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Table 2 Calibration curves and strains used for the Taq nuclease assay and the TaqMan genotyping assay (SNP).

From: Stability of toxin gene proportion in red-pigmented populations of the cyanobacterium Planktothrix during 29 years of re-oligotrophication of Lake Zürich

TNA Gene locus Strain Calibration curvea E(%)b R 2 c n Quantification limit (cells per template)d Amplicon length (bp)
16S 16S rDNA PCC7821 y = 11.021 - 3.5123x 92.6 0.997 12 4 82
PC-IGS Intergenic spacer of the phycocyanin operon PCC7821 y = 9.9401 - 3.5673x 90.7 0.994 12 4 72
mcyB First adenylation domain of mcyB PCC7821 y = 12.151 - 3.3745x 97.9 0.993 11 4 76
mcyDIS1 3'-end of IS-element within mcyD No.110 y = 7.941 - 3.8823x 81.0 0.994 12 4 93
mcyDIS2 3'-end of IS-element within mcyD No.139 y = 11.956 - 3.1314x 108.6 0.980 11 18 110
mcyAIS 3'-end of IS-element within mcyA No.40 y = 11.126 - 3.6644x 87.5 0.991 11 4 168
mcyHA Deletion between mcyH and mcyA No.62 y = 14.648 - 3.751x 84.8 0.996 12 4 71
PSA I (lineage 1)e Intergenic spacer between psaA and psaB PCC7811 y = 14.144 - 3.4482x 93.2 0.996 11 3 167
PSA II (lineage 2)e Intergenic spacer between psaA and psaB PCC7821 y = 13.681 - 3.3161x 100.2 0.978 9 4 158
SNP Assay allele 1 SNP within mcyT (genotypes that lost the mcy gene cluster) PCC7811 y = 10.449 - 3.6685x 98.2 0.996 15 2 66
SNP Assay allele 2 SNP within mcyT (genotypes containing the mcy gene cluster) PCC7821 y = 15.875 - 3.3579x 98.5 0.996 15 2 66
  1. a Calibration curve = regression line; y = number of PCR cycles at the threshold fluorescent value (Ct), x = amount of template DNA (expressed as log10 of mm3 biovolume per template). b Amplification efficiencies (E) were calculated from E = (10 -1/x-1) × 100 (%), x = slope of calibration curve. c R2 = coefficient of determination of calibration curve. d Quantification limit given as cell number equivalents represents the lower end of the calibration curve. e According to [4].