-Val-Ala-Asp(OMe)-fluoromethylketone.
This work was supported by Project 2007CB815800 and 2011CB946101 of the National Basic Research Program (973), project 2008AA092603 of the State High-Tech Development Project (863), project 2007DFA30840 of International S&T Cooperation Program from the Ministry of Science and Technology of China, key project (0107) from the Ministry of Education, project 30901305 of National Nature Science Foundation of China, and project 11lgzd16 of the Fundamental Research Funds for the Central Universities. Xu is recipient of "Outstanding Young Scientist Award" from National Nature Science Foundation of China.
- Tittel JN, Steller H: A comparison of programmed cell death between species. Genome Biol. 2000, 1: S3-View Article
- Eisenberg-Lerner A, Bialik S, Simon HU, Kimchi A: Life and death partners: apoptosis, autophagy and the cross-talk between them. Cell Death Differ. 2009, 16: 966-975. 10.1038/cdd.2009.33.View ArticlePubMed
- Boyce M, Degterev A, Yuan J: Caspases: an ancient cellular sword of Damocles. Cell Death Differ. 2004, 11: 29-37. 10.1038/sj.cdd.4401339.View ArticlePubMed
- Alnemri ES, Livingston DJ, Nicholson DW, Salvesen G, Thornberry NA, Wong WW, Yuan J: Human ICE/CED-3 protease nomenclature. Cell. 1996, 87: 171-10.1016/S0092-8674(00)81334-3.View ArticlePubMed
- Thornberry NA: Caspases: a decade of death research. Cell Death Differ. 1999, 6: 1023-1027. 10.1038/sj.cdd.4400607.View ArticlePubMed
- Earnshaw WC, Martins LM, Kaufmann SH: Mammalian caspases: structure, activation, substrates, and functions during apoptosis. Annu Rev Biochem. 1999, 68: 383-424. 10.1146/annurev.biochem.68.1.383.View ArticlePubMed
- Nicholson DW: Caspase structure, proteolytic substrates, and function during apoptotic cell death. Cell Death Differ. 1999, 6: 1028-1042. 10.1038/sj.cdd.4400598.View ArticlePubMed
- Denecker G, Hoste E, Gilbert B, Hochepied T, Ovaere P, Lippens S, Van den Broecke C, Van Damme P, D'Herde K, Hachem JP, Borgonie G, Presland RB, Schoonjans L, Libert C, Vandekerckhove J, Gevaert K, Vandenabeele P, Declercq W: Caspase-14 protects against epidermal UVB photodamage and water loss. Nat Cell Biol. 2007, 9: 666-674. 10.1038/ncb1597.View ArticlePubMed
- Riedl SJ, Shi Y: Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol. 2004, 5: 897-907. 10.1038/nrm1496.View ArticlePubMed
- Carrington PE, Sandu C, Wei Y, Hill JM, Morisawa G, Huang T, Gavathiotis E, Wei Y, Werner MH: The structure of FADD and its mode of interaction with procaspase-8. Mol Cell. 2006, 22: 599-610. 10.1016/j.molcel.2006.04.018.View ArticlePubMed
- Li J, Yuan J: Caspases in apoptosis and beyond. Oncogene. 2008, 27: 6194-6206. 10.1038/onc.2008.297.View ArticlePubMed
- Kaiser WJ, Upton JW, Long AB, Livingston-Rosanoff D, Daley-Bauer LP, Hakem R, Caspary T, Mocarski ES: RIP3 mediates the embryonic lethality of caspase-8-deficient mice. Nature. 2011, 471: 368-372. 10.1038/nature09857.PubMed CentralView ArticlePubMed
- Fujita J, Crane AM, Souza MK, Dejosez M, Kyba M, Flavell RA, Thomson JA, Zwaka TP: Caspase activity mediates the differentiation of embryonic stem cells. Cell Stem Cell. 2008, 2: 595-601. 10.1016/j.stem.2008.04.001.PubMed CentralView ArticlePubMed
- Janzen V, Fleming HE, Riedt T, Karlsson G, Riese MJ, Lo CC, Reynolds G, Milne CD, Paige CJ, Karlsson S, Woo M, Scadden DT: Hematopoietic stem cell responsiveness to exogenous signals is limited by caspase-3. Cell Stem Cell. 2008, 2: 584-594. 10.1016/j.stem.2008.03.012.PubMed CentralView ArticlePubMed
