The PGCs identity was not changed in Wt1R394W/R394W embryos. PGCs identity was examined by double staining of Stella (red) with PGCs characteristic markers, including Oct4 (A), Dazl (B), SSEA-1 (C), and Blimp1 (D). In Wt1R394W/R394W embryos, PGCs still expressed Stella, SSEA-1, Blimp1, Dazl and pluripotent marker Oct4. Scale bars: 50 μm. PGCs-PGCs interactions, such as E-cadherin and β-catenin were maintained in Wt1R394W/R394W embryos (E, F). Scale bars: 50 μm. The mRNA level of PGCs characteristic markers (Oct4, Dazl, SSEA-1, Blimp1, Nanos2, Figla), and PGCs-PGCs interaction markers (E-cadherin and β-catenin) were detected by real-time RT-PCR, and normalized against PGC-specific marker Stella (G). Each bar, mean ± SD of n = 3 experiments.