The plasma membrane-resident Drosophila zinc transporters, dZips and dZnTs, are regulated by zinc availability. (A-B) Reverse transcriptase (RT)-PCR analysis of (A) dZips and (B) dZnTs in the gut showing transcriptional responsiveness of zinc transporters to dietary zinc. (C) Immunostaining reveals that dZip1 is upregulated by dietary zinc limitation at the midgut constriction (arrows ). Scale bars = 50 μm. (D,E) Western blotting analysis shows that (D) dZnT1 is downregulated by dietary zinc overload, but not by zinc limitation; and (E) using anti-HA antibody, shows that CG5130-HA is not responsive to dietary zinc level. NF, normal food; ZN, 2 mmol/l ZnCl2; EDTA, 0.3 mmol/l EDTA.