IpsA plays an essential role in the integrity of the cell wall of Corynebacteriales . (A) Current model of the structure of the cell wall of Corynebacteria and Mycobacteria. (B) Comparison of the organization of the IpsA genome locus in C. glutamicum and related species. IpsA and homologous proteins are shown in black. Data were taken from [5, 6]. The percentage identity of the amino acid sequences to IpsA from C. glutamicum was taken from NCBI Blast. hp, hypothetical protein. (C, D) Growth curves (C) and phenotype (D) of C. glutamicum wild-type and ΔipsA. Results in (C) show the growth (backscatter) from sequential cultures in minimal medium with glucose or myo-inositol as carbon source. After reaching the stationary phase, the cells were diluted into fresh medium at an initial OD600 of 1. Shown are three glucose cultures and the third myo-inositol culture (n = 3). In (D), DNA was stained with 4’,6-diamidino-2-phenylindole (DAPI) (cyan) and lipophilic regions with nile red (red). The scale bar is 5 μm.