Table 1 Primers used in experiments
From: LoxP-FRT Trap (LOFT): a simple and flexible system for conventional and reversible gene targeting
Purpose |  | Sequence (5' → 3') |
|---|---|---|
Screening ES cells | Left arm F | CCGCCTACATTCTCCATCTTCTCCA |
| Â | Left arm R | CAGTCCCTTCCCGCTTCAGTGACAA |
| Â | Right arm F | TACGTATGGCACATAGAACTTGATA |
| Â | Right arm R | GAGCACCCAGTCCGCCCTGAGCAAA |
RT-PCR | Forward | CGGGACAAAGGGAAGCGAAG |
| Â | Reverse | CGCCACATGCCACCAATAATC |
Nested PCR for amplicons produced by RT-PCR | Forward | GGACAAAGGGAAGCGAAGCCGGAGCTG |
| Â | Reverse | GCCACATGCCACCAATAATCTTGCCAAT |
Production of probe for Southern blot | Forward | CCACTCCCCGTGGAACACGC |
| Â | Reverse | CCGTGACCCGGCTGTTGGTG |