Figure 3

Separation of kinetoplast and nuclear signal by color deconvolution. Micrographs of cells, as shown in Figure 1, before and after color deconvolution. (A) Procyclic Trypanosoma brucei labeled with 4', 6-diamidino-2-phenylindole (DAPI) (minor groove binding (MGB)) and propidium iodide (PI) (base pair intercalating (BPI)). (B) Promastigote Leishmania mexicana labeled with DAPI (MGB) and SYBR green (BPI). (C) Choanomastigote Crithidia fasciculata labeled with Hoechst 33342 (MGB) and PI (BPI). Scale bar represents 5 μm.