Figure 5

Absence of extended oocyte proliferation in embryonic Amhr2-Cre/+;Notch2^flox/- ovaries. Pregnant mice were injected with 5-bromo-2'-deoxyuridine (BrdU) twice daily between E16.5 through E18.5 (a total of six injections). Ovaries were isolated from Amhr2-Cre/+;Notch2^flox/- and control littermate progeny on the day of birth (postnatal day 0; PND0), and sections were stained with anti-Vasa (red) antibodies to label oocytes, and with anti-BrdU (green) antibodies to identify proliferating cells. Sections were also counterstained with DAPI (blue) to stain cell nuclei. As shown in the merged figure on the right, at PND0, no oocyte in either (A) littermate control or (B) mutant ovaries had incorporated BrdU as a result of these injections. (C) Higher magnification view of the mutant ovary (boxed region in B).