Figure 6

MST1 promotes nuclear translocation of FOXO3a through phosphorylation at Ser207. A and C. Representative immunostaining using pan-FOXO3a and Ser207-phosphorylated FOXO3a antibodies on TA muscle sections obtained on denervation Day 3. B. At Day 3 after denervation, 41.46% ± 3.07% and 27.51% ± 2.28% of the total myofiber nucleus were stained with FOXO3a in WT and MST1 deficient TA muscles, respectively. D. At Day 3 after denervation, 32.63% ± 1.82% and 12.10% ± 1.49% of total TA muscle nuclei stained with serine 207-posphorylated FoxO3a in WT and MST1 mice, respectively. *** P < 0.0001 by two-tailed Student's test..