Secondary screen validation of top hits. NF-κB -luc cells were co-transfected with the indicated miRNAs and a constitutively expressed Renilla reporter plasmid. Firefly luciferase activity was normalized to that of Renilla luciferase. All values are means ± SD. n = 3. *P ≤0.05, **P ≤0.01. miRNAs, micro RNAs; NF-κB, nuclear factor kappa-B; NF-κB-luc, NF-κB responsive luciferase reporter; SD, standard deviation.