Figure 2

Transcriptional responses of yeast exposed to gene mistranslations and environmental stressors. A) Gene expression profiles of mistranslating cells at T0', T40', T60', T90', T120' and T180'. B) Overlap of genes differentially expressed (DEGs) in the Environmental Stress Response (ESR) and in mistranslations (> 2-fold deregulation). Approximately 70% of the mistranslations DEGs are related to the stress response. The overlap of genes up-regulated by the ESR and mistranslations increased over time reaching 82% at mistranslation T180'. Similarly, the overlap of down-regulated genes increases significantly at mistranslations T120' and T180'. C) Summary of GO terms of ESR and mistranslations DEGs. Each color square represents the average expression level of the genes annotated with the corresponding GO term for each stress condition. Stress conditions have been hierarchically clustered. Mistranslations activate stress responders and repress translational and ribosomal biogenesis processes, although average fold variation is not as strong as for heat shock or nitrogen depletion. The ESR up- and down-regulated gene lists (ESRup, ESRdown) were obtained from Gasch et al. [42].