Microarray analysis of seedlings ectopically expressing FUS3. (A) Images showing the development of leaf 4 in 5-day-old fus3 ML1:FUS3-GR seedlings transferred to different concentrations of dexamethasone (DEX). (B) Genes that change at least twofold in expression in fus3 ML1:FUS3-GR seedlings treated with 1 μM DEX. The upper graph (2 days ± DEX) shows the ratios of fold changes in gene expression of 5-day-old seedlings grown for 2 days in the absence of DEX (black bars) or in the presence of DEX (red bars represent upregulated genes, and blue bars represent downregulated genes). The same order of genes is represented in the lower graph (4 days ± DEX), where 5-day-old seedlings were grown for 4 days in the absence of DEX (black bars) or in the presence of DEX (red and blue bars). (C) The proportions of genes associated with various molecular functions (gene ontology) are represented in the pie chart. (D) RT-PCR verification of ethylene-related genes identified by microarray analysis downregulated by FUS3 activation. Expression of four ethylene-related genes and an ACTIN7 (ACT7) control in the absence (-DEX) or presence (+DEX) of FUS3 activation.