From: A never ending race for new and improved fluorescent proteins
FP property | Examples for relevance | Reference |
---|---|---|
Reduce or increase sensitivity to environmental changes (for example, pH, ionic conditions) | Reduce sensitivity to gain specificity of response as in metabolite sensors; or increase as in high pH sensitivity of Keima tracer for compartmentalization and membrane topology | [a] |
Monomer versus oligomerization | Especially important for environments in which diffusion is constrained like membranes | [b-e] |
Folding time | Important for time-sensitive expression studies and some cell biology applications like protein trafficking | [f] |
Brightness (quantum yield and extinction coefficient) | Generally, brighter FPs are important for getting signal above high background or when imaging biomolecules or processes that have low abundance (for example, when measuring expression from weak promoters) | [g-i] |
Absorption and emission spectra (color change, stokes shift, narrowing of spectra) | Important for avoiding autofluorescence, multicolor imaging and/or specialized imaging modalities such as multiphoton and Stimulated emission depletion microscopy (STED) | [a, j, k-p] |
Monoexponential decay | Important for Fluorescence-lifetime imaging microscopy (FLIM) | [g, q] |
Photoswitching, photoactivation and photoconversion | Important for monitoring dynamics of proteins and protein populations | [r, s] |
Photostability | Important for extended, high-resolution and/or single molecule imaging | [e, t, u] |