- Martinon F, Tschopp J: Inflammatory caspases and inflammasomes: master switches of inflammation. Cell Death Differ. 2007, 14: 10-22. 10.1038/sj.cdd.4402038.View ArticlePubMed
- Schroder K, Tschopp J: The inflammasomes. Cell. 2010, 140: 821-832. 10.1016/j.cell.2010.01.040.View ArticlePubMed
- Zmasek CM, Zhang Q, Ye Y, Godzik A: Surprising complexity of the ancestral apoptosis network. Genome Biol. 2007, 8: R226-10.1186/gb-2007-8-10-r226.PubMed CentralView ArticlePubMed
- Kornbluth S, White K: Apoptosis in Drosophila: neither fish nor fowl (nor man, nor worm). J Cell Sci. 2005, 118: 1779-1787. 10.1242/jcs.02377.View ArticlePubMed
- Kauppila S, Maaty WS, Chen P, Tomar RS, Eby MT, Chapo J, Chew S, Rathore N, Zachariah S, Sinha SK, Abrams JM, Chaudhary PM: Eiger and its receptor, Wengen, comprise a TNF-like system in Drosophila. Oncogene. 2003, 22: 4860-4867. 10.1038/sj.onc.1206715.View ArticlePubMed
- Huang S, Yuan S, Guo L, Yu Y, Li J, Wu T, Liu T, Yang M, Wu K, Liu H, Ge J, Yu Y, Huang H, Dong M, Yu C, Chen S, Xu A: Genomic analysis of the immune gene repertoire of amphioxus reveals extraordinary innate complexity and diversity. Genome Res. 2008, 18: 1112-1126. 10.1101/gr.069674.107.PubMed CentralView ArticlePubMed
- Siegel RM, Martin DA, Zheng L, Ng SY, Bertin J, Cohen J, Lenardo MJ: Death-effector filaments: novel cytoplasmic structures that recruit caspases and trigger apoptosis. J Cell Biol. 1998, 141: 1243-1253. 10.1083/jcb.141.5.1243.PubMed CentralView ArticlePubMed
- Yuan S, Liu H, Gu M, Xu L, Huang S, Ren Z, Xu A: Characterization of the extrinsic apoptotic pathway in the basal chordate amphioxus. Sci Signal. 2010, 3: a66-View Article
- Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger C: A novel assay for apoptosis. Flow cytometric detection of phosphatidylserine expression on early apoptotic cells using fluorescein labelled Annexin V. J Immunol Methods. 1995, 184: 39-51. 10.1016/0022-1759(95)00072-I.View ArticlePubMed
- Carrington PE, Sandu C, Wei Y, Hill JM, Morisawa G, Huang T, Gavathiotis E, Wei Y, Werner MH: The structure of FADD and its mode of interaction with procaspase-8. Mol Cell. 2006, 22: 599-610. 10.1016/j.molcel.2006.04.018.View ArticlePubMed
- Chang HY, Yang X: Proteases for cell suicide: functions and regulation of caspases. Microbiol Mol Biol Rev. 2000, 64: 821-846. 10.1128/MMBR.64.4.821-846.2000.PubMed CentralView ArticlePubMed
- Shearwin-Whyatt LM, Harvey NL, Kumar S: Subcellular localization and CARD-dependent oligomerization of the death adaptor RAIDD. Cell Death Differ. 2000, 7: 155-165. 10.1038/sj.cdd.4400632.View ArticlePubMed
- Vakifahmetoglu-Norberg H, Zhivotovsky B: The unpredictable caspase-2: what can it do?. Trends Cell Biol. 2010, 20: 150-159. 10.1016/j.tcb.2009.12.006.View ArticlePubMed
- Papin S, Cuenin S, Agostini L, Martinon F, Werner S, Beer HD, Grutter C, Grutter M, Tschopp J: The SPRY domain of Pyrin, mutated in familial Mediterranean fever patients, interacts with inflammasome components and inhibits proIL-1beta processing. Cell Death Differ. 2007, 14: 1457-1466. 10.1038/sj.cdd.4402142.View ArticlePubMed
- Bauernfeind F, Ablasser A, Bartok E, Kim S, Schmid-Burgk J, Cavlar T, Hornung V: Inflammasomes: current understanding and open questions. Cell Mol Life Sci. 2010, 68: 765-783.View ArticlePubMed
