Steel factor, Sdf1 and Integrinβ1 expression were unchanged in GR-deficient embryos. The expression patterns of Steel factor, Sdf1 and Integrinβ1 in E10.5 control and Wt1R394W/R394W embryos were assessed by immunostaining. Sdf1 was expressed in the urogenital region of both control (A) and Wt1R394W/R394W (B) embryos. High-magnification images showed that the expression of Sdf1 in the coelomic epithelium (white arrows) was not obviously changed in Wt1R394W/R394W (D) compared with control embryos (C). Steel factor was detected in the mesentery of control (E) and Wt1R394W/R394W (F) embryos. G and H are high-magnification images from E and F, respectively. Integrinβ1 was widely expressed in both control (I) and Wt1R394W/R394W (J) embryos. K and L are high-magnification images of I and G, respectively. da, dorsal artery; gr, genital ridge; hg, hindgut; mes, mesentery. Scale bars: 50 μm. The mRNA level of c-Kit, Steel, Cxcr4, Sdf1, Cdh1 and Integrinβ1 was detected by real-time RT-PCR (M), and normalized against Gapdh. Each bar, mean ± SD of n = 3 experiments.