How and why cells grow as rods
© Chang and Huang; licensee BioMed Central 2014
Published: 2 August 2014
The rod is a ubiquitous shape adopted by walled cells from diverse organisms ranging from bacteria to fungi to plants. Although rod-like shapes are found in cells of vastly different sizes and are constructed by diverse mechanisms, the geometric similarities among these shapes across kingdoms suggest that there are common evolutionary advantages, which may result from simple physical principles in combination with chemical and physiological constraints. Here, we review mechanisms of constructing rod-shaped cells and the bases of different biophysical models of morphogenesis, comparing and contrasting model organisms in different kingdoms. We then speculate on possible advantages of the rod shape, and suggest strategies for elucidating the relative importance of each of these advantages.
KeywordsMorphogenesis Cytoskeleton Cell wall
How nanometer-scale molecular components construct micron-scale cells of specific shapes and sizes remains an outstanding question in biology. How are cell shapes generated? Are there reasons why cells have adopted certain shapes over others? Although many shape-determining factors have been identified across divergent organisms, mere characterization of individual cellular components has not revealed how shape is determined, nor has it provided much insight into the context under which these shapes evolved. Addressing these questions will require the integration of biology, physics, and chemistry. For instance, in addition to traditional molecular cell biology, it will be important to understand the role of cellular mechanics, the material properties of cells, their microenvironment, and evolutionary constraints. Comparisons among organisms with a common shape may help to reveal general principles that dictate shape determination and its evolutionary origins.
Here, we explore how cells generate rod-like shapes. The rod, a radially symmetric cylinder with rounded ends, represents a relatively simple geometry that is ubiquitous in unicellular walled organisms. Well-studied examples include bacteria (Escherichia coli, Bacillus subtilis, Agrobacterium tumefaciens), fungi (fission yeast Schizosaccharomyces pombe, Aspergillus nidulens), and plants (pollen tubes, stem and root axis epidermal cells in Arabidopsis thaliana). E. coli and S. pombe even have similar aspect ratios (length is approximately four times width), despite a nearly 100-fold difference in volume and qualitatively different spatial patterns of growth. One speculation is that the shape and aspect ratio of these rods may have particular evolutionary advantages.
Forming a rod
The mechanics of rod-shaped thin shells
Combining equations 1 and 2, these model relationships predict that the circumferential and longitudinal strains (ε r and ε l , respectively) should be linearly dependent on width and turgor pressure and inversely dependent on wall thickness. If Young’s modulus is equal in every direction (mechanically isotropic), then ε r should be twice as large as ε l .
This relationship between the strains in different directions has been used to probe the mechanical properties of the cell wall of rod-shaped cells. In fission yeast, measuring the degree of shrinkage of cells when turgor pressure is reduced reveals this predicted 2:1 strain ratio, suggesting that the cell wall in these cells behaves as an isotropic material (Atilgan and Chang, unpublished observations). In contrast, in rod-shaped bacteria such as E. coli and B. subtilis, cells exhibit a higher degree of longitudinal rather than radial stretching , indicating mechanical anisotropy (or directional dependence), with greater stiffness in the circumferential relative to the longitudinal direction . These observations are consistent with cryo-electron tomograms showing that the E. coli cell wall is organized with the stiffer components (glycan strands) oriented along the circumferential direction . It will be interesting to discover whether there is mechanical anisotropy in plant cell walls, or whether they are more like the fission yeast cell wall.
It is important to note that the anisotropy of growth (elongation along only one axis) can occur using either anisotropic or isotropic wall material; in fact, isotropic material can be used to construct virtually any cell shape. Furthermore, the mechanical properties of the cell wall can be far more complex than the simple scaling relationships we have described above. For example, the relationship between stresses and strains will no longer follow equation 1 at sufficiently large strains; recent atomic force microscopy measurements indicate that the E. coli cell wall exhibits nonlinear properties in its pressurized state that may help the cell resist expansion during hypoosmotic shock . The assumption of a constant thickness across the thin shell may also break down, particularly during septation due to differences in the mode of wall construction at the septum . Ultimately, these mechanical characteristics must be integrated with the patterns of insertion and remodeling of the wall, which can both alter cell-wall thickness and lead to a viscoelastic response in which the wall material flows like a viscous liquid when stressed. This produces a diverse array of potential growth mechanisms in walled cells. Biophysical models can provide testable predictions for the relationships among turgor pressure, growth patterns, and the distribution of strains and growth rate across the cell surface ,.
Growth by cylindrical elongation
In many bacteria, cell growth is achieved by insertion of new cell-wall material at sites throughout the cylindrical part of the cell wall, while insertion is decreased at cell poles. The most well studied organism from the perspective of cell-wall growth is E. coli, with several reviews focusing on the biochemistry , synthesis machinery , morphology ,, and physical characteristics  of the cell wall. Like most bacteria, E. coli has a cell wall composed of peptidoglycan, a macromolecular network of sugar strands (glycans) cross-linked by short peptides. As noted above, the stiffer glycan strands are oriented circumferentially ,, making the cell wall mechanically anisotropic in addition to the growth anisotropy of the rod shape. The cytoskeletal protein MreB, a homolog of eukaryotic actin , moves in an approximately circumferential manner along the inner face of the cytoplasmic membrane, and the cell-wall-targeting antibiotic mecillinam inhibits this motion, suggesting a model in which MreB tracks indicate the paths of insertion of new material on the lateral wall ,. Moreover, E. coli cells twist as they elongate in an MreB-dependent fashion, due to the orientation of glycan strands with a slight angular bias away from the circumferential direction . In B. subtilis, similar coupling of MreB motion to cell-wall synthesis , and twisting (with opposite handedness)  has been observed, suggesting common rules with E. coli for establishing order within the wall despite the difference in wall thickness. It is unknown whether the MreB-guided pattern of cell wall insertion also helps the cell determine and/or maintain its width during growth, though mutations in mreB can result in rods of different sizes .
One predicted consequence of cylindrical elongation is exponential growth, in which single long cells grow faster than short ones. Indeed, E. coli cells elongate exponentially when division is blocked , and appear to do so also during normal growth and division . Exponential growth might be expected of an organism whose growth zone increases proportionally as the cell grows; interestingly, the nature of exponential growth (L = L02t/τ, where τ is the doubling time) dictates that 1/L (dL/dt) = (ln 2)/τ is constant independent of L, indicating that there is no preferred length scale for a given doubling time.
