TNA | Gene locus | Strain | Calibration curvea | E(%)b | R 2 c | n | Quantification limit (cells per template)d | Amplicon length (bp) |
---|---|---|---|---|---|---|---|---|
16S | 16S rDNA | PCC7821 | y = 11.021 - 3.5123x | 92.6 | 0.997 | 12 | 4 | 82 |
PC-IGS | Intergenic spacer of the phycocyanin operon | PCC7821 | y = 9.9401 - 3.5673x | 90.7 | 0.994 | 12 | 4 | 72 |
mcyB | First adenylation domain of mcyB | PCC7821 | y = 12.151 - 3.3745x | 97.9 | 0.993 | 11 | 4 | 76 |
mcyDIS1 | 3'-end of IS-element within mcyD | No.110 | y = 7.941 - 3.8823x | 81.0 | 0.994 | 12 | 4 | 93 |
mcyDIS2 | 3'-end of IS-element within mcyD | No.139 | y = 11.956 - 3.1314x | 108.6 | 0.980 | 11 | 18 | 110 |
mcyAIS | 3'-end of IS-element within mcyA | No.40 | y = 11.126 - 3.6644x | 87.5 | 0.991 | 11 | 4 | 168 |
mcyHA | Deletion between mcyH and mcyA | No.62 | y = 14.648 - 3.751x | 84.8 | 0.996 | 12 | 4 | 71 |
PSA I (lineage 1)e | Intergenic spacer between psaA and psaB | PCC7811 | y = 14.144 - 3.4482x | 93.2 | 0.996 | 11 | 3 | 167 |
PSA II (lineage 2)e | Intergenic spacer between psaA and psaB | PCC7821 | y = 13.681 - 3.3161x | 100.2 | 0.978 | 9 | 4 | 158 |
SNP Assay allele 1 | SNP within mcyT (genotypes that lost the mcy gene cluster) | PCC7811 | y = 10.449 - 3.6685x | 98.2 | 0.996 | 15 | 2 | 66 |
SNP Assay allele 2 | SNP within mcyT (genotypes containing the mcy gene cluster) | PCC7821 | y = 15.875 - 3.3579x | 98.5 | 0.996 | 15 | 2 | 66 |