Species | Data source | Ratio of means | Standard deviation |
|---|
| Â | Â | (post-S/pre-S) | Pre-S | Post-S |
|---|
Trypanosoma brucei
| Flow cytometry | 1.83 | 0.08 | 0.10 |
T. brucei
| Manual (nucleus) | 1.62 | 0.11 | 0.16 |
T. brucei
| Auto (nucleus) | 1.90 | 0.14 | 0.24 |
Leishmania mexicana
| Flow cytometry | 2.07 | 0.13 | 0.26 |
L. mexicana
| Manual (nucleus) | 1.85 | 0.26 | 0.41 |
L. mexicana
| Auto (nucleus) | 2.38 | 0.17 | 0.29 |
- Histograms of total DNA content as determined by flow cytometry, nuclear DNA content as measured manually from 4', 6-diamidino-2-phenylindole (DAPI) images (manual) and nuclear DNA content as measured automatically from color-deconvolved images (auto) were fitted to a model of pre-S-phase, post-S-phase and during S-phase changes in DNA content through the cell cycle (Figure 5). The fitting parameters are summarized this table, all values are normalized to the measured pre-S-phase DNA for that data source. The standard deviation required to match the experimental error in the pre-/post-S-phase peaks is a measure of precision. The ratio of the mean of the post-S-phase to the pre-S-phase peak gives a measure of accuracy and is expected to be 2.