E. coli, along with the curved bacterium Caulobacter crescentus, has been a main subject of theoretical and computational studies of bacterial morphogenesis. Models have fallen into two broad, complementary classes: coarse-grained molecular dynamics simulations of wall mechanics and growth, motivated by hypothesized mechanisms of molecular coordination and/or experimental measurements of cell-wall insertion patterns ,,; and finite-element mechanochemical models that incorporate wall remodeling with mechanical relaxation to predict potential instabilities and scaling relationships among cellular dimensions and growth parameters ,–. A model that considers the balance between the chemical energy released during insertion and change in strain energy due to the new geometry after growth predicts a stable width and growth rate for rod-shaped growth that agrees with measurements of E. coli and B. subtilis for reasonable choices of parameters . Simulations based on this model suggest that MreB exerts an inward force on the cell wall, preventing instabilities in growth due to turgor pressure . Computational models have generally suggested that robust shape determination requires coordination of cell-wall incorporation ,, and molecular-scale simulations suggest that MreB motion may help to maintain cell width along the cell body, particularly during perturbations such as osmotic shock .
Growth by cell-tip extension
In contrast to E. coli, some rod-shaped cells grow via insertion of new cell wall and membrane at the cell tips, while the lateral wall is relatively inert. Mechanisms of tip growth have been investigated in many walled organisms, including S. pombe, hyphal fungi, moss, and pollen tubes, as well as in bacteria such as A. tumefaciens. In general, tip growth is thought to be driven by high turgor pressure that extends the cell wall at the tip, coupled to the addition of new material and the remodeling of old material by a variety of intracellular factors (Figure 1b).
In S. pombe, complex molecular networks have been identified that modulate cell shape, and therefore may be involved at some level in regulating cell wall machinery. Key core cellular processes include exocytosis, endocytosis, actin and microtubule cytoskeletons, and small GTPases such as Rho and Cdc42 (see  for a review). Cdc42 may regulate actin and membrane trafficking to target secretory vesicles containing cell-wall synthases, cell-wall precursors, and membrane to the growth site (Figure 1b) . Although both actin and microtubules are thought to exert forces that push and distort the plasma membrane in animal cells, there is little evidence that they shape walled cells by directly exerting forces . Instead, actin plays at least two critical roles in polarized cell growth: as tracks for myosin-based transport of vesicles to the cell tip, and for endocytosis . Microtubules have a direct role in polarized transport of vesicles in some fungi, such as Aspergillus and Ustilago,. In S. pombe, microtubules play a regulatory role in polarity by depositing Tea proteins that regulate actin and Cdc42 at cell tips , and can direct the formation of a branch under certain circumstances ,. Mathematical models have explored how the Tea proteins act as landmarks to establish gradients of activated Cdc42 ,. Interestingly, Cdc42 activity has been observed to oscillate between the two cell tips with a time scale of about five minutes, which can be modeled using positive and negative feedback loops . It is not known whether the growth of fission yeast varies with these Cdc42 oscillations, although pollen tubes and some hyphal fungi exhibit tip growth in oscillatory pulses . Moreover, some mutants with altered Cdc42 activity exhibit altered cell widths, suggesting a model in which a gradient of Cdc42 activity at cell tips is used to specify the width of the rod ,,. How spatial patterns of polarity factors such as Cdc42 control cell shape through cell wall growth remains poorly understood.
The dimensions of rods
Quantitative measurements of turgor pressure and cell wall properties are also critical for understanding mechanisms of cell-shape determination. Although turgor pressure has been directly measured in large plant cells ,, the smaller sizes of bacteria and yeast have necessitated the development of indirect methods for estimating turgor pressure ,,,. Walled organisms appear to grow under turgor pressures of a few to tens of atmospheres ,. Consistent with the need to bear these turgor stresses, their walls have Young’s moduli of tens to hundreds of MPa (1 atm = 0.1 MPa) ,,, and potentially stiffen under stress . The E. coli cell wall has Young’s modulus of 25 to 100 MPa , and cells experience a turgor pressure of approximately 1 atm ,. Interestingly, B. subtilis cells have turgor pressures roughly 10-fold that of E. coli, and their walls have a similar Young’s modulus but are 10-fold thicker ,, suggesting that perhaps their similar shapes might arise through a common mechanical balance of turgor pressure and wall stresses . Recent estimates of S. pombe cells place Young’s modulus at around 50 MPa and the turgor pressure at 10 to 15 atm  (our unpublished data).
How absolute cell size is specified is unknown in any cell type, and remains one of the outstanding questions in morphogenesis. How do cells specify their dimensions, and how is a certain size (like cell shape) advantageous in evolutionary terms? It is clear that many cells tightly maintain their size as they grow and divide using homeostatic mechanisms ,. For instance, some cells commit to division or DNA replication only after reaching a minimal cell size, suggesting that they have the ability to sense their own size or geometry. S. pombe cells grow to 14 μm in length before entering mitosis and dividing. Recent advances have identified a system of cortical factors including Cdr2 and Pom1 that appear to monitor the surface area of the cell in this process . Similar sizers have been proposed in bacteria . Additional factors affecting cell size are mechanical considerations such as cell wall stress and turgor pressure. For a micron-sized bacterium, an increase in cell width would be coupled to an increase in stress that would entail an increase in stretching of the wall; unless the mechanical properties or thickness of the cell wall were adjusted, a bacterium likely could not expand to reach the size of a S. pombe cell without rupturing. It will be interesting to determine how the mechanical properties and wall thickness vary across closely related species of different sizes, such as Bacillus megaterium (which is approximately 1.5 μm in width) or the larger fission yeast S. japonicus (Figure 3a). Each species may thus attain a certain size that befits its mechanical and growth properties.
Formation of a rod from a sphere
In addition to propagation of shape during growth, cells can face the challenge of initial establishment of shape. Several systems have been established to examine the formation of the rod shape de novo. When S. pombe spores germinate, they generally swell into a nearly spherical shape and then grow a protrusion that eventually extends into a rod of the correct width. Mechanical anisotropy caused by a break in the spore wall and a local accumulation of Cdc42 activity may trigger the initial growth of the protrusion . However, little is known about how the dimensions and shape of the protruding rod are established. Another example of de novo shape formation is in spheroplast regeneration. Upon removal of the cell wall, the resultant S. pombe spheroplast is spherical; when the wall regenerates, a rod of the proper width extends from the larger round cell in the first generation . Bacteria are also able to regenerate into rods. In contrast to yeast, bacterial spheroplasts transition through amorphous shapes to form walled, rod-shaped cells over the course of a few generations ,, and it has recently been shown that this reversion to a rod-shape in B. subtilis can initiate from a completely wall-less state . These behaviors demonstrate that the shape and dimensions of the cell are regulated by robust intracellular mechanisms and do not depend solely on the shape of cells in previous generations.
