Figure 2

Subcellular localization of CdICE1 protein in onion epidermal cells. (a–c) Onion epidermal cells transformed with 35S::GFP as a control; (d–g) Onion epidermal cells transformed with 35S::CdICE1-GFP. Photographs were taken (a, d) under dark-field for green fluorescence; e (b, f) under bright-field for the morphology of the cells; (e, DAPI staining) under dark field for blue fluorescence; and (c, g) in combination. Bar = 50 μm. DAPI, 4',6-diamidino-2-phenylindole.