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Table 1 Eight out of ten constructs successfully modified for expression-pattern characterization in mice

From: Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice

Brain region(s) Gene Plea Parental BAC name Construct size (bp) Reporter BAC name Retrofitting status
Thalamus AMOTL1 5 RP11-936P10 211,681 lacZ bEMS90 Successful
Brainstem, pons, medulla GLRA1 91 RP11-602K10 188,541 N/A bEMS89 Failedb
Hippocampus, dentate gyrus LCT 126 RP11-406M16 156,570 EGFP bEMS75 Successful
Locus coeruleus MAOA 127 RP11-475M12 197,191 lacZ bEMS84 Successful
Hippocampus, Ammon’s horn NEUROD6 132 RP11-463M14 N/A N/A N/A Failedc
Basal nucleus of Meynert NGFR 133 RP11-158L10 175,851 lacZ bEMS88 Successful
Amygdala, basolateral complex NOV 134 RP11-840I14 202,342 lacZ bEMS91 Successful
Neurogenic regions NR2E1 142 RP11-144P8 138,165 lacZ bEMS86 Successful
Amygdala, basolateral complex NR2F2 143 RP11-134D15 213,112 lacZ bEMS85 Successful
Subthalamic nucleus PITX2 158 RP11-268I1 200,209 lacZ bEMS87 Successful
  1. aPleiades (Ple) promoter design number.
  2. bGLRA1 failed retrofitting with Hprt homology arms.
  3. cNEUROD6 failed BAC isolation from the library.
  4. BAC bacterial artificial chromosome, N/A not applicable.