Maintaining width and keeping the rod straight
A challenge for rod-shaped cells is to maintain cell width during growth. For the rod-shaped bacteria E. coli and B. subtilis, both of which elongate along the cylindrical portion of the cell ,, cell width remains constant even in filamentous cells that grow to lengths approaching 100 microns ,. Similar maintenance of width is seen S. pombe– and in plant pollen tubes. In tip-growing rods, the zone of growth of the tip must remain constant. In bacteria such as E. coli, growth must be coordinated with extension so that width is maintained as cell length increases. Modeling studies have predicted that introducing stress into the new material during incorporation is necessary to prevent turgor-mediated radial expansion ,,; MreB depolymerization causes a gradual increase in cell width , indicating that MreB may play a role in introducing this stress. Critical for testing these models will be the development of genetic and chemical methods for tuning cell width without disrupting overall rod-like shape.
Another challenge for rod-shaped cells is to maintain a linear axis of growth during elongation. How might a cell monitor ‘straightness’? In E. coli cells, the actin-like MreB cytoskeleton localizes preferentially to regions of negative Gaussian curvature, suggesting that MreB polymers sense cell curvature and actively straighten the cell by directing cell-wall insertion to specific sites on the cell surface based on local geometry . In S. pombe, the microtubule cytoskeleton may keep cells straight by coordinating cell-wall growth at the cell tips; microtubules extend across the cell length and transport polarity factors, such as the Tea proteins, to the tips . Mutants with abnormally short microtubules or that lack Tea proteins often grow in a curved shape or sometimes establish an abnormal growth zone on the side of the cell, leading to the formation of a branched, ‘T’-shaped phenotype , suggesting that microtubules contribute to straightness by coordinating the proper zones of growth at the two cell tips ,. Taken together, in both prokaryotes and eukaryotes, the cytoskeleton is at least partially responsible for maintaining cell shape by coordinating local growth patterns with global morphology .
New end formation: a mechanical mechanism
The ends of many rod-shaped cells are roughly hemispherical, with dimensions in accordance with the cylindrical portions of the cell. While the growing end of a tip-growing cell is regulated by many intracellular factors that modulate progressive remodeling of the cell wall, the formation of the new cell end in S. pombe provides an example of how turgor pressure itself can shape the cell wall. During cytokinesis, a cell-wall septum is formed at the division site, guided by the actin-based contractile ring. Afterward, part of the septum is digested away to cause cell separation ; immediately upon separation, the septum changes from a flat shape to the rounded new end. This morphology, which is distinct from the slightly more pointed shape of the growing cell tip, may be produced by a predominantly mechanical mechanism in which turgor pressure inflates the cell wall (our unpublished observations). It will be interesting to see if any Gram-positive bacteria, which have a thicker cell wall than Gram-negatives , and form a septum much as in S. pombe, also shape their new ends in a turgor-mediated manner. By contrast, E. coli cells constrict at mid-cell well before cell separation . This constriction is mediated by the tubulin homolog FtsZ ,, coupled with progressive remodeling of the cell wall to create a hemispherical polar morphology .
Why be a rod?
Given the ubiquity of rod-shaped organisms across kingdoms, it is tempting to speculate that the rod shape represents some kind of geometric optimum. Might there be physical principles and/or evolutionary pressures that favor this morphology? Below, we discuss several speculations on possible benefits of rods relative to other shapes in unicellular microbes, and where possible we suggest general strategies for future experiments that could explicitly test the fitness advantage of a rod relative to other shapes.
Surface area-to-volume ratio
The surface area of a cell influences its ability to communicate with its environment, and impacts critical functions such as respiration and uptake of nutrients. One characteristic of rod-shaped elongation is that the surface area-to-volume ratio remains almost constant as the cell grows, which enables each segment of the cell to experience the same boundary with the environment regardless of length. Relative to a sphere of the same volume, the surface area of a cylinder is increased by approximately 20 to 25% for cells such as E. coli or S. pombe with an aspect ratio of approximately 3 to 4. This increase could be adaptive due to enhanced signaling or transport of nutrients, though a central assumption is that surface transport is limiting for growth.
Another benefit of a rod-like shape is the inherent breaking of symmetry, allowing the cell to concentrate molecules at specific cellular locales. Despite their small size, bacteria are capable of concentrating proteins at different locations in the cell. Several mechanisms have been identified for self-organized localization to the poles of a rod-shaped cell ,, and the proteins at cell poles participate in diverse cellular functions such as chromosome replication and segregation ,, developmental regulation , and motility . For instance, chemotaxis involves the polar localization of chemoreceptor clusters , which has prompted speculation that a rod-like shape optimizes sensing during motility . In tip-growing eukaryotic cells, cell polarization mechanisms allow for the growth machinery, including cell-wall enzymes, membranes, and the cytoskeleton, to be concentrated near the site of cell growth; we speculate that this spatial regulation may allow for optimal rates of growth. Localizing cell wall-modulating enzymes in a small region of the plasma membrane, rather than distributing them over the entire cell surface, may facilitate coordination of the steps in cell-wall remodeling and decrease the frequency of errors in cell-wall synthesis that cause defects in the wall and can subsequently lead to cell lysis.
Efficient cell division
A rod-like shape may also be optimal for efficient and accurate cell division. The longitudinal axis immediately defines a transverse axis for division and may help to specify a well defined mid-plane. In S. pombe, a band of proteins on the plasma membrane specifies the placement of the contractile ring at a site near the medially placed nucleus . Myosin-based forces during construction of the ring may pull components into a structure oriented along the transverse axis by identifying the cross-section of minimal area; in spherical mutants, rings are often mis-positioned and sometimes migrate away from the middle of the cell, leading to errors in cytokinesis . In E. coli and B. subtilis, positioning of the FtsZ-ring is achieved in part by the Min proteins, which localize to the poles and inhibit FtsZ polymerization . The longitudinal axis defines the polarity of the Min-protein gradient and thereby directs FtsZ localization; in round E. coli cells, the Min system has difficulty defining an oscillatory axis .
The rod shape similarly facilitates specification of an axis for segregation of organelles and chromosomes. In S. pombe, the faithful segregation of chromosomes by the mitotic apparatus requires sufficient distance to separate the chromosomes so that the septum does not cut them during cytokinesis . In bacteria, recently discovered spindle-like filament-forming proteins such as ParA/B , grow and align along the long axis of the cell in order to segregate DNA to each end of the cell.
