Effects of SMX on C. elegans folates and folate-dependent functions. A) SMX causes a dose-dependent decrease in C. elegans 5-methylTHFGlu5 levels. Data shown are from three biological replicates. B) Methotrexate induces slowed development, sterility and uncoordination in nuc-1 mutant worms but 128 μg/ml SMX has no effect. Pictures were taken three days after egg-laying. C) 16 and 128 μg/ml SMX have no effect on development time, egg-laying schedule or total brood size of N2 worms raised at 25°C. Control (number of animals = 15), 16 μg/ml SMX (n = 18), 128 μg/ml SMX (n = 17). Error bars are ± standard deviation.