buffy mutants maintain lower ATP and higher lactate concentrations than wild-type larvae. (A) ATP concentration in fed buffy mutant larvae is lower than wild-type. ATP concentration is expressed relative to wild-type fed. Mean ± SEM (n = 3 for fed and protein-starved experiments, n = 2 for water-starved experiment). Fed buffy ATP concentration was 48%, 82% and 83% of wild-type in the biological replicates. (B) Fed larvae lacking buffy have a higher concentration of lactate compared to wild-type larvae. Lactate concentration was determined for lysates from pools of 15 larvae. Mean ± SEM (wild-type fed, n = 5; buffyH37 fed, n = 5; wild-type starved, n = 3; buffyH37 starved, n = 4).