Biofilms and motility
A rod-like shape may also enhance the ability of cells to prosper in their natural environments. The shape of rod-like bacteria has been implicated in swimming and gliding motility ,, which are important components of community organization; a theoretical analysis of the optimum aspect ratio for efficient swimming of a cylindrical body found a value similar to those found in bacteria with flagellar motility such as E. coli.
Shape can also affect cell-cell interactions in communities such as biofilms. A rod-like shape may facilitate the efficient packing of cells , impacting both utilization of nutrients and the mechanical strength of a biofilm. For instance, there is some indication that aberrantly shaped E. coli cells pack less densely on a two-dimensional surface than wild-type cells . In addition to cell shape, numerous other factors contribute to the organization of cells within the colony, including surface interactions and patterns of cellular movements during growth and division; E. coli cells slide past one another after division to pack transversely . In organisms that forage or rely on polarized movement, rod-like shape may be critical to coordinate the movement of a community of cells based on the directionality conferred by the axis of the rods.
Ease of construction
Perhaps the simplest argument for rod-like growth is that only one dimension of the cell changes in time; cell growth only requires extension of the cylindrical cell wall with the same cross-sectional dimensions ,. In contrast with most other shapes (even spherical cells), the rod shape is naturally propagated in the two sister cells after division, with minimal remodeling required. Thus, the rod may be an optimal shape for rapid growth and division, although the nature of this optimality may be difficult to ascertain.
Strategies for testing the fitness advantages of cell shape
How might these speculative advantages of the rod shape be tested? A simple thought experiment would be to vary the shape of a given cell type and test the effects on fitness and various aspects of the cell’s physiology. In some cases, the effects of altering cellular dimensions and shapes have been probed using modeling, providing predictions that can guide future experiments ,. Experimentally, genetic approaches have generated collections of mutants with altered shapes and dimensions in organisms such as E. coli and S. pombe. Quantitative shape analyses of genome-wide mutant libraries promise to identify mutants exhibiting a wide range of shapes and sizes, and comparisons of genome-wide phenotype databases will provide insight to link shape with global cell physiology and fitness.
In addition to mutants with drastically different morphologies, quantitative studies will benefit from mutants with subtle changes in cellular dimensions, providing the opportunity to systematically tune length and width in any study of a given phenotype. For instance, mutations altering a particular residue of MreB confer a range of different cell widths in E. coli (our unpublished observations). In these strains, fitness increased linearly with cell width, providing direct support for the link between cell volume and fitness or growth observed in long-term evolution experiments  and in different nutrient conditions , respectively. Similarly, S. pombe mutants in regulators of the small GTPase Cdc42 display a range of cell widths ,. Physical manipulation of cells using microfabricated devices provides a means to systematically tune cellular dimensions without genetic perturbations ,. Further development of methods for measuring and altering turgor pressure and cell wall properties will complement these approaches.
One challenge facing such studies is the difficulty of disentangling the multiple effects of cell shape, as well as the complexity of the underlying causes of shape alteration. For instance, a mutant defective in cell wall regulation may be wide or round, and exhibit lower fitness because it is prone to lysis, not simply because of its abnormal shape. One way to examine the evolutionary advantages of rod shapes is by making use of evolution itself. Long-term evolution experiments with E. coli have demonstrated that fitter strains are larger in size ,, suggesting that bigger is better, at least under particular laboratory conditions. Deconstructing the molecular origins of both the size changes and fitness advantages in these strains may be informative about the evolutionary pressures that have selected for particular cell sizes.
Further insights will be gained by studying how non-rod-shaped cells evolved. For instance, there are many round bacteria with rapid doubling times . Comparisons of cell growth, chromosome segregation, and cytokinesis mechanisms in these cells versus in rods will likely provide useful insights into advantages of each shape. Similarly, the natural environments of pleiomorphic organisms that shift from a rod to a round shape (or vice versa) may inform our understanding of whether the rod shape is associated with phenotypes such as faster growth or division robustness.
In this review, we have examined mechanisms and possible advantages of rod-shape formation. Consideration of organisms across kingdoms reveals differences and similarities in mechanisms for generating rod-like shapes, and also highlights common advantages that may have driven the convergent evolution of this fundamental shape. We have focused primarily on E. coli and S. pombe as well-characterized examples, but it is likely that studies of other rods will reveal a diverse spectrum of mechanisms of determining cell shape. This review perhaps poses more questions than answers, and sets the stage for future investigations by highlighting, for instance, the question of possible benefits of a given shape in a particular environment.
Our understanding of cell shape-determination mechanisms is still quite rudimentary even in E. coli and S. pombe, and the field would greatly benefit from quantitative interdisciplinary studies investigating morphogenesis at many scales. Many mutants with diverse cell shapes have been identified, and the identification of ways to systematically alter cell shape will facilitate future discoveries. Studies of the rod shape in many organisms should provide the basis for understanding the rationale of other shapes and reveal fundamental principles that specify shape determination in living cells.
Although little is known about the absolute determination of width and length in any organism, detailed studies of both morphology and growth across mutants, related species, and divergent organisms will provide a fingerprint for the underlying physical forces driving size determination. Moreover, genome-scale assays that can profile metabolic activity  and proteomics in a variety of limiting growth environments  will help to connect morphological and physiological phenotypes. While morphogenesis of any living cell is inherently complex, the pinpointing of common threads between organisms will motivate future efforts to address these fundamental questions.
This work was supported by National Institutes of Health R01 GM056836 (to F.C.), and NIH Director’s New Innovator Award DP2OD006466 (to K.C.H.). This work was also supported in part by the National Science Foundation under Grant PHYS-1066293 and the hospitality of the Aspen Center for Physics, and by the Marine Biological Laboratory. The authors thank Rosanna Chau, Alexandre Colavin, and Gabriel Billings for helpful feedback, and Erdinc Atilgan for discussions and for communicating unpublished data.
