Evaluation method | Criteria | Points |
---|---|---|
BLAST e-value | < 0.001 | 1 |
Sequence identity between the query and the hit | identity 30% to 50%, coverage > 40% | 1 |
 | identity 50% to 70%, coverage > 40% | 2 |
 | identity 70% to 90%, coverage > 40% | 3 |
 | identity 90% to 99%, coverage > 40% | 4 |
 | identity > 99%, coverage > 40% | 5 |
BLAST alignment coverage for both query and hit | 60% to 80% | 1 |
 | 80% to 100% | 2 |
The query against the proteome associated with the hit | Best hit | 2 |
 | N/A | 1 |
The hit against the proteome associated with the query | Best hit | 2 |
 | N/A | 1 |