Figure 4From: The pupylation pathway and its role in mycobacteriaDop and PafA are close structural homologs but catalyze distinct reactions. (a,b) The structures of Dop (a) and PafA (b) are shown in cartoon representation with ATP/ADP displayed in stick format. The catalyzed reactions are shown below the corresponding enzyme. For deamidation, R denotes a hydrogen; for depupylation or ligation, R denotes the protein lysyl moiety. Pup (dotted red line) is represented as binding in the putative Pup-binding groove with its carboxy-terminal glutamate (red, sticks) placed in the active site according to the position of glutamate in glutamyl-cysteine ligase (PDB: 2gwd). In PafA, Pup is displayed in its activated form corresponding to the phosphorylated intermediate shown in the scheme below (Pi is indicated by a red sphere).Back to article page