Figure 5

ML species tree obtained from the concatenated alignment of 53 widespread, single-copy proteins (Additional file 1, Table S6) extended with newly available three microsporidian species and one zygomycotina species (Additional file 1, Table S5). The alignment was then trimmed to remove non-informative columns and columns that contained only gaps for the nine microsporidian species considered. The ML tree was reconstructed using LG as evolutionary model and SPR as tree topology search method as recommended in PhyML. A discrete gamma-distribution with four rate categories plus invariant positions was used, estimating the gamma parameter and the fraction of invariant positions from the data. Branch supports are SH-based aLRT statistics. Nodes with support below 1.0 are marked on the tree.