Figure 7

Quantitative reverse transcription PCR (qRT-PCR) analysis and comparison of freezing tolerance between WT and mutant Arabidopsis . (a) matured miR398 expression under acclimation at 16°C and 4°C; (b) CSD1 and CSD2 expression at 16°C; (c) qRT-PCR analysis of primary transcript MIR398 a, b and c during acclimation at 16°C; (d) RT-PCR analysis of expression of MIR398c, CSD1 and CSD2 between WT Arabidopsis and the mutants; (e) Arabidopsis treated at −9°C after 21 days of recovery growth following 16°C/4°C/non-acclimation conditions; (f) Electrolyte leakage (EL) of Arabidopsis leaves at 1 hour after treatment at −9°C. WT, wild type.