Table 2 Confidence score of homologs from Swiss-Prot database.
| Evaluation method | Criteria | Points |
|---|---|---|
| BLAST e-value | < 0.001 | 1 |
| Sequence identity between the query and the hit | identity 30% to 50%, coverage > 40% | 1 |
| identity 50% to 70%, coverage > 40% | 2 | |
| identity 70% to 90%, coverage > 40% | 3 | |
| identity 90% to 99%, coverage > 40% | 4 | |
| identity > 99%, coverage > 40% | 5 | |
| BLAST alignment coverage for both query and hit | 60% to 80% | 1 |
| 80% to 100% | 2 | |
| The query against the proteome associated with the hit | Best hit | 2 |
| N/A | 1 | |
| The hit against the proteome associated with the query | Best hit | 2 |
| N/A | 1 |
