Figure 2From: Drosophila larvae lacking the bcl-2 gene, buffy, are sensitive to nutrient stress, maintain increased basal target of rapamycin (Tor) signaling and exhibit characteristics of altered basal energy metabolismbuffy mutants maintain lower ATP and higher lactate concentrations than wild-type larvae. (A) ATP concentration in fed buffy mutant larvae is lower than wild-type. ATP concentration is expressed relative to wild-type fed. Mean ± SEM (n = 3 for fed and protein-starved experiments, n = 2 for water-starved experiment). Fed buffy ATP concentration was 48%, 82% and 83% of wild-type in the biological replicates. (B) Fed larvae lacking buffy have a higher concentration of lactate compared to wild-type larvae. Lactate concentration was determined for lysates from pools of 15 larvae. Mean ± SEM (wild-type fed, n = 5; buffyH37 fed, n = 5; wild-type starved, n = 3; buffyH37 starved, n = 4).Back to article page