- Campas O, Mahadevan L: Shape and dynamics of tip-growing cells. Curr Biol. 2009, 19: 2102-2107. 10.1016/j.cub.2009.10.075.PubMedView ArticleGoogle Scholar
- Furchtgott L, Wingreen NS, Huang KC: Mechanisms for maintaining cell shape in rod-shaped Gram-negative bacteria. Mol Microbiol. 2011, 81: 340-353. 10.1111/j.1365-2958.2011.07616.x.PubMed CentralPubMedView ArticleGoogle Scholar
- Bernal R, Rojas ER, Dumais J: The mechanics of tip growth morphogenesis: what we have learned from rubber balloons. J Mech Mater Struct. 2007, 2: 1157-1168. 10.2140/jomms.2007.2.1157.View ArticleGoogle Scholar
- De Pedro MA, Schwarz H, Koch AL: Patchiness of murein insertion into the sidewall of Escherichia coli.Microbiology 2003, 149:1753–1761.,
- Daniel RA, Errington J: Control of cell morphogenesis in bacteria: two distinct ways to make a rod-shaped cell. Cell. 2003, 113: 767-776. 10.1016/S0092-8674(03)00421-5.PubMedView ArticleGoogle Scholar
- Mitchison JM, Nurse P: Growth in cell length in the fission yeastSchizosaccharomyces pombe.J Cell Sci 1985, 75:357–376.,
- Rojas ER, Hotton S, Dumais J: Chemically mediated mechanical expansion of the pollen tube cell wall. Biophys J. 2011, 101: 1844-1853. 10.1016/j.bpj.2011.08.016.PubMed CentralPubMedView ArticleGoogle Scholar
- Brown PJ, de Pedro MA, Kysela DT, Van der Henst C, Kim J, De Bolle X, Fuqua C, Brun YV: Polar growth in the Alphaproteobacterial order Rhizobiales. Proc Natl Acad Sci U S A. 2012, 109: 1697-1701. 10.1073/pnas.1114476109.PubMed CentralPubMedView ArticleGoogle Scholar
- Letek M, Fiuza M, Ordonez E, Villadangos AF, Ramos A, Mateos LM, Gil JA: Cell growth and cell division in the rod-shaped actinomycete Corynebacterium glutamicum.Antonie Van Leeuwenhoek 2008, 94:99–109.,
- Rojas E, Theriot JA, Huang KC: Response of Escherichia coligrowth rate to osmotic shock.Proc Natl Acad Sci U S A 2014, 111:7807–7812.,
- Yao X, Jericho M, Pink D, Beveridge T: Thickness and elasticity of Gram-negative murein sacculi measured by atomic force microscopy. J Bacteriol. 1999, 181: 6865-6875.PubMed CentralPubMedGoogle Scholar
- Gan L, Chen S, Jensen GJ: Molecular organization of Gram-negative peptidoglycan. Proc Natl Acad Sci U S A. 2008, 105: 18953-18957. 10.1073/pnas.0808035105.PubMed CentralPubMedView ArticleGoogle Scholar
- Deng Y, Sun M, Shaevitz JW: Direct measurement of cell wall stress stiffening and turgor pressure in live bacterial cells. Phys Rev Lett. 2011, 107: 158101-10.1103/PhysRevLett.107.158101.PubMedView ArticleGoogle Scholar
- Zhou X, Cegelski L: Nutrient-dependent structural changes in S. aureuspeptidoglycan revealed by solid-state NMR spectroscopy.Biochemistry 2012, 51:8143–8153.,
- Amir A, Nelson DR: Dislocation-mediated growth of bacterial cell walls. Proc Natl Acad Sci U S A. 2012, 109: 9833-9838. 10.1073/pnas.1207105109.PubMed CentralPubMedView ArticleGoogle Scholar
- Dumais J: Modes of deformation of walled cells. J Exp Bot. 2013, 64: 4681-4695. 10.1093/jxb/ert268.PubMedView ArticleGoogle Scholar
- Holtje JV: Growth of the stress-bearing and shape-maintaining murein sacculus of Escherichia coli.Microbiol Mol Biol Rev 1998, 62:181–203.,
- Scheffers DJ, Pinho MG: Bacterial cell wall synthesis: new insights from localization studies. Microbiol Mol Biol Rev. 2005, 69: 585-607. 10.1128/MMBR.69.4.585-607.2005.PubMed CentralPubMedView ArticleGoogle Scholar
- Young KD: The selective value of bacterial shape. Microbiol Mol Biol Rev. 2006, 70: 660-703. 10.1128/MMBR.00001-06.PubMed CentralPubMedView ArticleGoogle Scholar
- Young KD: Bacterial shape: two-dimensional questions and possibilities. Annu Rev Microbiol. 2010, 64: 223-240. 10.1146/annurev.micro.112408.134102.PubMed CentralPubMedView ArticleGoogle Scholar
- Sun SX, Jiang H: Physics of bacterial morphogenesis. Microbiol Mol Biol Rev. 2011, 75: 543-565. 10.1128/MMBR.00006-11.PubMed CentralPubMedView ArticleGoogle Scholar
- Firtel M, Henderson G, Sokolov I: Nanosurgery: observation of peptidoglycan strands in Lactobacillus helveticuscell walls.Ultramicroscopy 2004, 101:105–109.,
- Gitai Z, Dye N, Shapiro L: An actin-like gene can determine cell polarity in bacteria. Proc Natl Acad Sci U S A. 2004, 101: 8643-8648. 10.1073/pnas.0402638101.PubMed CentralPubMedView ArticleGoogle Scholar
- Sv T, Wang S, Furchtgott L, Huang KC, Wingreen NS, Shaevitz JW, Gitai Z: The bacterial actin MreB rotates and rotation depends on cell-wall assembly.Proc Natl Acad Sci U S A 2011, 108:15822–15827.,
- Ursell TS, Nguyen J, Monds RD, Colavin A, Billings G, Ouzounov N, Gitai Z, Shaevitz JW, Huang KC: Rod-like bacterial shape is maintained by feedback between cell curvature and cytoskeletal localization. Proc Natl Acad Sci U S A. 2014, 111: E1025-E1034. 10.1073/pnas.1317174111.PubMed CentralPubMedView ArticleGoogle Scholar
- Wang S, Furchtgott L, Huang KC, Shaevitz JW: Helical insertion of peptidoglycan produces chiral ordering of the bacterial cell wall. Proc Natl Acad Sci U S A. 2012, 109: E595-E604. 10.1073/pnas.1117132109.PubMed CentralPubMedView ArticleGoogle Scholar
- Dominguez-Escobar J, Chastanet A, Crevenna AH, Fromion V, Wedlich-Soldner R, Carballido-Lopez R: Processive movement of MreB-associated cell wall biosynthetic complexes in bacteria. Science. 2011, 333: 225-228. 10.1126/science.1203466.PubMedView ArticleGoogle Scholar
