Table 1 Primers used in this study
From: Rapid production of antigen-specific monoclonal antibodies from a variety of animals
Name | Sequence | Application |
|---|---|---|
human IgGV AS1 | ACGCTGCTGAGGGAGTAGAGTCCTGAG | 5' RACE PCR human VH first |
human IgGV AS2 | AGCCGGGAAGGTGTGCACGCCGCTG | 5' RACE PCR human VH second |
human IgKV AS1 | CTTTGGCCTCTCTGGGATAGAAGTT | 5' RACE PCR human VLκ first |
human IgKV AS2 | ACAACAGAGGCAGTTCCAGATTTCAACTGC | 5' RACE PCR human VLκ second |
human IgλV AS1 | TCACDGSYCCCGGGTAGAAGTCACT | 5' RACE PCR human VLλ first |
human IgλV AS2 | AGTGTGGCCTTGTTGGCTTG | 5' RACE PCR human VLλ second |
rat IgGC S | AGAGACTCGAGTGGAACTCTGGAGCCCTGTCCAGCG | Rat IgG constant gene cloning |
rat IgGC AS | CTCTCTGCGGCCGCGGGTCATTTACCCGGAGAGTGGGAGAG | Rat IgG constant gene cloning |
rat IgKC S | AGAGACTCGAGGGGCTGATGCTGCACCAACTGTATC | Rat IgK constant gene cloning |
rat IgKC AS | CTCTCTGCGGCCGCGTCTAACACTCATTCCTGTTGAAGCTC | Rat IgK constant gene cloning |
rat IgGV AS1 | GCAGGTGACGGTCTGGCTGGRCCAGGTGCTGGA | 5' RACE PCR rat VH first PCR |
rat IgGV AS2 | CTGCAGGACAGCTGGGAAGGTGTGCAC | 5' RACE PCR rat VH second PCR |
rat IgKV AS1 | TAACTGTTCCGTGGATGGTGGGAAGAT | 5' RACE PCR rat VLκ first PCR |
rat IgKV AS2 | TCGTTCAGTGCCATCAATCTTCCACTTGAC | 5' RACE PCR rat VLκ second PCR |
rat IgH cassette S | TGGAACTCTGGAGCCCTGTCCAGCGGTG | Rat IgH cassette amplification |
rat IgLk cassette S | GGGCTGATGCTGCACCAACTGTATC | Rat IgLk cassette amplification |
rab IgGC S | AGAGAGCTCGAGTGCCTGGTCAAAGGCTACCTCCCG | Rabbit IgG constant gene cloning |
rab IgGC AS | GTGTGTGCGGCCGCGCTCATTTACCCGGAGAGCGGGAGAT | Rabbit IgG constant gene cloning |
rab IgKC S | AGAGAGCTCGAGGATCCAGTTGCACCTACTGTCCTCA | Rabbit IgK constant gene cloning |
rab IgKC AS | AGAGAGGCGGCCGCTCTAGCAGTCACCCCTGTTGAAGCT | Rabbit IgK constant gene cloning |
rab IgGV AS1 | GCTGGCTGCTTGAGGTCACGCTCACCAC | 5' RACE PCR rabbit VH first PCR |
rab IgGV AS2 | CTGCCGGACGGACGGGAAGGTGCGTAC | 5' RACE PCR rabbit VH second PCR |
rab IgKV AS1 | CAGTTGTTTGGGTGGTGCCATCCAC | 5' RACE PCR rabbit VLκ first PCR |
rab IgKV AS2 | GGGTGGTGCCATCCACCTCCCAGGTGAC | 5' RACE PCR rabbit VLκ second PCR |
rab IgH cassette S | CCAGTTGCACCTACTGTCCTCATCTTCC | Rabbit IgH cassette amplification |
rab IgLk cassette S | GATCCAGTTGCACCTWCTGTCCTCMTCTTCC | Rabbit IgLk cassette amplification |
g-pig IgGC S | AGAGACTCGAGTGCCTGGTCAAGGGCTACTTCCCTGA | Guinea pig IgG constant gene cloning |
g-pig IgGC AS2 | ATCTCCCGGTCTCCGGGTAAATGAGCGGCCGCTCTCTC | Guinea pig IgG constant gene cloning |
g-pig IgKC S | AGAGACTCGAGGGGACCAAGCTGGAAATCAAACGGA | Guinea pig IgK constant gene cloning |
g-pig IgKC AS | TATATAGCGGCCGCCTAGCACTCGCTCCTGTTGATGGTCT | Guinea pig IgK constant gene cloning |
g-pig IgλC S | AGAGACTCGAGTGCCTGGTCAAGGGCTACTTCCCTGA | Guinea pig Igλ constant gene cloning |
g-pig IgλC AS | GAGAGAGCGGCCGCTCATTTACCCGGAGACCGGGAGAT | Guinea pig Igλ constant gene cloning |
g-pig IgGV AS1 | CTTGTCCACCTTGGTGCTGCTGGCCGGGTG | 5' RACE PCR guinea pig VH first |
g-pig IgGV AS2 | GACTGAAGGACGGCCGGGAAGGTGTGCAC | 5' RACE PCR guinea pig VH second |
g-pig IgKV AS1 | CAGAGCCATCCACCTTCCACTTGACGG | 5' RACE PCR guinea pig VLκ first |
g-pig IgKV AS2 | GAAGAGGGAGATAGTTGGCTTCTGCACACT | 5' RACE PCR guinea pig VLκ second |
g-pig IgλV AS1 | CTGCTGGCCATGTATTTGTTGTCGCTCTG | 5' RACE PCR guinea pig VLλ first |
g-pig IgλV AS2 | AGAAGGAATTCAGGAGACACACCACTGT | 5' RACE PCR guinea pig VLλ second |
g-pig IgH cassette S | TGCCTGGTCAAGGGCTACTTCCCTGAGC | Guinea pig IgH cassette amplification |
g-pig IgLk cassette S | GGGACCAAGCTGGAAATCAAACGGAG | Guinea pig IgLk cassette amplification |
g-pig IgLλ cassette S | GAGGAGCTCCAGGACAACAAGGCCAC | Guinea pig IgLλ cassette amplification |
Nhe polyC S | GCTAGCGCTACCGGACTCAGATCCCCCCCCCCCCCDN | 5' RACE PCR first |
Nhe-Eco47 | CGCTAGCGCTACCGGACTCAGATCCC | 5' RACE PCR second |
Cassette AS | GGGGGGGGGGGGGATCTGAGTC | Cassette amplification |
Joint S | AGAGAAACCGTCTATCAGGGCGATGGC | TS-jPCR |
Joint AS | AGAGACCCTTTGACGTTGGAGTCCACG | TS-jPCR |