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Table 2 Eight novel Hprt targeted embryonic stem cell lines successfully generated

From: Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice

Gene

Parental ESC

ESC clones

Final ESC line (mEMS)

Line (mEMS)

Genotype of parental ESC line

Number isolated

Correctly targeted (%)

AMOTL1

1202.04

B6129F1-Gt(ROSA)26Sor+/+, Hprtb-m3/Y

8

50

4645

LCT

1204.31

B6129F1-Gt(ROSA)26Sortm1Sor/+, Hprtb-m3/Y

31

48

3305

MAOA

1202.04

B6129F1-Gt(ROSA)26Sor+/+, Hprtb-m3/Y

15

53

4442

NGFR

1202.04

B6129F1-Gt(ROSA)26Sor+/+, Hprtb-m3/Y

20

20

4583

NOV

1202.04

B6129F1-Gt(ROSA)26Sor+/+, Hprtb-m3/Y

24

42

4521

NR2E1

1204

B6129F1-Gt(ROSA)26Sortm1Sor/+, Hprtb-m3/Y

29a

21

4751

NR2F2

1292.02

B6129F1-Aw-J/Aw, Gt(ROSA)26Sortm1Sor/+, Hprtb-m3/Y

25a

28

4990

PITX2

1202.04

B6129F1-Gt(ROSA)26Sor+/+, Hprtb-m3/Y

15

20

4496

  1. aObtained from multiple electroporations.
  2. ESC embryonic stem cell.
  3. mEMS (mammalian cell lines Elizabeth M. Simpson).