Figure 1

Rho inhibition modulates peripheral fascin-containing protrusions. (A) C2C12 cells (control or treated with the indicated pharmacological inhibitors), were plated onto 50 nmol/l fibronectin (FN) for 1 hour, then fixed and stained for fascin-1. Arrowheads indicate examples of fascin-containing protrusions, dotted arrow indicates fascin in association with stress fibers. Boxed areas are enlarged below. Scale bars, 10 μm. (B) Representative results of rhotekin-Rho-binding domain (RBD) pull-down of Rho-guanine triphosphate (GTP) from C2C12 cells adherent on 30 nmol/l FN or thrombospondin-1 for 1 hour, or suspended for 90 minutes over BSA-coated plastic. (C,D) Quantification of (C) numbers and (D) length of peripheral fascin bundles in C2C12 cells adherent for 1 hour on 50 nnmol/l FN after each treatment. Each column represents the mean from 70 to 100 cells from 3 independent experiments; bars indicate SEM. *P < 0.001 versus control. (E) SW480 cells (control or treated with the indicated pharmacological inhibitors), were plated onto 15 nnmol/l laminin (LN) for 2 hours, then fixed and stained for fascin-1. Arrowheads indicate examples of fascin-containing protrusions; boxed areas are enlarged below. Scale bars, 10 μm.