- Garner EC, Bernard R, Wang W, Zhuang X, Rudner DZ, Mitchison T: Coupled, circumferential motions of the cell wall synthesis machinery and MreB filaments in B. subtilis.Science 2011, 333:222–225.,
- Kruse T, Bork-Jensen J, Gerdes K: The morphogenetic MreBCD proteins of Escherichia coliform an essential membrane-bound complex.Mol Microbiol 2005, 55:78–89.,
- Wang P, Robert L, Pelletier J, Dang WL, Taddei F, Wright A, Jun S: Robust growth of Escherichia coli.Curr Biol 2010, 20:1099–1103.,
- Belgrave AM, Wolgemuth CW: Elasticity and biochemistry of growth relate replication rate to cell length and cross-link density in rod-shaped bacteria. Biophys J. 2013, 104: 2607-2611. 10.1016/j.bpj.2013.04.028.PubMed CentralPubMedView ArticleGoogle Scholar
- Misra G, Rojas ER, Gopinathan A, Huang KC: Mechanical consequences of cell-wall turnover in the elongation of a Gram-positive bacterium. Biophys J. 2013, 104: 2342-2352. 10.1016/j.bpj.2013.04.047.PubMed CentralPubMedView ArticleGoogle Scholar
- Jiang H, Si F, Margolin W, Sun SX: Mechanical control of bacterial cell shape. Biophys J. 2011, 101: 327-335. 10.1016/j.bpj.2011.06.005.PubMed CentralPubMedView ArticleGoogle Scholar
- Jiang H, Sun SX: Morphology, growth, and size limit of bacterial cells. Phys Rev Lett. 2010, 105: 028101-10.1103/PhysRevLett.105.028101.PubMed CentralPubMedView ArticleGoogle Scholar
- Kim JS, Sun SX: Morphology of Caulobacter crescentusand the mechanical role of Crescentin.Biophys J 2009, 96:L47–L49.,
- Jiang H, Sun SX: Growth of curved and helical bacterial cells. Soft Matter. 2012, 8: 7446-7451. 10.1039/c2sm25452b.PubMed CentralPubMedView ArticleGoogle Scholar
- Koch AL: The surface stress theory of microbial morphogenesis. Adv Microbial Physiol. 1983, 24: 301-366. 10.1016/S0065-2911(08)60388-4.View ArticleGoogle Scholar
- Drake T, Vavylonis D: Model of fission yeast cell shape driven by membrane-bound growth factors and the cytoskeleton. PLoS Comput Biol. 2013, 9: e1003287-10.1371/journal.pcbi.1003287.PubMed CentralPubMedView ArticleGoogle Scholar
- Goriely A, Tabor M: Biomechanical models of hyphal growth in actinomycetes. J Theoret Biol. 2003, 222: 211-218. 10.1016/S0022-5193(03)00029-8.View ArticleGoogle Scholar
- Goriely A, Tabor M: Self-similar tip growth in filamentary organisms. Phys Rev Lett. 2003, 90: 108101-10.1103/PhysRevLett.90.108101.PubMedView ArticleGoogle Scholar
- Campas O, Rojas E, Dumais J, Mahadevan L: Strategies for cell shape control in tip-growing cells. Am J Bot. 2012, 99: 1577-1582. 10.3732/ajb.1200087.PubMedView ArticleGoogle Scholar
- Cortes JC, Konomi M, Martins IM, Munoz J, Moreno MB, Osumi M, Duran A, Ribas JC: The (1,3)beta-D-glucan synthase subunit Bgs1p is responsible for the fission yeast primary septum formation. Mol Microbiol. 2007, 65: 201-217. 10.1111/j.1365-2958.2007.05784.x.PubMedView ArticleGoogle Scholar
- Ursell TS, Trepagnier EH, Huang KC, Theriot JA: Analysis of surface protein expression reveals the growth pattern of the Gram-negative outer membrane. PLoS Comput Biol. 2012, 8: e1002680-10.1371/journal.pcbi.1002680.PubMed CentralPubMedView ArticleGoogle Scholar
- Chang F, Martin SG: Shaping fission yeast with microtubules. Cold Spring Harb Perspect Biol. 2009, 1: a001347-10.1101/cshperspect.a001347.PubMed CentralPubMedView ArticleGoogle Scholar
- Perez P, Rincon SA: Rho GTPases: regulation of cell polarity and growth in yeasts. Biochem J. 2010, 426: 243-253. 10.1042/BJ20091823.PubMedView ArticleGoogle Scholar
- Minc N, Boudaoud A, Chang F: Mechanical forces of fission yeast growth. Curr Biol. 2009, 19: 1096-1101. 10.1016/j.cub.2009.05.031.PubMed CentralPubMedView ArticleGoogle Scholar
- Schuster M, Treitschke S, Kilaru S, Molloy J, Harmer NJ, Steinberg G: Myosin-5, kinesin-1 and myosin-17 cooperate in secretion of fungal chitin synthase. EMBO J. 2012, 31: 214-227. 10.1038/emboj.2011.361.PubMed CentralPubMedView ArticleGoogle Scholar
- Taheri-Talesh N, Horio T, Araujo-Bazan L, Dou X, Espeso EA, Penalva MA, Osmani SA, Oakley BR: The tip growth apparatus of Aspergillus nidulans.Mol Biol Cell 2008, 19:1439–1449.,
- Minc N, Bratman SV, Basu R, Chang F: Establishing new sites of polarization by microtubules. Curr Biol. 2009, 19: 83-94. 10.1016/j.cub.2008.12.008.PubMed CentralPubMedView ArticleGoogle Scholar
- Terenna CR, Makushok T, Velve-Casquillas G, Baigl D, Chen Y, Bornens M, Paoletti A, Piel M, Tran PT: Physical mechanisms redirecting cell polarity and cell shape in fission yeast. Curr Biol. 2008, 18: 1748-1753. 10.1016/j.cub.2008.09.047.PubMed CentralPubMedView ArticleGoogle Scholar
- Bicho CC, Kelly DA, Snaith HA, Goryachev AB, Sawin KE: A catalytic role for Mod5 in the formation of the Tea1 cell polarity landmark. Curr Biol. 2010, 20: 1752-1757. 10.1016/j.cub.2010.08.035.PubMed CentralPubMedView ArticleGoogle Scholar
- Das M, Drake T, Wiley DJ, Buchwald P, Vavylonis D, Verde F: Oscillatory dynamics of Cdc42 GTPase in the control of polarized growth. Science. 2012, 337: 239-243. 10.1126/science.1218377.PubMed CentralPubMedView ArticleGoogle Scholar
- Kelly FD, Nurse P: Spatial control of Cdc42 activation determines cell width in fission yeast. Mol Biol Cell. 2011, 22: 3801-3811. 10.1091/mbc.E11-01-0057.PubMed CentralPubMedView ArticleGoogle Scholar
