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Figure 4 | BMC Biology

Figure 4

From: The pupylation pathway and its role in mycobacteria

Figure 4

Dop and PafA are close structural homologs but catalyze distinct reactions. (a,b) The structures of Dop (a) and PafA (b) are shown in cartoon representation with ATP/ADP displayed in stick format. The catalyzed reactions are shown below the corresponding enzyme. For deamidation, R denotes a hydrogen; for depupylation or ligation, R denotes the protein lysyl moiety. Pup (dotted red line) is represented as binding in the putative Pup-binding groove with its carboxy-terminal glutamate (red, sticks) placed in the active site according to the position of glutamate in glutamyl-cysteine ligase (PDB: 2gwd). In PafA, Pup is displayed in its activated form corresponding to the phosphorylated intermediate shown in the scheme below (Pi is indicated by a red sphere).

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