Table 1 Primer and oligonucleotide pairs used for PCR, cloning and site-directed mutagenesis
Name | Direction | Sequence |
|---|---|---|
fascin-1S39A/D cDNAs | Forward | GTACGGATCCATGACCGCCAACGGCACAGCC |
Subclonings into pcDNA3.mRFP(N) | Reverse | GTACGAATTCCTAGTACTCCCAGAGCGAGGC |
fascin-1 cDNA | Forward | CCGGGAAGCTTCGCAGCGGCCTCTCG |
Subcloning into pCR-Blunt | Reverse | GACGGGCCTAGGCTAGTACTCCCAGAG |
LIMK1/460A mutagenesis | Forward | GAACATCATCCACCGAGCCCTCAACTCCCACAAC |
| Â | Reverse | GTTGTGGGAGTTGAGGGCTCGGTGGATGATGTTC |
LIMK1/508A mutagenesis | Forward | GCAAGAAGCGCTACGCCGTGGTGGGCAAC |
| Â | Reverse | GTTGCCCACCACGGCGTAGCGCTTCTTGC |
LIMK2/451A mutagenesis | Forward | GTGCATCATCCACCGGGCTCTGAACTCGCACAACTG |
| Â | Reverse | CAGTTGTGCGAGTTCAGAGCCCGGTGGATGATGCAC |
LIMK2/505A mutagenesis | Forward | CGCAAGAAGCGCTACGCGGTGGTGGGAAACC |
| Â | Reverse | GGTTTCCCACCACCGCGTAGCGCTTCTTGCG |