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Table 1 Tumor cell lines

From: Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation

Cell line Tumor type Three-dimensional morphology Supplier Medium
U-87 MG GBM (adult) T ATCC 1
A172 GBM (adult) T ATCC 1
SF126 GBM (adult) T JCRB 1
LN-229 GBM (adult) T ATCC 1
SF268 GBM (adult) C NCI repository 1
SF188 GBM (pediatric) T Bax, 2009 [56] 2
KNS42 GBM (pediatric) T Bax, 2009 [56] 2
RES186 Astrocytoma (pediatric) T Bax, 2009 [56] 2
RES259 Astrocytoma (pediatric) T Bax, 2009 [56] 2
UW479 Astrocytoma (pediatric) L Bax, 2009 [56] 2
WT-CLS1 Wilm's kidney T CRUK Cell Line Services 1
WiT49 Wilm's kidney T Dr H Yeger, Hospital for Sick Children, Toronto 1
IMR-32 Neuroblastoma T ATCC 1
SH-SY5Y Neuroblastoma C Dr L Chesler, ICR 3
KELLY Neuroblastoma C Dr L Chesler, ICR 3
SHEP Neuroblastoma T Dr L Chesler, ICR 3
U-2 0S Osteosarcoma C ATCC 1
Detroit 562 SCC (pharynx) T ATCC 1
CAL 27 SCC (tongue) T ATCC 1
PECA/CA-PJ34 SCC (basaloid) T ECACC 4
PE/CA-PJ41a SCC (oral) C ECACC 4
SIHN-011A SCC (tongue) C O-charoenrat, 2001 [57] 1
LICR-LON-HN4 SCC (larynx) T Easty, 1981 [58] 1
SK-Mel-5 Melanoma L ATCC 1
NCI-H23 Lung C ATCC 3
MDA-MB-231a Breast C ATCC 1
MCF-7 Breast C ATCC 1
DLD-1 Colon T ATCC 1
HCT 116 Colon T ATCC 1
RKOa Colon L ATCC 1
HT-29 Colon T ATCC 1
SW620a Colon L ATCC 1
PC-3a Prostate L ATCC 3
A2780 Ovarian L ECACC 3
IGROV-1a Ovarian L CRUK Cell Line Services 3
SK-OV-3a Ovarian L ATCC 3
PANC-1a Pancreatic C ATCC 1
MIA PaCa-2 Pancreatic L ATCC 1
Hep G2 Hepatocellular C ATCC 5
Hep 3B Hepatocellular C ATCC 5
  1. aCell lines in which three-dimensional structure is improved by addition of Matrigel at spheroid initiation.
  2. Medium: 1 = DMEM; 2 = DMEM:F12; 3 = RPMI; 4 = Iscove's Modified Dulbecco's Medium; 5 = Eagle's Minimum Essential Medium.
  3. ATCC = American Type Culture Collection; C = compact aggregate; ECACC = European Collection of Cell Cultures; GBM = glioblastoma; JCRB = Japanese Collection of Research Bioresources; L = loose aggregate; SCC = squamous cell carcinoma; T = tight spheroid