- Koch AL: What size should a bacterium be? A question of scale. Annu Rev Microbiol. 1996, 50: 317-348. 10.1146/annurev.micro.50.1.317.PubMedView ArticleGoogle Scholar
- Sliusarenko O, Heinritz J, Emonet T, Jacobs-Wagner C: High-throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio-temporal dynamics. Mol Microbiol. 2011, 80: 612-627. 10.1111/j.1365-2958.2011.07579.x.PubMed CentralPubMedView ArticleGoogle Scholar
- Guberman JM, Fay A, Dworkin J, Wingreen NS, Gitai Z: PSICIC: noise and asymmetry in bacterial division revealed by computational image analysis at sub-pixel resolution. PLoS Comput Biol. 2008, 4: e1000233-10.1371/journal.pcbi.1000233.PubMed CentralPubMedView ArticleGoogle Scholar
- Green PB: Growth physics in Nitella: a method for continuous in vivo analysis of extensibility based on a micro-manometer technique for turgor pressure.Plant Physiol 1968, 43:1169–1184.,
- Milani P, Braybrook SA, Boudaoud A: Shrinking the hammer: micromechanical approaches to morphogenesis. J Exp Bot. 2013, 64: 4651-4662. 10.1093/jxb/ert169.PubMedView ArticleGoogle Scholar
- Tuson HH, Auer GK, Renner LD, Hasebe M, Tropini C, Salick M, Crone WC, Gopinathan A, Huang KC, Weibel DB: Measuring the stiffness of bacterial cells from growth rates in hydrogels of tunable elasticity. Mol Microbiol. 2012, 84: 874-891. 10.1111/j.1365-2958.2012.08063.x.PubMed CentralPubMedView ArticleGoogle Scholar
- Whatmore AM, Reed RH: Determination of turgor pressure in Bacillus subtilis: a possible role for K+ in turgor regulation.J Gen Microbiol 1990, 136:2521–2526.,
- Cayley DS, Guttman HJ, Record MT Jr: Biophysical characterization of changes in amounts and activity of Escherichia colicell and compartment water and turgor pressure in response to osmotic stress. Biophys J 2000, 78:1748–1764.,
- Tuson HH, Renner LD, Weibel DB: Polyacrylamide hydrogels as substrates for studying bacteria. Chem Commun. 2012, 48: 1595-1597. 10.1039/c1cc14705f.View ArticleGoogle Scholar
- Turner JJ, Ewald JC, Skotheim JM: Cell size control in yeast. Curr Biol. 2012, 22: R350-R359. 10.1016/j.cub.2012.02.041.PubMed CentralPubMedView ArticleGoogle Scholar
- Chien AC, Hill NS, Levin PA: Cell size control in bacteria. Curr Biol. 2012, 22: R340-R349. 10.1016/j.cub.2012.02.032.PubMed CentralPubMedView ArticleGoogle Scholar
- Pan KZ, Saunders TE, Flor-Parra I, Howard M, Chang F: Cortical regulation of cell size by a sizer cdr2p. eLife. 2014, 2014: e02040-10.7554/eLife.02040.Google Scholar
- Bonazzi D, Julien JD, Romao M, Seddiki R, Piel M, Boudaoud A, Minc N: Symmetry breaking in spore germination relies on an interplay between polar cap stability and spore wall mechanics. Dev Cell. 2014, 28: 534-546. 10.1016/j.devcel.2014.01.023.PubMedView ArticleGoogle Scholar
- Kelly FD, Nurse P: De novogrowth zone formation from fission yeast spheroplasts.PLoS One 2011, 6:e27977.,
- Lederberg J: Bacterial protoplasts induced by penicillin. Proc Natl Acad Sci U S A. 1956, 42: 574-577. 10.1073/pnas.42.9.574.PubMed CentralPubMedView ArticleGoogle Scholar
- Ranjit DK, Young KD: The Rcs stress response and accessory envelope proteins are required for de novogeneration of cell shape in Escherichia coli.J Bacteriol 2013, 195:2452–2462.,
- Kawai Y, Mercier R, Errington J: Bacterial cell morphogenesis does not require a preexisting template structure. Curr Biol. 2014, 24: 863-867. 10.1016/j.cub.2014.02.053.PubMed CentralPubMedView ArticleGoogle Scholar
- Mitchison JM: The growth of single cells, I. Schizosaccharomyces pombe.Exp Cell Res 1957, 13:244–262.,
- Kubitschek HE, Clay KB: A second growth state for Schizosaccharomyces pombe.Exp Cell Res 1986, 165:243–254.,
- Johnson BF, Miyata M, Miyata H: Morphogenesis of fission yeasts. Molecular Biology of the Fission Yeast. Edited by: Nasim A, Young P, Johnson BF. 1989, Academic Press, New York, 469331-469366.Google Scholar
- Lan G, Wolgemuth CW, Sun SX: Z-ring force and cell shape during division in rod-like bacteria. Proc Natl Acad Sci U S A. 2007, 104: 16110-16115. 10.1073/pnas.0702925104.PubMed CentralPubMedView ArticleGoogle Scholar
- Mata J, Nurse P: Tea1 and the microtubular cytoskeleton are important for generating global spatial order within the fission yeast cell. Cell. 1997, 89: 939-949. 10.1016/S0092-8674(00)80279-2.PubMedView ArticleGoogle Scholar
- Toda T, Umesono K, Hirata A, Yanagida M: Cold-sensitive nuclear division arrest mutants of the fission yeastSchizosaccharomyces pombe.J Mol Biol 1983, 168:251–270.,
- Piel M, Tran PT: Cell shape and cell division in fission yeast. Curr Biol. 2009, 19: R823-R827. 10.1016/j.cub.2009.08.012.PubMed CentralPubMedView ArticleGoogle Scholar
- Theriot JA: Why are bacteria different from eukaryotes?. BMC Biol. 2013, 11: 119-10.1186/1741-7007-11-119.PubMed CentralPubMedView ArticleGoogle Scholar
- Sipiczki M: Splitting of the fission yeast septum. FEMS Yeast Res. 2007, 7: 761-770. 10.1111/j.1567-1364.2007.00266.x.PubMedView ArticleGoogle Scholar
- Matias VR, Beveridge TJ: Cryo-electron microscopy reveals native polymeric cell wall structure in Bacillus subtilis168 and the existence of a periplasmic space.Mol Microbiol 2005, 56:240–251.,
- Reshes G, Vanounou S, Fishov I, Feingold M: Timing the start of division in E. coli: a single-cell study. Phys Biol 2008, 5:046001.,
