Table 1 Eight out of ten constructs successfully modified for expression-pattern characterization in mice
Brain region(s) | Gene | Plea | Parental BAC name | Construct size (bp) | Reporter | BAC name | Retrofitting status |
|---|---|---|---|---|---|---|---|
Thalamus | AMOTL1 | 5 | RP11-936P10 | 211,681 | lacZ | bEMS90 | Successful |
Brainstem, pons, medulla | GLRA1 | 91 | RP11-602K10 | 188,541 | N/A | bEMS89 | Failedb |
Hippocampus, dentate gyrus | LCT | 126 | RP11-406M16 | 156,570 | EGFP | bEMS75 | Successful |
Locus coeruleus | MAOA | 127 | RP11-475M12 | 197,191 | lacZ | bEMS84 | Successful |
Hippocampus, Ammon’s horn | NEUROD6 | 132 | RP11-463M14 | N/A | N/A | N/A | Failedc |
Basal nucleus of Meynert | NGFR | 133 | RP11-158L10 | 175,851 | lacZ | bEMS88 | Successful |
Amygdala, basolateral complex | NOV | 134 | RP11-840I14 | 202,342 | lacZ | bEMS91 | Successful |
Neurogenic regions | NR2E1 | 142 | RP11-144P8 | 138,165 | lacZ | bEMS86 | Successful |
Amygdala, basolateral complex | NR2F2 | 143 | RP11-134D15 | 213,112 | lacZ | bEMS85 | Successful |
Subthalamic nucleus | PITX2 | 158 | RP11-268I1 | 200,209 | lacZ | bEMS87 | Successful |