Spindle formation is frequently aberrant in TbAGO1-/- cells. Trypanosomes were stained with the anti-tubulin KMX-1 monoclonal antibody and counterstained with DAPI to show nuclear and mitochondrial DNA. Mitotic cells from wild type (WT, top panels) or TbAGO1-/- (KO, central and bottom panels). From left to right, phase contrast image, DAPI staining (blue), tubulin staining (red) and merged DAPI and tubulin staining. The typical elongated mitotic spindle found in wild-type trypanosomes is frequently poorly developed (central panels) or completely bent on one side of the nucleus (bottom panels) in TbAGO1-/- cells.