Response of N2 animals to thermal pulses of increasing amplitude. (A) Centroid speed of N2 animals plotted against time. The vertical red bar indicates duration of the pulse. The black line is the median speed profile of the animals at a given thermal pulse. The variability of speed at each time point is shown through boxplots. Temperature increases above the baseline (ΔT) as a result of thermal pulses are indicated. (B) Ethogram of different behavioral states of the N2 animals at indicated ΔT. The behavioral sequence of each animal over the duration of the assay (15 s) at a given ΔT is shown. Each row represents behavior of a single animal over time. Blue = forward state, red = reversal, black = pause, green = omega turns.