Inference of burst size and closed time from mean and normalized variance (NV) of protein levels. (A) At steady states, using the best transcription-translation parameter set (ρ, , γ, and α) and the modified Paulsson's equation system, the mean closed time could be calculated from the protein mean and protein NV (red grid). (B) Using the same data and equation system as in panel (A), the mean burst size could be calculated from the protein mean and protein normalized variance (red grid). Note that grids of both panels are linked because each value pair (protein mean and NV) corresponds to a single value pair (mean burst size and mean closed time). For both parts, clones C1, C3, C5, C7, C11, and C17 are represented as blue points on the grid, and all axes are on a logarithmic scale.