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Table 1 Tumor cell lines

From: Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation

Cell line

Tumor type

Three-dimensional morphology

Supplier

Medium

U-87 MG

GBM (adult)

T

ATCC

1

A172

GBM (adult)

T

ATCC

1

SF126

GBM (adult)

T

JCRB

1

LN-229

GBM (adult)

T

ATCC

1

SF268

GBM (adult)

C

NCI repository

1

SF188

GBM (pediatric)

T

Bax, 2009 [56]

2

KNS42

GBM (pediatric)

T

Bax, 2009 [56]

2

RES186

Astrocytoma (pediatric)

T

Bax, 2009 [56]

2

RES259

Astrocytoma (pediatric)

T

Bax, 2009 [56]

2

UW479

Astrocytoma (pediatric)

L

Bax, 2009 [56]

2

WT-CLS1

Wilm's kidney

T

CRUK Cell Line Services

1

WiT49

Wilm's kidney

T

Dr H Yeger, Hospital for Sick Children, Toronto

1

IMR-32

Neuroblastoma

T

ATCC

1

SH-SY5Y

Neuroblastoma

C

Dr L Chesler, ICR

3

KELLY

Neuroblastoma

C

Dr L Chesler, ICR

3

SHEP

Neuroblastoma

T

Dr L Chesler, ICR

3

U-2 0S

Osteosarcoma

C

ATCC

1

Detroit 562

SCC (pharynx)

T

ATCC

1

CAL 27

SCC (tongue)

T

ATCC

1

PECA/CA-PJ34

SCC (basaloid)

T

ECACC

4

PE/CA-PJ41a

SCC (oral)

C

ECACC

4

SIHN-011A

SCC (tongue)

C

O-charoenrat, 2001 [57]

1

LICR-LON-HN4

SCC (larynx)

T

Easty, 1981 [58]

1

SK-Mel-5

Melanoma

L

ATCC

1

NCI-H23

Lung

C

ATCC

3

MDA-MB-231a

Breast

C

ATCC

1

MCF-7

Breast

C

ATCC

1

DLD-1

Colon

T

ATCC

1

HCT 116

Colon

T

ATCC

1

RKOa

Colon

L

ATCC

1

HT-29

Colon

T

ATCC

1

SW620a

Colon

L

ATCC

1

PC-3a

Prostate

L

ATCC

3

A2780

Ovarian

L

ECACC

3

IGROV-1a

Ovarian

L

CRUK Cell Line Services

3

SK-OV-3a

Ovarian

L

ATCC

3

PANC-1a

Pancreatic

C

ATCC

1

MIA PaCa-2

Pancreatic

L

ATCC

1

Hep G2

Hepatocellular

C

ATCC

5

Hep 3B

Hepatocellular

C

ATCC

5

  1. aCell lines in which three-dimensional structure is improved by addition of Matrigel at spheroid initiation.
  2. Medium: 1 = DMEM; 2 = DMEM:F12; 3 = RPMI; 4 = Iscove's Modified Dulbecco's Medium; 5 = Eagle's Minimum Essential Medium.
  3. ATCC = American Type Culture Collection; C = compact aggregate; ECACC = European Collection of Cell Cultures; GBM = glioblastoma; JCRB = Japanese Collection of Research Bioresources; L = loose aggregate; SCC = squamous cell carcinoma; T = tight spheroid