- Ghayur T, Banerjee S, Hugunin M, Butler D, Herzog L, Carter A, Quintal L, Sekut L, Talanian R, Paskind M, Wong W, Kamen R, Tracey D, Allen H: Caspase-1 processes IFN-gamma-inducing factor and regulates LPS-induced IFN-gamma production. Nature. 1997, 386: 619-623. 10.1038/386619a0.View ArticlePubMed
- Bottger A, Alexandrova O: Programmed cell death in Hydra. Semin Cancer Biol. 2007, 17: 134-146. 10.1016/j.semcancer.2006.11.008.View ArticlePubMed
- Robertson AJ, Croce J, Carbonneau S, Voronina E, Miranda E, McClay DR, Coffman JA: The genomic underpinnings of apoptosis in Strongylocentrotus purpuratus. Dev Biol. 2006, 300: 321-334. 10.1016/j.ydbio.2006.08.053.View ArticlePubMed
- Lasi M, Pauly B, Schmidt N, Cikala M, Stiening B, Kasbauer T, Zenner G, Popp T, Wagner A, Knapp RT, Huber AH, Grunert M, Soding J, David CN, Bottger A: The molecular cell death machinery in the simple cnidarian Hydra includes an expanded caspase family and pro- and anti-apoptotic Bcl-2 proteins. Cell Res. 2010, 20: 812-825. 10.1038/cr.2010.66.View ArticlePubMed
- Park HH, Lo YC, Lin SC, Wang L, Yang JK, Wu H: The death domain superfamily in intracellular signaling of apoptosis and inflammation. Annu Rev Immunol. 2007, 25: 561-586. 10.1146/annurev.immunol.25.022106.141656.PubMed CentralView ArticlePubMed
- Vogel C, Teichmann SA, Pereira-Leal J: The relationship between domain duplication and recombination. J Mol Biol. 2005, 346: 355-365. 10.1016/j.jmb.2004.11.050.View ArticlePubMed
- Bayascas JR, Yuste VJ, Benito E, Garcia-Fernandez J, Comella JX: Isolation of AmphiCASP-3/7, an ancestral caspase from amphioxus (Branchiostoma floridae). Evolutionary considerations for vertebrate caspases. Cell Death Differ. 2002, 9: 1078-1089. 10.1038/sj.cdd.4401075.View ArticlePubMed
- Li P, Allen H, Banerjee S, Franklin S, Herzog L, Johnston C, McDowell J, Paskind M, Rodman L, Salfeld J, Et A: Mice deficient in IL-1 beta-converting enzyme are defective in production of mature IL-1 beta and resistant to endotoxic shock. Cell. 1995, 80: 401-411. 10.1016/0092-8674(95)90490-5.View ArticlePubMed
- Bergeron L, Perez GI, Macdonald G, Shi L, Sun Y, Jurisicova A, Varmuza S, Latham KE, Flaws JA, Salter JC, Hara H, Moskowitz MA, Li E, Greenberg A, Tilly JL, Yuan J: Defects in regulation of apoptosis in caspase-2-deficient mice. Genes Dev. 1998, 12: 1304-1314. 10.1101/gad.12.9.1304.PubMed CentralView ArticlePubMed
- Lakhani SA, Masud A, Kuida K, Porter GJ, Booth CJ, Mehal WZ, Inayat I, Flavell RA: Caspases 3 and 7: key mediators of mitochondrial events of apoptosis. Science. 2006, 311: 847-851. 10.1126/science.1115035.PubMed CentralView ArticlePubMed
- Kang TB, Ben-Moshe T, Varfolomeev EE, Pewzner-Jung Y, Yogev N, Jurewicz A, Waisman A, Brenner O, Haffner R, Gustafsson E, Ramakrishnan P, Lapidot T, Wallach D: Caspase-8 serves both apoptotic and nonapoptotic roles. J Immunol. 2004, 173: 2976-2984.View ArticlePubMed
- Bozhkov PV, Filonova LH, Suarez MF, Helmersson A, Smertenko AP, Zhivotovsky B, von Arnold S: VEIDase is a principal caspase-like activity involved in plant programmed cell death and essential for embryonic pattern formation. Cell Death Differ. 2004, 11: 175-182. 10.1038/sj.cdd.4401330.View ArticlePubMed
- Woltering EJ: Death proteases: alive and kicking. Trends Plant Sci. 2010, 15: 185-188. 10.1016/j.tplants.2010.02.001.View ArticlePubMed
- Holland ND, Holland LZ: Stage- and tissue-specific patterns of cell division in embryonic and larval tissues of amphioxus during normal development. Evol Dev. 2006, 8: 142-149. 10.1111/j.1525-142X.2006.00085.x.View ArticlePubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.