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Table 3 Confidence score of predicted gene ontology terms

From: MESSA: MEta-Server for protein Sequence Analysis

Evaluation method Criteria Points
BLAST e-value 0.001 1
Sequence identity between the query and the hit identity 30% to 50%, coverage > 40% 1
  identity 50% to 70%, coverage > 40% 2
  identity 70% to 90%, coverage > 40% 3
  identity 90% to 100%, coverage > 40% 4
Alignment coverage for query and hit 60% to 80% 1
  80% to 100% 2
Evidence code of the GO term assigned to the hit EXP, IDA 3
  IPI, IMP, IGI, IEP, ISO, TAS 2
  ISS, ISA, ISM, IGC, IBA, IBD, IKR, IRD, RCA, NAS, IC, IEA 1
Consensus bonus Associated with no less than three hits 2
  1. EXP: inferred from experiment; GO: Gene Ontology; IBA: inferred from biological aspect of ancestor; IBD: inferred from biological aspect of descendant; IC: inferred by curator; IDA: inferred from direct assay; IEA: inferred from electronic annotation; IEP: inferred from expression pattern; IGC: inferred from genomic context; IGI: inferred from genetic interaction; IKR: inferred from key residues; IMP: inferred from mutant phenotype; IPI: inferred from physical interaction; IRD: inferred from rapid divergence; ISA: inferred from sequence alignment; ISM: inferred from sequence model; ISO: inferred from sequence orthology; ISS: inferred from sequence or structural similarity; NAS: non-traceable author statement; RCA: inferred from reviewed computational analysis; TAS: traceable author statement.
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