Human NOV - lacZ expressed in the basolateral amygdaloid nuclei, mid-cortical layers, and pyramidal neurons of the hippocampal formation. Expression analysis of the human NOV-lacZ strain was undertaken by examination of β-galactosidase (β-gal) staining (blue). (a,b) E12.5 whole and cleared embryos showed staining in the olfactory epithelium, and the primitive nasopharynx (black arrows). (c) P7 brains stained in the developing retromammillary nucleus (RM) (black arrows), and hippocampal formation (HPF) (white arrows). (d) Adult brains stained in the mid-cortical layers (black arrows), and the HPF (white asterisks). Staining was detected in the basolateral amygdaloid nuclei (BLP) (white arrows). (e) NOV-lacZ staining was also present in the adult RM (white arrows). (f) Colocalization experiment using β-gal staining and a neuronal nuclei (NeuN) antibody (brown) performed on adult brain cryosections revealed expression of NOV-lacZ in mature neurons in the cortical layers II, IV and VI. (g) Colocalization experiment revealed expression of NOV-lacZ in pyramidal neurons populating the HPF (NeuN, brown). Staining was found in the cell body in the cornu ammonis 1 region (CA1) (black arrow) and extended in the projections found in the stratum radiatum (SR). (h) Colocalization experiment revealed expression of NOV-lacZ in mature neurons (NeuN, brown) found in the BLP. Ctx, cortex. Scale bar: (a-e) 1 mm; (f,g) 50 μm; (h) 25 μm.