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Figure 5 | BMC Biology

Figure 5

From: TbAGO1, an Argonaute protein required for RNA interference, is involved in mitosis and chromosome segregation in Trypanosoma brucei

Figure 5

TbAGO1-/- cells show delayed growth rate and abnormal cell types. A. Growth curve of wild type (W.T., red squares), TbAGO1-/- (K.O., blue crosses), or of TbAGO1-/- +GFP::TbAGO1 Ticells grown in the absence (-TET, non-induced, open diamonds and dotted green line) or in the presence of tetracycline (+TET, induced, closed diamonds and full green line). B. Analysis of cell types within populations of wild-type (W.T.), TbAGO1-/- (K.O.), or of TbAGO1-/- +GFP::TbAGO1 Ticells grown in the presence of tetracycline (K.O.+GFP::TbAGO1+TET). 1K1N, cells with one kinetoplast and one nucleus; 2K1N, cells with two kinetoplasts and one nucleus; 2K2N, cells with two kinetoplasts and two nuclei; 1K0N, cells with one kinetoplast but without nucleus (zoids) and 1K2N, cells with one kinetoplast and two nuclei. More than 1 000 cells were counted per experiment.

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