The number of PGCs was reduced in GR-deficient embryos. PGCs (black arrow-heads) were labeled with Stella (brown signal). At E10.5, Stella-positive PGCs were observed in the mesenchyme under the coelomic epithelium in both control (A) and Wt1R394W/R394W (D) embryos, and no difference in PGC number was noted between control and Wt1R394W/R394W embryos. In control embryos, Stella-positive PGCs colonized the developing gonads at E11.5 (B) and E12.5 (C), and the number of the PGCs increased rapidly. In contrast, in Wt1R394W/R394W embryos, the PGCs were widely scattered in the mesenchyme under the coelomic epithelium at E11.5 (E) and E12.5 (F). Histogram summarizing selected PGCs number in microscopic cross sections of three independent embryos. Numbers in parentheses show the actual number of PGCs counted at E10.5 (G), E11.5 (I) and E12.5 (K). The average number of PGCs was significantly reduced in Wt1R394W/R394W embryos compared with control embryos at these stages (H, K, L). * P <0.05. ce, coelomic epithelium; gr, genital ridge; g, gonad. Scale bars: 50 μm.