Figure 4

Comparison of HHARI RING2 with Parkin RING2. (a) Multiple sequence alignment of HHARI RING2 and Parkin RING2. Zn²⁺-liganding residues determined structurally for HHARI RING2 are denoted in yellow. Potential additional Zn²⁺ coordinating residues in Parkin as proposed by Rankin et al. [34] are highlighted in red. The active site cysteine is denoted by a double dagger. (b) The structure of HHARI RING2 displays Zn²⁺-liganding residues as yellow sticks. The active site cysteine is shown as orange sticks (PDB ID 1WD2). Sequences were aligned using CLUSTALW [44].