- Hsin J, Gopinathan A, Huang KC: Nucleotide-dependent conformations of FtsZ dimers and force generation observed through molecular dynamics simulations. Proc Natl Acad Sci U S A. 2012, 109: 9432-9437. 10.1073/pnas.1120761109.PubMed CentralPubMedView ArticleGoogle Scholar
- Li Y, Hsin J, Zhao L, Cheng Y, Shang W, Huang KC, Wang HW, Ye S: FtsZ protofilaments use a hinge-opening mechanism for constrictive force generation.Science 2013, 341:392–395.,
- Huang KC, Mukhopadhyay R, Wingreen NS: A curvature-mediated mechanism for localization of lipids to bacterial poles. PLoS Comput Biol. 2006, 2: e151-10.1371/journal.pcbi.0020151.PubMed CentralPubMedView ArticleGoogle Scholar
- Huang KC, Meir Y, Wingreen NS: Dynamic structures in Escherichia coli: spontaneous formation of MinE rings and MinD polar zones.Proc Natl Acad Sci U S A 2003, 100:12724–12728.,
- Shebelut CW, Guberman JM, van Teeffelen S, Yakhnina AA, Gitai Z: Caulobacterchromosome segregation is an ordered multistep process.Proc Natl Acad Sci U S A 2010, 107:14194–14198.,
- Chen YE, Tropini C, Jonas K, Tsokos CG, Huang KC, Laub MT: Spatial gradient of protein phosphorylation underlies replicative asymmetry in a bacterium. Proc Natl Acad Sci U S A. 2011, 108: 1052-1057. 10.1073/pnas.1015397108.PubMed CentralPubMedView ArticleGoogle Scholar
- Lam H, Matroule JY, Jacobs-Wagner C: The asymmetric spatial distribution of bacterial signal transduction proteins coordinates cell cycle events. Dev Cell. 2003, 5: 149-159. 10.1016/S1534-5807(03)00191-6.PubMedView ArticleGoogle Scholar
- Davis BM, Waldor MK: Establishing polar identity in Gram-negative rods. Curr Opin Microbiol. 2013, 16: 752-759. 10.1016/j.mib.2013.08.006.PubMedView ArticleGoogle Scholar
- Thiem S, Kentner D, Sourjik V: Positioning of chemosensory clusters in E. coliand its relation to cell division.EMBO J 2007, 26:1615–1623.,
- Mishra M, Huang Y, Srivastava P, Srinivasan R, Sevugan M, Shlomovitz R, Gov N, Rao M, Balasubramanian M: Cylindrical cellular geometry ensures fidelity of division site placement in fission yeast. J Cell Sci. 2012, 125: 3850-3857. 10.1242/jcs.103788.PubMedView ArticleGoogle Scholar
- Margolin W: FtsZ and the division of prokaryotic cells and organelles. Nat Rev Mol Cell Biol. 2005, 6: 862-871. 10.1038/nrm1745.PubMedView ArticleGoogle Scholar
- Corbin BD, Yu XC, Margolin W: Exploring intracellular space: function of the Min system in round-shaped Escherichia coli.EMBO J 2002, 21:1998–2008.,
- Lundgren K, Walworth N, Booher R, Dembski M, Kirschner M, Beach D: mik1 and wee1 cooperate in the inhibitory tyrosine phosphorylation of cdc2. Cell. 1991, 64: 1111-1122. 10.1016/0092-8674(91)90266-2.PubMedView ArticleGoogle Scholar
- Ptacin JL, Lee SF, Garner EC, Toro E, Eckart M, Comolli LR, Moerner WE, Shapiro L: A spindle-like apparatus guides bacterial chromosome segregation. Nat Cell Biol. 2010, 12: 791-798. 10.1038/ncb2083.PubMed CentralPubMedView ArticleGoogle Scholar
- Wu Y, Kaiser AD, Jiang Y, Alber MS: Periodic reversal of direction allows Myxobacteria to swarm. Proc Natl Acad Sci U S A. 2009, 106: 1222-1227. 10.1073/pnas.0811662106.PubMed CentralPubMedView ArticleGoogle Scholar
- Cooper S, Denny MW: A conjecture on the relationship of bacterial shape to motility in rod-shaped bacteria. FEMS Microbiol Lett. 1997, 148: 227-231. 10.1111/j.1574-6968.1997.tb10293.x.View ArticleGoogle Scholar
- Kearns DB: A field guide to bacterial swarming motility. Nat Rev Microbiol. 2010, 8: 634-644. 10.1038/nrmicro2405.PubMed CentralPubMedView ArticleGoogle Scholar
- Shapiro JA, Hsu C: Escherichia coliK-12 cell-cell interactions seen by time-lapse video.J Bacteriol 1989, 171:5963–5974.,
- Rudge TJ, Steiner PJ, Phillips A, Haseloff J: Computational modeling of synthetic microbial biofilms. ACS Synthetic Biol. 2012, 1: 345-352. 10.1021/sb300031n.View ArticleGoogle Scholar
- Lenski RE, Travisano M: Dynamics of adaptation and diversification: a 10,000-generation experiment with bacterial populations. Proc Natl Acad Sci U S A. 1994, 91: 6808-6814. 10.1073/pnas.91.15.6808.PubMed CentralPubMedView ArticleGoogle Scholar
- Schaechter M, Maaloe O, Kjeldgaard NO: Dependency on medium and temperature of cell size and chemical composition during balanced grown of Salmonella typhimurium.J Gen Microbiol 1958, 19:592–606.,
- Takeuchi S, DiLuzio WR, Weibel DB, Whitesides GM: Controlling the shape of filamentous cells of Escherichia coli.Nano Lett 2005, 5:1819–1823.,
- Tenaillon O, Rodriguez-Verdugo A, Gaut RL, McDonald P, Bennett AF, Long AD, Gaut BS: The molecular diversity of adaptive convergence. Science. 2012, 335: 457-461. 10.1126/science.1212986.PubMedView ArticleGoogle Scholar
- Domingue G, Costerton JW, Brown MR: Bacterial doubling time modulates the effects of opsonisation and available iron upon interactions betweenStaphylococcus aureusand human neutrophils.FEMS Immunol Med Microbiol 1996, 16:223–228.,
- Bochner BR: Global phenotypic characterization of bacteria. FEMS Microbiol Rev. 2009, 33: 191-205. 10.1111/j.1574-6976.2008.00149.x.PubMed CentralPubMedView ArticleGoogle Scholar
- Ingolia NT, Ghaemmaghami S, Newman JR, Weissman JS: Genome-wide analysis in vivo of translation with nucleotide resolution using ribosome profiling. Science. 2009, 324: 218-223. 10.1126/science.1168978.PubMed CentralPubMedView ArticleGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.