Skip to main content

Table 2 Eight novel Hprt targeted embryonic stem cell lines successfully generated

From: Non-coding-regulatory regions of human brain genes delineated by bacterial artificial chromosome knock-in mice

Gene Parental ESC ESC clones Final ESC line (mEMS)
Line (mEMS) Genotype of parental ESC line Number isolated Correctly targeted (%)
AMOTL1 1202.04 B6129F1-Gt(ROSA)26Sor+/+, Hprtb-m3/Y 8 50 4645
LCT 1204.31 B6129F1-Gt(ROSA)26Sortm1Sor/+, Hprtb-m3/Y 31 48 3305
MAOA 1202.04 B6129F1-Gt(ROSA)26Sor+/+, Hprtb-m3/Y 15 53 4442
NGFR 1202.04 B6129F1-Gt(ROSA)26Sor+/+, Hprtb-m3/Y 20 20 4583
NOV 1202.04 B6129F1-Gt(ROSA)26Sor+/+, Hprtb-m3/Y 24 42 4521
NR2E1 1204 B6129F1-Gt(ROSA)26Sortm1Sor/+, Hprtb-m3/Y 29a 21 4751
NR2F2 1292.02 B6129F1-Aw-J/Aw, Gt(ROSA)26Sortm1Sor/+, Hprtb-m3/Y 25a 28 4990
PITX2 1202.04 B6129F1-Gt(ROSA)26Sor+/+, Hprtb-m3/Y 15 20 4496
  1. aObtained from multiple electroporations.
  2. ESC embryonic stem cell.
  3. mEMS (mammalian cell lines Elizabeth M. Simpson).