Transcription factors activated by recombinant protein secretion. Transcription factors were scored based on significantly changed genes in (a) wild-type strains, and (b) Δhac1. Venn diagram shows the number of secretory-related transcription factors activated in insulin precursor and α-amylase compared to no protein secretion. Table lists secretory-related transcription factors in small (insulin precursor) and large (α-amylase) protein secretion. Color coding indicates common secretory mechanisms as shown in diagram: modifying trans-Golgi network sorting (orange), oxidative stress (purple), amino acid metabolism (blue), and transcription and translation (green).